• Biomolecules & Therapeutics
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• v.20 no.2
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• pp.177-182
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• 2012

Tetrazolium violet is a tetrazolium salt and has been proposed as an antitumor agent. In this study, we reported for the first time that tetrazolium violet not only inhibited human lung cancer A549 cell proliferation but also induced apoptosis and blocked cell cycle progression in the G1 phase. The results showed that tetrazolium violet significantly decreased the viability of A549 cells at $5-15{\mu}M$. Tetrazolium violet -induced apoptosis in A549 cells was confirmed by H33258 staining assay. In A549, tetrazolium violet blocked the progression of the cell cycle at G1 phase by inducing p53 expression and further up-regulating p21/WAF1 expression. In addition, an enhancement in Fas/APO-1 and its two forms of ligands, membrane-bound Fas ligand (mFasL) and soluble Fas ligand (sFasL), as well as caspase, were responsible for the apoptotic effect induced by tetrazolium violet. The conclusion of this study is that tetrazolium violet induced p53 expression which caused cell cycle arrest and apoptosis. These findings suggest that tetrazolium violet has strong potential for development as an agent for treatment lung cancer.

• Journal of Korean Society of Forest Science
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• v.26 no.1
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• pp.23-30
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• 1975

To elucidate the dependability of seed variability determination by tetrazolium, 15 tree species were checked. For the comparison of potential germinability for these tree seeds, x-ray photography and routine germination test in a incubator were implemented. Though there were considerable differences of seed variability in percentage between germination test and tetrazolium test, the judgement by tetrazolium test and x-ray test gave similar results. These findings indicated that tetrazolium test is a reliable method if treated and inferred properly. Solution of 0.25 percent tetrazolium could give a complete colour reaction as well as 0.50 percent solution.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 1996.06a
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• pp.230-232
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• 1996

• Korean Journal of Veterinary Service
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• v.16 no.1
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• pp.34-40
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• 1993

The present study was conducted to investigate the isolation frequency, biochemical prop erties and antimicrobial susceptibility of B. bronchiseptica isolated from slaughtered pigs during the period from March to December, 1992. In Kyeonggi province. A serological survey for antibody of B. bronchiseptica in 200 slaughtered pigs was carried out by agglutination and tetrazolium reduction methods. The results were summarized as follows ; 1. From 80 slaughtered pigs, 27(33.8%) case were isolated and all isolate strains were resistant to Penicillin, Streptomycin, Chloramphenicol, Tetracycline and Ampicllin, while the majority of them were susceptible to Gentamicin, Cloxacin, Colistin, Neomycin, and Kanamycin. 2. Incidence of B. bronchiseptica antibody in 200 slaughtered pigs were measured by agglutination and tetrazolium reduction methods. Agglutination method was shown 38 (19%) of 200 with a titer of below 1：20 and 20(10%) of 200 with a titer of above 1：640. Tetrazolium reduction method was observed 33(16.5%) of 200 with a titer of below 1 : 20 and 32(15%) of 200 with a titer of above 1：640. 3. LSD analysis indicated that the difference of the responses between agglutination test and tetrazolium reduction test was not significant.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.15 no.1
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• pp.37-50
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• 1989

The in Vitro chemosensitivity of fibroblast cell strains was determined using a semiautomated tetrazolium-based colorimetric assay(MTT assay) to 16 cosmetic materials. This assay is useful method to evaluate toxic effects of the chemicals. From assay results, we determined that the preservatives are more toxic than moisteurizers. The chemicals in the same group have a different toxicity. That is, in preservatives, Germall -115 is more toxic than Danisol -M, -p, and in surfactant sodium laurel sulfate than Myrj 52, and in moisteurizers, 1, 3-butylene glycol is more safe than the others. When the results from this assay for preservatives were compared with patch test results, good correlation was observed. Forthemore, this assay method can be used together with Patch test for the evaluation of the chemical toxicity, particularly in cosmetic field.

