• ALGAE
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• 제24권4호
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• pp.205-212
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• 2009

Molecular genetic tools are widely used to learn more about the identical characterization of obscure microalgal strains. At the Korea Marine Microalgae Culture Center (KMMCC), the authors deduced the genetic relationship of 41 strains of the genus Tetraselmis by analysing a small subunit ribosomal DNA (18S rDNA) sequences. Forty-one strains were seperated into five groups, which showed over a 98-99% similarity to Tetraselmis striata or Tetraselmis sp. Tsbre. Also, 13 strains among them had an identical genotype to Tetraselmis striata while 5 strains had with Tetraselmis sp. Tsbre, respectively. The mean size of each strain generally showed the tendency of different variation according to the groups.

• 한국수산과학회지
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• 제51권1호
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• pp.64-71
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• 2018

This study was conducted to investigate the effects of light intensity and wavelength on the growth of Tetraselmis suecica and Tetraselmis tetrathele. These species were exposed to a blue light-emitting diode (LED; max=450 nm), a yellow LED (max=590 nm), a red LED (max=630 nm) and a fluorescent lamp (three wavelengths). The maximum growth rates (${\mu}_{max}$) of T. suecica and T. tetrathele under a red LED were 1.12/day and 0.95/day, respectively. Under a yellow LED, growth rates were 70% of the values for red wavelength, with low half-saturation constants (Ks). The optimum light source to ensure economically effective and productive growth in a Tetraselmis culture system (Photo-Bioreactor) would thus appear to be a three-phase culture, wherein a yellow LED is used during the lag phase and initial exponential phase to increase growth rate, followed by a red LED during the middle exponential phase to maximize growth rate, and finally a yellow LED again during the late exponential phase and stationary phase to achieve increased yield of useful bioactive substances.

• 한국양식학회지
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• 제11권2호
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• pp.231-240
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• 1998

온도와 염분에 대하여 내성이 강하고 대량배양이 쉬워서 조개류의 인공종묘생산시 먹이로서 많이 사용하고 있는 5종의 Tetraselmis (T. tetrathele, T. suecica, T. subcordiformis, T. sp. (Haeundae), T. sp (China)) 중 우리나라 기후 환경에 대향배양이 가장 적합한 종을 선택하기 위핵서 최적 배양환경 (온도, 염분)을 조사하고 이때의 세포크기와 영양성분을 조사하였다. 5종의 Tetraselmis의 세포크기 및 체적은 T. sp. (Haeundea)가 장경 $17.6{\pm}1.87$${\mu}$m, 체적 727${\mu}$$m^3$로 가장 대형종이었으며 중국산 T. sp.가 장경14.6+- 1.46${\mu}$m, 체적 625${\mu}$$m^3$으로 가장 소형종이였다. Tetraselmis는 광염성, 광온성이나 최적성장을 위한 수온과 염분 구간은 각각 24~$30^{\circ}C.$와 27~30%.였다. 5종의 Tetraselmis에서 고수온에 대한 내성은 중국산 Tetraselmis, 저수온데 대한 내성은 T. tetrathele가 높은 것으로 나타났다. 5종의 Tetraselmis중 배양밀도가 가장 높았던 종은 T. suecica였으며, 이 종은 배양 7일째 $24^{\circ}C.$, 30%.에서 세포수가 $141{\times}10^4$cells/ml (s.g.r., 0.3906)로 성장이 가장 좋았다. 5종의 Tetraselmis의 화학성분중 조단백질 함량은 T. suecica가 44.50%로 가장 높게 나타났고 조지방은 해운대산 T. sp.가 7.13%로 높게 나타났다. 필수아미노산은 해운대산 .T.sp.에서 50.40%로 가장 높게 나타났고, 불포화지방산은 중국산 T.sp.에서 11.7%로 가장 높게 나타났다. 5종의 Tetraselmis의 성장과 영양성분 결과로 보아 우리나라에서 대량배양이 가장 적합한 종은 T. seucica임을 알수 있었다.

