Browse > Article

Chondrogenic Properties of Human Periosteum-derived Progenitor Cells (PDPCs) Embedded in a Thermoreversible Gelation Polymer (TGP)  

Choi, Yang-Soo (Department of Biological Engineering, Inha University)
Lim, Sang-Min (Department of Biological Engineering, Inha University)
Shin, Hyun-Chong (Department of Biological Engineering, Inha University)
Lee, Chang-Woo (Good Shepherd Hospital)
Kim, Dong-Il (Department of Biological Engineering, Inha University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.6, 2006 , pp. 550-552 More about this Journal
Abstract
Periosteum-derived progenitor cells (PDPCs) were isolated using a fluorescence-activated cell sorter and their chondrogenic potential in biomaterials was investigated for the treatment of defective articular cartilage as a cell therapy. The chondrogenesis of PDPCs was conducted in a thermoreversible gelation polymer (TGP), which is a block copolymer composed of temperature-responsive polymer blocks such as poly(N-isopropylacrylamide) and of hydrophilic polymer blocks such as polyethylene oxide, and a defined medium that contained transforming growth $factor-{\beta}3\;(TGF-{\beta}3)$. The PDPCs exhibited chondrogenic potential when cultured in TGP. As the PDPCs-TGP is an acceptable biocompatible complex appropriate for injection into humans, this product might be readily applied to minimize invasion in a defected knee.
Keywords
chondrogenesis; periosteum-derived cells; thermoreversible gelation polymer;
Citations & Related Records

Times Cited By Web Of Science : 7  (Related Records In Web of Science)
Times Cited By SCOPUS : 5
연도 인용수 순위
1 Fisher, J. P., S. Jo, A. G. Mikos, and A. H. Reddi (2004) Thermoreversible hydrogel scaffolds for articular cartilage engineering. J. Biomed. Mater. Res. A 71: 268-274
2 Nagaya, M., S. Kubota, N. Suzuki, M. Tadokoro, and K. Akashi (2004) Evaluation of thermoreversible gelation polymer for regeneration of focal liver injury. Eur. Surg. Res. 36: 95-103   DOI   ScienceOn
3 O'Driscoll, S. W. and J. S. Fitzsimmons (2001) The role of periosteum in cartilage repair. Clin. Orthop. Relat. Res. 391S: S190-S207
4 Yoshioka, H., M. Mikami, and Y. Mori (1994) A synthetic hydrogel with thermoreversible gelation: I. Preparation and rheologicla properties. Pure Appl. Chem. A31: 113-120
5 Kleinman, H. K., M. L. McGarvey, L. A. Liotta, P. G. Robey, K. Tryggvason, and G. R. Martin (1982) Isolation and characterization of type IV procollagen, laminin, and heparan sulfate proteoglycan from the EHS sarcoma. Biochemistry 21: 6188-6193   DOI   ScienceOn
6 De Bari, C., F. Dell'Accio, and F. P. Luyten (2001) Human periosteum-derived cells maintain phenotypic stability and chondrogenic potential throughout expansion regardless of donor age. Arthritis Rheum. 44: 85-95   DOI   ScienceOn
7 Oh, I. S. and H. G. Kim (2004) Vascular endothelial growth factor upregulates follistatin in human umbilical vein endothelial cells. Biotechnol. Bioprocess Eng. 9: 201-206   DOI   ScienceOn
8 Hishikawa, K., S. Miura, T. Marumo, H. Yoshioka, Y. Mori, T. Takato, and T. Fujita (2004) Gene expression profile of human mesenchymal stem cells during osteogenesis in three-dimensional thermoreversible gelation polymer. Biochem. Biophys. Res. Commun. 317: 1103-1107   DOI   ScienceOn
9 Lu, L., X. Zhu, R. G. Valenzuela, B. L. Currier, and M. J. Yaszemski (2001) Biodegradable polymer scaffolds for cartilage tissue engineering. Clin. Orthop. Relat. Res. 319S: S251-S270
10 Ochi, M., Y. Uchio, K. Kawasaki, S. Wakitani, and J. Iwasa (2002) Transplantation of cartilage-like tissue made by tissue engineering in the treatment of cartilage defects of the knee. J. Bone Joint Surg. Br. 84: 571-57   DOI
11 Lim, S. M., Y. S. Choi, H. C. Shin, C. W. Lee, and D.-I. Kim (2005) Isolation of human periosteum-derived progenitor cells using immunophenotypes for chondrogenesis. Biotechnol. Lett. 27: 607-611   DOI