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Development of Near Infrared Spectroscopy(NIRS) Equation of Crude Protein in Wheat Germplasm

  • Hyemyeong Yoon;Myung-Chul Lee;Yumi Choi;Myong-Jae Shin;Sejong Oh
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.100-100
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    • 2020
  • Wheat is mainly composed of carbohydrate but it contains a moderate amount of protein, which gives a very useful characteristics to flour food such as the unique elasticity and stickiness of the dough. We developed a calibration equation for analyzing crude protein content using Near Infrared Spectroscopy to quick analyze the crude protein content of wheat germplasm stored in the National Agrobiodiversity Center, RDA, Korea. The 1,798 wheat germplasms were used to draw up the calibration formula. The crude protein's interval distribution of 1,798 wheat germplasms used for the calibration was 7.04-20.84%, the average content was 13.2%, and standard deviation was 2.6%. The germplasms distribution was composed of a suitable group for the preparation of the calibration formula because the content distribution was a normal, excluding the 13.0-15.5% content section. In order to verify the applicability of the NIRS prediction model, we measured the crude protein content of the 300 wheat germplasms that were not used for the calibration using both Kjeldahl analysis and NIR spectrum. The analysis value calculated using each method were statistically processed, and the test results and statistical indicators of the predictive model were compared. As a result, The R2 value of the optimized NIRS prediction model was 0.997, and the Standard error of Calibration value(SEC) was 0.132, and slope value was 1.000. With prediction model selection, compared to Kjeldahl method, R2 values were 0.994(Kjeldahl), 0.998(NIRS), and the SEC value were 0.191 and 0.132, respectively, comparing the statistical indices of the forecast model. And slope value were 1.013, 1.000, respectively. The analysis of crude protein content by the NIRS predictive model developed by each statistical index showing similar figures is judged to show a high degree of correlation with the Kjeldahl analysis. The proven calibration equation will be used to measure the crude protein content of wheat germplasms held by the National Agrobiodiversity Center, and by dividing the wheat germplasms by their use according to the crude protein content, it will provide useful information to relevant researchers.

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Research on the Development of Microneedle Arrays Based on Micromachining Technology and the Applicability of Parylene-C (미세가공 기술 기반의 마이크로니들 어레이 개발 및 패럴린 적용 가능성에 관한 연구)

  • Dong-Guk Kim;Deok-kyu Yoon;Yongchan Lee;Min-Uk Kim;Jihyoung Roh;Yohan Seo;Kwan-Su Kang;Young Hun Jeong;Kyung-Ah Kim;Tae-Ha Song
    • Journal of Biomedical Engineering Research
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    • v.44 no.6
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    • pp.404-413
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    • 2023
  • In this research, we studied the development of a SUS304 microneedle array based on microfabrication technology and the applicability of Parylene-C thin film, a medical polymer material. First of all, four materials commonly used in the field of medical engineering (SUS304, Ti, PMMA, and PEEK) were selected and a 5 ㎛ Parylene-C thin film was deposited. The applicability of Parylene-C coating to each material was confirmed through SEM analysis, contact angle measurement, surface roughness(Ra) measurement, and adhesion test according to ASTM standards for each specimen. Parylene-C thin film was deposited based on chemical vapor deposition (CVD), and a 5 ㎛ Parylene-C deposition process was established through trial and error. Through characteristic experiments to confirm the applicability of Parylene-C, SUS304 material, which is the easiest to apply Parylene-C coating without pretreatment was selected to develop a microneedle array based on CNC micromachining technology. The CNC micromachining process was divided into a total of 5 steps, and a microneedle array consisting of 19 needles with an inner diameter of 200 ㎛, an outer diameter of 400 ㎛, and a height of 1.4 mm was designed and manufactured. Finally, a 5 ㎛ Parylene-C coated microneedle array was developed, which presented future research directions in the field of microneedle-based drug delivery systems.

