• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.3
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• pp.178-184
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• 2002

High-throughput screening has become a popular method used to identify new “leads”for potentially therapeutic compounds. Further screening of these lead compounds is typically done with secondary assays which may utilize living, functioning cells as screening tools. A problem (or benefit) with these cell-based assays is that living cells are very sensitive to their environment. We have been interested in the process of stem cell migration and how it relates to the cellular therapy of bone marrow transplantation. In this study we describe a secondary, cell-based assay for screening the effects of various in-vitro conditions on Immature Hematopoietic Cell (IHC) migration. Our results have revealed many subtle factors, such as the cell's adhesive characteristics, or the effect of a culture's growth phase, that need to be accounted for in a screening protocol. Finally, we show that exponentially glowing KG1a cells (a human IHC cell line) were 10 times more motile than those in the lag or stationary phases. These data strongly suggest that KG1a cells secrete a chemokinetic factor during the exponential growth phase of a culture.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.9 no.1
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• pp.228-235
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• 2008

This research requires personal, psychological analysis which reflect korean culture's characteristics and emotions for stage-fright and factors of fear-causing, solution-centered, humanism, recognition-acting by approaching through psychology counsel. It studies former stage, and base on this psychological therapy. It can be suggested that applying various psychological arbitration training program. Also stage fear and fright which factors are not overcome and explained, with them It is necessary to show alternative plan with put physiological analysis together, with this procedure, The goal is to develop systematical and synthetic training course.

• Phonetics and Speech Sciences
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• v.6 no.4
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• pp.189-194
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• 2014

Objective: This study aims to investigate the speech rate, the length of a pause, habitual pitch, and voice intensity of motherese. Subjects and Methods: The research participants comprised 20 mothers (mean age 33 years). Speech data were collected and analyzed using the Real-time Pitch software (KayPENTAX(R)). Results: The average speech rate was 5.33 syllables per second without their infant present and 4.26 syllables per second with their infant present. The average pause length was 1.09 s without their infant present and 1.56 s with their infant present. The average habitual pitch was 199.79 Hz without their infant present and 227.15 Hz with their infant present. The average voice loudness was 61.09 dB without their infant present and 64.49 dB with their infant present. Conclusion: This study presented clinical information for efficiently managing the speech therapy issues of infants and children. This includes proper acoustic and phonological information to recommend to main caregivers.

• International Journal of Internet, Broadcasting and Communication
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• v.11 no.3
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• pp.77-86
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• 2019

The purpose of this study was to reveal how the agility of fingers directly involved in the use of mobile phones, a necessity for modern people, is related to the amount of text usage. To find out, 95 people who agreed to the purpose of this research without any limitation on visual and upper-geometry were selected. The research period was from June 27, 2018 to July 31, 2018. The evaluation tool used in this study was Grooved Pegboard, a standardized evaluation tool that measures hand dexterity, and the general characteristics of examinees such as age, text message amount, and a hand that using for text messages were investigated through interviews. Since text input methods vary depending on mobile phone types, unfamiliar methods of typing mobile phone characters can affect the speed of texting. As a result, there were significant differences in hand dexterity between age and gender. The rate of texting and hand dexterity were statistically significantly faster than those in their 20s and 30s (p<0.05), and in gender, women showed significantly faster texting and hand dexterity than men (p<0.05). However, it was not statistically significant to text usage and to the dexterity of the hand.

• Child Health Nursing Research
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• v.6 no.2
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• pp.144-157
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• 2000

