• 생약학회지
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• 제29권2호
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• pp.136-141
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• 1998

To analyze scientifically the polyprescription principle of Daekumeumja(對金飮子), which has been used for alcoholic damage, and Pyungwesan(平胃散), which has been used for indigestion, the transforming rate of hesperidin of these polyprescriptions to hesperetin was investigated. The transforming rate of the former was higher 3 times than that of the latter. The transforming rate of hesperidin of Aurantii nobilis Pericarpium was inhibited by Magnoliae Cortex, but was activated by Glycyrrhizae Radix. The activity of trypsin was inhibited by Glycyrrhizae Radix and Daekumeuja. However, Aurantii nobilis Pericarpium, Atractylodis Rhizoma, Magnoliae Cortex and Pyungwesan did not inhibited it. When human intestinal microflora were cultured with the media containing Daekumeumja, Pyungwesan and herbal medicines consisting of them, Aurantii nobilis Pericarpium and Magnoliae Cortex inhibited the activity of ${\beta}$-glucosidase. These results suggests that the therapeutic effect of Daekumeumja may be better than that of Pyungwesan on alcoholic damage and the therapeutic effect of Pyungwesan may be better than that of Daekumeumja on indigestion, although these prescriptions are consisted of the same herbal medicines.

• 트랜스-
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• 제16권
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• pp.1-31
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• 2024

디지털 매체의 발전은 청소년들이 마약류 구매 환경에 쉽게 접근하며 소셜 네트워크 서비스(SNS)와 메신저 서비스를 통한 마약류 구매 및 복용 사례가 증가하고 있다. 이러한 환경에 노출된 청소년들은 마약류 중독으로 인한 신경학적 및 정신 건강 문제를 겪을 위험이 있으며, 이는 범죄에 노출될 위험을 증가시킨다. 따라서, 국가 차원에서의 관리와 지원이 절실히 필요하다. 마약에 노출된 청소년들을 위한 지속 가능한 치료 방안을 모색하는 것은 중대한 과제로 부상하고 있다. 재발 위험이 높은 마약류 중독 치료를 위해선 비용이 효율적이며 사용자 친화적인 치료 프로그램이 필요하다. 본 연구는 디지털 플랫폼을 활용하여 청소년들이 자발적으로 참여할 수 있는 치료 환경을 조성하고, 예술을 활용한 치료적 콘텐츠 개발을 목표로 하는 문헌 연구를 수행한다. 청소년 약물 중독에 대한 사회적 인식과 치료 현황을 검토하고, 마약류 중독이 청소년의 뇌 활동 및 인지 기능 저하에 미치는 영향을 분석하여, 중독된 뇌 기능의 재활을 촉진할 수 있는 디지털 치료제 개발에 관한 방안을 선행 연구 사례 분석을 통해 모색한다. 또한, 디지털 치료적 접근법과 예술치료의 통합이 치료 과정에 어떠한 이점을 제공할 수 있는지를 탐구하며, 연극 치료, 음악 치료, 미술치료 등 다양한 치료 프로그램이 청소년에게 미치는 치료적 효과의 증대 가능성을 제안한다. 예술 치료 요법의 적용은 도구의 확장, 표현의 다양화, 데이터의 확보, 동기 부여 측면에서 긍정적 효과를 기대할 수 있다. 이러한 접근을 통해 청소년 마약류 중독 치료의 효과성이 증대될 것으로 예상된다. 종합적으로 볼 때, 본 연구는 청소년 마약류 중독에 대한 사회적 상황을 고려한 경제적이며 지속 가능한 치료 방안을 제공하는 디지털 치료제 및 관련 애플리케이션 개발을 위한 기초 연구를 수행하고자 한다.

