• 제목/요약/키워드: Thawing period

검색결과 99건 처리시간 0.024초

Damage Mechanism of Drift Ice Impact

  • Gong, Li;Wang, Zhonghui;Li, Yaxian;Jin, Chunling;Wang, Jing
    • Journal of Information Processing Systems
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    • 제15권6호
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    • pp.1350-1364
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    • 2019
  • The ice damage occurs frequently in cold and dry region of western China in winter ice period and spring thaw period. In the drift ice condition, it is easy to form different extrusion force or impact force to damage tunnel lining, causing project failure. The failure project could not arrive the original planning and construction goal, giving rise to the water allocation pressure which influences diversion irrigation and farming production in spring. This study conducts the theoretical study on contact-impact algorithm of drift ices crashing diversion tunnel based on the symmetric penalty function in finite element theory. ANSYS/LS-DYNA is adopted as the platform to establish tunnel model and drift ice model. LS-DYNA SOLVER is used as the solver and LS-PREPOST is used to do post-processing, analyzing the damage degrees of drift ices on tunnel. Constructing physical model in the experiment to verify and reveal the impact damage mechanism of drift ices on diversion tunnel. The software simulation results and the experiment results show that tunnel lining surface will form varying degree deformation and failure when drift ices crash tunnel lining on different velocity, different plan size and different thickness of drift ice. The researches also show that there are damages of drift ice impact force on tunnel lining in the thawing period in cold and dry region. By long time water scouring, the tunnel lining surfaces are broken and falling off which breaks the strength and stability of the structure.

냉동침을 이용한 폐 냉동수술의 동물실험: 냉동수술 방법의 비교 실험 (Cryosurgery of Lung with 2.4 mm Cryoprobe: An Experimental in vivo Study of the Cryosurgery in Canine Model)

  • 김광택;정봉규;이성호;조종호;손호성;방영호;선경;박성민
    • Journal of Chest Surgery
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    • 제39권7호
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    • pp.520-526
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    • 2006
  • 배경: 폐암치료에 있어 냉동수술은 저온에서 폐조직의 반응에 대한 연구 자료가 많지 않아 임상적용은 매우 제한적이다. 본 실험의 목적은 초저온에서 폐조직의 반응과 온도변화에 따른 폐의 조직학적 변화에 대해 조사하는 것이다. 대상 및 방법: 12마리의 잡견을 전신마취 후 5번째 늑간을 열어 폐를 노출시켰다. 2.4 mm 냉동침을 폐표면으로부터 20 mm 깊이로 폐조직으로 삽입하고 냉동침으로부터 5 mm 간격으로 온도센서(T1-4)를 삽입하였다. 실험견을 A군(n=8)과 B군(n=4)으로 나누어 A군은 20분간 $-120^{\circ}C$로 냉동한 후 5분간 해동하였다가 다시 20분간 냉동하였으며, B군에서는 40분간 해동과정 없이 $-120^{\circ}C$로 냉동하였다. 냉동수술 후 1일째 폐조직을 적출하였으며 A군 중 4마리는 지연된 조직반응을 보기 위해 7주일째 폐조직을 적출하여 조직학적 검사를 하였다. 결과: A군에서 일차 냉동 시 T1과 T2의 온도가 각각 $4.1{\pm}11^{\circ}C$$31{\pm}5^{\circ}C$로 떨어졌으며 이차 냉동 시 온도는 더욱 하강하였다: T1 $-56.4{\pm}9.7^{\circ}C,\;T2\;18.4{\pm}14.2^{\circ}C,\;T3\;18.5{\pm}9.4^{\circ}C\;and\;T4\;35.9{\pm}2.9^{\circ}C$. 일차 냉동시기와 이차냉동 시의 온도-거리 그래프를 비교한 결과 온도-거리 관계가 곡선에서 선형으로 변화했다. B군에서는 온도센서의 온도가 감소하였으며 냉동 40분 동안 변화 없었다. A군에서 광학현미경상 지름 $18.6{\pm}6.4mm$의 출혈성 괴사의 소견을 볼 수 있었다. B군에서의 괴사부위 지름은 $14{\pm}3mm$였다. 괴사된 부위에서 살아 있는 세포를 발견할 수 없었다. 결론: 냉동 후 해동과정은 폐조직의 전도율을 변화시키며 이는 이차 냉동 시 폐조직의 온도를 더욱 떨어뜨리고 세포파괴의 범위를 넓힌다.

