• Journal of Periodontal and Implant Science
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• 제32권1호
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• pp.129-142
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• 2002

Epithelial-mesenchymal interaction plays a important role in cell growth and differentiation. This interaction is already well known to have an importance during the organ development as well as cell growth and differentiation. However, in vitro experimental model is not well developed to reproduce in vivo cellular microenvironment which provide a epithelial-mesenchymal interaction. Because conventional monolayer culture lacks epithelial-mensenchymal interaction, cultivated cells have an morphologic, biochemical, and functional characteristics differ from in vivo tissue. Moreover, it's condition is not able to induce cellular differention due to submerged culture condition. Therefore, the aims of this study were to develop and evaualte the in vitro experimental model that maintains epithelial-mesenchymal interaction by organotypic raft culture, and to characterize biologic properties of three-dimensionally reconstituted oral keratinocytes by histological and immunohistochemical analysis. The results were as follow; 1. Gingival keratinocytes reconstituted by three-dimensional organotypic culture revealed similar morphologic characteristics to biopsied patient specimen showing stratification, hyperkeratinosis, matutation of epithelial architecture. 2. Connective tissue structure was matured, and there is no difference during stratification period of epithelial 3-dimensional culture. 3. The longer of air-exposure culture on three-dimensionally reconstituted cells, the more epithelial maturation, increased epithelial thickness and surface keratinization 4. In reconstitued mucosa, the whole epidermis was positively stained by anti-involucrin antibody, and there is no difference according to air-exposured culture period. 5. The Hsp was expressed in the epithelial layer of three-dimensionally cultured cells, especially basal layer of epidermis. The change of Hsp expression was not significant by culture stratification. 6. Connexin 43, marker of cell-cell communication was revealed mild immunodeposition in reconstitued epithelium, and there is no significant expression change during stratification. These results suggest that three-dimensional oragnotypic co-culture of normal gingival keratinocytes with dermal equivalent consisting type I collagen and gingival fibroblasts results in similar morphologic and immunohistochemical characteristics to in vivo patient specimens. And this culture system seems to provide adequate micro-environment for in vitro tissue reconstitution. Therefore, further study will be focused to study of in vitro gingivitis model, development of novel perioodntal disease therapeutics and epithelial-mensenchymal interaction.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2001년도 추계학술대회 논문집
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• pp.31-35
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• 2001

Three dimensional dynamic cutting can be postulated as an equivalent orthogonal dynamic cutting through the plane containing both the cutting vector and the chip flow velocity vector in cutting process. An analytical expression of dynamic cutting force is obtained from the cutting parameters determined by the static three dimensional cutting experiments. Particular attention is paid to the energy supplied to the vibration of the tool behind the vertical vibration and the direction. The phase lag of the horizontal vibration of the tool behind the vertical vibration and the direction angel of the fluctuating cutting force must be regarded in point of stability limits. Chatter vibration can effectively be suppressed by enlarging the dynamic rigidity of the cutting system in the vertical cutting force direction. A good agreement is found between the stability limits predicted by theory and the critical width of cut determined by experiments.

• 전산구조공학
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• 제8권1호
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• pp.147-160
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• 1995

종속변수와 기본 탄성방정식의 가중잔차 근사식의 Fourier 급수 전개를 이용한 3차원 판이론을 제시하였다. 판의 가중잔차 평형방정식은 가중된 변위로 표시되며, 그 결과는 다시 위치에너지 Functional을 이용하여 유한요소해석을 수행하였다. 본 해석은 Strip판에 적용되어 2가지 예를 분석하였으며, 예제의 결과는 이론해와 잘 일치하였다.

• 한국정밀공학회지
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• 제10권4호
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• pp.216-226
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• 1993

Three dimensional cutting is considered as an equivalent orthogonal cutting through the plane containing both the cutting velocity vector and the chip flow velocity vector in dynamic cutting process. An analytical expression of dynamic cutting force is obtained from the cutting parameters determined by the static cutting. Particular attention is paid to the energy supplied to the vibratory system of cutting tool with two degree of freedom. In this approach, the phase lag of the horizontal vibration of the tool behind the vertical vibration and the direction angle of the fluctuating cutting force is considered in point of stability limits. Chatter vibration can be effectively suppressed by relatively increasing the spring constant and the damping coefficient of the cutting system in the vertical cutting force direction. A good agreement is found between the stability limits predicted by theoretical value and experimental results.

• International Journal of Stem Cells
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• 제17권3호
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• pp.330-336
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• 2024

Mesenchymal stem cells in the dental tissue indicate a disposition for differentiation into diverse dental lineages and contain enormous potential as the important means for regenerative medicine in dentistry. Among various dental tissues, the dental pulp contains stem cells, progenitor cells and odontoblasts for maintaining dentin homeostasis. The conventional culture of stem cells holds a limit as the living tissue constitutes the three-dimensional (3D) structure. Recent development in the organoid cultures have successfully recapitulated 3D structure and advanced to the assembling of different types. In the current study, the protocol for 3D explant culture of the human dental pulp tissue has been established by adopting the organoid culture. After isolating dental pulp from human tooth, the intact tissue was placed between two layers for Matrigel with addition of the culture medium. The reticular outgrowth of pre-odontoblast layer continued for a month and the random accumulation of dentin was observed near the end. Electron microscopy showed the cellular organization and in situ development of dentin, and immunohistochemistry exhibited the expression of odontoblast and stem cell markers in the outgrowth area. Three-dimensional explant culture of human dental pulp will provide a novel platform for understanding stem cell biology inside the tooth and developing the regenerative medicine.

