• Journal of Microbiology and Biotechnology
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• 제28권5호
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• pp.809-815
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• 2018

Influenza, which is a highly contagious disease caused by the influenza A virus, continues to be a major health concern worldwide. Although the accurate and early diagnosis of influenza virus infection is important for controlling the spread of this disease and rapidly initiating antiviral therapy, the current influenza diagnostic kits are limited by their low sensitivity. In this study, we developed several new influenza nucleoprotein (NP)-specific monoclonal antibodies (mAbs) and compared their sensitivity and specificity of those with commercially available anti-NP mAbs. Three mAbs, designated M24.11, M34.3, and M34.33, exhibited higher reactivities to recombinant NPs and A/Puerto Rico/8/1934 (H1N1) viral lysates compared with the commercial mAbs, as assessed using enzyme-linked immunosorbent assays. M34.3 and M34.33 showed higher reactivities with A/California/04/09 (pandemic H1N1) and A/Philippines/2/82 (H3N2) viral lysates than the commercial mAbs. In contrast, M24.11 had marked reactivity with H3N2 but not with pandemic H1N1. Immunofluorescent confocal microscopy showed that the three mAbs effectively detected the presence of influenza virus in lung tissues of mice infected with A/Puerto Rico/8/1934. These results indicate that the newly developed M34.3 and M34.33 mAbs could be useful for the development of influenza diagnostics.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.230-230
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• 2017

About 200 rice varieties for irrigated and rainfed lowland ecosystems were released in the Philippines, which were bred for improving yield under favorable conditions. Root plasticity plays key roles in maintaining crop productivity under abiotic stressed conditions. We hypothesized that some of these varieties possess root plasticity traits in response to water stressed conditions. This study aimed to evaluate the root system development and dry matter production of 14 randomly selected rice varieties (6 irrigated lowland and 9 rainfed varieties) under progressive drought (PDR) and soil moisture fluctuations (SMF) stress conditions. Two experiments were done in rootbox and line source sprinkler systems (LSS). Each of the varieties was subjected to well-watered (WW), PDR and SMF conditions during vegetative stage in rootbox system while the same genotypes were subjected to different intensities of drought stress under LSS. Under rootbox system, PDR and SMF significantly reduced shoot dry matter production in all varieties relative to their WW controls. Among varieties, NSIC Rc238 (irrigated lowland) showed the least reduction in shoot dry weight (SDW) in both PDR (by 11.8%) and SMF (by 26.9%) conditions. Less reductions in SDW of NSICRc238 were partially attributed to the promotion of L-type lateral roots, thus increasing total lateral root length by 24.2% and 30.7% under PDR and SMF, respectively. In LSS, SDW of NSIC Rc238 under mild drought stress (16-21% soil moisture content (SMC) had 31.8% reduction relative to its WW control (${\geq}22%SMC$) and had lower sensitivity drought index. Compared with the IR64 susceptible check and NSIC Rc9 tolerant check, NSIC Rc238 had higher SDW by 90.8% and 38.6%, respectively. Furthermore, no rainfed lowland varieties included in the experiment performed well under different water stress treatments. The results implied that some other irrigated lowland rice varieties may also possess drought dehydration avoidance root plasticity traits under water-stressed growing environments.

• 한국작물학회지
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• 제44권1호
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• pp.32-37
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• 1999

The experiment was conducted to investigate emergence response of lowland weeds at different soil moisture contents, burying depths and upon changes in soil moisture. Rice germination was over 50% at all burying depths under aerobic condition, but the emergence rate of the soil surface placed seeds in saturated and flooded conditions decreased by 19% and 29%, respectively, as compared with that of aerobic condition. Rice seeds at burying depth of over 3 cm did not emerge at all. The emergence rate of Echinochloa crus­galli (L.) Beauv. in aerobic condition was lower than 30%, but the emergence pattern of E. crus galli (L.) Beauv. at different soil moisture contents and seeding depths was similar to that of rice. Emergence behavior of lschaemum rugosum Salisb., Ludwigia octovalvis (Jacq.) Raven and Sphenoclea zeylanica Gaertn. which are dominant lowland weed species in the Philippines also differed depending on soil moisture conditions and burying depths. lschaemum rugosum Salisb. emerged at all burying depths under aerobic condition, whereasLudwigia octovalvis (Jacq.) Raven emerged only at 0 cm deep under saturated and aerobic condition and Sphenoclea zeylanica Gaertn. at 0 cm deep under flooding condition. Weed seeds planted at 1, 3, and 5 cm deep in continuous flooded and saturated condition did not emerge at all, but upon a change of soil moisture condition from saturated to drainage (S$\rightarrow$D) and flooded to drainage (F$\rightarrow$D), grass weeds began to germinate again and the average emergence rate in S$\rightarrow$D and F$\rightarrow$D were 26% and 5% forE. crus­galIi (L.) Beauv., 9% and 8% forI. rugosum SaIisb., respectively. Weed seeds buried in soil in the pot showed great emergence at S$\rightarrow$D but did not emerge under continuous flooded condition. The diversity index accounting for dominance degree and occurrence aspect of weed, was the lowest at F$\rightarrow$D.