• 해양환경안전학회지
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• 제25권1호
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• pp.96-101
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• 2019

본 연구는 담녹조강 Tetraselmis suecica와 T. tetrathele의 인산염 흡수에 미치는 발광다이오드의 파장의 영향을 조사하였다. 파장은 청색 발광다이오드(LED; 450 nm), 황색 LED(590 nm), 적색 LED(630 nm) 그리고 형광램프(control)이며, 조도는 $100{\mu}mol\;m^{-2}\;s^{-1}$로 주사하였다. T. suecica와 T. tetrathele의 최대흡수속도(${\rho}_{max}$)는 적색 LED에서 $6.35pmol\;cell^{-1}\;hr^{-1}$와 $9.85pmol\;cell^{-1}\;hr^{-1}$로 나타났으며, 반포화농도(Ks)는 $9.43{\mu}M$와 $21.2{\mu}M$로 나타났다. 이는 적색 LED 아래에서 T. suecica와 T. tetrathele가 다른 파장보다 영양염에 대한 친화성이 높다는 것으로 의미한다. 따라서 경제성 및 생산성 향상을 위한 Tetraselmis 배양시스템(광배양기)의 최적 광원은 낮은 영양염 상태에서도 높은 생장속도를 보이는 적색 LED가 적합할 것으로 판단된다.

• 한국수산과학회지
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• 제44권1호
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• pp.1-7
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• 2011

This study examined Tetraselmis sp. JK-46 isolated from seawater from the East Sea. Deep seawater (DSW) had a greater effect on the growth of Tetraselmis sp. JK-46 than surface seawater (SSW). The crude protein, lipid, carbohydrate and ash contents of Tetraselmis sp. JK-46 cultured with DSW were 27.2, 37.1, 13.2 and 26.3 %, respectively, and these values were similar to the results for samples cultured with SSW. The contents of Mg, Ca, Fe and K in the DSW cultured samples were 7080.3, 1009.6, 251.2, and 2749.7 mg/100 g, respectively. The fatty acid compositions of Tetraselmis sp. JK-46 cultured with DSW and SSW were 53.7 and 49.0 % polyunsaturated fatty acids (PUFA) and 25.7 and 30.7 % saturated fatty acids (SFA), respectively. The total amino acid contents of the samples cultured with DSW and SSW were 7392.6 and 6376.0 mg/100 g respectively. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of Tetraselmis sp. JK-46 extracts increased with the concentration of the chloroform and ethyl acetate fractions. The half maximal inhibitiory concentrations ($IC_{50}$) of the chloroform and ethyl acetate fractions of DSW and SSW cultured samples were 1.2 and 2.6 mg/mL, and 3.1 and 3.3 mg/mL, respectively. The ethyl acetate fractions of DSW and SSW cultured samples has anticoagulant activity and the activated partial thromboplastin times (APTT) were 93.4 and 89.3 sec., respectively. The chloroform and ethyl acetate fractions showed antimicrobial activity against Bacillus subtilis, Escherichia coli and Candida albicans.

• Journal of Microbiology and Biotechnology
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• 제26권6호
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• pp.1098-1102
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• 2016

A green microalga, Tetraselmis sp., was cultivated in the coastal seawater of Young-Heung Island using semi-permeable membrane photobioreactors (SPM-PBRs) in different seasons. The microalgae in the SPM-PBRs were able to grow on nutrients diffused into the PBRs from the surrounding seawater through SPMs. The biomass productivity varied depending on the ion permeabilities of the SPMs and environmental conditions, whereas the quality and quantity of fatty acids were constant. The temperature of seawater had a greater influence than solar radiation did on productivity of Tetraselmis sp. in SPM-PBRs. SPM-PBRs could provide technologies for concurrent algal biomass and fatty acids production, and eutrophication reduction in the ocean.

