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A study of mesenchymal stem cell proliferation and surface characteristics of the titanium discs coated with MS275/PLGA by an electrospray (Electrospray법을 이용한 MS275/PLGA submicron 코팅 티타늄에서의 표면변화와 간엽줄기세포증식에 관한 연구)

  • Yoo, Soo-Yeon;Kim, Seong-Kyun;Heo, Seong-Joo;Koak, Jai-Young;Lee, Joo-Hee;Park, Yoon-Kyung;Kim, Ena
    • The Journal of Korean Academy of Prosthodontics
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    • v.50 no.4
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    • pp.285-291
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    • 2012
  • Purpose: This study was conducted to identify the surface characteristics of titanium discs coated with MS275/PLGA by electrospray and which is effective to mesenchymal stem cell proliferation. Materials and methods: We used anodized surface coated with PLGA as a control group and anodized surface coated with MS275 $0.5{\mu}M$, $1{\mu}M$, $1.5{\mu}M$ as test groups. To examine that the coating particles are nanometer sized, FE-SEM was used and AFM was utilized to determine the difference of coating surface roughness. We checked the mesenchymal stem cell proliferation by using MTT assay on $1^{st}$, $4^{th}$, $7^{th}$ days. Results: There was no significant difference between control groups and test groups in AFM results (P>.05). In MTT assay results, mesenchymal stem cell proliferation was increased with time, at $7^{th}$ day, cell viability on discs coated with $1.5{\mu}M$ MS275 was significantly higher than control group (P<.05). As SEM showed, the number of cells on all discs was increased and the morphology of cell attachment was also wider and closer with time. Conclusion: Titanium surface coated with MS275/PLGA showed significantly higher cell proliferation and the more density of MS275 was dispersed on titanium discs, the faster cells grew.

Dose Distribution and Design of Dynamic Wedge Filter for 3D Conformal Radiotherapy (방사선 입체조형치료를 위한 동적쐐기여과판의 고안과 조직내 선량분포 특성)

  • 추성실
    • Progress in Medical Physics
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    • v.9 no.2
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    • pp.77-88
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    • 1998
  • Wedge shaped isodoses are desired in a number of clinical situations. Hard wedge filters have provided nominal angled isodoses with dosimetric consequences of beam hardening, increased peripheral dosing, nonidealized gradients at deep depths along with the practical consequendes of filter handling and placement problems. Dynamic wedging uses a combination of a moving collimator and changing monitor dose to achieve angled isodoses. The segmented treatment tables(STT) that monitor unit setting by every distance of moving collimator, was induced by numerical formular. The characteristics of dynamic wedge by STT compared with real dosimetry. Methods and Materials : The accelerator CLINAC 2100C/D at Yonsei Cancer Center has two photon energies (6MV and 10MV), currently with dynamic wedge angles of 15$^{\circ}$, 30$^{\circ}$, 45$^{\circ}$ and 60$^{\circ}$. The segmented treatment tables(STT) that drive the collimator in concert with a changing monitor unit are unique for field sizes ranging from 4.0cm to 20.0cm in 0.5cm steps. Transmission wedge factors were measured for each STT with an standard ion chamber. Isodose profiles, isodose curves, percentage depth dose for dynamic wedge filters were measured with film dosimetry. Dynamic wedge angle by STT was well coincident with film dosimetry. Percent depth doses were found to be closer to open field but more shallow than hard wedge filter. The wedge transmission factor were decreased by increased the wedge angle and more higher than hard wedge filters. Dynamic wedging probided more consistent gradients across the field compared with hard wedge filters. Dynamic wedging has practical and dosimetric advantages over hard filters for rapid setup and keeping from table collisions. Dynamic wedge filters are positive replacement for hard filters and introduction of dynamic conformal radiotherapy and intensity modulation radiotherapy in a future.