• The Korean Journal of Pain
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• 제19권2호
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• pp.181-186
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• 2006

Background: Although cervical epidural block can be a useful therapeutic treatment for head, neck and upper extremities pain, there is no consensus regarding the volume of injection required for pain management. Herein, the spreading in the vertebral segments after a cervical epidural injection of either a 5 or 10 ml volume was studied. Methods: A total of 78 patients, suffering from head, neck and upper extremity pain, were selected. Cervical epidural blocks were performed consecutively with 5 ml (n = 42) and 10 ml (n = 36) of 0.4% mepivacaine and 222 mg I/ml iopamidol at the C7⁣-T1 levels. Both anteroposterior (AP) and lateral radiographs were obtained under fluoroscopy, and the upper and lower epidural spreading of the contrast media in relation to the vertebral level was evaluated. Results: The cervical epidural blocks were performed without complications. The rostral spreading of the contrast media in the vertebral segments in groups 1 and 2 were $5.6{\pm}1.1$ and $6.1{\pm}1.1$, respectively. The caudal spreading of the contrast media in the vertebral segments in groups 1 and 2 were $5.4{\pm}3.4$ and $7.2{\pm}3.9$, respectively. The total numbers of segments with vertebral spreading of the contrast media in both directions showed significant differences between the two groups. The numbers of patients who showed spreading of the contrast media up to C2 vertebral segment showed no significant differences between the two groups. Conclusions: 5 and 10 ml epidural injection volumes may be adequate for the spread of contrast media to the entire cervical spine. A 5 ml epidural injection volume, compared to a 10 ml volume, may be ample when considering the possibility of unnecessary caudal spreading of drugs and volume related complications in the management of head, neck and upper extremity pain.

• 대한임상검사과학회지
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• 제39권1호
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• pp.19-24
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• 2007

The aim of this study was to evaluate the bacterial effects of Moraxella catarrhalis in otitis media with effusion (OME) by photodynamic therapy (PDT). Bacterial suspensions (10000 CFU/mL) were prepared. The colony forming units (CFU) of Moraxella catarrhalis have been measured after an application of photogem plus 632 nm diode laser irradiation. One ml of the bacterial suspensions have been incubated in the dark for 3h with various concentrations of photogem ($0.625{\sim}5.0_{\mu}g/mL$) and then irradiated with 632 nm diode laser ($15J/cm^2$). After, the PDT Moraxella catarrhalis suspensions ($50{\mu}L$) were inoculated on chocolate agar plate and cultured in the dark at $37^{\circ}C$, 5% $CO_2$ condition for 18h. The colony forming units off the bacteria were measured. Also transmission electron microscopy (TEM) was employed to evaluate the effect of otitis media pathogens by PDT. The nucleus of Moraxella catarrhalis was stained using green fluorescent nucleic acid dye thiazole orange and the fluorescence intensity of the nucleus was measured by flow cytometry. The PDT was effective in killing Moraxella catarrhalis at the photogem dose of $5.0_{\mu}g/mL$, respectively, As assessed by flow cytometry analysis the fluorescence intensity of the nucleus got lower after PDT. TEM result appeared to able to cause damage to the bacterial membranes. On the basis of these findings, bacterial photodynamic therapy with photogem can be considered to be a promising new therapeutic approach for OME.

• 문화기술의 융합
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• 제8권6호
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• pp.67-77
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• 2022

본 연구에서는 디지털가면을 화상상담에 활용한 가면화상상담을 진행한 상담자들의 경험을 탐색하는데 그 목적이 있다. 이를 위하여 총 10사례에 대한 가면화상상담을 경험한 4명의 상담사와의 초점집단면접 자료가 주제분석방법을 활용하여 분석되었다. 상담사들은 가면화상상담에 대한 우려가 있었으나 점차 적응해나갔으며 편안함과 재미를 느꼈다. 그러나 화상상담에서보다 더 큰 피로와 부담감을 느껴 가면화상상담을 위한 추가적인 교육훈련이 필요하다고 보았다. 또한 내담자들의 신속한 자기개방과 상담과정을 관찰하면서 디지털가면이 상담매체로 활용될 가능성을 긍정적으로 전망하였다. 본 연구의 결과에 기초하여 우리는 온라인 상담매체 활용을 위한 상담자 지원이 필요함을 제언하였다.