• 패션비즈니스
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• 제20권2호
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• pp.124-133
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• 2016

Currently the fashion industry is developing to create a novel culture due to the very sensitive and knowledge-oriented advancement of the IT industry. With fast turnover of information, consumers have come to have a more diverse desire for purchasing. Cubical expression techniques, which empathizes formativeness, can be a creative expression method adjusting into the trend of this era. Along with functional aspects of consumers, even in a textile manufacturing sector, new materials are required to meet sensitive and emotional aspects. Consumers' desire for new and creative designs and the development and adoption of new materials are essential to meet their emotions. The IT industry and fashion industry are forced to combine and a 3D apparel CAD system has been developed, enabling virtual clothing to be represented within a computer virtual space. All processes such as design, pattern creation, sewing and simulation are possible in 3D level. Digital clothing can shorten the production process time and is very effective in that it can reduce clothing waste generated during the sample production. This paper reviewed the works of Dutch designer, Iris van Herpen, who has developed formative designs. She tries to build, construct, and sculpt employing diversified materials other than soft textile materials, as shown in her series of fashion shows. The materials include films, 3D printed polymers, stiff and sheer organza, and artificial leather textiles. A few characteristics of her works have been selected in order to prepare patterns exhibiting the traits. The paper further focused on the physical features of the textile materials used to express similar techniques and its various forms were reviewed.

• 한국의상디자인학회지
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• 제26권1호
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• pp.61-76
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• 2024

The purpose of this study is the development of an experimental design that aims to implement three-dimensional fashion design by observing the human body, extracting and combining geometric shapes and forms, and focusing on attempts to decompose the geometry of the human body in art history. Considering the characteristics of fashion design, which inevitably reflect human images visually, this study considered works by deriving geometric shapes and forms of the human body and focusing on decomposition and combination to apply them to fashion design. The results obtained through the development of fashion design through decomposition and combination based on geometric human body analysis are as follows. First, geometric analysis of the human body as an object of expression continues from the history of Cubism to modern fashion design. Second, the geometric shapes of the human body that appear in contemporary fashion design maximize visual effects through three-dimensional composition, emphasizing simplicity while showing originality through various expressions. Third, when exploring the geometric shapes of a moving human body, it was possible to extract a wide variety of shapes and forms through drawing and simplifying the human body's movements. Fourth, the formative method of fashion design was introduced and used for the aesthetic combination of objects for fashion design through decomposition and combination. This study was able to show unique and diverse combinations of visually concise and ordered geometric shapes in the expression of fashion design by decomposing and combining them. The significance of these geometric forms is that they can diversify formative informativeness in the expression of fashion design with modern compositional beauty.

• 한국가구학회지
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• 제14권2호
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• pp.75-86
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• 2003

The purpose of the study is to explore the possibilities for translating the images and meaning of Chinese characters into furniture. Using Chinese characters as designing sources for furniture has a lot of possibilities to inspire originative and creative works. When using images of characters, totally peculiar and new shapes of furniture which have great esthetic value can be considered. The functions of furniture are related to the meanings of characters. Therefore, the furniture could be recognized by the characters from which the works were formed. In the process of interpreting two dimensional letters in three dimensional forms, the graphic elements and values of the characters as two dimensional objects should be analyzed. In order to express the characters completely, both esthetic shapes and the essences of characters should be blended. For the effective translating work, the method of expression may require exaggerating, simplifying and abbreviating the shapes of characters.

• 한국의상디자인학회지
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• 제12권2호
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• pp.107-118
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• 2010

The purpose of this study is to be data and to help understanding of pattern of Korean, Chinese and Japanese traditional dress. The methods of this study are the study of academic literatures as well as practical study through the analysis of case studies about actual works. The summary of this study's results is like followings. First, standard of Pattern are nature, mam-made in motif object, Naturalistic, Geometric, Stylized, Abstract in motif express, Order, Disorder in pattern express. Second, a point of sameness of motif object in traditional dress of three nations were that nature and letter abstractive of mam-made is a lot. but motif of Japan was used in daily life motif of mam-made which was not in Korea and China Third, commonly, motif expression of three nations were developed Naturalistic, Stylized. but in Naturalistic, Korea was simple, plane, China was three-dimensional, Japan was expressed super realism. Fourth, as formative aesthetic of Pattern, Korea is natural, plane, simple and symbolic, China is gorgeous, three-dimensional, immaculacy, filling and symbolic, Japan is delicate, complicated, decoration and symbolic.

• 한국실내디자인학회논문집
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• 제39호
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• pp.20-27
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• 2003

The purpose of this study is to provide a practical guide for selecting architectural artwork and analyze relationship between artwork and interior space & facade. The problems with many outdoor artwork did not make the characteristics of the specific environment and did not form a big flow in the urban contextualism. Choosing the artwork, architectural environment for artwork is very much part of the designers' task. When artwork is to be specifically acquired for a particular projects, designer and client must work together to make choices and plan placement. The data are collected through evaluation surveys of college students and graduate students majoring in interior architecture & design, and analyzed by using SPSS-WIN program to find the major characterized factors. It is concluded that architectural artwork should be constituted with nine major factors of interior space - theme/scale, situation/contemporay accord, finished materials harmony, line/two-three dimensional form, proportion/rhythm, sequence, natural/artificial form, abstract/reality expression, originality - and eight major factors of facade - situation, abstract/reality expression, finished materials harmony, theme/sequence, natural/artificial form, proportion/rhythm, static/dynamic expression, originality/. contemporay accord.