• Journal of Microbiology and Biotechnology
• /
• v.26 no.7
• /
• pp.1173-1181
• /
• 2016

Salmonella spp. are gram-negative flagellated bacteria that cause a variety of diseases in humans and animals, ranging from mild gastroenteritis to severe systemic infection. To explore development of a potent vaccine against Salmonella infections, the gene encoding outer membrane protein L (ompL) was inserted into the swinepox virus (SPV) genome by homologous recombination. PCR, western blot, and immunofluorescence assays were used to verify the recombinant swinepox virus rSPV-OmpL. The immune responses and protection efficacy of rSPV-OmpL were assessed in a mouse model. Forty mice were assigned to four groups, which were immunized with rSPV-OmpL, inactive Salmonella (positive control), wild-type SPV (wtSPV; negative control), or PBS (challenge control), respectively. The OmpL-specific antibody in the rSPV-OmpL-immunized group increased dramatically and continuously over time post-vaccination, and was present at a significantly higher level than in the positive control group (p < 0.05). The concentrations of IFN-γ and IL-4, which represent Th1-type and Th2-type cytokine responses, were significantly higher (p < 0.05) in the rSPV-OmpL-vaccinated group than in the other three groups. After intraperitoneal challenge with a lethal dose of Salmonella typhimurium CVCC542, eight out of ten mice in the rSPV-OmpL-vaccinated group were protected, whereas all the mice in the negative control and challenge control groups died within 3 days. Passive immune protection assays showed that hyperimmune sera against OmpL could provide mice with effective protection against challenge from S. typhimurium. The recombinant swinepox virus rSPV-OmpL might serve as a promising vaccine against Salmonella infection.

• Korean Institute of Interior Design Journal
• /
• v.14 no.3 s.50
• /
• pp.191-198
• /
• 2005

The present study is a preliminary research improving the dwelling quality of one-room type multi-family housings around the university campus. The purpose of the study is to investigate the present condition of Indoor noise level using · residents' responses and field measurements. The respondents are 104 residents living in one-room type multi-family housings. The field measurements on equivalent noise level of indoor and outdoor were carried out in 6 subject house units during the $26th\~28th$ of November 2002. The results are as follows. 1) The residents show relatively non-positive responses at evening and night on the present condition of indoor noise. 2) They answer 'living equipment foise' and 'water hammer' as major types of indoor noise of house unit. 3) Outdoor noise levels, basic factor of noise environment in 6 subject buildings were distributed $52.8\~65.3dB(A)Leq_{5min}$ and were inappropriate to the standard for environmental noise, $55 dB(A)Leq_{5min}$. 4) Indoor noise levels of subject house units were measured as $27.5\~63.5dB(A)Leq_{5min}$, the average of each house unit except one house unit was higher than the level feeling as noise, 40dB(A). 5) It was found that the differences of indoor noise levels between subject house units were caused by 'residents' living noise', 'living equipment noise', 'water hammer', and 'walking and talking noise in stairs and corridors'. 6) Therefore, it is required to plan for improving the quality of noise environment in one-room type multi-family housing around the campus. For example, soundproof construction (including double window with pair glass and balcony), outdoor garden with trees and water for increasing natural sound, interior materials with sound absorbing power to absorb living noise, soundproof pipe or double surface pipe for decreasing 'water hammer', and noiseproof floors, etc. are required.

• IMMUNE NETWORK
• /
• v.13 no.1
• /
• pp.34-41
• /
• 2013

Interleukin-18 (IL-18) has been known to induce interferon-${\gamma}$ (IFN-${\gamma}$) production and promote Th1 immunity. Although mammalian IL-18 has been characterized in great detail, the properties and application of chicken IL-18 remain largely uninvestigated as of yet. In this study, we evaluated the immunomodulatory properties of Salmonella enterica serovar Typhimurium expressing chicken interleukin-18 (chIL-18) on immune responses induced by avian influenza (AI) and Newcastle disease (ND) vaccines. After oral administration of S. enterica serovar Typhimurium expressing chIL-18, chickens were vaccinated intramuscularly with the recommended dose of either inactivated AI H9N2 vaccine or ND (B1 strain) vaccine. Chickens receiving a primary vaccination were boosted using the same protocol 7 days later. Humoral and cell-mediated immune responses were evaluated in terms of HI antibody titers and proliferation and mRNA expression of IFN-${\gamma}$ and IL-4 of peripheral blood mononuclear cells (PBMC) in response to specific antigen stimulation. According to our results, oral administration of S. enterica serovar Typhimurium expressing chIL-18 induced enhanced humoral and Th1-biased cell-mediated immunity against AI and ND vaccines, compared to that of chickens received S. enterica serovar Typhimurium harboring empty vector. Therefore, we conclude that our proposed vaccination regimen using inactivated AI and ND viruses along with oral administration of S. enterica serovar Typhimurium expressing chIL-18 may provide a novel approach in protecting chicken from currently circulating AI and ND virus strains.