• 한국동물생명공학회지
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• 제38권3호
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• pp.109-120
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• 2023

Background: Pluripotent stem cells (PSCs) including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) offer the immense therapeutic potential in stem cell-based therapy of degenerative disorders. However, clinical trials of human ESCs cause heavy ethical concerns. With the derivation of iPSCs established by reprogramming from adult somatic cells through the transgenic expression of transcription factors, this problems would be able to overcome. In the present study, we tried to differentiate porcine iPSCs (piPSCs) into endothelial cells (ECs) for stem cell-based therapy of vascular diseases. Methods: piPSCs (OSKMNL) were induced to differentiation into ECs in four differentiation media (APEL-2, APEL-2 + 50 ng/mL of VEGF, EBM-2, EBM-2 + 50 ng/mL of VEGF) on cultured plates coated with matrigel^® (1:40 dilution with DMEM/F-12 medium) for 8 days. Differentiation efficiency of these cells were exanimated using qRT-PCR, Immunocytochemistry, Western blotting and FACS. Results: As results, expressions of pluripotency-associated markers (OCT-3/4, SOX2 and NANOG) were higher observed in all porcine differentiated cells derived from piPSCs (OSKMNL) cultured in four differentiation media than piPSCs as the control, whereas endothelial-associated marker (CD-31) in the differentiated cells was not expressed. Conclusions: It can be seen that piPSCs (OSKMNL) were not suitable to differentiate into ECs in the four differentiation media unlike porcine epiblast stem cells (pEpiSCs). Therefore, it would be required to establish a suitable PSCs for differentiating into ECs for the treatment of cardiovascular diseases.

• KSBB Journal
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• 제21권4호
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• pp.306-311
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• 2006

세포의 보존은 세포의 배양에 있어서 필수 불가결한 요건으로서 세포주의 다양한 특성에 따라 적합한 방법이 확립되어야 한다. 본 연구에서는 산업용 세포주인 CHO 세포의 단기간 저온보존 기술의 확립을 목표로 진행되었으며 다양한 조건을 통해 가장 안정적인 저온보존 방법을 수립하였다. 저온보존 방법에 있어서 가장 중요한 요인은 온도로서 $4^{\circ}C$ 저온보존이 세포 보존에 필수적인 조건으로 나타났으며 $20^{\circ}C$ 실온보존에서는 세포의 급격한 사멸이 관찰되었다. 보존형태는 용기를 눕힌 상태로 서서히 회전시켜 현탁 보존하는 방법이 용기를 세우거나 눕혀 보관하는 방법에 비해 높은 생존율을 나타내었다. 또한 저온보존 시 새로운 배지로 교환한 후 보존하는 방법이 배양에 사용된 배지를 그대로 사용한 보존 방법보다 세포의 성장 회복율에서 우수한 것으로 나타났다. 하지만 $4^{\circ}C$에서 rolling을 통한 현탁 보존을 할 경우에는 배지의 교환 없이도 안정적으로 세포보존이 가능한 것으로 나타났다. 저온보존에 가장 적합한 세포의 농도는 실험결과 $1.0{\times}10^6{\sim}5.0{\times}10^6cells/m{\ell}$ 범위로 나타났으며 혐기적인 상태로 보존하는 것이 공기가 존재하는 보존방법 보다 비교적 우수한 보존 결과를 나타내었다. 이상의 결과를 바탕으로 무혈청 배지의 저온보존액으로서의 안정성과 첨가물에 의한 보존효율의 향상을 평가하였다. 실험결과 저온보존 후 10일간은 높은 세포 생존율과 함께 정상적인 세포 성장 회복을 보이는 것으로 나타났으며 ${\alpha}$-tocopherol과 retinoic acid를 첨가한 저온보존액의 경우에는 더욱 우수한 세포 생존율을 보임을 확인하였다. 마지막으로 이렇게 확립된 방법을 이용하여 1 L 용량의 저온보존 실험을 수행한 결과, 앞선 실험에서와 유사한 경향의 세포 보존 능력을 확인할 수 있었다. 이러한 결과를 종합해 볼 때 산업용 세포주로 널리 사용되는 CHO 세포의 저온보존은 본 연구에서 확립된 방법을 통해 단기간 동안 안정적으로 수행될 수 있을 것으로 사료되며 대용량 저온보존의 적용 가능성도 확인하였다. 대용량 배양에서의 단기간 보존기술에 대한 연구가 앞으로 더 많이 수행된다면 실제 배양 공정에서도 저온보존 기술의 적용이 가능할 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권11호
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• pp.1644-1651
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• 2014