Prediction of Microstructure and Hardness of the Ductile Cast Iron Heat-treated at the Intercritical Temperatures (임계간 온도에서 열처리한 구상흑연주철의 미세조직 및 경도 예측)

  • Nam-Hyuk Seo;Jun-Hyub Jeon;Soo-Yeong Song;Jong-Soo Kim;Min-Su Kim
    • Journal of Korea Foundry Society
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    • v.43 no.6
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    • pp.279-285
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    • 2023
  • In order to predict the mechanical properties of ductile cast iron heat treated in an intercritical temperature range, samples machined from cast iron with a tensile strength of 450 MPa were heat-treated at various intercritical temperatures and air-cooled, after which a microstructural analysis and Brinell hardness test were conducted. As the heat treatment temperature was increased in the intercritical temperature range, the ferrite fraction in the ductile cast iron decreased and the pearlite fraction increased, whereas the nodularity and nodule count did not change considerably from the corresponding values in the as-cast condition. The Brinell hardness values of the heat-treated ductile cast iron increased gradually as the heat treatment temperature was increased. Based on the measured alloy composition, the fraction of each stable phase and the hardness model from the literature, the hardness of the ductile cast iron heat treated in the intercritical temperature range was calculated, showing values very similar to the measured hardness data. In order to check whether it is possible to predict the hardness of heat-treated ductile cast iron by using the phase fraction obtained from thermodynamic calculations, the volumes of graphite, ferrite, and austenite in the alloy were calculated for each temperature condition. Those volume fractions were then converted into areas of each phase for hardness prediction of the heat-treated ductile cast iron. The hardness values of the cast iron samples based on thermodynamic calculations and on the hardness prediction model were similar within an error range up to 27 compared to the measured hardness data.

The Effects of Dynamic Visual by Students' Field Independence-Dependence on Learning with Multiple Representations: Focused on Connecting Errors and Conceptual Understanding (다중표상학습에서 학생들의 장독립성.장의존성에 따른 동화상의 효과: 연계 오류와 개념 이해를 중심으로)

  • Noh, Tae-Hee;Moon, Se-Jeong;Lee, Jong-Hyun;Seo, Hyun-Ju;Kang, Hun-Sik
    • Journal of The Korean Association For Science Education
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    • v.29 no.2
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    • pp.156-167
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    • 2009
  • This study investigated the effects of dynamic visual on students' field independence-dependence on connecting errors and conceptual understanding in learning chemistry concepts with multiple representations. Seventh graders (N=123) at a co-ed middle school were assigned to a static visual (SV) group learning with text and static visual, and a dynamic visual (DV) group learning with text and dynamic visual. The students then learned 'Boyle's Law' and 'Charles's Law' for two class periods. Results revealed that the percentages of the DV group were lower than those of the SV group on connecting errors. However, the percentages of the students' connecting errors were still high regardless of their field independence-dependence. There was a little different tendency in the percentages of connecting errors between the two groups by students' field independence-dependence according to the types of connecting errors. The scores of the DV group were significantly higher than those of the SV group in a test on conceptual understanding. However, there was no significant interaction between the instruction and the students' field independence-dependence. Educational implications of these findings are discussed.

The Measurement Algorithm for Microphone's Frequency Character Response Using OATSP (OATSP를 이용한 마이크로폰의 주파수 특성 응답 측정 알고리즘)

  • Park, Byoung-Uk;Kim, Hack-Yoon
    • The Journal of the Acoustical Society of Korea
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    • v.26 no.2
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    • pp.61-68
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    • 2007
  • The frequency response of a microphone, which indicates the frequency range that a microphone can output within the approved level, is one of the most significant standards used to measure the characteristics of a microphone. At present, conventional methods of measuring the frequency response are complicated and involve the use of expensive equipment. To complement the disadvantages, this paper suggests a new algorithm that can measure the frequency response of a microphone in a simple manner. The algorithm suggested in this paper generates the Optimized Aoshima's Time Stretched Pulse(OATSP) signal from a computer via a standard speaker and measures the impulse response of a microphone by convolution the inverse OATSP signal and the received by the microphone to be measured. Then, the frequency response of the microphone to be measured is calculated using the signals. The performance test for the algorithm suggested in the study was conducted through a comparative analysis of the frequency response data and the measures of frequency response of the microphone measured by the algorithm. It proved that the algorithm is suitable for measuring the frequency response of a microphone, and that despite a few errors they are all within the error tolerance.

