• Title/Summary/Keyword: Thiosulfate oxidizing bacteria

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Ubiquitous Presence and Activity of Thiosulfate Oxidizing Bacteria in Rhizosphere of Economically Important Crop Plants of Korea (국내 작물 근권에 서식하는 황산화세균의 분포와 합성)

  • Yim, Woo-Jong;Anandham, R.;Gandhi, P. Indira;Hong, In-Soo;Islam, M.R.;Trivedi, P.;Madhaiyan, M.;Han, Gwang-Hyun;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.1
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    • pp.9-17
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    • 2008
  • The presence of thiosulfate oxidizing bacteria was examined in rhizosphere soils of 19 economically important plant species belonging to 10 different families. The results showed that the thiosulfate oxidizing bacteria were present in all the tested rhizosphere soils, and the total 32 thiosulfate oxidizing bacteria were recovered. Furthermore, the biochemical characterization revealed that 56% and 44% of the isolates belonged to the obligate chemolithoautotrophs and facultative heterotrophs, respectively. The isolates ATSR15P utilized 19.17 mM of thiosulfate and accumulated 11.65 mM of sulfate in the medium. Concurrently, the decrease in pH of the medium was observed. This study comprehensively demonstrates that the active sulfur oxidation is a ubiquitous phenomenon in the rhizosphere of crop plants in Korea.

Isolation and Characterization of Sulfur-oxidizing Denitrifying Bacteria Utilizing Thiosulfate as an Electron Donor (황(thiosulfate)을 이용하는 탈질 미생물의 분리 및 특성 파악)

  • Oh, Sang-Eun;Joo, Jin-Ho;Yang, Jae E
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.6
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    • pp.409-414
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    • 2006
  • Sulfur-oxidizing bacteria were enumerated and isolated from a steady-state anaerobic master culture reactor (MCR) operated for over six months under a semi-continuous mode and nitrate-limiting conditions using thiosulfate as an electron donor. Most are Gram-negative bacteria, which have sizes up to 12 m. Strains AD1 and AD2 were isolated from the plate count agar (PCA), and strains BD1 and BD2 from the solid thiosulfate/nitrate medium. Based on the morphological, physiological, FAME and 16S rDNA sequence analyses, the two dominant strains, AD1 and AD2, were identified as Paracoccus denitrificans and Paracoccus versutus (formerly Thiobacillus versutus), respectively. From the physiological results, glucose was assimilated by both strains AD1 and AD2. Heterotrophic growth of strains AD1 and AD2 could be a more efficient way of obtaining a greater amount of biomass for use as an inoculum. Even though facultative autotrophic bacteria grow under heterotrophic conditions, autotrophic denitrification would not be reduced.

Optimization of Mutant Strain of the Sulfur-Oxidizing Bacteria, Thiobacillus sp. UIW-6 (황산화 세균 Thiobacillus sp. UIW-6 변이주의 성장 최적화)

  • Shin, Seung-Yong;Kang, Sun-Chul
    • Korean Journal of Environmental Agriculture
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    • v.25 no.2
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    • pp.124-128
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    • 2006
  • To reducing offensive odor form compost UIW-6 mutant obtained by UV treatment from sulfur-oxidizing bacteria, Thiobacillus sp. IW. The UIW-6 mutant was found 1.6 times faster in growth than the parent strain on thiosulfate medium (TM) at 36 h after incubation. Initial pH, temperature and agitation for the optimum growth of UIW-6 were 6.5, $35^{\circ}C$ and 200 rpm, respectively. The UIW-6 mutant growth was two times higher than parent strain at 6 h culture in TM liquid medium containing 50 mM sodium thiosulfate. The UIW-6 mutant used fructose and sucrose as carbon sources and yeast extract> tryptone> peptone as nitrogen ones. It was found that the growth of UIW-6 was increased in addition of 0.2% yeast extract.

Optimization of the Sulfur-oxidzing Bacteria, Thiobacillus novellus SRM (황 산화 세균인 Thiobacillus novellus SRM 성장 최적화)

  • 권규혁;차월석;고한철;이광연;박돈희;차진명
    • KSBB Journal
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    • v.18 no.6
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    • pp.443-447
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    • 2003
  • The microorganism was isolated from the night soil treatment plant for the removal of sulfur compounds. The growth conditions of the sulfur-oxidizing bacteria were investigated and the isolate characterized as Thiobacillus noveilus SRM. The optimal pH of Thiobacillus novellus SRM on cell growth was pH 7.0 and the optimal temperature was 30$^{\circ}C$ and the optimal air flow rate was 1 vvm, respectively. As a results of cell growth from the Monod plot, the specific growth rate was 0.032 hr$\^$-l/, $V_{max}$ was 1.43 hr$\^$-l/ and $K_{m}$ was 0.32, respectively. The thiosulfate oxidation by Thiobacillus novellus SRM was made of sulfate ion. The sulfate ion reduced pH and decreased cell growth.

Isolation of Sulfur Oxidizing Bacteria from Different Ecological Niches (다양한 생태계에서 분리한 황 산화 세균)

  • Anandham, Rangasamy;Sridar, Ragupathy;Nalayini, Periyakaruppan;Madhaiyan, Munusamy;Gandhi, Pandiyan Indira;Choi, Kwan-Ho;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.38 no.4
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    • pp.180-187
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    • 2005
  • Nine chemolithoautotrophic and 12 chemolithoheterotrophic sulfur oxidizing bacteria were isolated using enrichment technique in modified Starkey's medium. All isolates reduced pH of the growth medium through oxidation of elementai sulfur to sulfuric acid. Isolates utilized the thiosulfate as energy source except LCH. None of the isolates grew anaerobically and utilization of glucose was found only in chemolithoheterotrophic isolates SGA6 and JIG. In vitro sulfate production from elemental sulfur was found maximum for chemoiithoautotroph LCH ($43.2mg\;100\;mL^{-1}$) and least for chemolithoheterotroph JIG ($10.04mg\;100\;mL^{-1}$). The above tests suggested that all isolates belong to the member of Thiobacillus. For field inoculation of Thiobacillus, clay based pellet formulation was developed with the cell load of $2.5{\times}10^7cfu\;g^{-1}$ of pellet. It is easy to handle by the farmers and more likely to lead to successful farming.

Development and culture optimization of mutants of Thiobacillus sp. IW for elimination of hydrogen sulfide

  • Kang, Sun-Chul;Lim, Kwang-Hee;Shin, Seung-Yong
    • 한국생물공학회:학술대회논문집
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    • 2005.10a
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    • pp.465-467
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    • 2005
  • UIW-10 mutant obtained by UV treatment using sulfur-oxidizing bacteria, Thiobacillus sp. IW was studied. The colony size of UIW-10 was found 2 $^{\sim}$ 3 times bigger in diameter than the parent colony on TAM medium. UIW-10 mutant growth was two times higher than parent strain at 6 h culture in liquid medium containing sulfides such as sulfur and sodium thiosulfate. Initial pH and temperature for the optimum growth of UIW-10 were 6.0 and $35-40^{\circ}C$, respectively. It was found that addition of 0.5% yeast extract and 0.5 to 2.0% tryptone as nitrogen sources and the constant agitation at 150 to 200 rpm had a positive effect and the growth of UIW-10 was increased.

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