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Effect of Heat-Moisture Treatment of Domestic Rice Flours Containing Different Amylose Contents on Rice Noodle Quality (아밀로오스 함량이 다른 국내산 쌀가루의 수분-열처리가 쌀국수 품질에 미치는 영향)

  • Seo, Hye-In;Ryu, Bog-Mi;Kim, Chang-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.11
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    • pp.1597-1603
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    • 2011
  • The influence of heat-moisture treatment (HMT) and substitution of rice flour containing different amylose contents on the quality characteristics of rice noodles was investigated. HMT was applied to rice flours with 21% moisture content at 100 and 105$^{\circ}C$ for 30 min. Three rice cultivars were used, including high amylose of Goami (GM) and intermediate amylose of Choochung (CC) as domestic rice flours and imported rice of Taeguk (TG) as a control. HMT and substitution of rice flour with different amylose contents affected the cooking and texture quality of rice noodles. When rice noodles were made of intermediate amylose rice flour with HMT, cooking properties improved with decreased cooking loss and cooking water turbidity and thus were closer to those of control. Especially, the hardness, adhesiveness, tensile strength, and darkness of rice noodles notably increased when HMT rice flour was used. Based on the results of quantitative descriptive analysis for selected rice noodles, the noodles made of HMT CC at 105$^{\circ}C$ (CC105) had high scores for resilience and adhesiveness and low scores for hardness compared with imported commercial rice noodles and other experimental noodles such as TG, HMT GM100, TG+CC, and TG+CC105. In conclusion, rice noodles were made of composite flours containing high amylose and intermediate amylose contents or HMT intermediate amylose content rice flour.

Screening of Antifungal Activity on the Coastal Plants 5 Species (해안식물 5종에 대한 항균활성 탐색)

  • Kwon, Nan-Hee;Kim, Tae-Keun;Park, Sung-Jun;Kim, Hyoun-Chol;Song, Chang-Khil
    • Korean Journal of Organic Agriculture
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    • v.24 no.3
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    • pp.465-484
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    • 2016
  • This study evaluated the antifungal activity of varying concentrations of water-soluble extracts from native plants (Vitex rotundifolia, Tetragonia tetragonoides, Artemisia capillaris, Hibiscus hamabo and Ficus carica) against Stemphylium vesicarium, Penicillium italicum, Sclerotinia sclerotiorum, Pythium ultimum, Botrytis cinerea, Rhizoctonia solani and Colletotrichum gloeosporioides. Mycelium growth of pathogenic bacteria generally decreased in a concentration-dependent manner following treatment with the water extracts from donor plants. Closer analyses indicate varying inhibitory capacities depending on the type of donor plant and pathogenic bacteria. Specifically, mycelium growth of S. vesicarium varied depending on the concentration of the water extracts from T. tetragonoides (r = -0.857, p<0.01) and A. capillarys (r = -0.868, p<0.01). Also, P. italicum and V. rotundifolia (r = -0.833, p<0.01), S. sclerotiorum and V. rotundifolia (r = -0.862, p<0.01), A. capillaris (r = -0.902, p<0.01), B. cinerea and T. tetragonoides (r = -0.896, p<0.01) showed an inverse relationship. The rate of mycelial growth inhibition of pathogenic bacteria analysed are as follows: P. ultimum 94%, B. cinerea 50%, C. gloeosporioides 80% in 100% treatment of T. teragonoides. A. capillaris inhibited S. vesicarium by 43%, P. ultimum by 90%; H. hamabo inhibited P. italicum by 50%, S. sclerotiorum by 26%, and F. carica inhibited R. solani by 74%. Total phenol content with antifungal activities are as follows: A. capillaris 16.15 mg/g, F. carica 7.81 mg/g, V. rotundifolia 6.18 mg/g, H. hamabo 5.25 mg/g, T. tetragonoides 4.41 mg/g, and total flavonoid content is as follows: A. capillaris 27.57 mg/g, V. rotundifolia 12.49 mg/g, F. carica 11.45 mg/g, H. hamabo 5.77 mg/g, T. tetragonoides 5.08 mg/g.

