• Title/Summary/Keyword: Three Rs

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Sequence and Phylogenetic Analysis of Respiratory Syncytial Virus Isolated from Korea (국내에서 유행한 Respiratory Syncytial 바이러스의 염기서열 및 계통분석)

  • Kwon, Soon-Young;Choi, Young-Ju;Kim, So-Youn;Song, Ki-Joon;Lee, Yong-Ju;Choi, Jong-Ouck;Seong, In-Wha
    • The Journal of Korean Society of Virology
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    • v.26 no.1
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    • pp.9-22
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    • 1996
  • Respiratory Syncytial virus (RSV) is an important cause of acute lower respiratory tract infections in human, with infants and young children being particularly susceptible. In the temperate zones, sharp annual outbreaks of RSV occur during the colder months, in both the northern and the southern hemisphere. RSV is unusual in that it can repeatedly reinfect individuals throughout life and infect babies in the presence of maternal antibody. RSV isolates can be divided into two subgroups, A and B, on the basis of their reactions with monoclonal antibodies, and the two subgroups are also distinct at the nucleotide sequence level. The specific diagnosis of RSV infection was best made by isolation of virus in tissue culture, identification of viral antigen, or by specific serologic procedures. Recently, rapid detection of RSV and analysis of RSV strain variation became possible by development of methods of reverse transcription and polymerase chain reaction amplification. In this study, to determine the genetic diversity of RSV found in Korea, 173 bp and 164 bp spanning selected regions of the RSV F and SH genes were enzymatically amplified and sequenced, respectively. Eight for F gene and three for SH gene were detected in 66 nasopharyngeal swap samples tested. Two major antigenic subgroups, A and B were confirmed from Korean samples (seven for subgroup A and one for subgroup B). At the nucleotide level of the F gene region, Korean subgroup A strains showed 95-99% homologies compared to the prototype A2 strain of subgroup A and 93-100% homologies among Korean subgroup A themselves. For the SH gene region, Korean subgroup A strain showed 97.5% homology compared to the prototype A2 strain of subgroup A, and Korean subgroup B strain showed 97% homology compared to the prototype 18537 strain of subgroup B. Most of base changes were transition and occured in codon position 3, which resulted in amino acid conservation. Using the maximum parsimony method, phylogenetic analysis indicated that Korean RSV strains formed a group with other RSV strains isolated from the United States, Canada, the Great Britain and Australia.

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Effects of Red and Green Glassworts (Salicornia herbacea L.) on Physicochemical and Textural Properties of Reduced-salt Cooked Sausages

  • Kim, Hyun-Wook;Hwang, Ko-Eun;Song, Dong-Heon;Kim, Yong-Jae;Ham, Youn-Kyung;Yeo, In-Jun;Jeong, Tae-Jun;Choi, Yun-Sang;Kim, Cheon-Jei
    • Food Science of Animal Resources
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    • v.34 no.3
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    • pp.378-386
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    • 2014
  • This study was conducted to determine the effects of red and green glasswort on the physicochemical and textural properties of reduced-salt cooked sausages. The control was formulated with 1.5% NaCl; then, three reduced-salt treatments were prepared, with 0.75% NaCl (RS), 0.75% NaCl+1.0% red glasswort (RSR) and 0.75% NaCl+1.0% green glasswort (RSG), respectively. The addition of glasswort within the added amount of 1% had no influence on the pH value of the reduced-salt cooked sausages, regardless of the glasswort type. In terms of color, RSG treatment conveyed a higher hue angle value than the RSR treatment (p<0.05). Increases in the protein solubility (total and myofibrillar proteins) and apparent viscosity of reduced-salt meat batter that were due to the addition of glasswort were observed; however, there were no differences according to the type of glasswort (p>0.05). Furthermore, the addition of glasswort, regardless of its type, resulted in decreased cooking loss, and increased emulsion stability. As a result, reduced-salt cooked sausages formulated with either red or green glasswort demonstrated similar textural properties to those of the control. In conclusion, the type of glasswort within an added amount of 1% had no influence on the physicochemical and textural properties of reduced-salt cooked sausages, except for the color characteristics. In terms of color alteration by the addition of glasswort, the red glasswort, which in comparison with the green glasswort could minimize the color changes of reduced-salt cooked sausages, might be an effective source for manufacturing meat products.

