Perchlorate ($ClO_4^-$) is a contaminant found in surface water and soil/ground water. Autotrophic perchlorate-reducing bacteria (PRB) use hydrogen gas ($H_2$) as an electron donor to remove perchlorate. Since iron corrosion can produce $H_2$, feasibility of autotrophic perchlorate-removal using zero-valent iron (ZVI) was examined in this study using activated sludge that is easily available from a wastewater treatment plant. Batch test showed that activated sludge microorganisms could successfully degrade perchlorate in the presence of ZVI. The perchlorate biodegradation was confirmed by molar yield of $Cl^-$ as perchlorate was degraded. Scanning electron microscope revealed that rod-shaped microorganisms on the surface of iron particles used for the autotrophic perchlorate-removal, suggesting that iron particles could serve as supporting media for the formation of biofilm as well. DGGE analyses revealed that microbial profile of the inoculum (activated sludge) was different from that of biofilm sample obtained from the ZVI-added enrichment culture used for $ClO_4^-$-degradation. A major band of the biofilm sample was most closely related to the class Clostridia.
The CM247LC, a Ni-based superalloy material used for gas turbine hot gas path parts, is casted using directionally solidified technology to analyze the mechanical properties and microstructures through HIP (Hot Isostatic Pressing) and post-heat treatment, and to derive optimal HIP treatment conditions. The CM247LC material is being researched in various ways as an alternative material for prototyping gas turbine blades. In particular, the blade rotating part is exposed and operated in a high temperature and high-pressure environment, and when damaged, it may cause huge economic losses. Therefore, in order to use the CM247LC material as prototyping materials for gas turbine blades, the reliability of the microstructure and mechanical properties must be verified. In this study, after casting rod test specimens using CM247LC material by directionally solidified technology, after that the specimens were performed by HIP treatment and post-heat treatment to test two HIP conditions designed by KEPCO to derive the possibility of prototyping of CM247LC material and optimization of HIP treatment conditions. Additionally, the properties of CM247LC material were compared to the GTD111DS material using for 1,300℃ class gas turbine blades.
This study was conducted to investigate the occurrence and characterization of tobacco mosaic virus(TMV) in Chinese foxglove isolated from the field of the Chonbuk province(Jinan, Jangsu, Jeongeup). TMV was detected in all three regions and confirmed positive reaction by ELISA test. In the host range test, Chenopodium amaranticola, Nicotiana glutinosa, N. tabacum cv. 'Bright yellow', N. tabacum cv. 'KY57, Datura stramonium were locally infected with the virus. The virus produced mosaic symptom on inoculated leaves of N. tabacum cv. 'Samson'. However, Chenopodium quinoa, Glycine max, Raphanus sativus, Cucumis sativus, Cucurbita moschata, Brassica rape and Lycopersion esculentum did not show any symptoms. TMV particles were revealed as a stiff rod shape by transmission electron microscopic(TEM) and measured as 300 nm in length with 18 nm in diameter. Total RNA was extracted from showing symptom loaves infected with TMV and the reverse transcriptionpolymerase chain reaction (RTPCR) obtained 531 bp DNA product of RNA with specific primer used. The capsid protein of TMVRE showed higher amino acid sequence homology(97.7%) with TMVTo than with TMVP(72.2%). The capsid protein of TMV152 showed same amino acid sequence homology with TMVF. The result of comparison of nucleotides sequence homology between TMVRE strain and other TMV strain showed 94% homology with others except TMVP(67.3%) and TMV C(68.6%).
For the interests in the economical and safe design of foundation system, the concern on the piled raft or disconnected piled raft foundation system is increasing now. In this study, the behavior and the effects of the disconnected piled raft foundation not studied actively in this country were examined using the triaxial compression tests in place of laboratory model tests. The triaxial test samples were prepared with Jumunjin standard sand and the carbon rods, which simulate the ground soil and piles respectively. After the sample in which carbon rods were arranged was laid inside the triaxial chamber, the confining pressure was applied and then loading test was conducted. To analyze the reinforcing effects of the disconnected piled raft foundation, a few number of tests were carried out by changing the number, the diameter and the length of the model piles. As a result of this study, in the disconnected piled raft foundation system, even though the number of pile is few and the diameter of pile is small, the settlement of the foundation system decreased greatly.