Safety Inspection on Jeotgal, Salt-Fermented Sea Food

  • Park Mi-Yeon;Lee Myung-Suk;Chang Dong-Suck
    • Fisheries and Aquatic Sciences
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    • 제5권1호
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    • pp.43-47
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    • 2002
  • Jeotgal, salt- fermented sea food, is a kind of Korean traditional foods. We carried out hazard analysis on Changran (stomach and intestine of Alaska pollack) Jeotgal and squid Jeotgal through the whole processing procedures at H Co. located at Guryongpo from April 2000 to September 2001. During this studying period, we educated employee regularly and analyzed hazards on the whole process and then could conclude that pathogenic bacteria and metal particles were most important hazards in Jeotgal. Metal particles in end-product will be eliminated by laser detector before packaging. But bacteria must thoroughly manage through the whole process. Bacteriological qualities of the end products were much improved after education for the employees and by effort for HACCP (Hazard Analysis and Critical Control Point) program introduction. Pathogenic bacteria such as pathogenic Escherichia coli, Salmonella spp., coagulase positive Staphylococcus aureus and Vibrio parahaemolyticus were not detected from not only raw materials but also end products. The falling bacteria in the places such as thawing area, packaging area, seasoning area, fermenting room, subsidiary materials room and storage room were less than 30 CFU per plate for 30 minutes during working time. But those were increased more than 10 times during the resting time. It means that special measures are needed during the break time such as lunch time or exchanging working teams.

바이오 폴리머 콘크리트의 환경 저항성 평가 연구 (A Evaluation of Environmental Resistance for Bio-Polymer Concretes)

  • 김제원;김태우;박희문;김부일
    • 한국도로학회논문집
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    • 제15권5호
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    • pp.75-79
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    • 2013
  • PURPOSES : The objective of this study is to evaluate the environmental resistance of bio-polymer concrete for use of pavement materials developed for reducing the carbon-dioxide. METHODS : The compression, tension, and bending strength tests were conducted on the bio-polymer concrete specimens with and without environmental conditioning. The specimens were conditioned using the freezing-thaw and accelerated weathering process for long period of time. To assess the resistance against chloride, the chloride ion penetration resistance tests were carried out on the bio-polymer concrete specimens. RESULTS : Test results show that the maximum difference in strength between specimens with and without conditioning is about 2.6MPa indicating that the effect of environmental conditioning on specimen strength is negligible. Based on the chloride ion penetration resistance test, the penetration quantity of electric charge of the specimens is zero and there is no ion penetration within the bio-polymer concrete. CONCLUSIONS : It is found from this study that there is slight change in strength of bio-polymer concretes before and after environmental conditioning process and no chloride ion penetration observed in these specimens. Therefore, the developed bio-polymer concretes can be applied effectively as pavement materials due to the small change of physical properties with environment change.

3차원 공간 좌표 시스템과 드론 영상 검출을 활용한 산사태 안식각 예측에 관한 연구 (Landslide Prediction with Angle of Repose Prediction Using 3D Spatial Coordinate System and Drone Image Detection)

  • 추용주;임수영;이승엽
    • 스마트미디어저널
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    • 제12권3호
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    • pp.77-84
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    • 2023
  • 산림 화재는 현대의 급격한 기후 변화에 따른 대표적인 자연 재해이다. 화재로 인한 산림 훼손으로 산림 조성이 미비한 시점에 겨울철 해빙기와 폭우 등으로 산사태가 발생하는 이차적인 피해가 발생한다. 대부분 국가에서는 산림 관리를 위해 CCTV 중심의 모니터링 시스템으로 제한적인 면적만 관리하고 있다. 산사태 징후를 예측하기 위해 사전에 3차원 공간 좌표를 포함하고 있는 마커를 위험지역의 경사면에 장착한 후, 주기적인 드론 촬영으로 3차원 맵핑 및 안식각을 검출한다. 이를 위해서 마커의 인식범위 및 화각을 정의하고, 산사태 징후를 사전에 예측하는 방법을 제시하였다.

고랭지 밭 유역의 융설에 의한 수질특성 (Characteristics of Water Quality in Basin of Alpine Field during the Snowmelt Season)

  • 최용훈;원철희;박운지;신민환;신재영;이수인;최중대
    • 한국물환경학회지
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    • 제28권3호
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    • pp.453-459
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    • 2012
  • In this study, Song-Cheon river Basin located at Dae-kwan-lyong Meyn in Kang-Won Province was monitored to analyze characteristics of non-point source pollution during the snowmelt period. The characteristics of NPS pollution such as runoff, change in water quality and pollutant loads by snowmelt were investigated from 23th February 2010 to 27th April 2010. The results showed that The climate change effects flow, turbidity and concentration of SS and COD with showing similar trend of fluctuation. Daily pollutant loads per unit area were SS 74.43 kg/ha/day, COD 1.25 kg/ha/day, BOD 0.21 kg/ha/day, TN 0.717 kg/ha/day, TP 0.141 kg/ha/day and the flow weight mean concentration has been SS 985.97 mg/L, COD 16.28 mg/L, BOD 2.67 mg/L, TN 9.302 mg/L, TP 1.834 mg/L, respectively. The flow and pollutant loads both of SS and of COD significantly increased during the snowmelt period. Because structurally loosen soil by freezing and thawing deteriorates water quality, research and management measures will be needed.