Effect of Fiber Type and Combination on the Reinforcement of Heat Polymerized Denture Base Resin (섬유의 종류와 조합이 열중합 의치상 레진의 강화에 미치는 영향)

  • Yu, Sang-Hui;Kim, Young-Im
    • Journal of dental hygiene science
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    • v.10 no.6
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    • pp.445-450
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    • 2010
  • The aim of this study was to evaluate the effect according to the fiber type and combination on the reinforcement of heat-polymerized denture base resin. The heat-polymerized resin(Vertex RS, Dentimax, Netherlands) was used in this study. Glass fiber(GL; ER 270FW, Hankuk Fiber Glass, Korea), polyaromatic polyamide fiber(PA; aramid; Kevlar-49, Dupont, U.S.A.) and ultra high molecular weight polyethylene fiber(PE, polyethylene; P.E, Dong Yang Rope, Korea) were used to reinforce the denture base resin specimens. The final size of test specimen was $64mm{\times}10mm{\times}3.3mm$. The specimens of each group were stored in distilled water at $37^{\circ}C$ for 50 hours before measurement. The flexural strength and flexural modulus were measured by an universal testing machine(Z020, Zwick, Germany) at a crosshead speed of 5 mm/min in a three-point bending mode. In this study, all fibers showed reinforcing effects on denture base resin(p<0.05). In terms of flexural strength and flexural modulus, glass fiber 5.3 vol.% showed most effective reinforcing effect on heat polymerized denture base resin. For flexural modulus, PA/GL was the highest in denture base resin specimen for hybrid FRC using two combination (p<0.05). Glass fiber 5.3 vol.% and PA/GL are considered to be applied effectively in reinforcing the heat polymerized denture base resin.

Exploring Feature Selection Methods for Effective Emotion Mining (효과적 이모션마이닝을 위한 속성선택 방법에 관한 연구)

  • Eo, Kyun Sun;Lee, Kun Chang
    • Journal of Digital Convergence
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    • v.17 no.3
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    • pp.107-117
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    • 2019
  • In the era of SNS, many people relies on it to express their emotions about various kinds of products and services. Therefore, for the companies eagerly seeking to investigate how their products and services are perceived in the market, emotion mining tasks using dataset from SNSs become important much more than ever. Basically, emotion mining is a branch of sentiment analysis which is based on BOW (bag-of-words) and TF-IDF. However, there are few studies on the emotion mining which adopt feature selection (FS) methods to look for optimal set of features ensuring better results. In this sense, this study aims to propose FS methods to conduct emotion mining tasks more effectively with better outcomes. This study uses Twitter and SemEval2007 dataset for the sake of emotion mining experiments. We applied three FS methods such as CFS (Correlation based FS), IG (Information Gain), and ReliefF. Emotion mining results were obtained from applying the selected features to nine classifiers. When applying DT (decision tree) to Tweet dataset, accuracy increases with CFS, IG, and ReliefF methods. When applying LR (logistic regression) to SemEval2007 dataset, accuracy increases with ReliefF method.

Synthesis of barium-doped PVC/Bi2WO6 composites for X-ray radiation shielding

  • Gholamzadeh, Leila;Sharghi, Hamed;Aminian, Mohsen Khajeh
    • Nuclear Engineering and Technology
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    • v.54 no.1
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    • pp.318-325
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    • 2022
  • In this study, composites containing undoped and barium-doped Bi2WO6:Ba2+were investigated for their shielding against diagnostic X-ray. At first, Bi2WO6 and barium-doped Bi2WO6 were synthesized with different weight percentages of barium oxide through a hydrothermal process. The as-synthesized nanostructures were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS) and Raman spectroscopy (RS). After that, some shields were generated with undoped and barium-doped Bi2WO6:Ba2+ nanostructure particles incorporated into polyvinyl chloride (PVC) polymer with different thicknesses and 15% weight of the nanostructure. Finally, the prepared samples were exposed to an X-ray tube at 40, 80, and 120 kV voltages, 10 mAs and, 44.5 cm SID (i.e. the distance from the X-ray beam source to the specimen). Linear and mass attenuation coefficients were also calculated for different samples. The results indicated that, among the samples, the one with 7.5 mmol barium-doped Bi2WO6 had the most attenuation at the voltage of 40kV, and the attenuation coefficients would increase with an increase in the amount of barium. The samples with 15 and 17.5 mmol barium-doped Bi2WO6 had higher attenuation than the others at 80 and 120 kV. Moreover, the half-value layer (HVL), tenth-value layer (TVL) and 0.25 mm lead equivalent thickness were calculated for all the samples. The lowest HVL value was for the sample with 7.5 mmol barium-doped Bi2WO6. As the result clearly show, an increment in the barium-doping content leads to a decrease in both HVL and TVL. In every three voltages, 0.25 mm lead equivalent thickness of the barium-doped composites (7.5 mmol and 15 mmol) had less than the other composites. The lowest value of 0.25 mm lead equivalent thickness was 7.5 barium-doped in 40 kV voltage and 15 mmol barium-doped in 80 kV and 120 kV voltages. These results were obtained only for 15% weight of the nanostructure.

