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http://dx.doi.org/10.5352/JLS.2011.21.10.1473

Autotrophic Perchlorate-Removal Using Elemental Sulfur Granules and Activated Sludge: Batch Test  

Han, Kyoung-Rim (Department of Environmental Engineering, Dong-A University)
Kang, Tae-Ho (Korea Science Academy of KAIST)
Kang, Hyung-Chang (Korea Science Academy of KAIST)
Kim, Kyung-Hun (Korea Science Academy of KAIST)
Seo, Deuk-Hwa (Korea Science Academy of KAIST)
Ahn, Yeong-Hee (Department of Environmental Engineering, Dong-A University)
Publication Information
Journal of Life Science / v.21, no.10, 2011 , pp. 1473-1480 More about this Journal
Abstract
Perchlorate ($ClO_4^-$) is a contaminant found in surface water and soil/ground water. Microbial removal of perchlorate is the method of choice since microorganisms can reduce perchlorate into harmless end-products. Such microorganisms require an electron donor to reduce perchlorate. Conventional perchlorate-removal techniques employ heterotrophic perchlorate-reducing bacteria that use organic compounds as electron donors to reduce perchlorate. Since continuous removal of perchlorate requires a continuous supply of organic compounds, heterotrophic perchlorate removal is an expensive process. Feasibility of autotrophic perchlorate-removal using elemental sulfur granules and activated sludge was examined in this study. Granular sulfur is relatively inexpensive and activated sludge is easily available from wastewater treatment plants. Batch tests showed that activated sludge microorganisms could successfully degrade perchlorate in the presence of granular sulfur as an electron donor. Perchlorate biodegradation was confirmed by molar yield of $Cl^-$ as the perchlorate was degraded. Scanning electron microscope revealed that rod-shaped microorganisms on the surface of sulfur particles were used for the autotrophic perchlorate-removal, suggesting that sulfur particles could serve as supporting media for the formation of biofilm as well. DGGE analyses revealed that microbial profile of the inoculum (activated sludge) was different from that of the biofilm sample obtained from enrichment culture that used sulfur particles for $ClO_4^-$-degradation.
Keywords
Autotroph; biodegradation; electron donor; elemental sulfur granules; perchlorate; reduction;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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