• Title/Summary/Keyword: Three-dimensional culture

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The Characteristics of Color on Korean Costume by Basic Culture (기층문화를 통한 한국복식의 색채 특성 연구)

  • Kim Ji-Young;Kim Young-In
    • Journal of the Korean Society of Costume
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    • v.56 no.5 s.104
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    • pp.29-43
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    • 2006
  • The purpose of this study is to examine a unique characteristic of the colors of the costumes in Korean basic culture in the aim of seeking the characteristics and the conceptual meanings of colors found in the majority’s culture. The scope of the basic culture was divided into folk belief, folk game and folk play. Within these limits, the colors of the dress, accessories, instruments were extracted by comparing with the naked eye in NCS Color System. For the analysis of hue and tone, the secondary dimensional analysis using NCS color system and the three-dimensional analysis using the software, COLOR 3D Version 2.0, were done. The result of this investigation is that the colors of the costume in the Korean basic culture are white, gray and black of achromatic color and yellow, yellowish red and purplish blue. This confirms that the colors based on Five-elements color are becoming the basis too basic culture. And Arche-pattern, which is a characteristic commonly found in the Korean traditional society, was shown as a characteristic of color. The colors of the costumes in Korean basic culture are uniquely adopted by the Korean civilians according to their religious and philosophical living standard. This study is meaningful in seeking a root for the formation of their unique color culture.

implementation of 3D Reconstruction using Multiple Kinect Cameras (다수의 Kinect 카메라를 이용한 3차원 객체 복원 구현)

  • Shin, Dong Won;Ho, Yo Sung
    • Smart Media Journal
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    • v.3 no.4
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    • pp.22-27
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    • 2014
  • Three-dimensional image reconstruction allows us to represent real objects in the virtual space and observe the objects at arbitrary view points. This technique can be used in various application areas such as education, culture, and art. In this paper, we propose an implementation method of the high-quality three-dimensional object using multiple Kinect cameras released from Microsoft. First, We acquire color and depth images from triple Kinect cameras; Kinect cameras are placed in front of the object as a convergence form. Because original depth image includes some areas where have no depth values, we employ joint bilateral filter to refine these areas. In addition to the depth image problem, there is an color mismatch problem in color images of multiview system. In order to solve it, we exploit an color correction method using three-dimensional geometry. Through the experimental results, we found that three-dimensional object which is used the proposed method is more naturally represented than the original three-dimensional object in terms of the color and shape.

Immortalization of Primary Keratinocytes and Its Application to Skin Research

  • Choi, Moonju;Lee, Choongho
    • Biomolecules & Therapeutics
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    • v.23 no.5
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    • pp.391-399
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    • 2015
  • As a major component of the epidermal tissue, a primary keratinocyte has served as an essential tool not only for the study of pathogenesis of skin-related diseases but also for the assessment of potential toxicities of various chemicals used in cosmetics. However, its short lifespan in ex vivo setting has been a great hurdle for many practical applications. Therefore, a number of immortalization attempts have been made with success to overcome this limitation. In order to understand the immortalization process of a primary keratinocyte, several key biological phenomena governing its lifespan will be reviewed first. Then, various immortalization methods for the establishment of stable keratinocyte cell lines will be explained. Finally, its application to a three-dimensional skin culture system will be described.

Engineering of a Human Skin Equivalent

  • Ghalbzouri Abdoelwaheb El
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.29 no.2 s.43
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    • pp.105-130
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    • 2003
  • Human skin equivalents, also designated as cultured skin substitute (Boyce and Warden, 2002) or organotypic co-cultures (Maas-Szabowski et al., 1999, 2000, 2003), are three-dimensional systems that are engineered by seeding fibroblasts into a three-dimensional dermal matrix. Such a dermal equivalent is then subsequently seeded with human keratinocytes. After cell attachment, the culture is kept first under submerged condition to allow keratinocyte proliferation. Thereafter, the culture is lifted the air-liquid interface (A/L) to expose the epidermal compartment to the air, and to further induce keratinocyte differentiation. During the air-exposure, nutrients from the medium will diffuse through the underlying dermal substrate towards the epidermal compartment and support keratinocyte proliferation and differentiation. Under these conditions, a HSE is formed that shows high similarity with the native tissue from which it was derived (Figure 1) (Bell et at., 1981; Boyce et al., 1988; Ponec et al., 1997;El Ghalbzouri et al.., 2002).