Development of a Grid-based Daily Watershed Runoff Model and the Evaluation of Its Applicability (분포형 유역 일유출 모형의 개발 및 적용성 검토)

  • Hong, Woo-Yong;Park, Geun-Ae;Jeong, In-Kyun;Kim, Seong-Joon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.5B
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    • pp.459-469
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    • 2010
  • This study is to develop a grid-based daily runoff model considering seasonal vegetation canopy condition. The model simulates the temporal and spatial variation of runoff components (surface, interflow, and baseflow), evapotranspiration (ET) and soil moisture contents of each grid element. The model is composed of three main modules of runoff, ET, and soil moisture. The total runoff was simulated by using soil water storage capacity of the day, and was allocated by introducing recession curves of each runoff component. The ET was calculated by Penman-Monteith method considering MODIS leaf area index (LAI). The daily soil moisture was routed by soil water balance equation. The model was evaluated for 930 $km^2$ Yongdam watershed. The model uses 1 km spatial data on landuse, soil, boundary, MODIS LAI. The daily weather data was built using IDW method (2000-2008). Model calibration was carried out to compare with the observed streamflow at the watershed outlet. The Nash-Sutcliffe model efficiency was 0.78~0.93. The watershed soil moisture was sensitive to precipitation and soil texture, consequently affected the streamflow, and the evapotranspiration responded to landuse type.

Performance and Meat Quality of Three-Crossbreed Korean Native Chickens (KNC) (한국토종닭 3원교배종의 생산성과 육질 특성)

  • Park, Mi-Na;Hong, Eui-Chul;Kang, Bo-Seok;HwangBo, Jong;Kim, Hak-Kyu
    • Korean Journal of Poultry Science
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    • v.38 no.4
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    • pp.293-304
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    • 2011
  • The current work was carried out to investigate the effect of crossbred Korean native chickens (KNC) on performance and meat quality. A total of 720 chicks (1d of age) was used in this work and were divided into groups by crossbreds (A, B, C and D) and sex (male and female). Crossbreds were A) (KNC egg-meat type C strains ${\times}$ KNC meat type S strains) ${\times}$ Ross broiler, B) (KNC egg-meat type C strains ${\times}$ KNC meat type H strains) ${\times}$ KNC meat type S strains, C) (KNC native R strains${\times}$KNC meat type S strains)${\times}$KNC meat type H strains and D(White Semibroiler Chickens). Experimental diets consisted of 3 phases such as starter (0~5 weeks; CP 20.0%, ME 3,050 kcal/kg), earlier (5~8 weeks; CP 18.0%, ME 3,100 kcal/kg) and finisher (8~12 weeks; CP 16.0%, ME 3,150 kcal/kg). They were fed the broiler diets for 12 weeks at the flat house and thirty six chickens were slaughtered at week 5 and 10. There was no significant difference on the fertility of crossbred KNCs, and the hatchability of B crossbred was low compared to other crossbreds. On body weight (BW), D crossbred resulted in a higher BW after 5 weeks (P<0.05). Body weight gain (BWG) and feed intake (FI) of A crossbred were also significant higher compared to the other crossbreds for all periods. On carcass ratio (CR), A crossbred showed higher CR at 5 weeks, there was no significant among crossbreds at 10 weeks. The partial meat ratio(ala, breast, neck, leg) of C crossbred was the lowest among other crossbreds (P<0.05), but the back meat ratio was not difference among treatments at 5 and 10 weeks. pH of meats have no difference among crossbreds at 5 week, and A crossbred was high compared to other crossbreds at 10 week (P<0.05). The moisture content of D crossbred meat and the protein content of B crossbred meat were high compared to other crossbreds at 5 week. Chemical compositions of meats have no difference among crossbreds at 10 week. Lightness and redness have not significance among crossbreds at 5 week, and redness of A crossbred was the highest among all crossbreds (P<0.05). Shear force and cooking loss of A crossbred were high at 5 week, and cooking loss of A crossbred was low compared with other crossbreds at 10 week. These results suggested the basic data that needed to develope the new strains.