1단계 straw동결법이 소 수정란의 생존성에 미치는 영향에 관한 연구 (A Study on the Effects of Cryopreservation by One-Step Straw Method on the Survival of Bovine Embryos)

  • 김상근;김무강
    • 한국수정란이식학회지
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    • 제9권1호
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    • pp.65-71
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    • 1994
  • This study were carried out to investigate the effective concentration of cryoprotectant agents and sucrose by one-step straw method, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos. The bovine embryos following dehydration by cryoprotective agents and a various concentration of sucrose were directly plunged into liquid nitrogen and thawed in 3O$^{\circ}C$ water. Survival rate was defined by FDA test. The results are summarized as follows : 1. The survival rate of bovine embryos thawed after rapid freezing in the freezing medium containing a various kinds of cryoprotective agents added 0.25M and O.50M sucrose were 28.6% and 25.0%, 35.1% and 31.6%, 32.4% and 24.4%, 34.2% and 28.2%, 18.9% and 17.6%, 14.7.% and 21.6%, respectively. 2. The survival rate of bovine embryos thawed after rapid freezing in the freezing medium containing a various concentration of sucrose added 1.5M and 2.OM glycerol, i.5M and 2.OM DMSO and 1.5M and 2.OM propanediol were 22.9~37.8%, 2O.7~31. 3%, 19.2~30.0% and 17.2~25.0%, respectively. 3. The temperature thawed at 2$0^{\circ}C$ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 3$0^{\circ}C$ and 35$^{\circ}C$. 4. The equilibration time on the survival rate of bovine embryos was attained after short period of time(2.5~5 min.) in the freezing medium higher than long period of time (1O~20 min.).

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한라산 성판악 등산로 노폭의 확대 속도와 요인 (Rates and Factors of Path Widening in Seongpanak Hiking Trail of Mount Halla, Jeju Island)

  • 김태호
    • 대한지리학회지
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    • 제43권3호
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    • pp.296-311
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    • 2008
  • 한라산 성판악 코스 표고 $875{\sim}$1,400m 구간의 32개 지점에서 등산로 측벽의 후퇴량, 즉 노폭의 확대 속도를 관측하고, 확대 프로세스에 영향을 미치는 요인을 조사하였다. 2002년 10월부터 2008년 4월까지 발생한 측벽의 평균 후퇴량은 50.6mm로서 후퇴 속도는 10.0mm/yr이다. 연도별로는 2006년과 2003년에 각각 최대치 18.0mm/yr와 최소치 7.6mm/yr를 기록하였다. 관측 기간을 동결기와 비동결기로 구분하면 시기별 후퇴 속도는 19.3mm/yr와 4.3mm/yr이다. 비동결기를 다시 우기와 건기로 구분하면 후퇴 속도는 각각 5.9mm/yr와 2.9mm/yr이다. 계절별로는 겨울철(42.2mm/yr), 봄철(13.0mm/yr), 가을철(6.4mm/yr), 장마철(3.3mm/yr), 여름철(1.3mm/yr)의 순이다. 토양경도와 표고는 측벽의 후퇴 속도와 명료한 상관관계를 보이지 않으나, 동결기에는 남향보다 북향 측벽에서 후퇴 쏙도가 더 크다. 겨울철 후퇴량이 전체 후퇴량의 76.7%를 차지하고 있어 등산로 측벽은 주로 서릿발 작용으로 후퇴하는 것으로 판단된다. 조사 구간은 연간 85일 정도 서릿발이 발생할 수 있는 기후 조건을 지니고 있으며, 12월의 동결 진행기, 3월과 4월의 융해 진행기에는 서릿발로 덮여 있는 측벽의 모습을 쉽게 볼 수 있다. 반면에 한라산 동사면의 약한 풍속과 교목 지대에 위치하고 있는 등산로 특성상 취식과 우적침식은 탁월하지 않다. 등산로 노면의 포장 시설로 인하여 우세의 영향도 미약하며, 동물의 영향도 나타나지 않는다. 그러나 봄철에 융설로 생긴 물웅덩이를 피하여 걷는 등산객 때문에 크게 후퇴하는 측벽도 보인다.

