• 한국작물학회지
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• 제57권1호
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• pp.71-77
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• 2012

The objective of this study is to develop the gene based sequence tagged site (STS) markers in wheat. The euchromatin enriched genomic library was constructed and the STS primer sets were designed using gene based DNA sequence. The euchromatin enriched genomic (EEG) DNA library in wheat was constructed using the $Mcr$A and $Mcr$BC system in $DH5{\alpha}$ cell. The 2,166 EEG colonies have been constructed by methylated DNA exclusion. Among the colonies, 606 colonies with the size between 400 and 1200 bp of PCR products were selected for sequencing. In order to develop the gene based STS primers, blast analysis comparing between wheat genetic information and rice genome sequence was employed. The 227 STS primers mainly matched on $Triticum$ $aestivum$ (hexaploid), $Triticum$ $turgidum$ (tetraploid), $Aegilops$ (diploid), and other plants. The polymorphisms were detected in PCR products after digestion with restriction enzymes. The eight STS markers that showed 32 polymorphisms in twelve wheat genotypes were developed using 227 STS primers. The STS primers analysis will be useful for generation of informative molecular markers in wheat. Development of gene based STS marker is to identify the genetic function through cloning of target gene and find the new allele of target trait.

• 식물조직배양학회지
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• 제28권2호
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• pp.113-116
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• 2001

성염색체의 조성이 다른 수영 (Rumex acetosa L.) 암·수 개체의 잎 절편 배양을 통하여 얻은 체세포클론을 대상으로 염색체 분석을 수행하여 다음과 같은 결과를 얻었다. 암 개체의 경우 조사된 25개체 중 21개체 (84%)가 야생형과 같은 염색체 조성 (2n=14)을 보였으며, 야생형에서는 관찰되지 않는 4배체 식물이 4개체 (16%)가 관찰되었다. 그에 비하여 수 개체에서는 20개체 중 4개체 (20%)만이 정상의 염색체 조성을 보여 성에 따른 체세포클론 변이에서의 차이를 보였다. 수 개체에서는 2n=14, 28 등의 이수체가 관찰되었으며, 4배체 (2n=30)로 나타난 체세포클론에서도 성염색체의 뚜렷한 배가현상이 관찰되지 않았다.

• 식물분류학회지
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• 제51권1호
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• pp.1-9
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• 2021

Symplocarpus koreanus (Araceae; Orontioideae) from Korea is described as new. Symplocarpus koreanus has long been considered to be conspecific with S. renifolius in Japan, but phylogenetic, morphological, and cytological data indicate its taxonomic distinction. Compared to S. renifolius, S. koreanus has a much smaller spathe and more spherical spadix with fewer, smaller flowers. Previous phylogenetic studies also suggested that S. koreanus is more closely related to nonthermogenic S. nipponicus than to S. renifolius in Japan. Like its nonthermogenic sister species, S. nipponicus, in Korea and Japan, S. koreanus is diploid (2n = 2x = 30), while S. renifolius in Japan is tetraploid (2n = 4x = 60). A detailed species description, geographical distribution, major morphological differences between the species and a dichotomous key to the species in eastern Asia are provided.

• 농업과학연구
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• 제50권3호
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• pp.365-371
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• 2023

This experiment was carried out to breed a medium maturing variety of Italian ryegrass (Lolium multiflorum Lam.) in Grassland and Forage Crops Division, National Institute of Animal Science, Rural Development Administration (RDA), Cheonan from 2019 to 2020. An Italian ryegrass variety, IR 604 is a tetraploid variety with green in leaf color and has half-erect growth habit in late autumn and erect growth habit in early spring, IR 604 was on May 17 in heading date as a medium maturing variety. Also, IR 604 was longer by 11 cm in flag leaf length and longer by 1.3 cm in plant height than those of the control variety, Kowinner, respectively. IR 604 was also thicker by 1.0 mm in stem thickness and strong in winter hardness. Dry matter (DM) yield (11,036 kg/ha) of IR 604 was 16.6% higher than that of Kowinner. Total digestible nutrient (TDN), crude protein (CP) and relative feed value (RFV) of IR 604 were 60.4, 13.8% and 100.6 which are 1.4, 3.3%, 7.7 higher than those of Kowinner, respectively. Acid detergent fiber (ADF) and neutral detergent fiber (NDF) of IR 604 were 35.5 and 56.7% which are 1.3 and 3.6% lower than those of Kowinner, respectively.

