• Journal of Integrative Natural Science
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• v.8 no.4
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• pp.267-272
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• 2015

Galactosemia is a potentially lethal disorder caused by the deficiency of the enzyme galactose-1-phosphate uridyltransferase (GALT) within the Leloir pathway. Galactokinase (GALK) is the enzyme in Leloir pathway which converts ${\alpha}$-D galactose to galactose 1-phosphate. The elevated level of galactose-1-phosphate, the product of GALK plays a major role in Galactosemia. Therefore the inhibition of GALK is a novel therapy for this disorder. Hence in the present study, we performed molecular docking of twenty inhibitors with different activity against galactokinase into the active site of galactokinase enzyme. The binding mode of these inhibitors was obtained using Surflex dock program interfaced in Sybyl-X2.0. The residues such as SER141, TYR109, ARG105, ARG228, TYR106, GLY346, GLY136, ASP86, ASP186 and SER142 found to interact with inhibitors.

• Natural Product Sciences
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• v.23 no.4
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• pp.227-234
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• 2017

Moringa oleifera Lam (M. oleifera, Moringaceae) is a tree of the Moringaceae family that can reach a height of between 5 and 10 m. The current paper presents cytotoxic effect of M. oleifera fruits and its flavonoids 1 and 2. The viability of HCT116 human colon cancer cells were 38.5% reduced by $150{\mu}g/mL$ of ethanolic extracts in a concentration-dependent manner; in addition, we observed the apoptotic features of cell shrinkage and decreased cell size. Bcl-2 family proteins were regulated as determined by Western blotting analysis, suggesting that M. oleifera fruits and their flavonoids 1 and 2 induced apoptosis through an intrinsic pathway. Based on our findings, 70% ethanolic extracts of M. oleifera fruits and flavonoids 1 and 2 might be useful as cytotoxic agents in colorectal cancer therapy.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.6
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• pp.550-552
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• 2006

Periosteum-derived progenitor cells (PDPCs) were isolated using a fluorescence-activated cell sorter and their chondrogenic potential in biomaterials was investigated for the treatment of defective articular cartilage as a cell therapy. The chondrogenesis of PDPCs was conducted in a thermoreversible gelation polymer (TGP), which is a block copolymer composed of temperature-responsive polymer blocks such as poly(N-isopropylacrylamide) and of hydrophilic polymer blocks such as polyethylene oxide, and a defined medium that contained transforming growth $factor-{\beta}3\;(TGF-{\beta}3)$. The PDPCs exhibited chondrogenic potential when cultured in TGP. As the PDPCs-TGP is an acceptable biocompatible complex appropriate for injection into humans, this product might be readily applied to minimize invasion in a defected knee.

• Natural Product Sciences
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• v.14 no.4
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• pp.244-248
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• 2008

It is well known that excessive production of reactive oxygen species (ROS) leads to oxidative stress, loss of cell function, and ultimately apoptosis or necrosis. To search for natural antioxidants able to modulate cellular oxidative stress, we investigated the protective effect of ethanol extracts of 17 medicinal plants selected from the preliminary antioxidant screening on tert-butyl hydroperoxide (t-BuOOH)-induced oxidative stress in human keratinocytes. The result showed that extracts of the four plants, Distylium racemosum, Astilbe chinensis, Cercis chinensis and Sapium japonicum, exhibited significant cytoprotective activity (over 50% protection) against t-BuOOH-induced cellular injury.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.34 no.1
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• pp.44-49
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• 2021

Recently, infrared (IR) and near-infrared (NIR) light-emitting diodes (LEDs) were widely used for home medical applications owing to its low output power and wide exposed area for curing. For deep penetration of the light under the skin, multiple LEDs with wavelengths of 700~10,000 nm were located on a flexible printed circuit board. When multiple wavelengths of LEDs were soldered on a circuit board, the lifetime of LED module highly depends on LEDs with a short lifetime. The mean time to failure (MTTF) was able to calculate with the experimental results under high temperature and the Arrhenius model. The results of this study could help companies to approve the warranty of LED modules and its product.

• Physical Therapy Korea
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• v.8 no.2
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• pp.41-54
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• 2001

Fatigue is the decline in force produced as a result of prolonged muscle activity. Localized muscle fatigue can be identified by a shift toward low in the frequency components of the EMG signal, typically represented by a fall in the median frequency. Previous studies show that a shortened muscle develops a higher fatigue than elongated muscles. The purpose of this study was to investigate the time-related change of median frequency and torque during maximal isometric back extension exercises at different exercise angles ($0^{\circ}$, $12^{\circ}$, $36^{\circ}$, $72^{\circ}$). Twenty healthy subjects (mean age = $24.35{\pm}2.70$) were evaluated in this study. Median frequency was extracted from the EMG signals by fast Fourier transform (FFT). Initial median frequency and the slope of median frequency change over time were computed from linear regression analysis. Pearson's product moment correlation was used to quantify the relationship between slope of median frequency and torque. The results were as follows: 1) Significant differences in y-intercepts of torque regression equation with respect to exercise angle were shown. However, there were no differences in the slopes of the median frequency and torque, and y intercept of the median frequency among exercise angles. 2) There was no significant correlation between slope of median frequency and torque. 3) But there was moderate correlation between median frequency and torque at each exercise angle. In conclusion, the exercise angle during maximal isometric back extension exercise is not a direct effect on slope of median frequency and torque. But results showed that median frequency and torque shift were highly correlated in all subjects.

