Browse > Article
http://dx.doi.org/10.7837/kosomes.2019.25.1.096

The Effects of the Light Quality of a Light Emitting Diode (LED) on the Phosphate Uptake of Prasinophytes Tetraselmis suecica and Tetraselmis tetrathele  

Han, Kyong Ha (Department of Oceanography, Pukyong National University)
Oh, Seok Jin (Department of Oceanography, Pukyong National University)
Publication Information
Journal of the Korean Society of Marine Environment & Safety / v.25, no.1, 2019 , pp. 96-101 More about this Journal
Abstract
This study was conducted to investigate the effects of the light quality of a Light Emitting Diode (LED) on the phosphate uptake of Prasinophytes Tetraselmis suecica and Tetraselmis tetrathele. These species were exposed to a blue LED (max = 450 nm), a yellow LED (max = 590 nm), a red LED (max = 630 nm) and a fluorescent lamp (control) at $100{\mu}mol\;m^{-2}\;s^{-1}$. The maximum uptake rates (${\rho}_{max}$) of T. suecica and T. tetrathele under the red LED were $6.35pmol\;cell^{-1}\;hr^{-1}$ and $9.85pmol\;cell^{-1}\;hr^{-1}$, respectively. The half saturation constants (Ks) of two species were $9.43{\mu}M$ and $21.2{\mu}M$, respectively. The phosphate affinity of the two species under the red LED was higher than that of other wavelengths. Thus, the optimum light source to ensure economically effective and productive growth in a Tetraselmis culture system (Photo-Bioreactor) would be red LEDs because of the high growth rate shown, regardless of relatively low nutrient conditions.
Keywords
Tetraselmis suecica; Tetraselmis tetrathele; Phosphate; Red LED; Maximum Uptake rate; Half Saturation Constant;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Cameron, H., M. T. Mata and C. Riquelme(2018), The effect of heavy metals on the viability of Tetraselmis marina AC16-MESO and an evolution of the potential use of this microalgae in bioremediation, Vol. 6, p. 5295.   DOI
2 Eppley, R. W., J. N. Rogers and J. J. McCarthy(1969), Half saturation constants for uptake of nitrate and ammonium by marine phytoplankton, Limnology Oceanography, Vol. 14, No. 6, pp. 912-920.   DOI
3 Fu, W., O. Guamundsson, G. Paglia, G. Herjolfsson, O. S. Andresson, B. O. Palsson and S. Brynjolfsson(2013), Enhancement of carotenoid biosynthesis in the green microalga Dunaliella salina with light-emitting diodes and adaptive laboratory evolution, Applied microbiology and biotechnology, Vol. 97, No. 6, pp. 2395-2403.   DOI
4 Gomez, P. and M. A. Gonzalez(2004), Genetic variation among seven strains of Dunaliella salina (chlorophyta) with industrial potential, based on RAPD banding patterns and on nuclear ITS rDNA sequences, Aquaculture, Vol. 233, No. 1-4, pp. 149-162.   DOI
5 Han, K. H. and S. J. Oh(2018), Effects of Various Intensities and Wavelengths of Light Emitting Diodes (LEDs) on the Growth of the Prasinophytes Tetraselmis suecica and T. tetrathele, Korean Journal of Fisheries and Aquatic Science, Vol. 51, No. 1, pp. 64-71.   DOI
6 Harrison, P. J., J. S. Parslow and H. L. Conway(1989), Determination of nutrient uptake kinetic parameters: a comparison of methods, Marine Ecology Progress Series, Vol. 52, pp. 301-312.   DOI
7 Ladygina, N., E. G. Dedyukhina and M. B. Vainshtein(2006), A review on microbial synthesis of hydrocarbons, Process Biochemistry, Vol. 41, No. 5, pp. 1001-1014.   DOI
8 Jeon, Y. C., C. W. Cho and Y. S. Yun(2005), Measurement of microalgal photosynthetic activity depending on light intensity and quality, Biochemical Engineering Journal, Vol. 27, No. 2, pp. 127-131.   DOI
9 Kim, C. W. and S. B. Hur(1998), Dietary value of frozen and freeze-dried Tetraselmis suecica, Journal of Aquaculture, Vol. 11, No. 2, pp. 183-191.
10 Kwon, H. K.(2013), A study on phytoremediation of eutrophic coastal sediments using benthic microalgae and light emitting diode, Ph.D Thesis, Pukyong National University, Busan, Korea.