• 원예과학기술지
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• 제34권5호
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• pp.761-770
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• 2016

본 연구는 고품질을 가진 노동절감형 3배체 포도의 효율적인 육성 가능성을 검토하기 위해서, 4배체와 2배체의 교배를 통해서 얻어진 3배체 포도계통들에서 과실 특성의 변이 를 교배조합별로 검정하였다. 비록 가용성 고형물 함량과 적정산도와 같은 과실의 품질과 관련 된 형질들은 교배조합들 사이에서 유의미한 차이가 관찰되지 않았지만, $GA_3$ 1회 처리에 따른 과실의 비대 정도는 교배조합에 따른 변이가 크게 나타났다. 특히, '거봉'과 '적령'의 교배 조합에서 생성된 3배체 후대계통에서의 평균 과방중은 491g으로써 다른 교배조합에서 생성된 후대계통들에 비해서 유의적으로 컸으며, 동일한 교배조합 내의 계통간에서의 차이도 크지 않았다. 이러한 연구결과는 '거봉'과 '적령' 같은 특정한 교배 조합이 고품질을 가진 노동력 절감형 대립계 무핵포도를 육성하는데 있어서 효율적으로 이용될 수 있음을 시사하고 있다

• Reproductive and Developmental Biology
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• 제33권2호
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• pp.93-98
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• 2009

In this study, bovine Dnmt1 cDNA was sequenced and detected Dnmt1 mRNA level in bovine tissues by northern blot, methylation pattern of genome by southern blot, specific localization of Dnmt1 in mouse and bovine preimplantation embryos by immunocytostaining and Dnmt1 protein level in ovary and testis by western blot. Bovine Dnmt1 cDNA sequence showed more homology with that of human than mouse and rat. The RNA level of Dnmt1 was 10 times higher expression in placenta than other tissues. This indicates that placenta was hypermethylated compared to others organs. The genomic DNA could not be cut by a specific restriction enzyme (HpaII) in placenta, lung and liver of bovine. It suggests that Dnmt1 in some somatic cells was already methylated. Dnmt1, which has the antibody epitope 1316~1616, was distributed in nucleus and cytoplasm including the stage of pronuclear stage and maturation of oocyte and gradually weaken to blastocyst stage compare to negative. In addition, Dnmt1 was strongly expressed in tetraploid embryo and cloned 8-cell than IVF 8-cell. An aberrant pattern of DNA methylation in cloned embryo may be abnormal development of fetus, embryonic lethality and placenta dysfunction. The somatic specific band (190kDa) was appeared in ovary and testis, but oocyte specific band (175kDa) was not. Further investigations are necessary to understand the complex links between the methyltransferases and the transcriptional activity of genes in the cloned bovine tissues.

• 한국잠사곤충학회지
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• 제46권1호
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• pp.12-17
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• 2004

잠상 123호를 수원, 공주, 전주와 장성 4개 지역에서 수행한 지역적응시험 결과, 수량성이 높고 엽질 등이 우수하여 2001년 수일뽕으로 명명하여 장려품종으로 지정하였으며 그 주요 특성은 다음과 같다. 1. 청일뽕 자연교잡 실생에 콜히친 처리하여 얻어진 4개체 411호와 국상 21호를 교배하여 얻어진 3배체로 백상형 (Morus alba L.)에 속하는 암나무이다. 2. 수형은 직립성이고 개량뽕에 비하여 가지수는 큰 차이가 없으며, 평균가지 길이와 마디사이길이는 짧고 가지 굵기는 굵다. 3. 발아기는 중뽕이며, 잎의 크기는 대형으로 잎두께는 약간 두꺼우며 타원형과 3-5열엽이 섞여 존재하지만 5열엽이 많다. 3. 뽕 수량은 개량뽕보다 20% 많으며, 사료가치는 청운뽕과 같은 수준으로 비교적 우량하다. 4. 뽕나무 오갈병 저항성은 개량뽕보다 약간 약하나, 눈마름병, 줄기마름병 및 축엽세균병은 개량뽕과 비슷한 수준이다. 5. 춘추잠기 겸용 큰누에용 뽕으로 동해상습지를 제외한 전국에 재배할 수 있다.