This study was done to understand nonpharmacologic pain management for pediateric patients and nurses' knowledge and attitudes toward it. The aim of this study was that which method did the patient's use according to the nurses' age, and how did they effectively use these methods in their field. The subjects of this study were 77 nurses working in the Pediatric unit in the Kyung Medical Center from September 2 to 15, 1999 using questionnaire form. The results of this study were as follows : 1. We divided the subjects into four groups : Younger than one year old, 1-6 years, 6-12 years, 12-18 years group. In the group younger one year old, most of the nurses participating in this study used speaking in soft quiet tones, supportive touch, toys, pacifiers. In the group of 1-6 years, they used speaking in soft quiet tones, toys, distracting attention, story talking, and visual stimulus. In the group of 6-12 years. they used pop-up books, providing information, cold therapy, speaking in soft quiet tones, supportive touch. In the group of 12-19 years, most of them used providing information, controling respiration and supportive touch. 2. The effective nursing intervention used in their field are speaking in soft quiet tones, pacifiers and nesting with blanket in the group of younger than one year old. Un the group of 1-6 years old, speaking in soft quiet tones, toys, and supportive touch were effective method in the control of nonp-harmacologic pain management. In the group of 6-12 years old, story talking, supportive touch, and speaking in soft quiet tones were effective method and in the group of 12-18 years old, providing information, cold therapy and supportive touch were effectively used to control nonpharmacologic pain management. 3. To compare the general characteristics and non-pharmacologic pain nursing intervention, in the group of younger than one year, touching stimuli is widely used. In the groups of 1-6, and 6-12 years old, visual and audio method were widely used. In the group of 12-18 years old, sensitive intervention were used as well as education, information and guided imagery. In conclusion, there was no significant difference in nurses' demographic characteristics, child's age and nonpharmacologic pain management. There was significant difference only in the nurses working area, that is nurses working in the surgical department used more audio-visual-tactile pain management methods than medical department.

• Therapeutic Science for Rehabilitation
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• v.8 no.1
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• pp.63-71
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• 2019

Objective: This study aimed to examine the factors affecting bodily pain in women in their middle to older age who are caregivers of stroke patients. Methods: This study selected 75 stroke inpatients and 75 female caregivers in their middle to older age from five hospitals in Daegu and Gyeongbuk. Measurements included general characteristics, cognitive function, upper limb function, and activities of daily living of stroke patients, and general characteristics and bodily pain for the caregivers. We used stepwise multiple regression analysis to determine the factors affecting the bodily pain and Pearson's correlation analysis to confirm the correlation. Results: The factors affecting bodily pain in women in their middle to older age caregivers were the activities of daily living(${\beta}=-.489$, p<.001) and caregiver duration(${\beta}=-.309$, p=.003)($R^2=.276$). Correlation analysis showed that the activities of daily living, upper limb function, and cognitive function of stroke patients were significantly correlated (r=.434~.751, p<.001). Conclusions: The results of this study demonstrate that the functional level of stroke patients being cared for and the caregiver duration are important variables for reducing bodily pain in women in their middle to older age.

• Journal of the Korea Convergence Society
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• v.12 no.8
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• pp.1-11
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• 2021

The purpose of this study was to analyze the trends and characteristics of 'stress urinary incontinence' research through word frequency analysis, and their relationships were modeled using word embedding. Abstract data of 9,868 papers containing abstracts in PubMed's MEDLINE were extracted using a Python program. Then, through frequency analysis, 10 keywords were selected according to the high frequency. The similarity of words related to keywords was analyzed by Word2Vec machine learning algorithm. The locations and distances of words were visualized using the t-SNE technique, and the groups were classified and analyzed. The number of studies related to stress urinary incontinence has increased rapidly since the 1980s. The keywords used most frequently in the abstract of the paper were 'woman', 'urethra', and 'surgery'. Through Word2Vec modeling, words such as 'female', 'urge', and 'symptom' were among the words that showed the highest relevance to the keywords in the study on stress urinary incontinence. In addition, through the t-SNE technique, keywords and related words could be classified into three groups focusing on symptoms, anatomical characteristics, and surgical interventions of stress urinary incontinence. This study is the first to examine trends in stress urinary incontinence-related studies using the keyword frequency analysis and word embedding of the abstract. The results of this study can be used as a basis for future researchers to select the subject and direction of the research field related to stress urinary incontinence.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.101-101
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• 2003

