Browse > Article
http://dx.doi.org/10.4047/jkap.2012.50.4.285

A study of mesenchymal stem cell proliferation and surface characteristics of the titanium discs coated with MS275/PLGA by an electrospray  

Yoo, Soo-Yeon (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University)
Kim, Seong-Kyun (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University)
Heo, Seong-Joo (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University)
Koak, Jai-Young (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University)
Lee, Joo-Hee (Department of Prosthodontics, Seoul Asan Medical Center, Ulsan University)
Park, Yoon-Kyung (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University)
Kim, Ena (Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University)
Publication Information
The Journal of Korean Academy of Prosthodontics / v.50, no.4, 2012 , pp. 285-291 More about this Journal
Abstract
Purpose: This study was conducted to identify the surface characteristics of titanium discs coated with MS275/PLGA by electrospray and which is effective to mesenchymal stem cell proliferation. Materials and methods: We used anodized surface coated with PLGA as a control group and anodized surface coated with MS275 $0.5{\mu}M$, $1{\mu}M$, $1.5{\mu}M$ as test groups. To examine that the coating particles are nanometer sized, FE-SEM was used and AFM was utilized to determine the difference of coating surface roughness. We checked the mesenchymal stem cell proliferation by using MTT assay on $1^{st}$, $4^{th}$, $7^{th}$ days. Results: There was no significant difference between control groups and test groups in AFM results (P>.05). In MTT assay results, mesenchymal stem cell proliferation was increased with time, at $7^{th}$ day, cell viability on discs coated with $1.5{\mu}M$ MS275 was significantly higher than control group (P<.05). As SEM showed, the number of cells on all discs was increased and the morphology of cell attachment was also wider and closer with time. Conclusion: Titanium surface coated with MS275/PLGA showed significantly higher cell proliferation and the more density of MS275 was dispersed on titanium discs, the faster cells grew.
Keywords
Titanium; MS275; PLGA; Mesenchymal stem cells;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Ivanoff CJ, Widmark G, Hallgren C, Sennerby L, Wennerberg A. Histologic evaluation of the bone integration of TiO2 blasted and turned titanium microimplants in humans. Clin Oral Implants Res 2001;12:128-34.   DOI   ScienceOn
2 Orsini G, Assenza B, Scarano A, Piattelli M, Piattelli A. Surface analysis of machined versus sandblasted and acid-etched titanium implants. Int J Oral Maxillofac Implants 2000;15:779-84.
3 Zhu X, Chen J, Scheideler L, Altebaeumer T, Geis-Gerstorfer J, Kern D. Cellular reactions of osteoblasts to micron- and submicronscale porous structures of titanium surfaces. Cells Tissues Organs 2004;178:13-22.   DOI   ScienceOn
4 Huang HH, Ho CT, Lee TH, Lee TL, Liao KK, Chen FL. Effect of surface roughness of ground titanium on initial cell adhesion. Biomol Eng 2004;21:93-7.   DOI   ScienceOn
5 Kim HN, Lee JH, Bae SC, Ryoo HM, Kim HH, Ha H, Lee ZH. Histone deacetylase inhibitor MS-275 stimulates bone formation in part by enhancing Dhx36-mediated TNAP transcription. J Bone Miner Res 2011;26:2161-73.   DOI   ScienceOn
6 Cho YJ, Heo SJ, Koak JY, Kim SK, Lee JH. Cellular responses on anodized titanium discs coated with $1{\alpha}$,25-dihydroxyvitamin D3 incorporated Poly (D,L-lactide-co-glycolide) (PLGA) nanoparticles. J Korean Acad Prosthodont 2008;46:620-627.   DOI   ScienceOn
7 Lee SY. Effect of poly(D,L-lactide-co-glycolide)/bone morphogenic protein-2 coating of anodized titanium surface on osteoblast-like cells. MS Thesis. In: Korea, Seoul University, 2010.
8 Freiberg S, Zhu XX. Polymer microspheres for controlled drug release. Int J Pharm 2004;282:1-18.   DOI   ScienceOn
9 Sims NA, Gooi JH. Bone remodeling: Multiple cellular interactions required for coupling of bone formation and resorption. Semin Cell Dev Biol 2008;19:444-51.   DOI   ScienceOn
10 Agrawal CM, Niederauer GG, Athanasiou KA. Fabrication and Characterization of PLA-PGA Orthopedic Implants. Tissue Eng 1995;1:241-52.   DOI
11 Hahn H, Palich W. Preliminary evaluation of porous metal surfaced titanium for orthopedic implants. J Biomed Mater Res 1970;4:571-7.   DOI
12 Sammons RL, Lumbikanonda N, Gross M, Cantzler P. Comparison of osteoblast spreading on microstructured dental implant surfaces and cell behaviour in an explant model of osseointegration. A scanning electron microscopic study. Clin Oral Implants Res 2005;16:657-66.   DOI   ScienceOn
13 Geesink RG, de Groot K, Klein CP. Bonding of bone to apatitecoated implants. J Bone Joint Surg Br 1988;70:17-22.
14 de Groot K, Geesink R, Klein CPAT, Serekian P. Plasma sprayed coatings of hydroxylapatite. J Biomed Mater Res 1987;21:1375-81.   DOI   ScienceOn
15 Catledge SA, Vohra YK, Bellis SL, Sawyer AA. Mesenchymal stem cell adhesion and spreading on nanostructured biomaterials. J Nanosci Nanotechnol 2004;4:986-9.   DOI   ScienceOn
16 Hans ML, Lowman AM. Biodegradable nanoparticles for drug delivery and targeting. Curr Opin Solid State Mater Sci 2002;6:319-27.   DOI   ScienceOn
17 Wong M, Eulenberger J, Schenk R, Hunziker E. Effect of surface topography on the osseointegration of implant materials in trabecular bone. J Biomed Master Res 1995;29:1567-76.   DOI
18 Xie J, Baumann MJ, McCabe LR. Osteoblasts respond to hydroxyapatite surfaces with immediate changes in gene expression. J Biomed Mater Res A 2004;71:108-17.
19 Hermann JS, Cochran DL, Nummikoski PV, Buser D. Crestal bone changes around titanium implants. A radiographic evaluation of unloaded nonsubmerged and submerged implants in the canine mandible. J Periodontol 1997;68:1117-30.   DOI   ScienceOn
20 Sul YT, Johansson CB, Petronis S, Krozer A, Jeong Y, Wennerberg A, Albrektsson T. Characteristics of the surface oxides on turned and electrochemically oxidized pure titanium implants up to dielectric breakdown: the oxide thickness, micropore configurations, surface roughness, crystal structure and chemical composition. Biomaterials 2002;23:491-501.   DOI   ScienceOn