• Title/Summary/Keyword: Th2 differentiation

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Chorion Gene Expression in the Cellular Differentiation and Accumulation of Chorion Protein of Silkmoth, Bombyx mandarina I. Specific Structures of Egg-shell and Chorion Protein (한국산 멧누에 (Bombyx mandarina)에 있어서 난각유전자의 형질발현. I. 난각구조의 특이성과 Chorion 단백질)

  • 노시갑
    • Korean journal of applied entomology
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    • v.29 no.3
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    • pp.157-164
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    • 1990
  • The surface patterns and the structures of transverse section of the egg-shell of the sikmoth, Bombyx mandarina, have been described by scanning electron microscope. Three spatially differentiated cross section, called lamellar, conic pillar and cover layers, are found on the mature eg-shell. Silkmoth chorion proteins were detected more than 80 components from a single chorion by two-dimensional electrophoresis. Major protein components of the egg-shell have bee identified on the basis of their isoelectric points and molecular weights, pH 4-6 and 6-30 kd. Several protein components are found entirely or predominantly in th cover layers.

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The Relationship among Internal Marketing, Satisfaction and Organizational Commitment: Moderating Effect of Economic Performance Change of Business

  • KIM, Boine
    • East Asian Journal of Business Economics (EAJBE)
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    • v.9 no.2
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    • pp.31-42
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    • 2021
  • Purpose- This research's purpose is to give implications on how to increase employee satisfaction and organizational commitment of Korean employees using internal marketing. To do that this study analyzes relationships among variables and analyses mediating effect of satisfaction. Also, this study wants to analyze economic performance change of business as a moderator so group comparison between an economic slump and economic recovery is included. Research design, data, and methodology- To analyze relationships among internal marketing, employee satisfaction and organizational commitment of Korean employees, this study used the 7th KRIVET's HCCP data of employee and firm's financial statements data. And conducted CFA, SEM and group comparison analysis by using AMOS 21. Result- According to the empirical results, employee satisfaction mediates between internal marketing and organizational commitment. However, trust was the only significant antecedent that give a positive influence on employee satisfaction. And employee satisfaction increases organizational commitment. Also, economic performance change of business does significantly deteriorate relationships. Conclusion- Based on this study result, implications to manage the organizational commitment of Korean employees are discussed. Especially, the effect of employee satisfaction and trust discussed. Also, economic performance group which is an economic slump and economic recovery differentiation is highlighted. Therefore the cautious approach is needed in managing employee attitude to job and organization is needed

Effect of Anti-Inflammation through Creation of Skin Fat Barrier on Scutellaria baicalensis extract (황금추출물의 지방장벽 생성을 통한 염증완화 효과)

  • Ahn, Sang Hyun;Kim, Ki Bong
    • The Journal of Pediatrics of Korean Medicine
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    • v.35 no.1
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    • pp.40-47
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    • 2021
  • Objectives The purpose of this study is to confirm the effect of alleviating inflammation through creating skin fat barrier from Scutellaria baicalensis extract. Methods Four-week-old Balb/C mice were divided into four groups: control group (Ctrl), lipid barrier eliminated group (LBEG), dexamethasone (DM) treated group after lipid barrier elimination (DMTG), and Scutellaria baicalensis (SB) treated group after lipid barrier elimination (SBTG). Scutellaria baicalensis extract were administered for 5 days after removal of the fat barrier. Changes in skin condition, improvement of the fat barrier, and relief of inflammation were observed in each group. Results Compared to LBEG and DMTG, pathological skin damage and tissue changes were less in SBTG, and transepidermal water loss (TEWL) and pH were also significantly reduced. Filaggrin was also significantly increased in SBTG. KLK7, PAR-2, and TSLP in SBTG also showed significant reduction compared to the LBEG and DMTG. Conclusions Scutellaria baicalensis extract restores skin barrier and relieves inflammation through the creation of skin fat barrier. This means that the Scutellaria baicalensis extract can regulate Th2 differentiation through the creation of the epithelial fat barrier.