• The Korea Journal of Herbology
• /
• v.27 no.6
• /
• pp.123-129
• /
• 2012

Objectives : Allergy has been described as an inflammatory with hypersensitivity resulting from seasonal or perennial responses to specific allergens. The root of Platycodon grandiflorum (Jacq.) A. DC.(Platycodi Radix; Campanulaceae) has been traditionally used to treat chronic diseases such as bronchitis, asthma, pulmonary tuberculosis, inflammation and hyperlipidemia. In this study, we examined the effect of 70% ethanol extract of Platycodi Radix (PR-E) on ovalbumin (OVA)-induced airway inflammation in mice. Methods : Mice were sensitized and challenged by OVA inhalation to induced chronic airway inflammation, and then were intragastrically administered PR-E extract at doses of 50 and 200 mg/kg/day from days 21 to 30 consecutively. The levels of allergic mediators such as histamine, OVA-specific immunoglobulin (Ig) E, and cytokines such as IL-4 and IFN-${\gamma}$ were measured in the sera of mice by ELISA. The histological change of lung tissue was observed by hematoxylin and eosin (H&E) staining. Results : PR-E extract significantly decreased the serum levels of histamine, OVA-specific IgE, and Th2 cytokine, IL-4 compared with those in the OVA-induced group. PE-E extract significantly increased the serum level of Th1 cytokine, IFN-${\gamma}$. Based on lung histopathological studies, inflammatory cell infiltration and mucus hypersecretion were inhibited by PE-E extract administration compared to that in the OVA-induced group. Conclusions : These findings indicate that PE-E extract may be useful as an adjuvant therapy for the treatment of bronchial asthma.

• Human Ecology Research
• /
• v.61 no.4
• /
• pp.673-686
• /
• 2023

The purpose of this study was to identify home economics (HE) teachers' concerns about and perceptions of HE education using the latest technologies in the era of the 4th Industrial Revolution and to reveal whether they differ according to teachers' general background variables. The questionnaire survey method to measure HE teachers' concerns and perceptions of HE education using the latest technologies in the era of the 4th Industrial Revolution was conducted online using the Google Questionnaire from which 150 responses were received. The main results were as follows. Firstly, HE teachers scored an average of 3.46 out of 5 for the latest technology. Among these interests in the latest technology, interest in "augmented reality and virtual reality technologies" scored the highest at an average of 3.80, while interest in "neural network machine learning" (2.78) was low. HE teacher's concerns about HE education using the latest technologies in the era of the 4th Industrial Revolution were high, with an average score of 4.40. Among these concerns for the latest technology, "concern about the results of HE education using the latest technology" scored the highest at 4.53. HE teachers' anxiety about the latest teaching technology in the era of the 4th Industrial Revolution was moderate, averaging 3.05. The highest form of anxiety was "anxiety about the impact on the job" (4.03) and the lowest was fear of "the disappearance of the teacher's job" (2.50). HE teachers' innovation resistance to the latest teaching technology was low at 2.18. Expectations of the latest technology in HE classes in the era of the 4th Industrial Revolution averaged 3.85, slightly higher than the middle of 3.

• Journal of Society of Preventive Korean Medicine
• /
• v.1 no.1
• /
• pp.126-136
• /
• 1997

To procide basic data for improvement of oriental medical care system, the questionnaire interview was carried out with community peoples, over 20 years old, selected randomly in Seoul, Pusan, Taegu and Taejon err. from 10th Mar. to 20th Apr. 1996. The result were summarized as follows: 1. In the general characteristics of subjects, the highest portion of each part was 52.4% of male, 22.4% of thirties, 37.1% of college graduates, 19.1% of married, 30.6% of town dwellers, 63.3% of muddle social class, 26.6% of housewife, 19.3% of student, 16.6% officer and 11.6% of professional technician. 2. The portion of subjects agree to open oriental clinic in western medicine hospital was 60.0%, that of disagreement was 20.1%, Among 60.0% of agreement group, the high rate was showed in female, fifties, middle school graduates, unemployed, middle social level. 3. The rate of positive responses in system of a herb specialist was 64.7%, that of negative responses was 11.6%. Among positive responses, the groups of high rate were male. twenties, above college graduates, student, middle-high social level and city dwellers. 4. In the question whether a pack of herb medicine should be included in medical insurance or not, the rate of agreement was 74.3%, that of disagreement was 4.1%. Amount agreement subjects, the high rate was showed in male, young age, high educated, city dwellers, professional technician middle social class. 5. In the cost of oriental medical care, the portion was divided by 70.3% of expensiveness,25.6% of moderate, 4.1% of low price. among 70.3% of high price, the high rate was in female, forties, agrolivestock-fishery, and town dwellers. 6. In the question what the herb doctor should do for the improvement of oriental medicine, the highest rate was 54.2% in further study. The next was 23.0% in need of western medical and 9.1% in no complain in present situation. Among 54.2% of further study, the high rate was showed in male, forties, high school graduate, profession technician, high social level, christian, married and city dwellers. 7. About institution of oriental care, 86.8% of subjects answered to need of improvement, 8.0% of them replied no problem as present. Amount 86.8% of subjects, the high rate was showed in male, twenties, above college graduate, sales, singles, high social level and city dwellers. 8. About necessity of western medical care instrument in oriental medical hospital, the rate of sightly need was 47.5%, inevitably need was 37.7%, no need was 6.3%, the Positive subjects were showed high rate in male, officer, singer, the younger age, the higher educated, better social level and city dwellers. These results might be useful information for establishing of oriental medical care policies, which open oriental medical clinic in western medical hospital, equip western medical care instrument in oriental medical hospital, improve oriental medical care instrument carry out a herb specialist system, balance the resonable medical care cost and effort for further study & research on oriental medicine to satisfy increasing need of oriental medicine.