Transgenic animals have become important tools for the production of therapeutic proteins in the domestic animal. Production efficiencies of transgenic animals by conventional methods as microinjection and retrovirus vector methods are low, and the foreign gene expression levels are also low because of their random integration in the host genome. In this study, we investigated the homologous recombination on the porcine ${\beta}$-casein gene locus using a knock-in vector for the ${\beta}$-casein gene locus. We developed the knock-in vector on the porcine ${\beta}$-casein gene locus and isolated knock-in fibroblast for nuclear transfer. The knock-in vector consisted of the neomycin resistance gene (neo) as a positive selectable marker gene, diphtheria toxin-A gene as negative selection marker, and 5' arm and 3' arm from the porcine ${\beta}$-casein gene. The secretion of enhanced green fluorescent protein (EGFP) was more easily detected in the cell culture media than it was by western blot analysis of cell extract of the HC11 mouse mammary epithelial cells transfected with EGFP knock-in vector. These results indicated that a knock-in system using ${\beta}$-casein gene induced high expression of transgene by the gene regulatory sequence of endogenous ${\beta}$-casein gene. These fibroblasts may be used to produce transgenic pigs for the production of therapeutic proteins via the mammary glands.

• Pediatric Infection and Vaccine
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• 제16권1호
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• pp.6-12
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• 2009

There are relatively few novel antimicrobial agents despite the dramatic increase in antimicrobial resistance and multiple drug resistance of clinical isolates worldwide. Vancomycin is still the most widely used antibiotic for treating resistant Gram-positive coccal infections in children, especially for methicillin-resistant Staphylococcus aureus. For children with Gram-positive coccal infections where vancomycin is not effective or older therapeutic agents cannot be tolerated, linezolid, quinupristin-dalfopristin or daptomycin may be useful in the appropriate clinical setting. For Gram-negative bacterial infections, new carbapenems await clinical application. Tebipenem pivoxil is a novel oral carbapenem undergoing clinical trials for acute otitis media in pediatric patients. Antiviral drug development is now progressing at the pace of antibiotic development 30 years ago. Newer antiviral agents used for the treatment of herpes viruses and hepatitis C virus infections in children are included in this review.

• Journal of Microbiology and Biotechnology
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• 제23권9호
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• pp.1327-1338
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• 2013

The humanized anti-hepatocyte growth factor (HGF) monoclonal antibody (mAb) YYB-101 is a promising therapeutic candidate for treating various cancers. In this study, we developed a bioprocess for large-scale production of YYB-101 and evaluated its therapeutic potential for tumor treatment using a xenograft mouse model. By screening diverse chemically defined basal media formulations and by assessing the effects of various feed supplements and feeding schedules on cell growth and antibody production, we established an optimal medium and feeding method to produce 757 mg/l of YYB-101 in flask cultures, representing a 7.5-fold increase in titer compared with that obtained under non-optimized conditions. The optimal dissolved oxygen concentration for antibody production was 70% $pO_2$. A pH shift from 7.2 to 7.0, rather than controlled pH of either 7.0 or 7.2, resulted in productivity improvement in 5 L and 200 L bioreactors, yielding 737 and 830 mg/ml of YYB-101, respectively. The YYB-101 mAb highly purified by affinity chromatography using a Protein A column and two-step ion exchange chromatography effectively neutralized HGF in a cell-based assay and showed potent tumor suppression activity in a mouse xenograft model established with human glioblastoma cells.