• Toxicological Research
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• v.33 no.2
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• pp.107-118
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• 2017

Although alternative test methods based on the 3Rs (Replacement, Reduction, Refinement) are being developed to replace animal testing in reproductive and developmental toxicology, they are still in an early stage. Consequently, we aimed to develop alternative test methods in male animals using mouse spermatogonial stem cells (mSSCs). Here, we modified the OECD TG 489 and optimized the in vitro comet assay in our previous study. This study aimed to verify the validity of in vitro tests involving mSSCs by comparing their results with those of in vivo tests using C57BL/6 mice by gavage. We selected hydroxyurea (HU), which is known to chemically induce male reproductive toxicity. The 50% inhibitory concentration ($IC_{50}$) value of HU was 0.9 mM, as determined by the MTT assay. In the in vitro comet assay, % tail DNA and Olive tail moment (OTM) after HU administration increased significantly, compared to the control. Annexin V, PI staining and TUNEL assays showed that HU caused apoptosis in mSSCs. In order to compare in vitro tests with in vivo tests, the same substances were administered to male C57BL/6 mice. Reproductive toxicity was observed at 25, 50, 100, and 200 mg/kg/day as measured by clinical measures of reduction in sperm motility and testicular weight. The comet assay, DCFH-DA assay, H&E staining, and TUNEL assay were also performed. The results of the test with C57BL/6 mice were similar to those with mSSCs for HU treatment. Finally, linear regression analysis showed a strong positive correlation between results of in vitro tests and those of in vivo. In conclusion, the present study is the first to demonstrate the effect of HU-induced DNA damage, ROS formation, and apoptosis in mSSCs. Further, the results of the current study suggest that mSSCs could be a useful model to predict male reproductive toxicity.

• Journal of Advanced Navigation Technology
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• v.19 no.2
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• pp.91-97
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• 2015

This paper specifies several considerations about assessing safety-critical software in the aerospace domain. In order to evaluate safety critical software in the aerospace industry, it is required to identify an information of evaluation criteria of software under evaluation. The information is specified in the standard, but determination of evaluation criteria cannot be decided by itself and depends on the results of safety assessment of a system and system design. Thus, this paper explains required information of system development standard and safety assessment standard to determine software evaluation criteria. It surveys existing methodologies about evaluating software, and suggests method which is adapted to evaluation of an advanced surface movement guidance and control system (A-SMGCS) software.

• Annual Conference of KIPS
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• 2008.11a
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• pp.496-499
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• 2008

공공부분 S/W사업은 분리발주가 원칙이고 일괄발주가 예외지만 현재 많은 공공부분 S/W사업은 일괄 발주 사업으로 진행되고 있다. 일괄발주는 SI업체가 S/W업체도 선택하여 입찰 및 개발하기 때문에 발주자가 원하는 품질 좋은 S/W를 선택할 수 없고, 발주자가 S/W를 업그레이드 및 확장할 때 SI업체에 의존하게 되는 단점이 있다. 이런 단점을 보완하고, S/W분리발주를 활성화시키기 위해 지식경제부(구 정보통신부)에서는 S/W분리발주 매뉴얼을 작성하여 보급 및 교육을 하고 있으며, 발주처에서는 품질이 우수한 S/W를 선택하기 위한 S/W분리발주 BMT를 공인된 시험기관인 한국정보통신기술협회(TTA) SW 시험인증센터에서 BMT를 실시하고 있다. 본 논문은 위협관리시스템(TMS) S/W분리발주 BMT를 공인된 국가 시험인증기관인 한국정보통신기술협회(TTA) SW시험인증센터에서 수행한 사례를 기술하였다

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.22 no.10
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• pp.968-973
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• 2012

Slam testing is a mandatory testing process to evaluate the fatigue resistance of a door plate module before delivering it to car makers. This process is very hard job to complete it since the testing facilities are considerably expensive and the required testing time is relatively very long, i.e. more than eight days for a single specimen. In this paper, an accelerated testing method of a door plate module is proposed using vibration test equipment instead of the current one by exposing to the critical excitation of a door glass. Under the proposed excitation method, the similar testing result can be evaluated within less than two hours. The suitability of the proposed testing method was demonstrated by comparing failure modes of both the current testing method and the proposed one.