Outcome and Antecedent of Organizational Effectiveness: Moderation Effect of Strategy

  • KIM, Boine
    • East Asian Journal of Business Economics (EAJBE)
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    • v.10 no.2
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    • pp.1-17
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    • 2022
  • Purpose - This exploratory study analyzes the performance of the business from the HRM point of view. Analyzed the influence of HRM and organization effectiveness on business performance. Also moderating effect of the strategy is analyzed. Research design, data, and methodology - Two outcome variables are measured, non-financial performance and financial performance. Two organizational effectiveness variables are measured, job satisfaction and organizational commitment. And two HRM areas are measured, HRM practice and organizational culture. Finally, two strategies are measured as moderators, market strategy and product/service strategy. This study analyzed employees of 554 from the 7th HCCP. This study used SEM and MCFA. Result - The results of turnover intention, growth rate, and profit rate verified the influence of HRM and organizational effectiveness. Overall results showed a contrary effect of education & training system implement and participation on job satisfaction and organizational commitment. Among organizational culture, innovation and relation positively affect job satisfaction and organizational commitment. However, antecedents influencing turnover intention, growth rate, and profit rate bear no resemblance. And the MCFA result shows that strategy's group differentiation analysis is meaningful in turnover intention not in financial performance. Conclusion - Based on this study result, management suggestions for turnover intention, growth rate, profit rate, and organizational effectiveness are made. Especially, due to the company's strategy.

Component Analysis of Cultivated Ginseng, Red Ginseng, Cultivated Wild Ginseng, and Red Wild Ginseng Using HPLC Method (HPLC를 이용한 인삼, 홍삼, 산양산삼 및 홍산삼의 성분 비교 분석)

  • Lee, Jang-Ho;Kwon, Ki-Rok;Cha, Bae-Chun
    • Journal of Pharmacopuncture
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    • v.11 no.2
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    • pp.87-95
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    • 2008
  • Objectives The aim of this experiment is to provide an differentiation of ginseng, red ginseng, cultivated wild ginseng(CWG), and red wild ginseng(RWG) through component analysis using HPLC(High Performance Liquid Chromatography, hereafter HPLC). Methods Comparative analyses of ginsenoside $Rg_3$, ginsenoside $Rh_2$, and ginsenosides $Rb_1$ and $Rg_1$ of various ginsengs were conducted using HPLC. Results 1. CWG was relatively heat-resistant and showed slow change in color during the process of steaming and drying, compared to cultivated ginseng. 2. Ginsenoside $Rg_3$ was not detected in cultivated ginseng and CWG, whereas it was high in red ginseng and RWG. Ginsenoside $Rg_3$ was more generated in red ginseng than in RWG. 3. Ginsenoside $Rh_2$ appreared during steaming and drying of cultivated ginseng, whereas it was more increased during steaming and drying of CWG. 4. Ginsenoside $Rg_1$ content was more increased during steaming and drying of cultivated ginseng, whereas it was more decreased during steaming and drying of CWG. 5. Ginsenoside $Rb_1$ content was increased about 500% during steaming and drying of cultivated ginseng, whereas it was increased about 30% during steaming and drying of CWG, indicating that ginsenoside $Rb_1$ was more generated in red ginseng than in RWG. 6. Ginsenoside $Rg_3$ content was higher, whereas ginsenoside $Rg_1$ content was lower in 11th RWG than in 9th RWG, indicating that ginsenoside $Rg_3$ content was increased and $Rg_1$ content was decreased as steaming and drying continued to proceed. Ginsenoside $Rh_2$ and $Rb_1$ contents began to be increased, followed by decreased after 9th steaming and drying process. Conclusions Above experiment data can be an important indicator for the dentification of ginseng, red ginseng, CWG, and RWG. And the following studies will be need for making good product using CWG.

