• 한국미생물·생명공학회지
• /
• 제34권4호
• /
• pp.289-298
• /
• 2006

3',5'-cyclic adenosine monophosphate (cAMP) is an important molecule, which mediates diverse cellular processes. For example, it is involved in regulation of sugar uptake/catabolism, DNA replication, cell division, and motility in various acterial species. In addition, cAMP is one of the critical regulators for syntheses of virulence factors in many pathogenic bacteria. It is believed that cAMP acts as a signal for environmental changes as well as a regulatory factor for gene expressions. Therefore, intracellular concentration of cAMP is finely modulated by according to its rates of synthesis (by adenylate cyclase), excretion, and degradation (by cAMP phosphodiesterase). In the present review, we discuss the bacterial physiological characteristics governed by CAMP and the molecular mechanisms for gene regulation by cAMP. Furthermore, the effect of cAMP on phosphotransferase system is addressed.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제15권3호
• /
• pp.814-836
• /
• 2021

Wildlife ecologists and biologists recapture deer to collect tracking data from deer collars or wait for a drop-off of a deer collar construction that is automatically detached and disconnected. The research teams need to manage a base camp with medical trailers, helicopters, and airplanes to capture deer or wait for several months until the deer collar drops off of the deer's neck. We propose an intelligent robust base-station research with a low-cost and time saving method to obtain recording sensor data from their collars to a listener node, and readings are obtained without opening the weatherproof deer collar. We successfully designed the and implemented a robust base station system for automatically collecting data of the collars and listener motes in harsh wilderness environments. Intelligent solutions were also analyzed for improved data collections and pattern predictions with drone-based detection and tracking algorithms.

• 한국비블리아학회지
• /
• 제21권2호
• /
• pp.39-56
• /
• 2010

이 연구는 공공도서관 청소년 프로그램의 현황을 조사하여 문제점을 분석하고 개선 과제를 탐색하였다. 1개 특별시와 6개 광역시의 공공도서관 중 2009년 8월 31부터 9월 4일까지 청소년 프로그램이 실시된 86개관을 대상으로 조사하였다. 이 연구에서는 청소년프로그램을 참여형, 체험형, 강좌형, 콘테스트형, 기관협력형 등 5가지 유형으로 구분하여 각 유형별 특성과 문제점을 분석하였다. 그리고 공공도서관의 청소년 프로그램의 활성화를 저해하는 제반 환경적 문제를 점검하고 발전 방안을 제안하였다.

• 대한기계학회논문집A
• /
• 제25권10호
• /
• pp.1575-1582
• /
• 2001

Injection molding is widely used in producing various plastic parts due to its high productivity, and the demand for injection molded products with high precision is increasing. To achieve successful product quality and precision, the design of gating and runner system in injection mold is very important because it influences the melt flow into the cavity. Some deflects, such as weld lines and overpacking, can be effectively controlled with proper selection of gate locations. In the present study, the design of gate locations in injection molding of a dashboard fur automobiles was carried out with CAMP mold, a PC-based simulation system for injection molding. A dummy runner system was developed to simulate a runner system in order to increase the efficiency of the analysis procedure. The numbers and locations of gates were iteratively determined in the present investigation. In this procedure, an acceptable design was obtained in terms of reducing the maximum pressure and clamping force.

• 생명과학회지
• /
• 제17권10호
• /
• pp.1361-1367
• /
• 2007

Nocardiopis sp. 유래의 protein kinase 저해제인 KT5720, KT5926을 사용하여 Vesicular Stomatitis Virus (VSV) 증식에 미치는 세포변성의 억제를 지표로서 연구하였다. 그 결과 CAMP의존 kinase를 저해하는 KT5720, mysion light chain kinase (MLCK)를 저해하는 KT5926이 VSV에 의한 세포변성을 억제시켰다. Protein kinase inhibitor KT5720은 VSV의 증식에 영향을 미치지는 않지만, 세포변성과정은 억제하는 것으로 나타났다. Virus RNA 및 단백질 합성에서 PKA KT5720이 영향을 미치지 않는 것은 virus 증식에 영향이 없는 것으로 나타난다. Virus 증식과정에서 성질이 다른 protein kinase가 관여하는데, 이것은 negative strand virus에 대한 항virus 제 개발에 중요한 역할을 한다고 생각한다.

• 한국지역사회생활과학회지
• /
• 제15권3호
• /
• pp.29-43
• /
• 2004

The purpose of this study was to calculate the economic effect of Women-Farmer's Center. Since 2001, The Ministry of Agriculture and Forestry has run Women-Farmer's Centers in which women-farmers can receive the care for their pre-schooling children, after-school learning service, and city-farm exchange, education, and counseling. In other words, Women Farmer's Center provides not only improvement of ease and quality of life of women-farmer's, but also spreading economic effect to community and country. By calculation based on business plan of 14 centers that run centers, total economic income effects are 2,784 million won, which consist of 1,265 million won for counseling, 146 million won for the care of infants and children, 139 million won after-school learning, 1,020 million won for education, and 214 million won for city-farm exchange program. The Women-Farmer's Center should be managed reasonably with government support so that Women-Farmer's Center will become as a base camp for young women farmers to participate in agriculture and rural community and increase its economic effect for the nation in the future.

