• Molecules and Cells
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• v.45 no.5
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• pp.329-342
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• 2022

The liver is the predominant metastatic site for pancreatic cancer. However, the factors that determine the liver metastasis and the specific molecular mechanisms are still unclear. In this study, we used human pancreatic cancer cell line Hs766T to establish Hs766T-L3, a subline of Hs766T with stable liver metastatic ability. We performed RNA sequencing of Hs766T-L3 and its parental cell line Hs766T, and revealed huge differences in gene expression patterns and pathway activation between these two cell lines. We correlated the difference in pathway activation with the expression of the four core transcriptional factors including STAT1, NR2F2, GATA2, and SMAD4. Using the TCGA database, we examined the relative expression of these transcription factors (TFs) in pan-cancer and their relationship with the prognosis of the pancreatic cancer. Among these TFs, we considered GATA2 is closely involved in tumor metastasis and may serve as a potential metastatic driver. Further in vitro and in vivo experiments confirmed that GATA2-mediated transcriptional activation of Notch3 promotes the liver metastasis of Hs766T-L3, and knockdown of either GATA2 or Notch3 reduces the metastatic ability of Hs766T-L3. Therefore, we claim that GATA2 may serve as a metastatic driver of pancreatic cancer and a potential therapeutic target to treat liver metastasis of pancreatic cancer.

• The Journal of Korean Academy of Prosthodontics
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• v.34 no.2
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• pp.290-298
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• 1996

The effects of impression tray disinfection procedures on the bond strength of impression-material adhesive to two of types resin trays were evaluated with a tensile test. Autopolyme-rizing acrylic resin was formed into 1x1x1 cm cubes. A hook was attached to each cube with autopolymerizing acrylic resin. Perforated trays were fabricated with stops to maintain an even 3 cm of impression material over the resin block. Hook on the opposite side of the perforated tray permitted attachment of the metal plate to a mechanical testing machine. Before adhesive was applied, one third of the resin specimens were immersed in a 5% sodium hypochlorite solution : one third in a 2% glutaradehyde solution, and one third were kept in the "as fabricated" condition. Three products(Perfect ups, Exafine, and Exp-ress) of polyvynil siloxane impression material-adhesive system were evaluated. The resin-impression material-metal plate couples were attached to a mechanical testing machine and tensile forces were applied at a separation rate of 10 centimeters per second. The results were as follows; 1. Both disinfectant and adhesive had effects on bond strength values, but adhesive had more effect than disinfectant(p<0.01). However, there was no interaction between dinin-fectant and adhesive(p>0.01). 2. Mean bond strength values for the Perfect materials were about 85% less than that of the Exafine or the Express materials. However, there was no stastically significant difference between the mean bond strength values of the Exafine materials and the Express materials(p>0.05). 3. The use of disinfectants produced significant reduction in bond strength values(p<0.05). But there was no stastically significant difference between the mean bond strength values of the groups treated with disinfectants(p>0.05).

• Journal of Oral Medicine and Pain
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• v.20 no.2
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• pp.385-397
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• 1995

The purpose of this study is to document the stress symptoms (Anxiety and depression) among dental students and the difference of stress level between classes and by gender. All students in dental college, Seoul National University, in the 1995 academic year, were tested with the Spielberger's State-Trait Anxiety Inventory and Beck Depression Inventory just before the first semestral examination when the stress levels of students are expected to be the highest. 303 students answered the test. The obtained results were as follows : 1. State Anxiety inventory scores of the second-year and first-year students were significantly higher than those of the fourth -year and third-year(p(0.01), and all classes showed mean state anxiety scores more than mild. 2. Trait Anxiety inventory scores of the second-year and first-year students were significantly higher than those of the third-year and forth-year(p(0.01), and all classes showed mean Trait Anxiety scores more than mild. 3. Beck Depression inventory scores of the second -year students were significantly higher than those of the third-year and fourth-year(p(0.01), and twenty two students(7.3%) among the fourth classes showed depression symptoms. 4. In the comparison of the mean scores of the State-Trait Anxiety Inventory and Beck Depression Inventory between male and female students, State Anxiety Inventory scores of the second-year female students were significantly higher than those of the second year male students(p(0.05), and there was no significant difference elsewhere.

