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Comparison of Vitrification and Slow Freezing for the Cryopreservation of Mouse Pronuclear Stage Embryos (생쥐 전핵기 배아 냉동보존에서 완만동결과 유리화동결의 비교)

  • Kim, Mi-Young;Lee, Yu-Il
    • Clinical and Experimental Reproductive Medicine
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    • v.34 no.2
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    • pp.117-124
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    • 2007
  • Objective: The aim of this study was to compare the efficacy of slow freezing with vitrification method for cryopreservation of mouse pronuclear stage embryos. Methods: Mouse pronuclear embryos obtained from superovulated mice and classified into 2 groups of slow freezing and vitrification. Slow freezing solution consisted of 1.5 M PROH, 0.1 M sucrose, while vitrification solution consisted of 40% ethylene glycol, 18% Ficoll and 0.5 M sucrose diluted in Dulbecco's phosphate-buffered saline supplemented with 10% SSS. Recovery and survival rates after thawing and development rates to hatching balstocyst stage were compared between two groups. Results: After freezing and thawing, recovery rate of slow freezing group was 93.8%, whereas vitrification group was 66.5% (p<0.01). Survival rate of recovered embryos were similar between two groups as 83.2% in slow freezing and 87.6% in vitrification. Embryo development rates to 2-cell stage after 24 hrs (77.0% vs 59.1%), 4-cell after 48 hrs (72.6% vs 53.3%), blastocyst after 96 hrs (53.1% vs 40.1%) of thawing were significantly higher in vitrification group than those of slow freezing group, respectively. Conclusion: The vitrification method may provide better developmental competence of frozen-thawed embryos than that of slow freezing method for cryopreservation of mouse pronuclear stage embryos.

Availability of Land Surface Temperature Using Landsat 8 OLI/TIRS Science Products (Landsat 8 OLI/TIRS Science Product를 활용한 지표면 온도 유용성 평가)

  • Park, SeongWook;Kim, MinSik
    • Korean Journal of Remote Sensing
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    • v.37 no.3
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    • pp.463-473
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    • 2021
  • Recently, United States Geological Survey (USGS) distributed Landsat 8 Collection 2 Level 2 Science Product (L2SP). This paper aims to derive land surface temperature from L2SP and to validate it. Validation is made by comparing the land surface temperature with the one calculated from Landsat 8 Collection 1 Level 1 Terrain Precision (L1TP) and the one from Automated Synoptic Observing System (ASOS). L2SP is calculated from Landsat 8 Collection 2 Level 1 data and it provides land surface temperature to users without processing surface reflectance data. Landsat 8 data from 2018 to 2020 is collected and ground sensor data from eight sites of ASOS are used to evaluate L2SP land surface temperature data. To compare ground sensor data with remotely sensed data, 3×3 grid area data near ASOS station is used. As a result of analysis with ASOS data, L2SP and L1TP land surface temperature shows Pearson correlation coefficient of 0.971 and 0.964, respectively. RMSE (Root Mean Square Error) of two results with ASOS data is 4.029℃, 5.247℃ respectively. This result suggests that L2SP data is more adequate to acquire land surface temperature than L1TP. If seasonal difference and geometric features such as slope are considered, the result would improve.

Comparison of Topographic Surveying Results using a Fixed-wing and a Popular Rotary-wing Unmanned Aerial Vehicle (Drone) (고정익 무인항공기(드론)와 보급형 회전익 무인항공기를 이용한 지형측량 결과의 비교)

  • Lee, Sungjae;Choi, Yosoon
    • Tunnel and Underground Space
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    • v.26 no.1
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    • pp.24-31
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    • 2016
  • Recently, many studies have been conducted to use fixed-wing and rotary-wing unmanned aerial vehicles (UAVs, Drones) for topographic surveying in open-pit mines. Because the fixed-wing and rotary-wing UAVs have different characteristics such as flight height, speed, time and performance of mounted cameras, their results of topographic surveying at a same site need to be compared. This study selected a construction site in Yangsan-si, Gyeongsangnam-do, Korea as a study area and compared the topographic surveying results from a fixed-wing UAV (SenseFly eBee) and a popular rotary-wing UAV (DJI Phantom2 Vision+). As results of data processing for aerial photos taken from eBee and Phantom2 Vision+, orthomosaic images and digital surface models with about 4 cm grid spacing could be generated. Comparisons of the X, Y, Z-coordinates of 7 ground control points measured by differential global positioning system and those determined by eBee and Phantom2 Vision+ revealed that the root mean squared errors of X, Y, Z-coordinates were around 10 cm, respectively.