Volume Rendering System of e-Science Electron Microscopy using Grid (Gird를 이용한 e-사이언스 전자현미경 볼륨 랜더링 시스템)

  • Jeong, Won-Gu;Jeong, Jong-Man;Lee, Ho;Choe, Sang-Su;Ahn, Young-heon;Hur, Man-Hoi;Kim, Jay;Kim, Eunsung;Jung, Im Y.;Yeom, Heon Y.;Cho, Kum Won;Kweon, Hee-Seok
    • Proceedings of the Korea Contents Association Conference
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    • 2007.11a
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    • pp.560-564
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    • 2007
  • Korea Basic Science Institute(KBSI) has three general electron microscopes including High Voltage Electron Microscope(HVEM) which is the only one in Korea. Observed images through an electron microscope are what they are tilted by each step and saved, offering the more better circumstances for observers, a reconstruction to 3D could be a essential process. In this process, a warping method decreases distortions maximumly of avoided parts of a camera's focus. All these image treatment processes and 3D reconstruction processes are based on an accompaniment of a highly efficient computer, a number of Grid Node Personal computers share this process in a short time and dispose of it. Grid Node Personal computers' purpose is to make an owner can share different each other and various computing resources efficiently and also Grid Node Personal computers is applying to solve problems like a role scheduling needed for a constructing system, a resource management, a security, a capacity measurement, a condition monitoring and so on. Grid Node Personal computers accomplish roles of a highly efficient computer that general individuals felt hard to use, moreover, a image treatment using the warping method becomes a foundation for reconstructing to more closer shape with an real object of observation. Construction of the electron microscope volume 랜더링 system based on Grid Node Personal computer through the warping process can offer more convenient and speedy experiment circumstances to observers, and makes them meet with experiment outcome that is similar to real shapes and is easy to understand.

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Cell Surface Antigenic Relationship of Pathogenic Mycobacteria (병원성 Mycobacteria의 세포표면항원간의 항원적 상관 관계)

  • Kwon, Hyuk-Han;Saito, Hajime;Kim, Sang-Jae
    • Tuberculosis and Respiratory Diseases
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    • v.40 no.5
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    • pp.483-494
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    • 1993
  • Cell surface antigenic relationships between pathogenic mycobacteria have been investigated by the enzyme-linked immunosorbent assay using phenolkilled cells and their rabbits antisera. Homologous and heterologous reactions of Mycobacterium avium-intracellulare antisera before and after homologous and heterologous absorption revealed a close antigenic relationship between strains of the same species and between species if they were members of M. avium(MA)-intracellulare(MI)-scrofulaceum(MG) complex. MAI sera showed a considerable reaction with M. kansasii(MK) and tuberculosis(MTB), but not with the other species. MA(K40004) antiserum reacted with other mycobacteria except few strains of MI and 50~89% of homologous reaction was reduced by heterologous absorption with cells of MI or MS. Intraspecific reaction of MI antisera was natural1y stronger than interspecific reaction and different in extent due to a magnitude of antigenic sharing. Antigenic relationships between N-260D, N-260R, N-260T, and K41014 was somewhat closer than that with N-242D, N-257T, N-28ID, and N-275T. M. nonchromogenicum(MNC) antisera showed a strong interspecific reaction with exception of M. chelonei(MC) and triviale(MTV) to which they reacted weakly or none. Antigenic sharing with M. terrae(MTR) and MG(K30003) was next to intraspecific sharing. NC-3 shared antigens considerably with MA, MC, and M. fortuitum(MF) while NC-11 did not. MTR antisera showed a strong cross-reaction with MI but their homologous reaction was not reduced by MI absorption indicating a paucity of shared antigen of MTR surface. Intraspecific antigenic sharing of course was large with on exception between T-8 and T-13. A considerable amount of antigenic sharing was also found with MNC, MC and MF. Unlike T-8 serum, T-13 antiserum strongly cross-reacted with MA, MG, MK, and MTB. In general, antigenic relationships of mycobacteria, that have been elucidated in this study, well conformed to taxons delineated by the various biological and biochemical means.

