• Journal of Korean Neurosurgical Society
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• 제64권3호
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• pp.418-426
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• 2021

Objective : A role of diffusion-weighted imaging (DWI) in the diagnosis of cerebral venous thrombosis (CVT) is not well-understood. This study evaluates the effectiveness of DWI in the diagnosis of CVT. Methods : Literature search was conducted in electronic databases for the identification of studies which reported the outcomes of patients subjected to DWI for CVT diagnosis. Random-effects meta-analyses were performed to achieve overall estimates of important diagnostic efficiency indices including hyperintense signal rate, the sensitivity and specificity of DWI in diagnosing CVT, and the apparent diffusion coefficient (ADC) of DWI signal areas and surrounding tissue. Results : Nineteen studies (443 patients with 856 CVTs; age 40 years [95% confidence interval (CI), 33 to 43]; 28% males [95% CI, 18 to 38]; symptom onset to DWI time 4.6 days [95% CI, 2.3 to 6.9]) were included. Hyperintense signals on DWI were detected in 40% (95% CI, 26 to 55) of the cases. The sensitivity of DWI for detecting CVT was 22% (95% CI, 11 to 34) but specificity was 98% (95% CI, 95 to 100). ADC values were quite heterogenous in DWI signal areas. However, generally the ADC values were lower in DWI signal areas than in surrounding normal areas (mean difference-0.33×10-3 ㎟/s [95% CI, -0.44 to -0.23]; p<0.00001). Conclusion : DWI has a low sensitivity in detecting CVT and thus has a high risk of missing many CVT cases. However, because of its high specificity, it may have supporting and exploratory roles in CVT diagnosis.

• 동양고전연구
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• 제34호
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• pp.167-206
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• 2009

본고는 금영작(金永爵)이 중국 문인들로부터 받은 편지를 모아놓은 척독집 "중조학사서한록(中朝學士書翰錄)"을 새로 발굴하고 소개한 글로, 정공수(程恭壽), 옹학함(翁學涵), 장병염(張丙炎), 소언한(少言翰)과 자렴함(子廉銜), 이문원(李文源) 등이 김영작에게 보낸 편지 10통, 조정황(趙廷璜)이 김영작의 아들 김홍집(金弘集)에게 보낸 편지 1통 및 시(詩) 5수가 수록되어 있다. 김영작은 많은 중국 문인들과 직접 또는 간접적인 교유를 진행하였다. 그는 연행을 갔다 온 친구 홍양후(洪良厚)의 소개로 중국 문인 이백형(李伯衡), 수방울(帥方蔚)과 편지를 주고받으며 30여 년간 간접적인 교유를 진행하였다. 1858년에는 동지사(冬至使)의 부사(副使)로 직접 북경에 가서 현지 중국 문사 섭명례(葉名澧), 장병염(張丙炎), 오곤전(吳昆田), 정공수(程恭壽), 조광(趙光) 등과 만나서 교유하고 귀국 후에도 지속적으로 척독교류를 진행하였다. 김영작은 학계에 별로 알려지지 않은 인물이나 한중 문화교류에서 빼놓을 수 없는 중요한 인물이다. 김영작이 생면부지의 중국 문사 이백형(李伯衡)과 수방울(帥方蔚)에게 먼저 편지를 보내어 교유를 청하였고 상대방 역시 흔쾌히 답장을 보내면서 평생 편지로만 문학적, 개인적 교류를 진행하였으며, 또한 김영작이 조선의 명문가의 사대부로서 연행사(燕行使)의 부사(副使)라는 높은 신분으로 중국 현지의 문사들과 친밀하게 교유할 수 있었던 것은 그만큼 한중 인사들의 마음이 열려있고 신뢰성과 친숙감이 전제되어 있다는 사실을 알 수 있다. 이는 홍대용을 비롯한 많은 선배 문인들의 교유의 전통이 있어 기초를 닦아놓았기에 가능했다. 이런 의미에서 19세기 후반 김영작과 중국 문사들의 교유는 중요한 의미를 지니며 그 사실을 보여주는 척독집 "중조학사서한록(中朝學士書翰錄)"도 중요한 자료적 가치를 갖고 있다. 다만 아쉬운 점은 "중조학사서한록(中朝學士書翰錄)"만으로는 김영작과 중국 문사들이 주고받은(교류의) 내용을 완전하게 파악할 수 없다는 자료적 한계를 갖고 있는 점이다. 앞으로 중국 측에 교유 기록이 남아있는 자료들을 더욱 많이 발굴해낼 것을 기대할 뿐이다.