• 한국해양바이오학회지
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• 제8권1호
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• pp.39-44
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• 2016

The microalgae have been studied for a source of biodiesel production. It is important to select the microalgae, which grows rapidly in local environmental conditions such as temperature range and ingredient of local seawater. The aim of this study was isolating microalga, which has rapid growth rate and high FAME contents in wide temperature ranges, for microalgal offshore cultivation in Korea, one of the country with four distinct seasons. Firstly, we had isolated a green microalga, Tetraselmis sp. KCTC12236BP, which has faster growth rate in low temperature (5 and $10^{\circ}C$) than Tetraselmis suecica and Dunaliella tertiolecta LB999 from Young Heung Island, Incheon, Korea. This microalga was cultivated in outdoor circulated tank photobioreactor (CT-PBR). As a result, this microalga could grow in wide temperature ranges (6 to $29^{\circ}C$), outdoors. After that, the biomass was recovered, and 13.2 g biodiesel could be acquired from 110 g dry biomass. These results indicate that the isolated microalga, Tetraselmis sp. KCTC12236BP is proper to biodiesel production using outdoor cultivation in Korea for all seasons.

• 한국해양바이오학회지
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• 제11권1호
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• pp.23-28
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• 2019

For successful microalgal biodiesel production, the strain should be selected carefully. Fast growth rate and high fatty acid contents are desired traits for algal biodiesel production. In ocean cultivation of microalgae, seawater temperature slowly changes over seasons, and rotating algal strains in accordance with their optimal temperature could improve overall productivity. Additionally, use of indigenous strain is preferred to alleviate potential impacts on the environment. In this study, five strains of Tetraselmis sp. from nearshore of Youngheung Island, Incheon, Korea, were isolated during winter and characterized for their growth patterns and fatty acid compositions in the low temperatures ($5-15^{\circ}C$). The five strains showed various characteristics in optimal growth temperature, fatty acid contents, and compositions. Compared with a strain of Tetraselmis sp., isolated from Ganghwa island in a previous study, a rapid-growing strain with 237% higher biomass productivity and an oleaginous strain with twice higher fatty acid contents at $10^{\circ}C$ were isolated. The oleaginous Tetraselmis strain showed the highest fatty acid productivity among the strains, having 438% higher productivity than the previous strain. Using the new isolates in the seasons with low seawater temperature would improve microalgal fatty acid productivity in ocean cultivation.

• 해양환경안전학회지
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• 제29권1호
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• pp.36-43
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• 2023

본 연구는 Tetraselmis suecica와 T. tetrathele의 영양물질 증진효과를 가져올 수 있는 배양시스템 구축을 위하여, 발광다이오드(LED)의 파장별(청색; 450 nm, 황색; 590 nm, 적색; 630 nm) 탄수화물, 단백질, 지질 함량을 측정하였다. 두 종 모두 단백질 비율(42~69%)이 가장 높았으며, 생장속도가 낮았던 황색파장에서 탄수화물, 단백질, 지질의 높은 함량을 보였고, 생장속도가 가장 높았던 적색파장에서는 낮은 함량을 보였다. 이러한 결과는 세포 분열 속도의 감소로 인해 단백질 합성과 함께 세포의 화학적 조성과 효소 활동에 변화를 주어 지질과 탄수화물 함량이 증가한 것으로 생각된다. 따라서, T. suecica와 T. tetrathele의 유용한 생화학적 물질의 증대를 위해 대수생장기 초기와 중기는 적색 LED 그리고 대수생장기 후기에는 황색 LED를 주사하는 2단계 LED 배양을 제안하였다.

• 한국환경과학회지
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• 제21권6호
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• pp.757-761
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• 2012

The purpose of this study was to determine optimum conditions for the cultivation of Tetraselmis suecica (T. suecica) under illumination of four different types of LEDs (i.e., blue, red, white, and mixed). Initial cell concentration was $4{\times}10^4$ cells/mL and temperature of reactor was maintained between 21-$24^{\circ}C$. Specific growth rates were 0.72 $day^{-1}$(white), 0.58 $day^{-1}$(red), 0.49 $day^{-1}$(mixed), and 0.49 $day^{-1}$(blue). Thus, white LEDs was used for the cultivation of T. suecica. Tests with white LEDs under different light intensity, which was conducted to determine optimum light intensity of white LEDs, showed that 9,000 lux of illumination resulted in fastest cell growth and greatest cell concentrations. To avoid shadow effects by dense cell populations, aeration was performed. Cell concentration increased 3.8 times when aeration was used.