A Study on LNG Quality Analysis using a Raman Analyzer (라만분석기를 이용한 LNG 품질 분석 실증 연구)

  • Kang-Jin Lee;Woo-Sung Ju;Yoo-Jin Go;Yong-Gi Mo;Seung-Ho Lee;Yoeung-Chul Kim
    • Korean Chemical Engineering Research
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    • v.62 no.1
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    • pp.70-79
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    • 2024
  • Raman analyzer is an analytical technique that utilizes the "Raman effect", which occurs when light is scattered by the inherent vibrations of molecules. It is used for molecular identification and composition analysis. In the natural gas industry, it is widely used in bunkering and tank lorry fields in addition to LNG export and import terminals. In this study, a LNG-specific Raman analyzer was installed and operated under actual field conditions to analyze the composition and principal properties (calorific value, reference density, etc.) of LNG. The measured LNG composition and calorific value were compared with those obtained by conventional gas chromatograph that are currently in operation and validated. The test results showed that the Raman analyzer provided rapid and stable measurements of LNG composition and calorific value. When comparing the calorific value, which serves as the basis for LNG transactions, with the results from conventional gas chromatograph, the Raman analyzer met the acceptable error criteria. Furthermore, the measurement results obtained in this study satisfied the accuracy criteria of relevant international standards (ASTM D7940-14) and demonstrated similar outcomes compared to large-scale international demonstration cases.

Vision-based Method for Estimating Cable Tension Using the Stay Cable Shape (사장재 케이블 형태를 이용하여 케이블 장력을 추정하는 영상기반 방법)

  • Jin-Soo Kim;Jae-Bong Park;Deok-Keun Lee;Dong-Uk Park;Sung-Wan Kim
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.28 no.1
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    • pp.98-106
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    • 2024
  • Due to advancements in construction technology and analytical tools, an increasing number of cable-stayed bridges have been designed and constructed in recent years. A cable is a structural element that primarily transmits the main load of a cable-stayed bridge and plays the most crucial role in reflecting the overall condition of the entire bridge system. In this study, a vision-based method was applied to estimate the tension of the stay cables located at a long distance. To measure the response of a cable using a vision-based method, it is necessary to install feature points or targets on the cable. However, depending on the location of the point to be measured, there may be no feature points in the cable, and there may also be limitations in installing the target on the cable. Hence, it is necessary to find a way to measure cable response that overcomes the limitations of existing vision-based methods. This study proposes a method for measuring cable responses by utilizing the characteristics of cable shape. The proposed method involved extracting the cable shape from the acquired image and determining the center of the extracted cable shape to measure the cable response. The extracted natural frequencies of the vibration mode were obtained using the measured responses, and the tension was estimated by applying them to the vibration method. To verify the reliability of the vision-based method, cable images were obtained from the Hwatae Bridge in service under ambient vibration conditions. The reliability of the method proposed in this study was confirmed by applying it to the vibration method using a vision-based approach, resulting in estimated tensions with an error of less than 1% compared to tensions estimated using an accelerometer.

Manufacture and Qualification of Composite Main Reflector of High Stable Deployable Antenna for Satellite (위성용 전개형 고안정 반사판 안테나 주반사판 제작 및 검증)

  • Dong-Geon Kim;Hyun-Guk Kim;Dong-Yeon Kim;Kyung-Rae Koo;Ji-min An;O-young Choi
    • Composites Research
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    • v.37 no.3
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    • pp.219-225
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    • 2024
  • It is essential to develop a light-weight, high-performance structure for the deployable reflector antenna, which is the payload of a reconnaissance satellite, considering launch and orbital operation performance. Among them, the composite main reflector is a key component that constitutes a deployable reflector antenna. In particular, the development of a high-performance main reflector is required to acquire high-quality satellite images after agile attitude control maneuvers during satellite missions. To develop main reflector, the initial design of the main reflector was confirmed considering the structural performance according to the laminate stacking design and material properties of the composite main reflector that constitutes the deployable reflector antenna. Based on the initial design, four types of composite main reflectors were manufactured with the variable for manufacturing process. As variables for manufacturing process, the curing process of the composite structure, the application of adhesive film between the carbon fiber composite sheet and the honeycomb core, and the venting path inside the sandwich composite were selected. After manufacture main reflector, weight measurement, non-destructive testing(NDT), surface error measurement, and modal test were performed on the four types of main reflectors produced. By selecting a manufacturing process that does not apply adhesive film and includes venting path, for a composite main reflector with light weight and structural performance, we developed and verified a main reflector that can be applied to the SAR(Synthetic Aperture Rader) satellite.

