• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.434-437
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• 2001

배양 조건으로 온도는 4, 11, 18, 24, $32^{\circ}C$로 배양하여, $24^{\circ}C$에서 Lipid 내에 약 42.3%의 DHA 최대 수율을 보였으며, 교반속도는 50, 100, 150, 200rpm 으로 실험하여 l00rpm에서 최대수율로 42.1% 올 얻었다. 초기 당농도는 5, 8, 11, 14, 17, 23, 29, 35g/L로 주어 8g/L에서 Lipid 내 DHA 농도가 43.1%로 나타났으며, N/C 비율은 glucose 5 g/L로 고정하여0.1, 0.2, 0.4, 0.6, 0.8 에서 38-40 % 로 거의 일정하게 나타났다.

• 한국수산과학회지
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• 제47권1호
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• pp.31-38
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• 2014

Docosahexaenoic acid (DHA, 22:6n-3) and ecosapentaenoic acid (20:5n-3) have attracted increasing attention since the first epidemiological report on the importance of n-3 essential fatty acids. It is thought that DHA has important functions in brain and retinal tissues. Thraustochytrids, a group of marine protists, are capable of heterotrophic growth, and are potential omega-3 producers for industrial use, especially the members of the Schizochytrium and Thraustochytrium genera. The aims of this work were to isolate, identify and screen thraustochytrids from 17 different locations. Twenty-three isolates were screened for biomass, total fatty acid (TFA) and DHA content. Analysis of the fatty acid methyl esters revealed four distinct clusters biomass ranged from $8.68-9.36gL^{-1}$, and lipid and DHA contents ranged from $3.11-4.10gL^{-1}$ and $1.05-1.93gL^{-1}$ biomass, respectively. B-12 isolates were screened for biomass ($9.36gL^{-1}$), TFA ($4.10gL^{-1}$) and DHA (47.01%, w/w) content. C-6 isolates were also screened for biomass ($8.92gL^{-1}$), TFA ($3.30gL^{-1}$) and DHA (49.41%, w/w) content. The 18S rRNA gene sequencing results identified Schizochytrium mangrovei as B-12 and Crypthecodium cohnii as C-6.