This study examined structural analysis of supports in tunnel and displacement and underground stress of tunnel by measurement, in order to evaluate the performance of high-strength lattice girders developed as a substitute for H-profiles. According to the three-dimensional nonlinear structural analysis results of the tunnel support, the load and displacement relationship between the H-profiles and the high-strength lattice girders showed almost the same behavior, and the maximum load of the high-strength lattice girders were 1.0 to 1.2 times greater than the H-profiles. By the results of the three-dimensional tunnel cross-section analysis of the supports, the axial force was occurred largely in the lower left and right sides of the tunnel, and showed a similar trend to the field test values. In the results of the measurement of the roof settlement and rod extension, the final displacement of the steel arch rib (H-profile) and high-strength lattice girder section in tunnel was converged to a constant value without significant difference within the first management standard of 23.5 mm. According to the results of underground displacement measurement, the final change amount of the two support sections showed a slight displacement change, but converged to a constant value within the first management standard of 10 mm. By the results of measurement of shotcrete stress and steel arch rib stress, the final change amount of the two support sections showed a slight stress change, but converged to a constant value within the first management standard of 81.1 kg/㎠ and 54.2 tonf.
Purpose: This study is to investigate if the improvement of visual sensory (VS) by amblyopia treatment affects the ocular functions in refractive errors, accommodative errors and phoria at distance and near. Methods: 10 subjects (17 eyes, mean age of $10.7{\pm}2.9$ years) who treated amblyopia completely, were participated for this study. Refractive errors, accommodative errors, and distance and near phoria were compared between before and after treatments of amblyopia. Refractive errors and accommodative errors at 40 cm were measured using openfield auto-refractor (NVision-5001, Shin Nippon, Japan) and using monocular estimated method (MEM) respectively. Phoria was determined at 3 m for distance and at 40 cm for near using Howell phoria card, cover test or Maddox rod. Results: Mean corrected visual acuity (CVA) significantly increased from $0.46{\pm}0.11$ (decimal notation) for before amblyopia treatment to a level of $1.03{\pm}0.13$ for after amblyopia treatment (p < 0.001). For spherical refractive error, hyperopia significantly decreased from $+2.29{\pm}0.86D$ to a level of $+1.1{\pm}2.38D$ (p < 0.05) but astigmatism did not significantly change; $-1.80{\pm}1.41D$ for before treatment and $-1.65{\pm}1.30D$D for after treatment (p > 0.05). Accommodative error significantly decreased from accommodative lag of $+1.1{\pm}0.75D$ to a level of $+0.5{\pm}0.59D$ (accommodative lag) (p < 0.05). Distance phoria significantly changed from eso $2.9{\pm}6.17PD$ (prism diopters) to a level of eso $0.2{\pm}3.49PD$ (p < 0.05), and near phoria also significantly changed from eso $0.4{\pm}2.32PD$ to level of exo $2{\pm}4.9PD$ (p < 0.05). There was a high correlation (r = 0.88, p < 0.001) between improvement of visual acuity and decrease of accommodative lag. Conclusions: Hyperopic refractive error decreased with improvement of CVA or VS by amblyopia treatment. And the improvement of VS by amblyopia treatment also improved accommodative error, and changed phoria coupled with accommodation.
The biocompatibility of the titanium has been estabilished through various experimental studies such as cell culture toxicity test, pyrogen test, mutagen test and others. In order to confirm biocompatibility after fabrication of titanium and to clarify the difference between the bone reaction after insertion of the lathed titanium rods and the bone reaction after insertion of the finished and polished rods, both rods were implanted into the proximal femur of a rabbit. Histologic reactions in the bone were observed according to the ASTM standards at the intervals of 6 weeks, 12 weeks and 26 weeks after implantation. The result were as follows : In 6 weeks after implantation of lathed titanium rods, inflammatory reactions, such as minimal degree infiltration of polymorphonuclear leukocytes and lymphocytes were observed in all cases. This was thought to he caused by surgical trauma. However, inflammatory cell infiltration was not seen after implantation of polished and finished rods in all cases. The cellular infiltration and the histologic reaction of the hone after implantation of lathed group were significantly more pronounced than those after implantation of the finished group. In 12 weeks after implantation of lathed rods, two of four cases revealed a minimal degree of cellular infiltration. No inflammatory cell infiltration was demonstrated after implantation of the finished group. The cellular infiltration and histologic reaction seemed to be more pronounced in the lathed group, but they were not significant statistically. At 26 weeks after implantation of the lathed and finished group, there was no cellular infiltration in both groups. New bone formation was observed up 26 weeks, and no difference between lathed titanium rods and finished titanium rods were apparent. Mild bone necrosis was observed in 1 case out of 11 cases in which lathed titanium rods were implanted. Bone necrosis was not observed in the finished titanium rod group. Fibrosis was observed in both groups, but differences were not significant between the experimental groups. In the lathed titanium rods group and the shorter interval group, inflammatory cell infiltration was significantly higher. Finished titanium rods and longer interval groups had markedly decreased tendences in histologic reaction ratings. As a conclusion, although certificated titanium might be safe to use, difference of biocompatibility were observed depending on the method of surface finish. By identifying biocompatibility as a long-term standardized animal study, we can develop progressed internal fixation device that is safe for human beings.