Development of Change Detection Technique Using Time Seriate Remotely Sensed Satellite Images with User Friendly GIS Interface (사용자 중심적 GIS 인터페이스를 이용한 시계열적 원격탐사 영상의 변화탐지 기법의 개발)

  • 양인태;한성만;윤희천;김흥규
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.22 no.2
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    • pp.151-159
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    • 2004
  • The diversity, expansion of human activity and rapid urbanization make modem society to faced with problems like damage of nature and drain of natural resources. Under these circumstances rapid and accurate change detection techniques, which can detect wide range utilization changes, are needed for efficient management and utilization plan of national territory. In this study to perform change detection from remote sensing images, space analysis technique contained in Geographic Information System is applied. And from this technique, the software. that can execute new change detection algorithm, query, inquiry and analysis, is produced. This software is on the basis of graphic user interface and has many functions such as format conversion, grid calculation, statistical processing, display and reference. In this study, simultaneously change detection for multi-temporal satellite images can be performed and integrated one change image about four different periods was produced. Further more software user can acquire land cover change information for an specific area through querying and questioning about yearly changes. Finally making of every application module for change detection into one window based visual basic program, can be produced user convenience and automatic performances.

Flood Runoff Analysis using Radar Rainfall and Vflo Model for Namgang Dam Watershed (레이더강우와 Vflo모형을 이용한 남강댐유역 홍수유출해석)

  • Park, Jin-Hyeog;Kang, Boo-Sik;Lee, Geun-Sang;Lee, Eul-Rae
    • Journal of the Korean Association of Geographic Information Studies
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    • v.10 no.3
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    • pp.13-21
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    • 2007
  • Recently, very short-term rainfall forecast using radar is required for regional flash flood according to climate change. This research is to evaluate the feasibility of GIS based distributed model using radar rainfall which can express temporal and spatial distribution in actual dam watershed during flood runoff period. Vflo model which was developed Oklahoma university was used as physical based distributed model, and Namgang dam watershed ($2,293km^2$) was applied as study site. Distributed rainfall according to grid resolution was generated by using K-RainVieux, preprocess program of radar rainfall, from JIN radar. Also, GIS hydrological parameters were extracted from basic GIS data such as DEM, land cover and soil map, and used as input data of distributed model(Vflo). Results of this research can provide a base for building of real-time short-term rainfall runoff forecast system according to flash flood in near future.

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Planning and Dosimetric Study of Volumetric Modulated Arc Based Hypofractionated Stereotactic Radiotherapy for Acoustic Schwannoma - 6MV Flattening Filter Free Photon Beam

  • Swamy, Shanmugam Thirumalai;Radha, Chandrasekaran Anu;Arun, Gandhi;Kathirvel, Murugesan;Subramanian, Sai
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.12
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    • pp.5019-5024
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    • 2015
  • Background: The purpose of this study was to assess the dosimetric and clinical feasibility of volumetric modulated arc based hypofractionated stereotactic radiotherapy (RapidArc) treatment for large acoustic schwannoma (AS >10cc). Materials and Methods: Ten AS patients were immobilized using BrainLab mask. They were subject to multimodality imaging (magnetic resonance and computed tomography) to contour target and organs at risk (brainstem and cochlea). Volumetric modulated arc therapy (VMAT) based stereotactic plans were optimized in Eclipse (V11) treatment planning system (TPS) using progressive resolution optimizer-III and final dose calculations were performed using analytical anisotropic algorithm with 1.5 mm grid resolution. All AS presented in this study were treated with VMAT based HSRT to a total dose of 25Gy in 5 fractions (5fractions/week). VMAT plan contains 2-4 non-coplanar arcs. Treatment planning was performed to achieve at least 99% of PTV volume (D99) receives 100% of prescription dose (25Gy), while dose to OAR's were kept below the tolerance limits. Dose-volume histograms (DVH) were analyzed to assess plan quality. Treatments were delivered using upgraded 6 MV un-flattened photon beam (FFF) from Clinac-iX machine. Extensive pretreatment quality assurance measurements were carried out to report on quality of delivery. Point dosimetry was performed using three different detectors, which includes CC13 ion-chamber, Exradin A14 ion-chamber and Exradin W1 plastic scintillator detector (PSD) which have measuring volume of $0.13cm^3$, $0.009cm^3$ and $0.002cm^3$ respectively. Results: Average PTV volume of AS was 11.3cc (${\pm}4.8$), and located in eloquent areas. VMAT plans provided complete PTV coverage with average conformity index of 1.06 (${\pm}0.05$). OAR's dose were kept below tolerance limit recommend by American Association of Physicist in Medicine task group-101(brainstem $V_{0.5cc}$ < 23Gy, cochlea maximum < 25Gy and Optic pathway <25Gy). PSD resulted in superior dosimetric accuracy compared with other two detectors (p=0.021 for PSD.