A Study On The Comic Presentation For Three-Dimensional Shot (코믹연출을 위한 입체적인 샷(Shot)연구)

  • Hwang, Kil-Nam
    • Proceedings of the Korea Contents Association Conference
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    • 2006.11a
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    • pp.836-839
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    • 2006
  • In making animation product, it is utmost important to produce comic scene using emphasis and exaggeration. This study aims to research on the comic presentation of animation through application of exaggeration and prominence. First, it suggests going under image extraction experiment and moving-shot-method as a mode of measuring three dimensional shot. Moreover, an analysis of the product of three dimensional shot of animation suggests a comic effect.

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Effect of Three Dimensional Culture of Porcine Endometrial Cells on Their Plasminogen Activity and Pre-implantation Embryo Development after Co-culture (돼지 자궁 세포의 3차원 배양이 Plasminogen 활성과 수정란 발육에 미치는 영향)

  • Lee, Sang-Hee;HwangBo, Yong;Cha, Hye-Jin;Kim, Su-Ji;Kim, Min-Gyeong;Cheong, Hee-Tae;Yang, Boo-Keun;Park, Choon-Keun
    • Journal of Embryo Transfer
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    • v.29 no.3
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    • pp.207-219
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    • 2014
  • Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used various experiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3D cultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrial and myometrial cells. The most rapid growth of stromal cells were confirmed in $2.5{\times}10^5cells/ml$ and gel matrix containing 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) were higher than without hCG, but, there are not significant difference among the treatment. On the other hand, expression of uPA after treatment of $IL-1{\beta}$ (0.1, 1, 10 and 100 ng/ml) were higher than without $IL-1{\beta}$, but, there are not significant difference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PA activity was significantly decreased (p<0.05). Blastocyst was producing in PZM-3 medium containing FBS and endometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavage rates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culture system. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivo physiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that 3D culture system with embryo co-culture need continuous research.

A production process of cruiser sailing boat based on the three dimensional hull design (3차원 설계를 적용한 크루저급 세일링보트의 제작 공정)

  • Park, Gen-Ong;Kim, Dong-Joon;Park, Jong-Heon
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.44 no.4
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    • pp.353-361
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    • 2008
  • Recently a modern style sailing boat suitable for Korean sea was designed through full three dimensional design skill by the authors. In this paper, based on this three dimensional hull and deck design, a production process of 31ft class cruiser sailing boat was developed. First of all, it was possible to make the digital mock-up for design boat. Through this mock-up and RP(rapid prototyping) modeling, an appropriate general arrangement of design boat was able to be determined at final. And also the female deck mould was able to be made by a 5-axis NC cutting machine. By doing this method, more higher efficiency and precision for sailing boat production could be achieved than before. Through this research the total process of design and construction for the designed boat was established.

Fabrication and validation study of a 3D tumor cell culture system equipped with bloodvessle-mimik micro-channel (혈관모사 마이크로채널이 장착된 3D 종양 세포 배양 시스템의 제작 및 검증 연구)