Evaluation of Extruded Rice Product as Alternative to Lactose on Growth Performance, Diarrhea Score, Fecal E.coli and VFA in Weaning Pigs (유당 대체제로서 쌀 가공품의 수준별 첨가가 이유자돈의 성장 능력, 설사빈도 및 분내 대장균과 휘발성 지방산에 미치는 영향)

  • Kim, Kwang-Ho;Choi, Young-Hwan;Long, Hong-Feng;Ju, Won-Seok;Son, Seong-Hoon;Oh, Hee-Kyung;Kim, Yoo-Yong
    • Journal of Animal Science and Technology
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    • v.50 no.6
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    • pp.819-830
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    • 2008
  • The object of this study was to investigate different levels of extruded rice product(Nutri-RS®) supplementation as an alternative to lactose on growth performance, diarrhea score, fecal E.coli and volatile fatty acid(VFA) in weaning pigs. Treatments were 1) Control(basal), L3(basal+3% extruded rice product), L6(basal+6% extruded rice product). The control diet in Phase I and Phase II contained 16 and 9% of lactose, respectively. For the feeding trial, a total of 81 crossbred pigs, averaging 7.56±1.18 kg body weight, were allotted to three treatments in three replicates with nine pigs per pen by RCBD. The experimental period was total 5 weeks. During the whole experimental period, there were no significant differences in growth performance, diarrhea score, fecal volatile fatty acid concentration among all treatments. Although there were no significant differences, pigs fed diet of L6 tended to show higher BW and ADG, ADFI, and fecal volatile fatty acid concentration compared to other treatments. Treatment L6 showed the lowest fecal E. coli count among all treatments(P<0.05). These results suggested that inclusion of extruded rice product has similar performance a with 6% of lactose in diet for weaning pigs and reduce approximately 9% production cost of weaning pig's diet. Therefore, extruded rice product could be utilized as an alternative feed ingredient to lactose in weaning pig’s diet.

Analysis of the Effect of Heat Island on the Administrative District Unit in Seoul Using LANDSAT Image (LANDSAT영상을 이용한 서울시 행정구역 단위의 열섬효과 분석)

  • Lee, Kyung Il;Ryu, Jieun;Jeon, Seong Woo;Jung, Hui Cheul;Kang, Jin Young
    • Korean Journal of Remote Sensing
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    • v.33 no.5_3
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    • pp.821-834
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    • 2017
  • The increase in the rate of industrialization due to urbanization has caused the Urban Heat Island phenomenon where the temperature of the city is higher than the surrounding area, and its intensity is increasing with climate change. Among the cities where heat island phenomenon occurs, Seoul city has different degree of urbanization, green area ratio, energy consumption, and population density in each administrative district, and as a result, the strength of heat island is also different. So It is necessary to analyze the difference of Urban Heat Island Intensity by administrative district and the cause. In this study, the UHI intensity of the administrative gu and the administrative dong were extracted from the Seoul metropolitan area and the differences among the administrative districts were examined. and linear regression analysis were conducted with The variables included in the three categories(weather condition, anthropogenic heat generation, and land use characteristics) to investigate the cause of the difference in heat UHI intensity in each administrative district. As a result of analysis, UHI Intensity was found to be different according to the characteristics of administrative gu, administrative dong, and surrounding environment. The difference in administrative dong was larger than gu unit, and the UHI Intensity of gu and the UHI Intensity distribution of dongs belonging to the gu were also different. Linear regression analysis showed that there was a difference in heat island development intensity according to the average wind speed, development degree, Soil Adjusted Vegetation Index (SAVI), Normalized Difference Built-up Index (NDBI) value. Among them, the SAVI and NDBI showed a difference in value up to the dong unit and The creation of a wind route environment for the mitigation of the heat island phenomenon is necessary for the administrative dong unit level. Therefore, it is considered that projects for mitigating heat island phenomenon such as land cover improvement plan, wind route improvement plan, and green wall surface plan for development area need to consider administrative dongs belonging to the gu rather than just considering the difference of administrative gu units. The results of this study are expected to provide the directions for urban thermal environment design and policy development in the future by deriving the necessity of analysis unit and the factors to be considered for the administrative city unit to mitigate the urban heat island phenomenon.

Antioxidant Activities of Stachys sieboldii MIQ Roots (초석잠(Stachys sieboldii MIQ) 뿌리 추출물의 항산화 활성 고찰)

  • 백홍석;나영수;김도한;이창한;류병호;송승구
    • Journal of Life Science
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    • v.14 no.1
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    • pp.1-7
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    • 2004
  • The root of Stnchys Sieboldif MIQ was extracted three times with methanol and extract was found to contain 3.02% of polyphenols and 1.97% of flavonoids. DPPH radical scavenging method, ferric thiocyanate method, and nitrite scavenging ability method were employed to investigate the constituents of the extract and to measure their activity on antioxidation. The fraction extracted by ethylacetate showed higher anti oxidation value than that of $\alpha-tocopherol$, butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT) at the same concentration. UV-VIS spectral data of the extract by ethylacetate that was isolated on a silica gel column proved adsorption maxima in the range of 280∼330 nm. The fraction ES-RS that has $\lambda_{max}(nm)$ of band 1, 325nm and band II, 289nm exhibitd the strongest activity on antioxidation. ES-R5 fraction showed similar pattern to flavones by the analysis of UV-VIS spectral data.