11 Lee, Y. J., C. H. Lee, K. Cho, H. N. Moon, J. Namgung, K. H. Kim, B. J. Lim, D. Kim and I. K. Yeo(2017), Effect of Temperature-induced two-stage cultivation on the lipid and saccharide accumulation of Microalgae Chlorella vulgaris and Dunaliella salina, Korean Journal Aquatic Science, Vol. 50, No. 1, pp. 32-40.   DOI
12 Matthijs, H. C., H. Balke, U. M. van Hes, B. M. Kroon, L. R. Mur and R. A. Binot(1996), Application of light-emitting diodes in bioreactors: flashing light effects and energy economy in algal culture (Chlorella pyrenoidosa), Biotechnology Bioengineering, Vol. 50, No. 1, pp. 98-107.   DOI
13 Oh, S. J., H. K. Kwon, J. Y. Jeon and H. S. Yang(2015), Effect of monochromatic light emitting diode on the growth of four microalgae species (Chlorella vulgaris, Nitzschia sp., Phaeodactylum tricornutum, Skeletonema sp.), Journal of the Korean Society of Marine Environment & Safety, Vol. 21, No. 1, pp. 1-8.   DOI
14 Maddux, W. S. and R. F. Jones(1964), Some interaction of temperature, light intensity and nutrient concentration during the continuous culture of Nitzschia closterium and Tetraselmis sp, Limnology and Oceanography, Vol. 9, No. 1, pp. 79-86.   DOI
15 McHugh, D. J.(2003), A guide to the seaweed industry, Food and Agriculture Organization of the United Nations, Rome, p. 118.
16 Michels, M. H., M. Vaskoska, M. H. Vermue and R. H. Wijffels(2014), Growth of Tetraselmis suecica in a tubular photobioreactor on wastewater from a fish farm, Water Research, Vol. 65, No. 15, pp. 290-296.   DOI
17 Min, B. H.(2018), Growth and survival on live food for larval development stage of Ark shell Scapharca broughtonii, The Korean Journal Malacology, Vol. 34, No. 2, pp. 79-88.   DOI
18 Mouget, J. L., P. Rosa and G. Tremblin(2004), Acclimation of Haslea ostrearia to light of different spectral qualities confirmation of 'chromatic adaptation' in diatoms, Journal of Photochemistry and Photobiology B: Biology, Vol. 75, No. 1-2, pp. 1-11.   DOI
19 Pulz, O. and W. Gross(2004), Valuable products from biotechnology of microalgae, Applied Microbiology Biotechnology, Vol. 65, No. 6, pp. 635-48.   DOI
20 Park, H. J., E. J. Jin, T. M. Jung, H. Joo and J. H. Lee(2010), Optimal culture conditions for photosynthetic microalgae Nannochloropsis oculata, Journal of the Korean Industrial and Engineering Chemistry, Vol. 21, No. 6, pp. 659-663.
21 Schulze, P. S., C. F. M. Carolina, H. Pereira, K. N. Gangadhar, L. M. Schuler, T. F. Santos, J. C. S. Varela and L. Barreira(2017), Urban wastewater treatment by Tetraselmis sp. CTP4 (Chlorophyta), Bioresource Technology, Vol. 223, pp. 175-183.   DOI
22 Parke, M. and J. C. Green(1976), Chlorophyta, Prasinophyceae. In: Parke, M. and P. S. Dixon(eds.) Check-list of British marine algae (third revision), Journal of the Marine Biological Association of the United Kingdom, Vol. 56, pp. 564-566.
23 Porter, K. G. and Y. S. Feig(1980), The use of DAPI for identifying and counting aquatic microflora, Limnology Oceanography, Vol. 25, No. 5, pp. 943-948.   DOI
24 Ra, C. H., C. H. Kang, J. H. Jung, G. T. Jeong and S. K. Kim(2016), Effects of light-emitting diodes (LEDs) on the accumulation of lipid content using a two-phase culture process with three microalgae. Bioresource technology, Vol. 212, pp. 254-261.   DOI
25 Raja, R., S. Hemaiswarya, N. Ashok Kumar, S. Sridhar and R. Rengasamy(2008), A perspective on the biotechnological potential of microalgae. Critical Reviews in Microbiology, Vol. 34, pp. 77-88.   DOI
26 Sanchez-Saavedra, M. P. and D. Voltolina(1994), The chemical composition of Chaetoceros sp. (Bacillariophyceae) under different light conditions. Comparative Biochemistry and Physiology, Vol. 107B, No. 1, pp. 39-44.
27 Spolaore, P., C. Joannis-Cassan, E. Duran and A. Isambert (2006), Commercial applications of microalgae. Journal of Bioscience and Bioengineering, Vol. 101, No. 2, pp. 87-96.   DOI
28 Wang, C. Y., C. C. Fu and Y. C. Liu(2007), Effects of using light-emitting diodes on the cultivation of spirulina platensis. Biochemical Engineering Journal, Vol. 37, pp. 21-25.   DOI
29 Su, C. H., L. J. Chien, J. Gomes, Y. S. Lin, Y. K. Yu, J. S. Liou and R. J. Syu(2011), Factors affecting lipid accumulation by Nannochloropsis oculata in a two-stage cultivation process. Journal Applied Phycology Vol. 23, No. 5, pp. 903-908.   DOI
30 Takano, H., T. Arai, M. Hirano and T. Matsunaga(1995), Effects of intensity and quality of light on phycocyanin production by a marine cyanobacterium Synechococcus sp. NKBG 042902. Applied Microbiology and Biotechnology, Vol. 43, No. 6, pp. 1014-1018.   DOI