The Evaluation of Reliability for Exam Distance of Visual Acuity (시력검사거리에 따른 원거리 시력검사 신뢰성 평가)

  • Chun, Young-Yun;Choi, Hyun-Soo;Park, Seong-Jong;Lee, Seok-Ju
    • Journal of Korean Ophthalmic Optics Society
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    • v.19 no.1
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    • pp.17-22
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    • 2014
  • Purpose: We aimed to evaluate reliability of eye exam for visual acuity as a function of distance. Methods: There were 39 patients (78 eyes) who had visual acuity 1.0 or more at 5 meters. We measured refractive power of patients at each distances, 5 meters, 4 meters and 3 meters. Automatic chart (LCD-700, Hyeseong Optic. Co., Korea) used for visual acuity, skiascope (Beta 200, Heine, Germany) and auto refractometer (RK-5, Canon, Japan) used as for objective refraction. Accommodation was examined by minus lens addition methods, and Accommodative lag was examined by grid chart for reading distance. Results: Being compared to 3 meter test, Amount of corrected spherical refractive power decreased by $0.10{\pm}0.38$ D, astigmatism decreased by $0.05{\pm}0.10$ D, and axis of astigmatism rotated toward to temporal by $2.64{\pm}18.75$ degrees for right eyes, by $11.43{\pm}48.55$ degrees for left eyes in case of 5 meter test. Changes of corrected refraction and astigmatism were slightly correlated (r=-0.31, r=-0.29). Conclusions: Because corrected refraction power and amount of astigmatism decreased and axis of astigmatism tends to turn the temporal direction according to exam distance, examination distance of visual acuity should improved as to 5 meters.

Forecast study for active factor of V2B(Vehicle to Building) operation zero energy building using monte carlo method (몬테카를로방법을 이용한 V2B(Vehicle to Building) 운용 제로에너지빌딩의 액티브 요소 예측 연구)

  • Kim, Youngil;Kim, Insoo
    • Journal of Energy Engineering
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    • v.26 no.4
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    • pp.29-34
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    • 2017
  • Factors of Zero-Energy Building are divided into active and passive factor. Passive factor means insulation, heat bridge of building like insulation, windows and doors, awning, outside etc. and active factor means energy output and efficiency coefficient. Energy output of active factor is achieved by new generating energy. This study anticipated how many effects will be produced when not new generating energy but Vehicle to Building; V2B, bi-directional charging and discharging technology, is applied to Zero-Energy Building. In new generating energy, power generation will be anticipated by geography and climate, but in V2B, several input variable like user's discharging intention and number of usable charger etc. should be considered. We can check how much V2B contribute to the Zero-Energy Building by anticipated results, and that results should be anticipated by using probabilitic method because there is few statistical data. This study anticipate change of charging and discharging pattern, based by Demand Response slot, by using monte carlo method among the probabilitic methods.

Quantification of Environmental Characteristics on Citrus Production Area of Jeju Island in Korea (제주도 감귤 재배지역에 대한 환경특성의 정량화)

  • Moon, Kyung Hwan;Son, In-Chang;Song, Eun Young;Oh, SoonJa;Park, Kyo Sun;Hyun, Hae-Nam
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.17 no.1
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    • pp.69-74
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    • 2015
  • To analyze quantitatively environmental characteristics of cultivation area of citrus, Satsuma mandarin (Citrus unshiu Marc.), we made digital maps of environmental elements such as topography and climate. Elevation, degree of slope, and slope aspect were selected as elements of topological environment, and the annual mean air temperature, annual total precipitation, mean air temperature on January, extreme value of daily minimum air temperature, and the number of days below $-5^{\circ}C$ were selected as elements of climatic environments. The grid values of 8 environmental elements were extracted by shape of citrus farm area and analyzed distribution patterns. We can determine 3 agroclimatic criteria for growing Satsuma mandarin as over $14.5^{\circ}C$ of annual mean air temperature, over $-10.0^{\circ}C$ of extreme value of daily minimum air temperature, and less 5 days of below $-5^{\circ}C$ of daily minimum air temperature.