• Journal of the Ergonomics Society of Korea
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• v.30 no.2
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• pp.331-338
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• 2011

Objective: The present study developed a comprehensive usability testing and analysis framework based on a physical interface model of product and user and applied the proposed framework to usability testing of canister-type vacuum cleaner. Background: The development of a user-centered product design is important to satisfy customers who want to use the product with ease of use and to keep the manufacturer competitive in the market. Method: The proposed testing and analysis framework consists of (1) characterization of physical product-user interface, (2) preparation and administration of usability testing questionnaire, and (3) analysis and interpretation of usability testing results. A usability evaluation of five vacuum cleaners was planned and administered based on the proposed framework and its analysis produced detailed and overall usability testing results for various aspects such as tasks, usability criteria, and design components. Results: The testing results were further utilized to identify usability problems and preferred design features of the vacuum cleaners. Conclusion: The proposed usability testing and analysis framework was found effective to identify preferred features and problems of a product design in a systematic, holistic manner. Application: The proposed framework can be of effective use for practitioners of product design and development to obtain comprehensive, quantitative usability testing information in a systematic manner.

• Journal of the Korean Society of Propulsion Engineers
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• v.2 no.2
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• pp.108-117
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• 1998

Testing and evaluation was carried out to certify quality assurance of localized engine parts using accelerated mission test method, which has been used to verify qualification or substantiation of the developed engine, improved engine components and engineering changed parts since 1976. Because AMT is not a familiarized testing for quality assurance of engine and localized engine parts in korea, through this testing and evaluation, it is presented the concepts, test procedures and evaluation method of AMT and is proposed the possibility of execution AMT, as a tool for qualification or substantiation test of a development engine or components.

• Toxicological Research
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• v.23 no.2
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• pp.173-177
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• 2007

The GLP contains specific language concerning characterization of the test, control and reference substances used in toxicity studies. This paper will describe and discuss what types of documents are required to support test/reference substance characterization under GLP system. The purpose of this article is to present an overview of data needed in the characterization package that will adequately define the substance. Most sponsors use a certificate of analysis (COA) to communicate the test sub-stance characterization status information to the contracting research organizations. The COA should provide the test $material^{\circ}{\phi}s$ characterization results, substance storage requirements, expiration dates, verification of the collection of the retention sample, archival location of the data to support the characterization and GLP compliance status of the characterization.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2002.05a
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• pp.191-194
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• 2002

The tensile properties of materials can be obtained just in accordance with conventional tensile testing methods which are described in several standards. However, the standard testing methods cannot be applicable due to the destructive testing procedure and specimen size requirement for some cases including on-service facility materials. Therefore, simple, non-destructive and advanced indentation technique was proposed. This test measures indentation load-depth curve during indentation and analyzes the mechanical properties related to deformation and fracture. In this paper, the research trend of non-destructive evaluation of tensile properties using advanced indentation system and its application fields are reviewed and discussed.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.32 no.1
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• pp.94-100
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• 2000

This study was carried out to understand the present status of the quality evaluation capability of domestic fine paper industry. Six light weight coated papers and one wood containing printing and writing paper with similar basis weights were produced by seven paper companies and their properties were evaluated at four different testing laboratories of domestic fine paper mills. Paper properties including basis weight , thickness , tensile, tear , brightness , opacity, etc were examined , and the repeat ability and reproducibility of the test results were analyzed. Results showed that repeatability of the measurements within the laboratory was satisfactory . Reproducibility of the measurements between laboratories, however, was not satisfactory. This showed that strict implementation of the standard testing methods and periodic calibration of testing equipments should be made to improve quality evaluation capability of domestic fine paper industry.

• Journal of the Korean Society for Nondestructive Testing
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• v.22 no.1
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• pp.32-37
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• 2002

The presence of hydrogen in industrial plants is a source of damage. Hydrogen attack is one such form of degradation and often causing large tube ruptures that necessitate an immediate shutdown. Hydrogen attack may reduce the fracture toughness as well as the strength of steels. This reduction is caused partially by the presence of cavities and microcracks at the grain boundaries. In the past several techniques have been used with limited results. This paper describes the application of an ultrasonic velocity and attenuation in hydrogen damage. Ultrasonic tests showed a decrease in wave velocity and an increase in attenuation. Such results demonstrate the potential for ultrasonic nondestructive testing to quantify damage. Based on this study, reliable recommendation is suggested to detect hydrogen attack.