Main strategy for a treatment of Parkinson's disease (PD), due to a progressive degeneration of dopaminergic neurons, is a pharmaceutical supplement of dopamine derivatives or ceil replacement therapy. Both of these protocols have pros and cons; former exhibiting a dramatic relief but causing a severe side effects on long-term prescription and latter also having a proven effectiveness but having availability and ethical problems Embryonic stem (ES) cells have several characteristics suitable for this purpose. To investigate a possibility of using ES cells as a carrier of therapeutic gene(s), human ES (hES, MB03) cells were transfected with cDNAs coding for tyrosine hydroxylase (TH) in pcDNA3.1 (+) and the transfectants were selected using neomycin (250 $\mu /ml$). Expression of TH being confirmed, two of the positive clone (MBTH2 & 8) were second transfected with GTP cyclohydrolase 1 (GTPCH 1) in pcDNA3.1 (+)-hyg followed by selection with hygromycin-B (150 $\mu /ml$) and RT-PCR confirmation. By immune-cytochemistry, these genetically modified but undifferentiated dual drug-resistant cells were found to express few of the neuronal markers, such as NF200, $\beta$-tubulin, and MAP2 as well as astroglial marker GFAP. This results suggest that over-production of BH4 by ectopically expressed GTPCH I may be involved in the induction of those markers. Transplantation of the cells into striatum of 6-OHDA- denervated PD animal model relieved symptomatic rotational behaviors of the animals. Immunohistochemical analyses showed the presence of human cells within the striatum of the recipients. These results suggest a possibility of using hES cells as a carrier of therapeutic gene(s).

• Korean Journal of Family Social Work
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• no.23
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• pp.263-302
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• 2008

The study had four purposes as follows; First, the researcher tried to find the characteristics of conflict. Second, the researcher attempted to find the factors influencing the conflict between mother and the eldest son, Third, the study attempted to find the therapist's intervention techniques, which were caused the change of the conflict between mother and the eldest son. Lastly, the study was to find the changed contents of family relationships. The total family counseling was 13 sessions, which included individual and family counseling. The study used constant comparative analysis by using open coding method to find the factors. Also, the researcher used Miles and Huberman's matrix and graphical network display to show the relationships among factors influencing the conflicts between mother and the eldest son. The study found the boundary problem of family, family's undifferentiated ego, dysfunctional communication patterns, and mother's experience from family of origin as factors influencing the conflict between mother and the eldest son. On the other hand, the study found that the therapist used the formation of therapeutic alliance relationship, guide of communication skills, the reflection of repeated pattern, reframing and a metaphor as intervention methods. The result of study revealed that changed level of family relationship, activating functional communication, changed boundary among family members, differentiated ego of family members, and changed cognition as the changed contents of family relationship.

• Tuberculosis and Respiratory Diseases
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• v.73 no.6
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• pp.303-311
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• 2012

Background: This study was designed to analyze the efficacy of gefitinib as a second-line therapy, according to the clinical characteristics in Korean patients with non-small-cell lung cancer (NSCLC). Methods: In this Phase IV observational study, we recruited patients, previously failed first-line chemotherapy, who had locally advanced or metastatic NSCLC, and who were found to be either epidermal growth factor receptor (EGFR) mutation-positive or satisfied 2 or more of the 3 characteristics: adenocarcinoma, female, and non-smoker. These patients were administered with gefitinib 250 mg/day, orally. The primary endpoints were to evaluate the objective response rate (ORR) and to determine the relationship of ORRs, depending on each patient's characteristics of modified intent-to-treat population. Results: A total of 138 patients participated in this study. One subject achieved complete response, and 42 subjects achieved partial response (ORR, 31.2%). The subgroup analysis demonstrated that the ORR was significantly higher in patients with EGFR mutation-positive, compared to that of EGFR mutation-negative (45.8% vs. 14.0%, p=0.0004). In a secondary efficacy variable, the median progression-free survival (PFS) was 5.7 months (95% confidence interval, 3.9~8.4 months) and the 6-month PFS and overall survival were 49.6% and 87.9%, respectively. The most common reported adverse events were rash (34.4%), diarrhea (26.6%), pruritus (17.5%), and cough (15.6%). Conclusion: Gefitinib was observed in anti-tumor activity with favorable tolerability profile as a second-line therapy in these selected patients. When looking at EGFR mutation status, EGFR mutation-positive showed strong association with gefitinib by greater response and prolonged PFS, compared with that of EGFR mutation-negative.