Changes and characteristics of the biochemical components on the differentiation of soybean cell tissue cultures: (1) Changes and characteristics of the proteins, amino acids and peroxidase isozymes on differentiation of soybean cell tissue cultures (대두 기내 배양체의 분화에 대한 생화학적 성분의 변화와 특성 : (I) 대두 기내 배양체의 분화에 대한 단백질, 아미노산 및 peroxidase 동위효소의 변화와 특성)

  • Nam, Sang-Hae;Choi, Sang-Uk;Yang, Min-Suk
    • Applied Biological Chemistry
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    • v.34 no.2
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    • pp.134-141
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    • 1991
  • In order to investigate the changes and characteristics of biochemical metabolic substances of soybean tissue culture during the cultural period, immature cotyledons were detached form the plant on 15th days after flowering and cultured in vitro for 3 weeks. The cultures were classified into embryogenic(EC) and non-embryogenic callus(NEC). A part of the EC lines were subcultured for another 3 weeks and classified into root forming(RFC), and shoot forming cultures(SFC). Another part of the EC lines were used for isolation of protoplasts, which were subsequently cultured in vitro for 4 weeks. The cultures were classified into embryogenic(PEC) and non-embryogenic callus(PNEC) derived from the protoplasts. The cultures of EC and PEC lines showed higher phenylalanine content and lower methionine content than those of NEC and PNEC. At organ differentiation stage, both cultures showed the content of aspartic acid decreased, while the other amino acids increased as a whole. The protein pattern analysis of the cultures revealed that EC and NEC lines contained distinctive polypeptides, with mass of ca. 18KD for EC and ca. 22KD for NEC respectively. The EC and PEC lines also showed high activity of peroxidase isozyme A(piA), while the RFC and SFC lines showed that of peroxidase isozyme B(piB).

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Effects of the Father's Experience from His Family-of-Origin, Marital Conflict, and Childrearing Behaviors on His Child's Behavioral Problems as a Function of the Child's Sex (아버지의 원가족 분화 경험, 부부갈등, 양육행동이 자녀의 행동문제에 미치는 영향의 모형 검증)

  • Chung, Moon-Ja;Chun, Yeun-Jin
    • Journal of the Korean Home Economics Association
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    • v.43 no.2
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    • pp.41-55
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    • 2005
  • The purpose of this study was to determine the effects of the father's experiences from his family-of-origin, marital conflict, and childrearing behaviors on his child's behavioral problems, as well as any differences due to the child's sex. The subjects were 837 days of 4th and 5th graders in six elementary schools and their fathers in Seoul and Pusan. The data were analyzed using the Structural Equation Modeling(SEM) to verify the theoretical models among the variables. The results showed that the pathways of the impact of the father's experiences from his family-of-origin differed, depending on the father-son group and the father-daughter group, upon the child's intermailizing and extenalizing behavioral problems through marital conflict and the father's childrearing behaviors. Firstly, in the father-son model, the father's experiences from his family-of-origin had an impact on the son's internalizing and extenalizing behavioral problems through marital conflict and the his negative childrearing behaviors. Secondly, the father-daughter model exhibited two pathways; the father's experiences from his family-of-origin had an impact on the child's internalizing and externalizing behavioral problems through his negative chilrearing behaviors, and the father's experiences from family-of-origin had an impact on the child's internalizing behavioral problems through marital conflict and his positive childrearing.

The Effects of Hand Reflexology on Saeng-Chi and Immunity in ESRD Patients (손 반사요법이 말기 신부전증 환자의 생기와 면역 반응에 미치는 영향)

  • Oh Sei-Young
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.9 no.2
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    • pp.213-225
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    • 2002
  • The purpose of this study was to identify the Effect of Hand Reflexology on Saeng-chi of physiologic, emotional & motivational responses and Immunity in ESRD patients who received hemodialysis in two general hospital from June to September. 2001 A two group quasi-experimental research with pre and post test design was used. The number of participants in the experimental group was 23, and in the control group, 20 The Hand Reflexology Intervention was developed by the researcher based on hand reflexology by Carter & Weber and Chi-massage by Chia. The Hand Reflexology was applied to both hands for 10 minutes per day. and 5 days by 5 times. To evaluate the effects of the program, Pre and Post evaluations were done. In the physiologic response, the PR was decreased at the 1st times post treatment and at the 5th. In addition BP was decreased at the 1st time, but not the 5th. After 5 treatments, there were significant increase in Hb and significant decreases in the BUN and Cr. levels in the experimental group. In the emotional and motivational responses, there were significant increases in vigor, mood, uplifts and self care agency scores in the experimental group, but there was no significant difference in the Rosenberg's self esteem score. In the experimental group, significantly increased CD4, and h/s ratios were found, also NK cells were significantly decreased, and there was a decrease in the CD8. However, no significant differences between groups were observed. There were significant increases in CD32, CD33, CD34 in the experimental group. The self care agency score correlated negatively with the CD8. From the above results, Hand Reflexology is shown to be an effective mind-body nursing intervention for enhancing Saeng-chi responses and affecting some of the immune responses. However, Immune cell activation and differentiation with hand reflexology will be achieved with future study.