• Journal of Korean Elementary Science Education
• /
• v.24 no.2
• /
• pp.111-122
• /
• 2005

The goal of this study was to investigate the differences in the effect of feedback from different providers on elementary school students' science achievement and to examine the responses of the students regarding the feedback. Three 6th grade classes were chosen to be assigned as experimental group 1, experimental group 2, and control group, respectively. In experimental group 1, the teacher provided feedback with students concerning the formative evaluations which were conducted periodically. Experimental group 2 was the one in which students were organized into small groups to compose and exchange feedback with one another. No feedback was of offered to students in the control group. The results of the science achievement test indicated ,that the provision of feedback by the teacher resulted in more gains in student academic achievement. The interviews with students revealed that the learners made positive responses to the feedback provided by the teacher while they showed negative attitudes toward the feedback exchange among students. There were also differences in student responses to feedback between high and low achievers in science. Implications for science lessons and science education research were presented and discussed.

• Korean Journal of Human Ecology
• /
• v.19 no.2
• /
• pp.389-398
• /
• 2010

This study examines the relationship between the concern about well-being lifestyle and the perceived image of Hanji fiber. A survey was conducted among male and female consumers aged 20 and over in Jeonju and Daejon from January 4th to 11th, 2008. A total of 168 responses were analyzed by using descriptive statistics, factor analysis, and ANOVA via the SPSS ver. 12. As a result, four factors were determined in the concern for a well-being lifestyle; product consumption, enjoying culture, using therapy and managing the household. The perceived image of Hanji fiber were classified into four elements; traditional, high-quality, functional and popular images. Among the factors of the concern for a well-being lifestyle and the perceived image of Hanji fibers, a partial relationship was observed. There is a positive relationship, especially between the product consumption factor of a well-being lifestyle and the high-quality and functional image of Hanji fiber. Also, there was a positive relationship between the factor of managing the household and the traditional image of Hanji fiber. However, the therapy interest factor of a well-being lifestyle was negatively related to the traditional image of Hanji fiber.

• Journal of Preventive Medicine and Public Health
• /
• v.11 no.1
• /
• pp.49-58
• /
• 1978

To try to determine the type of medical service wanted by the rural population, in Chungnam Province, a survey amongst the populations of 6 counties was conducted; Within the 6 counties, 2 local communities, which had no access to local medical services, were surveyed. The 12 communities were actively involved in Sae-maul activities, and total number of households surveyed, was 822. The survey was conducted over a 1 month period, from July 16th, 1976, thru August 20th, and the followings are the results, summarised. 1. The largest number of respondents desired a combination of Public Health Center and Country Hospital, followed in order by Private Clinic and Modern Medical Facility. 2. The respondents, aged under 40 years, desired the Private Clinic type medical service, whilst those over 40 years of age, wanted the County Hospital, and as the numbers in this age bracket, were larger, so the ratio was much higher. 3. Sex, educational background, and occupation did not play any particular emphasis in the decisions. 4. Monthly income affected the responses to the survey. These in the lower-income bracket, wanted the County Hospital, and the ratio was high. These in the high income bracket desired the Modern Medical Service, accordingly. Those with an income of 50,000 won or less, amongst the low-income bracket, favored the Public Health Sub-center type of service. The ratio for this service was very high.

• Journal of Korean Elementary Science Education
• /
• v.25 no.3
• /
• pp.231-243
• /
• 2006

The purpose of this study was to investigate misconceptions regarding the structure of flowers and the function of the course based on the 'Flower' section of 5th grade elementary school science courses. It also sought to investigate how misconceptions are changed before and after the application of a Driver learning model, and finally analysing any differences in the correction of misconceptions. A questionnaire was created for 199 5th grade elementary school pupils. The major results before and after using an applied Driver learning mode teaching plan are as follows: In the response for questions, 13.6% and 14.5% of misconceptions were corrected for male and female pupils, respectively. For rural and urban pupils, 14.8% and 11.2% of misconceptions were corrected, respectively. In the comparison of male and female pupils according to the reasons for selection of responses before and after using an applied Driver learning model teaching plan, 27.8% of male and 30.0% of female pupils scientific conceptions showed improvement. For rural and urban schools, 26.6% and 32.2% of scientific conceptions were improved, respectively. Data from this study may help teachers to reconsider their own conceptions regarding the study of the flower as it is presently conducted in elementary school.