Perchlorate ($ClO_4^-$) is a contaminant found in surface water and soil/ground water. Microbial removal of perchlorate is the method of choice since microorganisms can reduce perchlorate into harmless end-products. Such microorganisms require an electron donor to reduce perchlorate. Conventional perchlorate-removal techniques employ heterotrophic perchlorate-reducing bacteria that use organic compounds as electron donors to reduce perchlorate. Since continuous removal of perchlorate requires a continuous supply of organic compounds, heterotrophic perchlorate removal is an expensive process. Feasibility of autotrophic perchlorate-removal using elemental sulfur granules and activated sludge was examined in this study. Granular sulfur is relatively inexpensive and activated sludge is easily available from wastewater treatment plants. Batch tests showed that activated sludge microorganisms could successfully degrade perchlorate in the presence of granular sulfur as an electron donor. Perchlorate biodegradation was confirmed by molar yield of $Cl^-$ as the perchlorate was degraded. Scanning electron microscope revealed that rod-shaped microorganisms on the surface of sulfur particles were used for the autotrophic perchlorate-removal, suggesting that sulfur particles could serve as supporting media for the formation of biofilm as well. DGGE analyses revealed that microbial profile of the inoculum (activated sludge) was different from that of the biofilm sample obtained from enrichment culture that used sulfur particles for $ClO_4^-$-degradation.
Objective: The purposes of this study were to evaluate the reproducibility and reliability of head posture obtained by registering outer canthus as a soft tissue landmark with the Outer Canthus Indicator (OCI). Methods: Twenty-one adults with normal facial morphology were enrolled in this study (mean age $27.5\;{\pm}\;1.72$ years). To register initial head posture, height of the outer canthus from the ear rod plane was measured using OCI. Head posture was reproduced by moving the head upwards and downwards until the outer canthus was in a straight line with the indicator set at a registered height. After the head posture is reproduced by two operators after two days, lateral photographs were taken. Computerized photometric analyses of the photographs were performed. Results: The head rotations around the transverse axis were $0.69\;{\pm}\;0.43^{\circ}$, $0.98\;{\pm}\;0.65^{\circ}$ from each of the two operators. Standard errors were $0.09^{\circ}$ and $0.14^{\circ}$ each, which were similar to results from past research findings. There were no significant differences between the data from the two operators (p > 0.05). There were no correlations between the head rotation around the horizontal and vertical axes (p > 0.05). Conclusions: The present study suggests that OCI-registered head posture may minimize errors from vertical head rotation in cephalometry and photometry.
Journal of the Korean Recycled Construction Resources Institute
/
v.6
no.1
/
pp.59-65
/
2018
RC(Reinforced Concrete) structures are exposed to various exterior conditions, and the performances of both chloride resistance and freezing/thawing action are evaluated for those exposed to corrosive environment-sea shore. Recently developed FRP Hybrid Bars which is coated with glass fiber and epoxy with core steel has an engineering advantage of higher Elasticity than FRP rod. In this work, corrosion resistance, weight loss, and bond strength are evaluated for the FRP Hybrid Bar tested through freezing/thawing action for 300cycles. The double coated FRP Hybrid Bar shows the least weight loss without defection due to freezing/thawing action. Bond strength in FRP Hybrid Bar increases to 120% of normal steel through torturity effect with Si-coating. Bond strength in normal steel shows 0.86~0.89times in 3-day corrosion acceleration and 0.35~0.38times in 5-day corrosion acceleration, however, that in FRP Hybrid Bar shows little changes in bond strength before and after freezing/thawing action.
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