The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of Platycodi Radix ethanol extract on ovalbumin-induced allergic responses in mice

난알부민 유도 알레르기 면역반응에 대한 길경(桔梗)에탄올추출물의 효능 연구

  • Jung, Jin Ki (Dept. of Herbology, College of Oriental Medicine, Dongguk University) ;
  • Kang, Seok Yong (Dept. of Herbology, College of Oriental Medicine, Dongguk University) ;
  • Kim, Jinwoong (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University) ;
  • Lee, Sang Kook (College of Pharmacy, Natural Products Research Institute, Seoul National University) ;
  • Park, Yong-Ki (Dept. of Herbology, College of Oriental Medicine, Dongguk University)
  • 정진기 (동국대학교 한의과대학 본초학교실) ;
  • 강석용 (동국대학교 한의과대학 본초학교실) ;
  • 김진웅 (서울대학교 약학대학 생약학교실) ;
  • 이상국 (서울대학교 약학대학 천연물과학연구실) ;
  • 박용기 (동국대학교 한의과대학 본초학교실)
  • Received : 2012.10.17
  • Accepted : 2012.11.06
  • Published : 2012.11.30
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Abstract

Objectives : Allergy has been described as an inflammatory with hypersensitivity resulting from seasonal or perennial responses to specific allergens. The root of Platycodon grandiflorum (Jacq.) A. DC.(Platycodi Radix; Campanulaceae) has been traditionally used to treat chronic diseases such as bronchitis, asthma, pulmonary tuberculosis, inflammation and hyperlipidemia. In this study, we examined the effect of 70% ethanol extract of Platycodi Radix (PR-E) on ovalbumin (OVA)-induced airway inflammation in mice. Methods : Mice were sensitized and challenged by OVA inhalation to induced chronic airway inflammation, and then were intragastrically administered PR-E extract at doses of 50 and 200 mg/kg/day from days 21 to 30 consecutively. The levels of allergic mediators such as histamine, OVA-specific immunoglobulin (Ig) E, and cytokines such as IL-4 and IFN-${\gamma}$ were measured in the sera of mice by ELISA. The histological change of lung tissue was observed by hematoxylin and eosin (H&E) staining. Results : PR-E extract significantly decreased the serum levels of histamine, OVA-specific IgE, and Th2 cytokine, IL-4 compared with those in the OVA-induced group. PE-E extract significantly increased the serum level of Th1 cytokine, IFN-${\gamma}$. Based on lung histopathological studies, inflammatory cell infiltration and mucus hypersecretion were inhibited by PE-E extract administration compared to that in the OVA-induced group. Conclusions : These findings indicate that PE-E extract may be useful as an adjuvant therapy for the treatment of bronchial asthma.

Keywords

References

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