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Aided Distance Visual Acuity and Refractive Error Changes by Using Smartphone (스마트폰 사용이 원거리 교정시력과 굴절 이상 변화에 미치는 영향)

  • Kim, Bong-Hwan;Han, Sun-Hee;Shin, Young Gul;Kim, Da Yeong;Park, Jin Young;Sin, Won Chul;Yoon, Jeong Ho
    • Journal of Korean Ophthalmic Optics Society
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    • v.17 no.3
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    • pp.305-309
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    • 2012
  • Purpose:This study was conducted to research any effect on aided distance visual acuity and refractive error changes by using smartphone at near for long term. Methods: 20($20.6{\pm}0.9$ years) young adults subjects with no ocular diseases, over 0.8 of aided distance visual acuity, normal amplitude of accommodation and normal accommodative facility agreed to participate in this study. The subjects were divided into two group, Group 1 (15 cm fixation distance) included 10 subjects and Group 2(40 cm fixation distance) included 10 subjects. Aided distance visual acuity and refractive error were measured before and after using smartphone for 30 minutes by auto-chart project (CP-1000, Dongyang, Korea), phoropter (VT-20, Dongyang, Korea), auto refractor-keratometer (MRK-3100, Huvitz, Korea). After then, the subjects looked at distance with wearing spectacles. Refractive error was measured at 5 minutes, 10 minutes, and 15 minutes later, respectively. Results: After using smartphone at 15 cm for 30 minutes, there was statistically significant reduction of aided distance visual acuity (p=0.030) and increasing myopia (p=0.001). The increased myopia was not statistically significant after 5 minutes rest (p${\geq}$0.464). However there was no statistically significant changes in aided distance visual acuity (p=0.163) and refractive error (p=0.077) after using smartphone at 40 cm for 30 minutes. Conclusions: It is recommend to keep 40 cm off the smartphone from eyes to avoid any aided distance visual acuity and refractive error changes. If smartphone is used closer than 40 cm, a rest for 5 minutes is also recommend after every 30 minutes use with smartphone to avoid any aided distance visual acuity and refractive error changes.

Analysis on Hypothesis-generating Ability of Elementary School Gifted Students in Science and Its Correlation with Meta-cognition (초등과학영재의 가설설정 능력과 메타인지와의 관계 분석)

  • Park, Mijin;Seo, Hae-Ae
    • Journal of Gifted/Talented Education
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    • v.25 no.1
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    • pp.59-76
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    • 2015
  • The study aimed to investigate elementary school gifted students' hypothesis-generating ability and characteristics of hypotheses and to analyze the correlation between hypothesis-generating ability and meta-cognition. Nineteen students enrolled in a science gifted education center affiliated with a university in 2013 were selected as research subjects. An instrument of open ended items about hypothesis generating was developed and administered to students, and their meta-cognition as well as their preferred science teaching method were examined. Hypotheses generated by students were classified into two categories: scientific and non-scientific hypotheses, and then a closer analysis was conducted on characteristics of non-scientific hypotheses. It was found that 47% (18 out of 38 hypotheses) was scientific ones showing that elementary school gifted students in science in this study presented low level of ability in generating hypothesis. It was also found that non-scientific hypotheses frequently showed characteristics of uncertain in causality or impossible to verify relationships. Furthermore, differences in hypothesis-generating ability and characteristics of hypotheses were appeared in conditions whether inquiry questions and variable identification process were given or not. Students showed high abilities in hypothesis generating and variable identifying when inquiry questions and variable identification process were given. Compared to previous research results, students in the study showed high level of meta-cognition and tendency of utilizing monitoring strategy more than planning and regulating. In ill-structured conditions that students themselves find inquiry questions and identify variables, a significant (p<.05) correlation appeared between hypothesis generating ability and meta-cognition and a high level of correlation between planning and regulating strategies. It was also found that differences existed in hypothesis-generating ability and preferred science teaching methods between students with high level and those with low level of meta-cognition; and students with low level of meta cognition showed difficulties in generating hypothesis and identifying variables.