Effects of Sucrose and Trehalose on the Freezability of Markhoz Goat Spermatozoa

  • Khalili, B.;Farshad, Abbas;Zamiri, M.J.;Rashidi, A.;Fazeli, P.
    • Asian-Australasian Journal of Animal Sciences
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    • 제22권12호
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    • pp.1614-1619
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    • 2009
  • The present study was conducted to observe the effect of increased osmolality of basic tris extender supplemented with trehalose and sucrose on post-thawing quality (motility, progressive motility, viability, the rate of acrosome abnormality, total abnormality and membrane integrity) of Markhoz goat spermatozoa. Fresh semen samples were evaluated for motility and sperm concentration. Only semen samples with motility more than 70% and sperm concentration higher than $3{\times}10^{9}$ sperm/ml were used for cryopreservation. In Exp. 1, trehalose (50, 75 or 100 mM) and sucrose (40, 60 or 80 mM) were added to a basic tris diluent. Based on the results of experiment 1, the goal of Exp. 2 was to investigate the combinational effects of the highest and lowest concentrations ($T_{100}+S_{80}$ or $T_{50}+S_{40}$) of trehalose and sucrose. As the control, semen was diluted and frozen in the tris diluent without trehalose or sucrose. The results in Exp. 1 showed that all evaluated spermatozoa characteristics improved significantly after freezing and thawing (p<0.05) and at the same time the increase of trehalose and sucrose concentrations in basic extenders was seen, with the best results obtained for extenders containing 70 and 100 mM trehalose and 80 mM sucrose. Comparing these results with those of control diluents, the effects of supplementation were significantly (p<0.05) better. In Exp. 2, the results showed no significant differences (p>0.05) between $T_{100}+S_{80}$ and $T_{50}+S_{40}$ extenders, but the results of $T_{50}+S_{40}$were slightly better than obtained with $T_{100}+S_{80}$ diluents. Furthermore, the results of this experiment indicated that the sperm characteristics in the isotonic control extender were significantly (p<0.05) lower than examined extenders. In conclusion, the results of this study indicated that goat sperm can tolerate hypertonic trehalose and sucrose solutions better than isotonic control diluents in the freezing period. In particular, these positive effects have been shown for acrosome integrity, which is very important for the fertilization capacity of sperm. The data indicated that addition of trehalose plus sucrose to the freezing extender can be recommended for cryopreservation of goat spermatozoa, but more data is needed on pregnancy rate, acrosome reaction and IVF to ascertain the real effect.

가금의 인공수정 (Artificial Insemination in Poultry)

  • Howarth, Birkett
    • 한국가축번식학회지
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    • 제7권2호
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    • pp.57-71
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    • 1983
  • 1. Diluted chicken semen can be preserved at 2 to 5$^{\circ}C$ for 24 to 48 hr with resultant fertility of greater than 90% of that of fresh semen. Turkey semen can be preserved at 10 to 15$^{\circ}C$ for 6 to 24 hr and provide economical fertility. 2. Frozen chicken semen has given variable results; a 21 to 93% fertility ranges as compared to 92 to 94% expected with fresh semen. Highest fertility levels obtained with frozen turkey semen intravaginally inseminated have been 61 and 63% using DMSO and glycerol, respectively, as cryoprotectants. 3. The use of glycerol as a cryoprotectant reauires that its concentration in semen be reduced to less than 2% either by dialysis or centrifugation after thawing and before intravaginal insemination if optimal fertility is to be obtained. 4. The temperature at which cryoprotectants are added to semen and the time allowed for equilibration are important for subsequent fertility pre- and post-freezing. 5. The type of container used for packaging the semen, freeze or cooling rates, thaw rates and level of cryoprotectant all interact in affecting cell survival. 6. Plastic freeze straws as a packaging device for semen offers the following advantages: easy to handle, require minimal storage space, offer a wide range of freeze and thaw rates, and insemination can be made directly from them upon thawing. 7. Controlled slow cooling rates of 1 to 8$^{\circ}C$/min have thus far provided the best results for cooling chicken semen throught the transition phase change (liquid to solid) or critical temperature range of +5 to -20 or -35$^{\circ}C$. 8. Highest fertilities have been achieved with frozen chicken semen where a slow thaw rate (2。 to 5$^{\circ}C$) has been used regardless of the freeze rate. 9. To maintain a constant high level of fertility throughout a breeding season with frozen semen, a higher absolute number of spermatozoa must be inseminated (2 to 3 times as many) as compared to fresh semen since a, pp.oximately 50% are destroyed during processing and freezing. 10. The quality of semen may vary with season and age of the male. Such changes in sperm quality could be accentuated by storage effects. Thus, the correct number of spermatozoa may very well vary during the course of a breeding period. 11. As to time of insemination, it is best to avoid inseminating chicken hens within 1-2 hr after or 3-5 hr before oviposition; and turkey hens during or 7-10 hr before oviposition. 12. The physiological receptiveness of the oviduct at the time of insemination is a very important biological factor influencing fertility levels throughout the breeding season.

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