• Journal of Dental Rehabilitation and Applied Science
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• v.23 no.3
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• pp.239-248
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• 2007

The accuracy and dimensional stability of rubber impression materials are very important for the fitness of the final restoration. The purpose of this investigation was to evaluate the effects of the storage time and pouring frequency of the additional silicone materials on dimensional stability. Total 30 impressions were made of tooth prepared single crown. The dies had 1 buccolingual, 1 mesiodistal and 1 occlusogingival lines. Two additional silicone materials(examixfine, aquasil) were included. 15 specimens were made of each impression material and poured by type IV stone over times(1hour, 24hours, 72hours) after mixing. 5 impressions poured after 1 hour were used for second pouring after 24hours and third pouring after 72hours. The same examiner measured each specimen 3 times after lazer scanning. All statistical tests were performed with the level of significance set at 0.05. The results indicated that significant difference at any measuring point of stone dies except the buccolingual and mesiodistal lines of the specimen made at 1hour after mixing with examixfine when measurements at 1hour, 24hours and 72hours were campared. There were significant difference between two impression materials and at every measuring point of stone dies made at second and third pouring. The length of measuring point increased significantly as time passed by and increase of the pouring frequency. However, this results are clinically acceptable except the dies of the third pouring. Under the conditions of this study, the shrinkage rate of the additional silicone rubber impression materials significantly increased as time passed by and increase of the pouring frequency.

• The Journal of the Korean dental association
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• v.58 no.5
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• pp.276-283
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• 2020

Objectives: The aim of this study was to evaluate the efficacy of CK files as an ultrasonic instrument, and to determine most efficient file size for smear layer removal. Materials and Methods: Thirty-six extracted human mandibular premolars with single, straight root canals and mature apices were mechanically prepared and randomly divided into three groups. Group 1 (Control) underwent conventional needle irrigation, Group 2 (CKS) underwent passive ultrasonic irrigation with a #20 CK file, and Group 3 (CKL) underwent passive ultrasonic irrigation with a #30 CK file. After preparation and irrigation, all teeth were dried and split with a chisel to obtain the mesial and distal half of their roots. Each sample was evaluated using a scanning electron microscope, and data were analyzed using the Kruskal-Wallis and Mann-Whitney rank sum tests (p<0.05). Results: The CKS group showed less debris in the apical third than the other groups (p<0.05). In this section, no significant difference was observed among the other groups. And, there was no significant difference among any groups for the middle third section. Conclusion: This study showed that PUI with #20 CK file removed more smear layer compared to using #30 CK file at the apical third of the root canal.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.46 no.4
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• pp.250-257
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• 2020

Objectives: Palatal infiltration is the most painful and uncomfortable anesthesia technique for maxillary impacted third molar surgery (MITMS). This approach could cause patients distress and aversion to dental treatment. The aim of this study was to evaluate the anesthetic efficacy of a buccal infiltration injection without a palatal injection in MITMS. Materials and Methods: This prospective research study was a crossover split mouth-randomized controlled trial. Twenty-eight healthy symmetrical bilateral MITMS patients (mean age, 23 years) were randomly assigned to two groups. Buccal infiltration injections without palatal injections were designated as the study group and the buccal with palatal infiltration cases were the control group, using 4% articaine and 1:100,000 epinephrine. The operation started after 10 minutes of infiltration. Pain assessment was done using a visual analogue scale and a numeric rating scale after each injection and extraction procedure. Similarly, the success rate, hemodynamic parameters, and additional requested local anesthetic were assessed. Results: The results showed that the pain associated with local anesthetic injections between both groups were significantly different. However, the success rates between the groups were not significantly different. Postoperative pain was not significant between both groups and a few patients requested an additional local anesthetic, but the results were not statistically significant. For hemodynamic parameters, there was a significant difference in systolic pressure during incision, bone removal, and tooth elevation. In comparison, during the incision stage there was a significant difference in diastolic pressure; however, other steps in the intervention were not significantly different between groups. Conclusion: We concluded that buccal infiltration injection without palatal injection can be an alternative technique instead of the conventional injection for MITMS.

• Restorative Dentistry and Endodontics
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• v.40 no.2
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• pp.149-154
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• 2015