• Journal of Plant Biotechnology
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• 제36권4호
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• pp.320-326
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• 2009

The carbon dioxide concentration of the atmosphere is projected to increase by almost 50% over the first 50 years of this century. The major cause of this increase is continued combustion of fossil fuels. As a result, the significant changes in climate that have already occurred will be amplified, in particular a global temperature increase. Renewable energy production has a central role to play in abating net $CO_2$ emissions to a level that will arrest the development of global warming. Especially, biomass crops are becoming increasingly important as concerns grow about climate change and the need to replace carbon dioxideproducing fossil fuels with carbon-neutral renewable sources of energy. To succeed in this role, biomass crop has to grow rapidly and yield a reliable, regular harvest. A prime candidate is Miscanthus, or Asian elephant grass, a perennial species that produces over 3 metres of bamboo-like stems in a year. Miscanthus species are typically diploid or tetraploid. Hybrids between species with different ploidy levels result in the highly productive triploid hybrids, M. ${\times}$ giganteus. Here we will detail the Miscanthus characteristics desired of a biomass fuel crop.

• 한국초지조사료학회지
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• 제17권1호
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• pp.1-10
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• 1997

Unreduced (2n) gametes are meiotic products (pollen or egg) having a sporophytic (somatic) chromosome number, resulting from abnormalities during either microsporogenesis or megasporogenesis. They occur naturally at a low frequency in many plant species. Unreduced (2n) gametes in plants can be identified for four possible ways as follow i) pollen size and/or shape differences between haploid (n) and diploid (2n) pollen, ii) ploidy analysis (chromosome number) of progeny or meiotic analysis (presence of dyads andlor triads at the microspore stage), iii) progeny performance and fertility and iv) dosage of isozyme and DNA markers. Unreduced (2n) gametes can be an effective breeding tool in synthesizing new cultivars, providing a unique method to maximizing heterozygosity, i.e., transferring a large proportion of the non-additive genetic effects (intra- and inter- locus interactions) h m parent to offspring and can also be used to overcome infertility of interploidy crosses. Sexual polyploidization through 2n gametes has been a major route to the formation of naturally occurring polyploids. The three mechanisms of 2n pollen formation in potato have been discovered as follow: i) parallel spindles (ps) or tripolar spindles (ts), ii) premature cytokinesis (pc-I, pc-2) and iii) synaptic mutants (sy-2, sy-3, sy-4). Genetic analysis indicated that the mechanisms of 2n gamete formation were controlled by single recessive gene in potato, alfalfa, red clover, etc., and by two recessive genes in wheat. The use of 2n gametes which can efficiently transfer germplasm fiom wild relatives to cultivated species, especially fiom diploid to tetraploid could make a contribution to the improvement of germplasm base in breeding programs.

• 한국초지조사료학회지
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• 제20권1호
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• pp.1-6
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• 2000

Improvement of cold-tolerance of Italian ryegrass(Lo1ium multiflorum L.) is an important breeding objectivefor enlargement of cultivation area in Korea. To develop the cold-tolerant variety of Italian ryegrass,cold-tolerant clones survived under - 13- - 14$^{\circ}$C of minimum average air temperature in January wereselected in the area of Dun Nae, Kwangwon Province. The 5 lines of Italian ryegrass clones selected werepolycrossed for seed production. Synthetic seeds were examined on growth characters and forage productionsin Suwon from 1996 to 1998, in Unbong 1997 and in Younchun 1998, respectively. The growth charactersand forage productions of Italian ryegrass variety named as "Hwasan 101" were summarized as follow ;ltalian ryegrass variety, Hwasan 101 was tetraploid and showed semi-prostrate growth habit in autumn andwas not only dark green in leaf color and broad in flag leaf width but also excellent in leafiness. Also, itshowed low plant height at first heading date of 20th May and excellent lodging tolerance. Expecially,Hwasan 101 in all trial regions was 80 -90% of winter survivals that was higher than 60 -90% of Florida80 and Marshall varieties. Therefore we estimate that Hwasan 101 can be cultivated in regions that is higherthan -9$^{\circ}$C in minimum average air temperature and lower than 400m in sea level. In forage production ofHwasan 101, fresh weight and total digestible nutrient(TDN) yield were 66,940 and 6,551kghq and drymatter yield was 10,050kgha. In conclusion, Italian ryegrass, Hwasan 101 was medium and late maturingvariety but excellent in cold-tolerance, lodging-tolerance, leafiness, and forage production.(Key words : Italian ryegrass, Cold tolerance, New variety)ian ryegrass, Cold tolerance, New variety)