• Asian-Australasian Journal of Animal Sciences
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• 제33권4호
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• pp.531-538
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• 2020

Objective: Evidence from previous reports indicates that pig domestication in East Asia mainly occurred in the Mekong region and the middle and downstream regions of the Yangtze River. Further research identified two new origin centers for domestic pigs in the Tibetan Plateau and the islands of Southeast Asia. However, due to the small sample size of Tibetan pigs, details of the origin and spread of Tibetan pigs has not yet been established. Methods: We analyzed mitochondrial DNA control region (D-loop) variation in 1,201 individuals from nine Tibetan pig populations across five provinces. Comprehensive Tibetan pig samples were taken to perform the most detailed analysis of Tibetan pigs to date. Results: The result indicate that Rkaze pigs had the lowest level of diversity, while Changdu pigs had the highest diversity. Interestingly, these two populations were both in the Tibetan Plateau area. If we calculate diversity in terms of each province, the Tibetan Plateau area had the lowest diversity, while the Chinese province of Gansu had the highest diversity. Diversity gradient analysis of major haplotypes suggested three domestication centers of Tibetan pigs in the Tibetan Plateau and the Chinese provinces of Gansu and Yunnan. Conclusion: We found two new domestication centers for Tibetan pigs. One is in the Chinese province of Gansu, which lies in the upstream region of the Yellow River, and the other is in the Chinese province of Yunnan.

• BMB Reports
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• 제45권8호
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• pp.470-475
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• 2012

Protein arginine methyltransferase 1 (PRMT1), a type-I arginine methyltransferase, has been implicated in diverse cellular events. We have focused on the role of PRMT1 in gliomagenesis. In this study, we showed that PRMT1 expression was up-regulated in glioma tissues and cell lines compared with normal brain tissues. The knock-down of PRMT1 resulted in an arrest in the G1-S phase of the cell cycle, proliferation inhibition and apoptosis induction in four glioma cell lines (T98G, U87MG, U251, and A172). Moreover, an in vivo study confirmed that the tumor growth in nude mouse xenografts was significantly decreased in the RNAi-PRMT1 group. Additionally, we found that the level of the asymmetric dimethylated modification of H4R3, a substrate of PRMT1, was higher in glioma cells than in normal brain tissues and decreased after PRMT1 knock-down. Our data suggest a potential role for PRMT1 as a novel biomarker of and therapeutic target in gliomas.

• Journal of Microbiology and Biotechnology
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• 제25권12호
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• pp.2135-2145
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• 2015

VP7, an outer capsid protein of grass carp reovirus (GCRV), was expressed and displayed on the surface of Saccharomyces cerevisiae for developing an efficient vaccine against hemorrhagic disease of grass carp. The result of flow cytometry analysis indicated that protein VP7 could be displayed on the surface of yeast cells after inducing with galactose. The expression of VP7 was confirmed by western blot analysis and further visualized with confocal microscopy. The specific antibodies against VP7 generated from mice were detectable from all immune groups except the control group, which was immunized with untransformed yeast cells. The displaying VP7 on glycosylation-deficient strain EBYΔMnn9 was detected to induce a relatively low level of specific antibody amongst the three strains. However, the antiserum of EBYΔM9-VP7 showed relative high capacity to neutralize GCRV. Further neutralization testing assays indicated that the neutralizing ability of antiserum of the EBYΔM9-VP7 group appeared concentration dependent, and could be up to 66.7% when the antiserum was diluted to 1:50. This result indicates that appropriate gene modification of glycosylation in a yeast strain has essential effect on the immunogenicity of a yeast-based vaccine.

