• Journal of Forest and Environmental Science
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• v.36 no.4
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• pp.290-297
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• 2020

This study analyzed and compared the damage rate, natural parasitic rate, and the morphological characteristics of Thecodiplosis japonensis larvae, which inhabit forest areas as control areas to large urban areas in 2018 and 2019. This research was conducted to provide basic data for the management of Thecodiplosis japonensis, which harm pine needles, and the results were as follows. First, the gall formation rate of Thecodiplosis japonensis collected from urban areas was upper-crown 35.59% and 34.25%, mid-crown 25.57% and 27.95%, and lower-crown 25.34% and 26.61%; the gall formation rate of Thecodiplosis japonensis was in the order of upper-crown>mid-crown>lower-crown in 2018 and 2019, respectively. In the control areas, the gall formation rates of Thecodiplosis japonensis in mountain villages in 2018 and 2019 were upper-crown 17.72% and 21.78%, mid-crown 13.85% and 16.97%, and lower-crown 15.12% and 15.79%; thus, in the order of upper-crown>lower-crown>mid-crown. The number of larvae in the galls of needles damaged by Thecodiplosis japonensis was as follows: the average number of larvae in the pine trees of urban areas was 9 and 8 in the upper-crown, 7 and 8 in the mid-crown, and 6 and 7 in the lower-crown respectively. This shows that the number of larvae was fewer in the lower-crown than the upper-crown, and that the number of larvae was higher in 2018 than in 2019. For natural parasitic rate of Thecodiplosis japonensis, the gall formation rate and natural parasitic rate of Thecodiplosis japonensis were surveyed; the natural parasitic rate was 12.5% and 11.8% in urban areas while the rate was 21.7% and 20.9% in mountain villages in respectively in 2018 and 2019.

• Korean journal of applied entomology
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• v.34 no.4
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• pp.368-372
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• 1995

To develop a microbial pesticide for the control of pine gall midges. Thecodiplosis japonensis, entomopathogenc fungi were isolated from 233 soil samples in the damaged region of Thecodiplosis japonenesis, and identified with Beauveria spp. 29 strains and Paecilomyces spp. 2 strains. The morphology of entomopathogenic fungi was observed by scanning electron miroscope. In addition, the toxicity of entomopathogenic fungi was observed by scanning electron microscope. In addition, the toxicity of entomopathogenic fungi isolated from soil samples was determined by bioassay against Thecodiplosis japonensis larvae. The result showed that toxicity of relatively pathogenic strains, Beauveria spp. SFB-168-2 was 82.9%, suggesting that Beauveria spp. SFB-168-2 is effective entomopathogenic fungi for the control of pine gall midges.

• Korean journal of applied entomology
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• v.27 no.3
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• pp.144-148
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• 1988

Using scanning electron microscope, we studied the adult genital segments of pine needle gall midge, Thecodiplosis Japonensis, Uchida et Inouye Male: There were two pairs of hypoproct a pair of claspettes and aedeagus, Hypoproct were surrounded with many setae and sensilla. Clasperres which was projected below hypoproct was composed of two aegments and had many sensilla Aedeagus was long and flattened and possesed some sensilla at the terminal. Female :Ovipositor was segmented into three parts. Each segment had a different externla sturcture; particularly, the 2nd one was covered with reversed boat-like attachments and did not show any sensillum.

• Microbiology and Biotechnology Letters
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• v.27 no.5
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• pp.415-418
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• 1999

Thecodiplosis japonensis is an important insect pest in pine trees and hard to be controlled by chemical pesticides due to its characteristic life cycle. Experiments were conducted to determine the pathogenicity and growth temperature of several entomopathogenic fungi as potential microbial insectcides for T. japonensis. Beauveria bassiana SFB-SC and Paecilomyces fumosoroseus SFP-198 were highly pathogenic against T. japonensis. B. bassiana SFB-SC had also pathogenicity against a beneficial insect Bombyx mori. Growth rate and pathogenicity of P. funmosoroseus SFP-198 under 15$^{\circ}C$ was higher than those of B. bassians SFB-SC. These results indicated that P. fumosoroseus SFP-198 is a potential microbial control candidate for control of T. japonensis.

• Korean journal of applied entomology
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• v.29 no.1
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• pp.25-30
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• 1990

Physiological and morphological differences in larvae and female adults of Thecodiplosis Japonensis from Haenam, Chullanamdo, and Chunsung, Kangweondo, were studied by means of electrophoretic technique and scanning electron microscope (SEM). On zymograms of whole body, third instar larvae of T. japonensis showed geographic differences in the band patterns of esterase and MDH iszymes, but patterns and the staining density of general proteins were similar in tow populations. In female adults, the populations revealed geographic differences in general proteins and esterase isozymens. In external ultrastructures, especially in genital segments, each population had distinctive structures in the 2nd segment of ovipositor.