Objectives: This study evaluated the penetration depth of 2% chlorhexidine digluconate (CHX) into root dentinal tubules and the influence of passive ultrasonic irrigation (PUI) using a confocal laser scanning microscope (CLSM). Materials and Methods: Twenty freshly extracted anterior teeth were decoronated and instrumented using Mtwo rotary files up to size 40, 4% taper. The samples were randomly divided into two groups (n = 10), that is, conventional syringe irrigation (CSI) and PUI. CHX was mixed with Rhodamine B dye and was used as the final irrigant. The teeth were sectioned at coronal, middle and apical levels and viewed under CLSM to record the penetration depth of CHX. The data were statistically analyzed using Kruskal-Wallis and Mann-Whitney U tests. Results: The mean penetration depths of 2% CHX in coronal, middle and apical thirds were $138{\mu}m$, $80{\mu}m$ and $44{\mu}m$ in CSI group, respectively, whereas the mean penetration depths were $209{\mu}m$, $138{\mu}m$ and $72{\mu}m$ respectively in PUI group. Statistically significant difference was present between CSI group and PUI group at all three levels (p < 0.01 for coronal third and p < 0.001 for middle and apical thirds). On intragroup analysis, both groups showed statistically significant difference among three levels (p < 0.001). Conclusions: Penetration depth of 2% CHX into root dentinal tubules is deeper in coronal third when compared to middle and apical third. PUI aided in deeper penetration of 2% CHX into dentinal tubules when compared to conventional syringe irrigation at all three levels.

• Korea Journal of Hospital Management
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• v.12 no.3
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• pp.47-67
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• 2007

This paper aims at suggesting several ways lo change financial vulnerability and to improve managerial capability of local public hospitals (LPHs) in Korea through the identification of factors affecting profitability. Several findings of the research are as follows: To begin with, LPHs exhibited a statistically significant difference in their profitability from one another, according to tile analyses of their profitable margins from tile general characteristics. It depends on the number of hospitals in the area, the population of the hospital-built area, the number of competing hospitals, the number of staff per 100 beds, the opening of special clinic, the educational function, and the capacity of rooms. However, there was no variable in the managerial characteristics, presenting a significant difference, in contrast with hospitals which have been managed by private companies and made a great amount of profits. Second, according to the analyses of profit differences in behavioral effort-characteristics, a statistically significant difference was revealed upon the basis of the efforts to improve the clinic service, invite special patients, and shorten the period of being hospitalized. Third, the result of analyses about the difference of profitability from medical care and finance is statistically significant in the rate of labor cost, the rate of management cost, bed-occupancy rate, and the period of being hospitalized. Fourth, according to the analyses of the factors influencing the net profit ratio of the entire capital, Adjusted explanatory power(Adjusted $R^2$) was shown up to 65.2%, which is high. To compare the adjusted explanatory power stage by stage, the first stage model applying only two variables such as structural and strategic characteristics exhibited 23.8%, and the second stage model adding financial characteristics showed 51.5%. The explanatory power was much improved up to 65.2% when the third stage model incorporated the outcome of medical care performance. When the return on investment(ROI) was examined by using the multi-variate linear regression analysis at the final model of third stage, it was found that ROI had a positive relationship with the increase rate of patients, labor costs per doctor, and medical care rate of socially protected inpatients. However, it revealed that ROI had a negative relationship with the ratio of labor costs, the number of patients per managerial staff, and occupancy rate of rooms, respectively. The research suggests that in order for LPHs to increase profitability, LPH, should make efforts not only to attract patients to the hospitals without any discrimination of the patients depending on their financial status, but also to develop efficient management methods to reduce labor costs.

• Journal of Korean Society for Quality Management
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• v.29 no.3
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• pp.109-123
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• 2001

The purpose of this study is to analyze the differences in the deluxe hotels'service quality in Korea according to the region and the brand. The subjects of this survey for the empirical analysis are limited to the customers of deluxe hotels in Korea: data(1,054 samples) are collected from 16 deluxe hotels among 27 of its kind in Seoul, Pusan, Cheju and Kyongju areas. The results are as follows. First, the hotel service quality between the hotels in Seoul and in provinces makes little difference. Second, the service quality in the Seoul, Pusan, Cheju and Kyongju areas shows significant difference. Third, there is also a significant difference on the service quality between domestic hotel brand and foreign-chained hotel brand.

• 한국전산유체공학회:학술대회논문집
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• 2006.10a
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• pp.22-25
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• 2006

Direct simulations of aerodynamic sound, especially sound emitted by rapidly rotating elliptic cylinder by the finite difference lattice Boltzmann method (FDLBM). Effect of pile-fabrics for noise reduction is also studied by the finite volume LBM (FVLBM) using an unstructured grid. Second order time integration and third order upwind scheme are shown to be enough for these simulations. Sound sources are detected to be doublets for both cases. For the elliptic cylinder, the doublet is generated in the interaction between the vortex and the edge. For the circular cylinders, they are generated synchronizing with the Karman vortex street, and it is also shown that the pile-fabrics covering the surface of the cylinder reduces the strength of the source.