Quantitative Rainfall Estimation for S-band Dual Polarization Radar using Distributed Specific Differential Phase (분포형 비차등위상차를 이용한 S-밴드 이중편파레이더의 정량적 강우 추정)

  • Lee, Keon-Haeng;Lim, Sanghun;Jang, Bong-Joo;Lee, Dong-Ryul
    • Journal of Korea Water Resources Association
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    • v.48 no.1
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    • pp.57-67
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    • 2015
  • One of main benefits of a dual polarization radar is improvement of quantitative rainfall estimation. In this paper, performance of two representative rainfall estimation methods for a dual polarization radar, JPOLE and CSU algorithms, have been compared by using data from a MOLIT S-band dual polarization radar. In addition, this paper presents evaluation of specific differential phase ($K_{dp}$) retrieval algorithm proposed by Lim et al. (2013). Current $K_{dp}$ retrieval methods are based on range filtering technique or regression analysis. However, these methods can result in underestimating peak $K_{dp}$ or negative values in convective regions, and fluctuated $K_{dp}$ in low rain rate regions. To resolve these problems, this study applied the $K_{dp}$ distribution method suggested by Lim et al. (2013) and evaluated by adopting new $K_{dp}$ to JPOLE and CSU algorithms. Data were obtained from the Mt. Biseul radar of MOLIT for two rainfall events in 2012. Results of evaluation showed improvement of the peak $K_{dp}$ and did not show fluctuation and negative $K_{dp}$ values. Also, in heavy rain (daily rainfall > 80 mm), accumulated daily rainfall using new $K_{dp}$ was closer to AWS observation data than that using legacy $K_{dp}$, but in light rain(daily rainfall < 80mm), improvement was insignificant, because $K_{dp}$ is used mostly in case of heavy rain rate of quantitative rainfall estimation algorithm.

Genetic diversity and relationships of Korean, Japanese, and Chinese Jilin provincial wild soybeans (Glycine soja Sieb. and Zucc.) based on SSR markers (한국, 일본 및 중국 지린성 야생콩(Glycine soja Sieb. and Zucc.)의 SSR마커에 의한 유전적 다양성과 유연관계)

  • Jang, Seong-Jin;Park, Su-Jeong;Piao, Xiang-Min;Song, Hang-Lin;Hwang, Tae-Young;Cho, Yong-Gu;Liu, Xian-Hu;Woo, Sun-Hee;Kang, Jung-Hoon;Kim, Hong-Sig
    • Korean Journal of Breeding Science
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    • v.42 no.1
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    • pp.87-99
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    • 2010
  • Genetic diversity and relationships within and among Korean, Japanese and Chinese Jilin provincial wild soybeans based on SSR markers were evaluated to enlarge genetic variation in soybean breeding in the future. A total of 184 wild soybeans including 67 Korean, 71 Japanese and 46 Chinese Jilin provincial wild soybeans were analyzed to evaluate genetic diversity and relationships based on 23 SSR markers. Korean and Japanese wild soybeans were obtained from National Agrobiodiversity Center, Korea, and Biological Resource Center in Lotus and Glycine, Frontier Science Research Center, University of Miyazaki, Japan, respectively. Chinese wild soybeans were collected from Jilin province, China. Twenty three SSR markers generated a total of 964 alleles with an average of 41.9 alleles per marker. Number of alleles ranged from 23 (Satt635) to 56 (Satt157). Genetic diversity (PIC value) of 184 wild soybeans ranged from 0.880 to 0.968 with an average of 0.945. Number of alleles for Korean, Japanese and Chinese Jilin provincial wild soybeans was 513 with an average of 22.3, 511 with an average of 22.2, and 312 with an average of 13.6 per marker, respectively. PIC value for Korean, Japanese and Chinese Jilin provincial wild soybeans was similar with an average of 0.905, 0.897, and 0.850, respectively. Cluster analysis based on genetic distances estimated by SSR markers classified wild soybeans into 3 clusters. Cluster I included only Chinese Jilin provincial wild soybeans. Cluster II included most of Japanese wild soybeans including 5 Korean wild soybeans. Cluster III included most of Korean wild soybeans including 6 Japanese and 1 Chinese Jilin provincial wild soybeans. Cluster I was not subclassified, but cluster II and III were subclassified into various groups. Genetic distance evaluated by SSR markers between Korean and Japanese wild soybeans was closer than that of between Korean and Chinese Jilin provincial, and between Japanese and Chinese Jilin provincial wild soybeans.