• Asian-Australasian Journal of Animal Sciences
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• 제26권11호
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• pp.1651-1658
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• 2013

Nitrate contamination in ground and surface water is an increasingly serious environmental problem and only a few bacterial strains have been identified that have the ability to remove nitrogen pollutants from wastewater under thermophilic conditions. We therefore isolated thermophilic facultative bacterial strains from wood chips that had been composted with swine manure under aerated high temperature conditions so as to identify strains with denitrifying ability. Nine different colonies were screened and 3 long rod-shaped bacterial strains designated as SG-01, SG-02, and SG-03 were selected. The strain SG-01 could be differentiated from SG-02 and SG-03 on the basis of the method that it used for sugar utilization. The 16S rRNA genes of this strain also had high sequence similarity with Geobacillus thermodenitrificans $465^T$ (99.6%). The optimal growth temperatures ($55^{\circ}C$), pH values (pH 7.0), and NaCl concentrations (1%) required for the growth of strain SG-01 were established. This strain reduced 1.18 mM nitrate and 1.45 mM nitrite in LB broth after 48 h of incubation. These results suggest that the G. thermodenitrificans SG-01 strain may be useful in the removal of nitrates and nitrites from wastewater generated as a result of livestock farming.

• 한국치위생학회지
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• 제12권2호
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• pp.409-417
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• 2012

Objectives : The purpose of this study is to figure out and establish the basic data that can help improve management of oral healthcare for the international marriage immigrant women. Methods : In this sense, we surveyed questions on 237 women out of 1,300 immigrant women, who have participated in the program by multi-cultural household supporting center, in a fashion of face-to-face investigation and on-the-spot direct cavity inspection simultaneously from May 1 to October 31, 2010. Collected data were electro-statistically computerized under SPSS 17.0 program and analyzed with frequency analysis, recurrence analysis and logistic regression analysis respectively. Results : The number of filled teeth got lower when they had lower number of dental visits. Women from the Philippines had the highest number of defect teeth, and the number of fixed prosthetic appliance became less when they had less number of dental visits. When they had no experience of oral health education, they had less fixed prosthetic appliances. There was higher percentage of periodontal disease when they did not have dental visit compared with the dental visit cases. Conclusions : Preparation of a policy for east Asian immigrant women for them to visit dentist comfortably and expansion of systematic oral health education are highly recommended.

• Journal of Applied Biological Chemistry
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• 제61권2호
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• pp.135-139
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• 2018

The inhibition of aldose reductase (AR) has been shown to prevent the progression of the many complications associated with diabetic hyperglycemia. Several compounds purified from various plant sources have exhibited potent inhibition against AR. In this study, the inhibitory effects of the methanol extracts of the flowers of Synurus excelsus and Weigela subsessilis on AR were determined in vitro. Scopolin and scopoletin are coumarins isolated from the flowers of S. excelsus and W. subsessilis; and quercetin is a known AR inhibitor present in many flowers. To determine and quantify their presence in both plants, HPLC-UV analysis of all three compounds was performed. S. excelsus and W. subsessilis showed potent inhibition against AR having $IC_{50}$ values of 0.17 and $0.14{\mu}g/mL$, respectively. The concentration of scopolin in S. excelsus and W. subsessilis were 34.71 and 174.14 mg/g extract, respectively. Scopoletin was detected in S. excelsus at 3.41 mg/g extract, whereas quercetin was not detected in both plants. This study shows that S. excelsus and W. subsessilis exhibited promising AR inhibitory effects and are both sources of coumarins.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2015년도 춘계학술대회
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• pp.67-70
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• 2015

정보통신(ICT)산업의 빠른 진화 속도에 따라, ICT 산업을 뒷받침할 인력양성(HRD)의 필요성이 꾸준히 대두 되고 있다. 특히 우리나라의 경우, 유엔 전자정부발전지수 연속1위, ITU의 ICT개발지수 2위등 우수한 공공인프라를 유지하고 있고, 학계에서는 ICT전공자를 꾸준히 배출함에도 불구하고, 해당 산업계에서의 만족도는 많은 차이(Gap)가 있어서 산업계 스스로 재교육이나 이에 따른 사회적 비용이 부가적으로 존재하고 있는 실정이다. 이러한 현상은 다른 나라에서도 동일한 사회적 현상이다. 이에, ICT산업 종사자 및 소프트웨어 개발자가 비즈니스를 이해하고, 요구사항에 따른 과제를 해결하여 산업계 진입 후, 특정 직무나 직위에 상관없이 비즈니스 요구에 따른 문제를 해결하고 업무를 성공적으로 수행하는데 요구되는 기본적인 핵심적 지식, 스킬, 태도의 종합적인 능력수준을 진단하고 평가하는 제도와 도구의 필요성이 대두되어 왔다, 이에 본 논문에서는 IT공통역량 강화를 도모하는 교수-학습-실무현장을 위한 제도, 개념 및 구조, 정책과 영향에 따라 개발된 제도적 시스템을 소개하고 결과를 평가한다. 이 시스템은 ITU-ASP 주관 ICTD-USO포럼 기간 중 초청 소개 되었고, 주요 ASEAN국가들의 관심과 지원요청을 받았다.