• Journal of Plant Biology
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• v.18 no.4
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• pp.150-154
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• 1975

Four kinds of Nicotiana species and five varieties belonging to N. tabacum were used as materials for electrophoretic analysis of the tetrazolium oxidase isozyme to examine the taxonomic affinity among them based on the biochemical property. All the five verieties of N. tabacum showed same isozyme bands despite the fact that these varieties had notably varied characteristics including morphological traits. The band patterns were more or less different among the four species. Although N. rustica and N. tabacum were of the same genome group of AABB, their isozyme bands showed considerable difference. N. sylvestris, genome A donor of Nicotiana species, was found to be markedly different from N. tatacum in band pattern, including the absence of system 2 in N. sylvestris.

• Natural Product Sciences
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• v.26 no.4
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• pp.334-339
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• 2020

A new compound, 3��-acetoxylanosta-7,9(11),24-triene-26-al (3), and seven known compounds (1 - 2 and 4 - 8) were isolated from Ganoderma tropicum (Jung.) Bres. collected in Tay Nguyen, Vietnam. The structures of these compounds were determined by one- and two-dimensional nuclear magnetic resonance spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and high-resolution ESI-MS, and by comparison with literature data. All of the isolated compounds were tested for nitroblue tetrazolium (NBT) reduction activity in Saccharomyces cerevisiae-stimulated RAW 246.7 cells. Among them, compounds 2 - 4 and 6 - 8 enhanced the NBT reduction in a dose-dependent manner.

• Journal of fish pathology
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• v.28 no.2
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• pp.71-78
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• 2015

In this study, we conducted to the development of a rapid antimicrobial susceptibility test method using WST-1 which is known to water-soluble tetrazolium salt, in order to rapidly response against bacterial diseases in fish. Eight of antibiotics which are permissioned for marine organism from government were used to rapid antimicrobial susceptibility testing using the WST-1. As a result, a similar tendency was verified compare to conventional antibiotic susceptibility test results. Generally, the antibiotic susceptibility test method required about 3 days (72 hours) for determine the effective antibiotics, however, we have confirmed that the our method using WST-1 was required at least 36 hours in this study. Consequentially, our method will contribute to development of rapid antimicrobial susceptibility testing for bacterial diseases in fish.

• Journal of the Korean Society of Tobacco Science
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• v.2 no.2
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• pp.38-43
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• 1980

The spectrophotometric determination oi reducing sugar in the tobacco leaves was carried out by employing blue tetrazolium chloride as a color developing reagent. The absorption maximum of formazan dye which is the product of the reaction between blue tetrazolium chloride and fructose was shown to be $530^{nm}$. In the fructose concentration range of 0.02mg/ml-0.14mg/ml the calibration curve was well coincided to the law of Beer-Lambert. In order to take a look at the accuracy and/or recovery rate of fructose determination, the standard fructose was added to the tobacco leaves and the concentration of this standard fructose was estimated. A tittle lower concentration of the standard fructose compared with the pure one in solution was observed. However, an excellent analytical recovery was revealed under the -2% of relative error limit. When we carried out the quantitative determination of this reducing sugar by the method of visual read-out (without using the spectrophotometer), the relative error was obtained to be $\pm$10%.

• Korean Journal of Food Science and Technology
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• v.54 no.1
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• pp.1-7
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• 2022

Riboflavin (Rb), in the presence of methionine (Met) under light, generates superoxide radicals that can reduce nitro blue tetrazolium (NBT) to its corresponding formazan. The Rb-Met/NBT system has been used to measure the superoxide dismutase (SOD)-like activities of various antioxidants. However, the reaction mechanisms have not been clearly defined, and the assay conditions are not consistent. In this study, the effects of different photosensitizers and amino acids on NBT reduction in different solvents were investigated. NBT reduction in the Rb-Met/NBT system was more pronounced in phosphate-buffered saline, compared to distilled water or Tris (pH 7.5); histidine (His) instead of Met also led to considerable Rb-induced NBT reduction. Among the photosensitizers, methylene blue with His caused potent NBT reduction in Tris. Rb-induced NBT reduction combined with Met or His was quantitatively inhibited by SOD or gallic acid, but did not affect MB-induced reduction sensitively.