Analysis of Relative Breakage Hazard Rate of Water Mains Using the Proportional Hazards Model (비례위험모형을 이용한 상수관로의 상대적 파손위험율 분석)

  • Park, Su-Wan;Kim, Jung-Wook;Im, Gwang-Chae;Lee, Hyeong-Seok
    • Proceedings of the Korea Water Resources Association Conference
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    • 2008.05a
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    • pp.490-494
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    • 2008
  • 본 연구에서는 상수도 배수관로의 내 외부적 특성에 따라 개별관로를 정의하는 방법을 연구대상 지역의 배수관로 파손 데이터베이스에 적용하여 비례위험모형을 구축하였다. 연구에 사용된 자료는 연구대상지역의 배수관로의 제원 및 파손시기를 포함하는 관로 파손데이터베이스, 관로매설지역의 급수인구 및 수압범위에 관한 자료를 포함하는 GRID 데이터베이스와 관로매설지역의 토지개발 정도에 관한 자료를 포함한다. 이러한 자료를 이용하여 관로를 순차적 파손경험에 따라 7개의 생존시간군(STG I $\sim$ VII)으로 구분하고 각 생존시간군에 대한 비례위험모형(Model I $\sim$ VII)을 구축하였다. 이러한 모형을 이용하여 관로의 파손횟수가 증가하는 동안 파손에 영향을 미치는 인자의 변화와 그 효과를 파악하였으며, 또한 추정된 공변수의 위험비율을 분석함으로써 관로의 제원 혹은 매설환경, 급수인구 등에 따른 위험률의 상대적인 변화를 분석하였다. 또한 비례 위험모형의 구축과정에서 관로의 파손에 영향을 미치는 공변수의 비례성 가정을 검토하여 시간종속형 공변수를 모형화하였으며, 모형의 이탈잔차(deviance residual)를 분석하여 모형의 적합성을 검토하였다. 본 연구에서 구축된 비례위험모형에 대해 Shoenfeld 잔차를 이용한 스코어 잔차의 변화(score process)를 검토한 결과, Model I 과 Model II 에 대해서는 공변수의 시간종속 효과가 발견되었다. Model I에 대해서는 관로재질과 급수인구의 영향이 시간에 따라 변하며 Model II에서는 급수인구의 영향만이 시간에 따라 변하는 것으로 나타났다. 한편 Model III $\sim$ Model VII 들에 대해서는 공변수의 영향이 시간에 따라 변하지 않는 것으로 나타났다. 각 생존시간군에 대해 관로재질, 토지개발정도, 관로길이 및 급수인구의 변화가 관로의 상대적 누수위험률에 미치는 영향을 상대위험률의 95% 신뢰구간을 고려하여 정량적으로 산정하였고, 시간 종속형 공변수로 모형화된 공변수는 시간에 따른 공변수 영향의 변화를 분석하였다. 순차적 파손사건에 대한 비례위험모형의 구축 결과 생존시간군(STG) I의 기저위험률은 매설 후 대략 450개월까지는 파손 위험률이 '0'에 가까우나 그 이후로 급격히 증가하다가 매설 후 약 700개월에 이르러서는 약간 감소하고 약 850개월 이후에는 다시 급격히 증가한다. STG II의 기저위험률은 첫 번째 파손 후 약 300개월이 되면 위험률이 급격히 증가하는 것으로 나타났다. STG III $\sim$ STG VII의 기저위험률은 이차함수의 형태를 띄며, 특히 STG V, STG VI 및 STG VII의 기저위험률은 욕조형 곡선(bathtub curve)의 형태를 가진다. 각 생존시간군의 기저생존함수의 생존확률 '0.5'에 해당하는 기저중간생존시간에 대한 분석으로부터 파손횟수가 많아질수록 순차적 파손사건 사이의 경과시간은 감소하는 것으로 나타났다. 이러한 기저생존시간에 대한 경향은 관로의 파손횟수가 많아질수록 관로의 일반적은 내구성은 감소하기 때문인 것으로 분석된다.

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