• 환경위생공학
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• 제21권3호
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• pp.9-14
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• 2006

From March 1, 2005 to August 31, 2006 mineral water wells were selected for 13 areas in Southeast Asia (Indonesia, Philippines, Thailand, Singapore) and 20 areas in Northeast Asia (Korea, North Korea, China, Japan) and the study on their mineral water quality was analyzed. - Mineral water quality was the best in Korea. Mineral spring waters in some area of China and North Korea and in the whole area of the Southeast Asia were of poor quality. - The hardness of mineral water was the low in Korea $(10{\sim}47mg/L\;as\;CaCO_3)$, Japan $(4{\sim}66mg/L\;as\; CaCO_3)$, Geumgang-san North Korea Area $(4mg/L\;as\;CaCO_3)$. Mineral spring water in Thailand, Indonesia $(1{\sim}97.5mg/L\;as\;CaCO_3)$ and in the other area $(120{\sim}1205mg/L\;as\;CaCO_3)$ were high degree of hardness. - pH value in the mineral water of Southeast Asia $(pH\;6.7{\sim}8.2)$ and Northeast Asia $(pH\;5.9{\sim}7.9)$ was up to WHO standard $(pH\;6.5{\sim}8.5)$. Fluorine of negative ion was found in 10 mineral waters: Indonesian mineral water 'ATARTN'(0.02mg/L), Thailand mineral water 'SIAM' (0.6mg/L), 'MASAFI' (0.02mg/L). Korean mineral water 'SAEMMULNARA' (1.1mg/L), 'SANSU'(0.6mg/L), 'ICIS'(0.3mg/L), 'DONGWON SAEM-MUL'(0.03mg/L), 'PYEONGCHANG' (0.6mg/L), North Korean mineral water 'KUMGANGSAN'(0.1mg/L), Japanese mineral water 'CRYSTAL GEYSER'(0.55mg/L). However Fluorine in the other 23 mineral waters were not detectable.

• Journal of Microbiology
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• 제45권3호
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• pp.219-226
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• 2007

Insertion sequence IS1112 is a repetitive element with a relatively high number of copies in Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight of rice (Oryza sativa L.). Three new loci of IS1112 were identified in seven Chinese strains of Xoo using a single oligonucleotide primer J3; 5'-GCTCA GGTCAGGTGGCCTGG-3' by insertion-sequence-based polymerase chain reaction (IS-PCR). Among the three new loci of IS1112, two were located in the open-reading frame region of genes fhuA and cirA, which encode TonB-dependent receptors, and the third in ISXo2, another type of insertion sequence in Xoo genome. Three variants of IS1112 were identified in those three loci based on their sequence similarities: two were identical to IS1112a and IS1112b, reported in strain PXO86 from the Philippines, while the third was a new member of IS1112, defined as IS1112d. Inserting IS1112 in gene fhuA caused three bases, GGT, to be duplicated at the target site, but inserting it in gene cirA did not cause any duplication in the target site. The diversity of IS1112 sequence and insertion loci in Xoo genome and their potential effects are discussed.

• Journal of Microbiology and Biotechnology
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• 제33권4호
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• pp.441-448
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• 2023

Brucellosis is a contagious zoonotic disease that infects millions of people annually with hundreds of millions more being exposed. It is caused by Brucella, a highly infectious bacterial species capable of infecting humans with an estimated dose of 10-100 organisms. Sirtuin 1 (SIRT1) has been reported to contribute to prevention of viral diseases as well as a chronic infection caused by Mycobacterium bovis. Here, we investigated the role of SIRT1 in the establishment of Brucella abortus infection in both in vitro and in vivo systems using the reported SIRT1 activators resveratrol (RES), piceatannol (PIC), and ginsenoside Rg3 (Rg3). In RAW264.7 cells, SIRT1 activators did not alter the adherence of Brucella or Salmonella Typhimurium. However, reduced uptake of Brucella was observed in cells treated with PIC and Rg3, and survival of Brucella within the cells was only observed to decrease in cells that were treated with Rg3, while PIC treatment reduced the intracellular survival of Salmonella. SIRT1 treatment in mice via oral route resulted in augmented Brucella resistance for PIC and Rg3, but not RES. PIC treatment favors Th2 immune response despite reduced serum pro-inflammatory cytokine production, while Rg3-treated mice displayed high IL-12 and IFN-γ serum production. Overall, our findings encourage further investigation into the complete mechanisms of action of the different SIRT1 activators used as well as their potential benefit as an effective alternative approach against intracellular and extracellular pathogens.