  • Park, Jeong-Yeon;Koh, Byum-seok;Kim, Ki-Young;Lee, Dong-Mok;Yoon, Gil-Sang
    • Design & Manufacturing
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    • v.15 no.2
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    • pp.11-16
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    • 2021
  • Recently, three-dimensional (3D) cell culture systems, which are superior to conventional two-dimensional (2D) vascular systems that mimic the in vivo environment, are being actively studied to reproduce drug responses and cell differentiation in organisms. Conventional two-dimensional cell culture methods (scaffold-based and non-scaffold-based) have a limited cell growth rate because the culture cannot supply the culture medium as consistently as microvessels. To solve this problem, we would like to propose a 3D culture system with an environment similar to living cells by continuously supplying the culture medium to the bottom of the 3D cell support. The 3D culture system is a structure in which microvascular structures are combined under a scaffold (agar, collagen, etc.) where cells can settle and grow. First, we have manufactured molds for the formation of four types of microvessel-mimicking chips: width / height ①100 ㎛ / 100 ㎛, ②100 ㎛ / 50 ㎛, ③ 150 ㎛ / 100 ㎛, and ④ 200 ㎛ / 100 ㎛. By injection molding, four types of microfluidic chips were made with GPPS (general purpose polystyrene), and a 100㎛-thick PDMS (polydimethylsiloxane) film was attached to the top of each microfluidic chip. As a result of observing the flow of the culture medium in the microchannel, it was confirmed that when the aspect ratio (height/width) of the microchannel is 1.5 or more, the fluid flows from the inlet to the outlet without a backflow phenomenon. In addition, the culture efficiency experiments of colorectal cancer cells (SW490) were performed in a 3D culture system in which PDMS films with different pore diameters (1/25/45 ㎛) were combined on a microfluidic chip. As a result, it was found that the cell growth rate increased up to 1.3 times and the cell death rate decreased by 71% as a result of the 3D culture system having a hole membrane with a diameter of 10 ㎛ or more compared to the conventional commercial. Based on the results of this study, it is possible to expand and build various 3D cell culture systems that can maximize cell culture efficiency by cell type by adjusting the shape of the microchannel, the size of the film hole, and the flow rate of the inlet.

The Effects of Consumers' Attitude toward Party on Dress Code Receptiveness (파티에 대한 태도가 드레스코드 수용에 미치는 영향)

  • Seo, Sang-Woo;Lee, Yu-Ri
    • Journal of the Korean Society of Costume
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    • v.58 no.1
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    • pp.104-115
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    • 2008
  • Party is an important communication method in the 21st century and this tendency is not exceptional in Korea. With this noteworthy phenomenon, this study summarized the concept and history of party first, and then analysed "belief - attitude - behavioral intention" model based on the one-dimensional attitude theory. The variables included in this research were western culture familiarity as belief factor, three variables evaluating the party culture(i.e., non-popularity of party, marketability of party, sociability of party) as attitude factor, and dress code receptiveness as behavioral intention factor. The samples of this study were 498 women in twenties and thirties from metropolitan areas in South Korea. SPSS 12.0 and AMOS 4.0 packages were used for statistical analysis. The results of this research are as follows: First, western culture familiarity had a significant effect on attitude toward party culture. Specifically, western culture familiarity negatively influenced the non-popularity dimension of party and positively influenced the marketability and sociability dimension of party. Second, attitude toward party culture significantly influenced the dress code receptiveness. Consecutively, all of three dimensions about attitude toward party culture positively influenced the dress code receptiveness. Managerial implications were provided.

Strategic Culture Marketing Enhancing Brand Image and Customers' Loyalty - Focusing on Multi-dimensional approach for Companies, Consumers, and Other Subjects - (브랜드의 이미지와 충성도를 높이는 문화마케팅 전략에 관한 연구 -기업, 소비자, 지원대상의 다차원적 접근을 중심으로-)

  • Lee, Yun-Kyung;Shin, Kyu-Hee;Hwang, Sun-Jin
    • Journal of the Korean Society of Costume
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    • v.59 no.5
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    • pp.167-179
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    • 2009
  • The purposes of this study were to explore the influence on fashion brand image and brand loyalty according to fit of the fashion brand with cultural art field, motivation in culture marketing of the apparel company, and involvement of the consumers in art activities. The subjects ware 274 male and female in 20s and 30s living in Seoul and Gyeonggi area. The study was obtained through the use of an experimental design which was $2{\times}[2{\times}2]$ mixed factorial design. The summary main results were as follows. First, as far as the effect of the three variables on the social contribution image of the fashion brand, the results of the 3-way interaction are positive. Second, as far as the effect of the three variables on the product image of the fashion brand, the data suggests that only the main-effect of motivation in culture marketing of the apparel company is meaningfully positive. Third, as far as the effect of the three variables on fashion brand loyalty, both fit of the fashion brand with cultural art field and involvement of the consumers in art activities variables show positive results.