• Journal of Ginseng Research
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• 제48권1호
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• pp.103-111
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• 2024

Background: Ginseng (Panax ginseng Mayer) is an important natural medicine. However, a long culture period and challenging quality control requirements limit its further use. Although artificial cultivation can yield a sustainable medicinal supply, research on the association between the transplantation and chaining of metabolic networks, especially the regulation of ginsenoside biosynthetic pathways, is limited. Methods: Herein, we performed Liquid chromatography tandem mass spectrometry based metabolomic measurements to evaluate ginsenoside accumulation and categorise differentially abundant metabolites (DAMs). Transcriptome measurements using an Illumina Platform were then conducted to probe the landscape of genetic alterations in ginseng at various ages in transplantation mode. Using pathway data and crosstalk DAMs obtained by MapMan, we constructed a metabolic profile of transplantation Ginseng. Results: Accumulation of active ingredients was not obvious during the first 4 years (in the field), but following transplantation, the ginsenoside content increased significantly from 6-8 years (in the wild). Glycerolipid metabolism and Glycerophospholipid metabolism were the most significant metabolic pathways, as Lipids and lipid-like molecule affected the yield of ginsenosides. Starch and sucrose were the most active metabolic pathways during transplantation Ginseng growth. Conclusion: This study expands our understanding of metabolic network features and the accumulation of specific compounds during different growth stages of this perennial herbaceous plant when growing in transplantation mode. The findings provide a basis for selecting the optimal transplanting time.

• 해양환경안전학회지
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• 제10권1호
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• pp.41-50
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• 2004

중국의 조선기술은 유구하고 찬란한 발전역사를 가지고 있으며 세계 고대 조선기술사에서 아주 중요한 자리를 차지하고 있다. 지금까지 조선기술사의 연구경향은 서양 조선기술사에 편중되어 있었기 때문에 중국 조선기술사에 관한 연구는 이러한 결함을 보완 할 것이다, 인류사회의 발전과 더불어 조선기술도 급속히 발전되었다. 송대(960-1279년)에 이르러 중국의 조선업은 크게 발전되었으며, 그 특징으로는 선박의 대형화, 조선기지와 선박수량의 현저한 증가, 조선기술의 제고와 설비의 발전으로 나타난다. 송대의 조선기술은 원명(元明)시대의 조선기술 발전에 토대를 마련하였다. 따라서 교량역할을 한 송대의 조선기술을 연구함으로써 중국의 조선기술의 발전사를 알아볼 수 있을 것이다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 춘계학술발표회 요지
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• pp.228-229
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• 2005

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2018년도 춘계 종합학술대회 논문집
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• pp.193-194
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• 2018

본 연구는 샤오취안의 "우리들 세대"를 중심으로 중국의 인물 사진과 시대 배경적의 관계를 연구한다. 샤오취안은 인물의 생활 배경 및 복장, 인물의 표정에 나타나는 감정, 구도를 활용해 시대적 배경을 상징적으로 구현했다. 본 연구는 중국 인물 사진을 대표하는 샤오취안의 "우리들 세대" 작품을 통해 중국 인물 사진의 역사적 기록으로서의 가치를 제고하는 기회를 제공한다.

• BMB Reports
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• 제43권7호
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• pp.485-490
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• 2010

Neurotrophins regulate many aspects of neuronal function through activation of the high affinity Trk receptors. Shc family proteins are implicated in the coupling of RTK to the Ras/mitogen-activated protein kinase signaling cascade. Here we report that the fourth Shc family member, ShcD, associates with TrkB receptor and regulates BDNF-induced MAPK activation. Yeast two-hybrid assay and Co-IP experiments demonstrate ShcD interacts with TrkB in a kinase-activity-dependent manner. Confocal analysis shows ShcD cololizes well with TrkB in transfected 293T cells. Subsequent mapping experiments and mutational analysis indicate that both PTB and SH2 domains are capable of binding to TrkB and PTB domain binds to TrkB NPQY motif. Furthermore, ShcD is involved in BDNF-induced MAPK activation. In summary, we demonstrate that ShcD is a substrate of TrkB and mediates TrkB downstream signaling pathway.