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Molecular Cloning and Expression of Grass Carp MyoD in Yeast Pichia pastoris

  • Wang, Lixin;Bai, Junjie;Luo, Jianren;Chen, Hong;Ye, Xing;Jian, Qing;Lao, Haihua
    • BMB Reports
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    • v.40 no.1
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    • pp.22-28
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    • 2007
  • MyoD, expressed in skeletal muscle lineages of vertebrate embryo, is one of muscle-specific basic helix-loop-helix (bHLH) transcription factors, which plays a key role in the determination and differentiation of all skeletal muscle lineages. In this study, a cDNA of grass carp MyoD was cloned and characterized from total RNA of grass carp embryos by RT-PCR. The full-length cDNA of grass carp MyoD is 1597 bp. The cDNA sequence analysis reveals an open reading frame of 825 bp coding for a protein of 275 amino acids, which includes a bHLH domain composed of basic domain (1-84th amino acids) and HLH domain (98-142th amino acids), without signal peptide. Then the MyoD cDNA of grass carp was cloned to yeast expression vector pPICZ$\alpha$A and transformed into P. pastoris GS115 strain, the recombinant MyoD protein with a molecular weight of about 31KD was obtained after inducing for 2d with 0.5% methanol in pH 8.0 BMGY medium, and the maximum yield was about 250 mg/L in shaking-flask fermentation. The results were expected to benefit for further studies on the crystal structure and physiological function of fish MyoD.

THE EFFECTS OF IRRADIATION AND CALCIUM-DEFICIENT DIET ON DENTIN AND CEMENTUM FORMATION OF RAT MOLAR (방사선조사와 저칼슘식이가 백서구치의 상아질과 백악질형성에 미치는 영향에 관한 실험적 연구)

  • Shin Jae-Chang;Hwang Eui-Hwan;Lee Sang-Rae
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.28 no.1
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    • pp.87-109
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    • 1998
  • The present study was designed to elucidate the effects of the Co-60 γ irradiation and/or calcium­deficient diet on the dentin and cementum formation of rat molar. The pregnant three-week old Sprague­Dawley rats were used for the study. The experimental group was divided into two groups, irradiation/normal diet group and irradiation/calcium-deficient diet group. The control group was non­irradiation/normal diet group. The abdomen of the rats at the 19th day of pregnancy were irradiated with single absorbed dose of 350cGy. The rat pups were sacrificed on the 14th day after delivery and the maxillae including molar tooth germ were taken. The specimens including the 1st molar tooth germ were prepared to make tissue sections for light and transmission electron microscopy. Some of tissue sections for light microscopy were stained immunohistochemically with anti-fibronectin antibody. The results were as follows; 1. The Hertwig's epithelial root sheath cells, which are related to the differentiation of the tooth-forming cells, showed irregular cellular arrangement, decrease of intercellular junctional complex, and decreased immunoreactivity to the fibronectin after irradiation. These were more severe in the irradiation/calcium-deficient diet group. 2. The cementoblasts at the cementum-forming area showed chromatin clumpings after irradiation. The immu noreactivity to the fibronectin was weaken after irradiation, especially irradiation/calcium-deficient diet group. 3. The odontoblasts at the dentin-forming area showed increase of lysosomes in the cytoplasm and destruction of intercellular junctional complex. The irradiation/calcium-deficient diet group showed decrease of number and density of the electron dense particles and a large number of vacuoles scattered in the dentin matrix. The immunoreactivity was weaken.

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