• Journal of the Korean Professional Engineers Association
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• v.10 no.4
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• pp.34-39
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• 1977

• Korean journal of applied entomology
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• v.4
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• pp.55-58
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• 1965

This is a report that we investigated the distribution of Pine Gall-midge (Thecodiplosis japonensis UCHIDA et INOUYE) along the main roads in the vicinity of Seoul. 1. The Pine Gall-midge around the districts of Seoul spread to East-Northern direction, Kwang-Ju, Yang-Su, Su-Pa, Bong-il-Chun and Ko-Chon etc. 2. The factors responsible to the dispersal of Pine Gall-midge seemed to be with the direction of the wind during the period of adult emergence. 3. The parasitic rate of Isostasius seoulis on the Pine Gall-midge was very low except $13.6\%$ at Su-Pa district.

• Journal of Forest and Environmental Science
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• v.16 no.1
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• pp.34-41
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• 2000

This study investigated tree vigor of damaged forest by Thecodiplosis japonensis Uchida et Inouye. In damaged forest by pine gall midge, the investigation result of damaged rate section (severe, moderate, light) indicated that severe stand showed higher value of electric resistance ($k{\Omega}$) than that of light stand, the investigation result of tree density section(crowed, middle, little) indicated that crowed stand showed higher value of electric resistance than that of little stand (the value of electric resistance at which low reading show high vigor tree and high reading low vigor tree). The crown width and DBH (diameter of breast height) of external factors showed lower value of electric resistance than that of small size. The results of comparative between Crown area and value of electric resistance in each stand were $13.4m^2$ in severe stand, $10.9m^2$ in moderate stand and $7.9m^2$ in light stand. Therefore, the tree which have big crown showed low value of electric resistance (below $15k{\Omega}$)that has high possible of survial.

• Korean journal of applied entomology
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• v.15 no.4 s.29
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• pp.169-178
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• 1976

The concentration of amino acids, total nitrogen, trehalose, lipids and the activities of respiratory, acid$\cdot$alkaline phosphatase, glutamic oxalozcetic transaminase and glutamic pyruvic transaminase during larval stage in Pine leaf gall midge, Thecodiplosis janensis Uchi. et Inouye were measured using Paper chromatographic method, micro-Kjeldahl method, Thin layer chromatographic method, Warburg's manometric method, Bessey-Lowry method and Reitman-Frankel method, respectively. Healthy specimens )yore chosen as samples of each larval stages; alrva in gall and larva in soil. Amino acids present in the alcoholic extracts were alanine, glutamic acid, glycine, histidine, methionine, proline, threonine, tryptophan and valine. The total nitrogen concentration reached to 31.348mg/g during the larva in gall and the larval stage in soil of the value was decreased to 29.027mg/g. The hemolymph sugar, trehalose value for larva in soil was about two times of the value for larva in gall. Total lipid, phospholipid,monoacylglycerol, triacylglycerol, sterol, free fatty acid and ester cholesterol were identified at larval stages in gall and soil. Triacylglycerol concentration reached high level in contrast with other lipid contents during larvae in gall and larva in soil. Free fatty acid, sterol except decreased lipids during larval stage in soil. Endogenous respiration, succinate of respiratory activities decreased at larval stage in soil compare with larva in gall. The activities of acid phosphatase decreased larval stage in soil but the activities of alkaline phosphatase increased remarkably. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase reached high level of the larva in gall.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.1
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• pp.77-80
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• 2004

For the development of the alternative control system against the major forest pests, Beauveria spp. F-101, isolated from a dead larva of Thecodiplosis japonensis, was selected because this isolate showed high pathogenicities against T. japonensis and Acantholyda parki. Beauveria spp. F-101 had irregular clustered conidio-phores and conidia borne on a distinctive apical zigzag extension, and it showed typical characteristic of the genus, Beauveria in morphology. For molecular based-identification, the ribosomal ITS region of Beauveria spp. F-101 was amplified with ITS1 and ITS4 primers, and cloned into pGEM- T Easy vector. The amplified PCR product was 569 bp in size and completely sequenced. The similarities of the cloned ITS sequence were 99 ％ and 97％ to those of B. bassiana and B. brongniartii, respectively. In comparison to other species among the genus Beauveria, the ITS region of Beauveria spp. F-101 showed a similarity of 95％ to B. amorpha, 95％ to B. tenella, 89％ to B. vermiconia and 69％ to B. alba, respectively. In addition, in comparison to different genus, it had 95％ similarities to Cordyceps militaris and 91％ to Paecilomyces tenuipes. Accordingly, the current result suggests that Beauveria spp. F-101 was a variant of B. bassiana and it seems to be a new isolate considering sequence variation in ITS region.