• 한국초등과학교육학회지:초등과학교육
• /
• 제31권2호
• /
• pp.177-187
• /
• 2012

The purpose of this study was to acquire a better of how creativity center is perceived among students, teachers and parents by administering a questionnaire. A total of 320 people participated in this survey. We developed the questionnaire which as categorized by four specific areas: 1) people's overall perceptions of creativity education; 2) the way of using the creativity center 3) programing of creativity center 4) space composition of the creativity center. The summary of survey results included; firstly, people had paid attention to creativity education and creativity education; secondly, they want to make use of creativity center with informal learning and extra-curriculum; thirdly, they prefer to participate in many experience activities such as camp or experiments; lastly, they more need creativity experience program and utilization of DIY education. In conclusion, students, teachers and parents recognized creativity center is needed. Therefore, more intensive research and follow-up study on building creativity center should be highly considered.

• Preventive Nutrition and Food Science
• /
• 제3권2호
• /
• pp.157-162
• /
• 1998

As a part of investigation for listeriosis, we attmepted iolationof Listeria spp. from commerical frozen and refrigerated foods at the supermarket level. Seven strains of Listeria spp. were isolate from 6 samples (7.15) among 84 samples of frozen foods, and ten strains of Listeria spp. were also isolated from 8 samples (7.6%)_ among 105 samples of refrigerated foods at the supermarkets in Pusan area. From a total of 189 commerical foods, the number of isolated Listeria spp. and ratio were 6 strains(3.25) of L. grayi, one strain (0.5%) of L. welshimeri, 6 strains (3.2%) of L. innocua and 4 strains (2.15) of L monocytogenes. Listeria spp. isolates except L. monocytogenes did not show $\beta$-hemolysis on blood agar and positive reaction in CAMP test with Staphyloccccus aureus. In the antibiotic susceptibility,most isolates of Listeria spp. were susceptible to 12 antibiotics such as ampicillin , cephalotin, penicillin, amikacin, gentamicin, erythromycin, kanamycin, vancomycin, tobramycin, carbenicillin , tetracycline and trimethoprim /sulfamethoxiazole. Four strains of L. monocytogenes were susceptible to ntibiotics used in this study except nitrofurantio. The serotype of 3 strains and one strain of L. monocytogenes were classified into Listeria O serotype 1 and 4, respectively.

• 생명과학회지
• /
• 제16권7호
• /
• pp.1225-1228
• /
• 2006

cAMP와 복합체를 형성한 cAMP 수용성 단백질은 DNA와 결합하여 ${\sim}90$도 정도의 예리한 DNA bending을 유도한다. 그러나 이전의 논문[5]에 의하면 돌연변이 CRP:cGMP 복합체는 돌연변이 CRP:cAMP 복합체보다 아크릴아미드 겔에서 상대적으로 빠른 이동속도를 보였다. CRP와 cyclic nucleotide 존재하에서 DNA의 구조 변화를 알아보기 위하여 6가지 준비된 DNA조각들을 사용하여 몰 고리화 인자(molar cyclization factor)[13]를 측정하였다. 이들 자료를 사용하여 nonlinear regression analysis를 통하여 cGMP 존재하에서 돌연변이 CRP는 DNA bending을 형성하지 않으나 CAMP 존재하에서 나선 꼬임과 같은 DNA 구조 변화없이 DNA bending을 형성한다.

• 과학교육연구지
• /
• 제35권2호
• /
• pp.221-229
• /
• 2011

여학생들의 이공계 분야의 진출을 돕기 위해 다각적으로 많은 여학생을 위한 과학교육 프로그램의 개발 및 연구가 요구되고 있다. 여학생 친화 과학교육 프로그램 개발을 위해 방학 중에 실시되는 과학캠프와 정규 학기 중에 학교를 방문하여 여학생들에게 멀티미디어 교육매체의 하나로서 공상과학 영화를 보여주고 과학적인 관심도에 대한 설문지 검사를 통하여 여학생의 과학적 관심도 변화를 측정 분석하는 선행 연구적 성격의 연구 결과를 제시한다. 일반중학교 남녀학생에게 같은 설문을 하여 남학생과 여학생간의 반응차이를 비교하여 보았으며, 분석한 결과에 따르면 영화매체를 이용한 프로그램은 남학생 보다 여학생들에게 과학적인 관심과 학습 유도의 효과가 대체로 조금 더 크게 나타남을 알 수 있었다. 나아가 영화내용의 과학적 지식에 대한 사전교육과 사후 토론 및 검토 등이 영화매체를 이용한 여학생들의 과학 친화 프로그램의 효율성을 높이는데 효과적일 수 있음이 보여졌다.