• Korean Journal of Food Science and Technology
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• v.36 no.5
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• pp.779-783
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• 2004

New food ingredient was developed to eradicate and protect against re-infection of Helicobacter pylori in fermentation broth of lactic acid bacteria (LAB) showing antimicrobial activity against pathogenic microorganisms such as H. pylori and Listeria monocytogenes. LAB strain CBT SL4 was identified as Pediococcus pentosaceus by 16S rDNA sequencing and its culture broth showed antimicrobial activity of 800 AU/mL against H. pylori in optimized fermentation process. Using thin layer concentration system and spray-typed fluid bed drier system, concentrated powder product showing activity of 12,800 AU/g was harvested. Product showed eradication and protection activities against H. pylori infection on feeding test (50 AU/day) using Mongolian gerbil infection model. After 4 weeks therapy of 8,000 AU/day, ${\Delta}13CO_2$ level (DOB30) decreased about 40% in urea breath test on patient with H. pylori infection. Result show concentrated culture product of P. pentosaceus CBT SL4 has eradicating and protecting activities against H. pylori infection and can be used as food-active ingredient for prevention of gastric and duodenum ulcer caused by H. pylori.

• The Journal of Korean Academy of Sensory Integration
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• v.21 no.3
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• pp.27-38
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• 2023

Objective : This study aims to verify the validity and reliability of the Korean version of the Sensory Processing and Self-Regulation Checklist (SPSRC) for children with and without autism spectrum disorder. Methods : The Pearson product-moment correlation coefficient was calculated using Short Sensory Profile (SSP) to verify concurrent validity. Construct validity was verified by comparing the sensory processing ability and self-regulation ability of the two groups. Cronbach's α was calculated in the case of internal consistency for reliability verification, and the test-retest reliability was verified through the Pearson correlation coefficient. Results : Based on the verification of the concurrent validity, the Korean version of SPSRC and SSP showed a statistically significant correlation (p < 0.01). The construct validity was found to have a statistically significant difference between the two groups in the area and sub-items of the Korean version of SPSRC (p < 0.001). For the internal consistency, Cronbach's α ranged from 0.700 to 0.975. The test-retest reliability showed that the correlation coefficient ranged from 0.937 to 0.997. Conclusion : The Korean version of SPSRC was confirmed to be an evaluation tool with high validity and reliability. It is expected to be used as an evaluation tool for planning treatment goals in clinical trials and as a meaningful basis for future research.

• The Journal of Korean Society for Radiation Therapy
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• v.5 no.1
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• pp.142-151
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• 1992

Radioprotective effects of a red ginseng extracts on antioxidant enzymes(Superoxide dismutase, catalase and peroxidase) activities relationship to lipid peroxidation were studied in the cytosol fraction of mice liver. The experiments were carried out on Irradiated (5.5 Gy, $^{\60}Co$) and non-irradiated ICR mice after treatment of red ginseng extracts (5.5mg/mouse ; ip), In wholebody irradiated mice, irradiation caused a decrease in the activity of all these enzymes(on Day 21) The activities of SOD, Catalase and Peroxidase of red ginseng extracts treated mice were enhanced by $35.4\%,\;20.2\%$ and $20.1\%$, compared with non-treated mice. The red ginseng extracts led to inhibited increase of malondialdehyde product by ionizing radiation. The enhanced activity of enzymes that removed free radicals generated by radiation and thereby indicate that ginseng probably plays on important role in radioprotective effect.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.4
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• pp.2599-2605
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• 2013

The study aimed to evaluate immune-stimulating effects of a well-known Thai folkloric remedy when used for adjuvant therapy with conventional chemotherapeutics for treatment of breast cancer. Immunostimulating influence of the remedy (215 mg/kg body weight per day) on NK cell activity and TNF-${\alpha}$ release from the monocytes/macrophages were investigated in a total of 15 healthy women and 13 female patients with breast cancer (Group 1). The effect of breast tumor surgery on NK cell activity was further investigated in 18 female patients with breast cancer (Group 2). NK cell cytotoxic activity was determined by chromium release cytotoxic assay using K562, an erythroleukemic cell line. TNF-${\alpha}$ release from monocytes/macrophages separated from blood samples was determined through a biological assay using actinomycin D-treated L929 mouse fibroblast cells in the presence and absence of LPS. Baseline NK cell activity of the monocytes/macrophages separated from Group 2 patients expressed as %cytotoxicity was significantly lower than in the healthy subjects at E:T ratios of 100:1 and 25:1. In healthy subjects, there was no change in NK cell cytotoxic activity (%cytotoxicity or LU) following 1 and 2 weeks of treatment with the remedy compared with the baseline at various E:T ratios but the binding activity (%binding) was significantly increased after 2 weeks of treatment. The addition of one or two conventional chemotherapeutic regimens did not significantly reduce the NK cytotoxic activity but did affect release of TNF-${\alpha}$ in both unstimulated and LPS-stimulated samples. Surgery produced a significant suppressive effect on NK cell activity. The use of the remedy as an adjunct therapy may improve therapeutic efficacy and safety profiles of conventional chemotherapeutic regimens through stimulation of the immune system in cancer patients.