Development and validation of enzyme-linked immunosorbent assay for anti-mouse pertussis immunoglobulin G using international reference anti-Bordetella pertussis mouse serum NIBSC 97/642

  • Kyu-Ri Kang;Yi-Hyeon Kwon;Gyu-Won Cho;Gi-Sub Choi;Joon-Hwan Ji;Hyun-Mi Kang;Soo-Young Lee;Jin-Han Kang
    • Clinical and Experimental Vaccine Research
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    • v.13 no.3
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    • pp.242-252
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    • 2024
  • Purpose: In this study, an in-house enzyme-linked immunosorbent assay (ELISA) was developed and validated. The titer of ELISA was calculated using the reference line (RFL) method based on the standard curve drawn using the international reference anti-mouse serum NIBSC (National Institute for Biological Standards and Control) 97/642. Materials and Methods: In the development step, signal to noise was depicted to select the buffers that showed the most appropriate ratio. In the validation step, standard range, precision, dilution linearity, and specificity were confirmed, and RFL and parallel line (PLL) methods were compared in precision and dilution linearity. Results: Coating concentration for plate was achieved at 0.1 ㎍/mL for pertussis toxin (PT), 0.15 ㎍/mL for filamentous hemagglutinin antigen (FHA), and 0.25 ㎍/mL for pertactin (PRN). The signal to noise ratio was 22.02 for PT, 14.93 for FHA, and 8.02 for PRN with 0.25% goat serum in phosphate-buffered saline (PBS) as a dilution buffer, and 2% skim milk in PBS as a blocking buffer. Based on the precision results, we assessed the lower limit of quantification by 1, 0.2, and 1.5 EU/mL concentration for PT, FHA, and PRN which met the ICH (International Council for Harmonization) M10 criteria of a 25% accuracy and total error of 40%. In specificity, homologous serum was spiked into heterologous serum and the accuracy met the criteria. There was no difference in the results between RFL and PLL calculations (p-value=0.3207 for PT, 0.7394 for FHA, 0.2109 for PRN). Conclusion: ELISA validated with RFL calculation method in this study is a relatively accurate assay for mouse humoral immunogenicity test.

Changes of lateral cephalometric values according to the rotation of head (두부회전에 따른 측모두부방사선 계측치의 변화)

  • Kim, Kwang-Soo;Hwang, Mee-Sun;Choi, Eui-Hwan;Kim, Kwang-Won;Yoon, Young-Jooh
    • The korean journal of orthodontics
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    • v.30 no.1 s.78
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    • pp.53-66
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    • 2000
  • This study was performed to find out the effect of projection errors on cephalometric linear and angular measurements according to head rotation during taking lateral cephalometric radiographs. Seventeen skulls with permanent dentition and no gross asymmetry were obtained from the Department of Anatomy, Medical School, Chosun University. Total 527 x-ray films were taken with $1^{\circ}$ interval from the reference position($0^{\circ}$) to ${\pm}15^{\circ}$ around the vertical axis (Z axis) which is perpendicular to the midpoint of the line connecting the center of two ear rods in submento-vertex direction. Statistical analysis was performed by paired t-test if there were statistically significant differences between the mean of the reference position($0^{\circ}$) and that of each rotation angle. The following results were obtained. 1. The projection errors of angular measurements were smaller than those of linear measurements. 2. The projection errors of angular measurements including midline landmarks were smaller than those including bilateral landmarks. 3. The horizontal linear measurements were gradually decreased when the stroll was rotated toward the film, but slightly increased and then decreased when the skull was rotated toward the focal spot. However, the changes were smaller in focal direction. 4. The projection errors of horizontal linear measurements were larger than those of vertical linear measurements. 5. The projection errors of vertical linear measurements were increased with increased distance from the rotation axis to vertical measurements. It is concluded that the use of angular measurements rather than linear measurements is recommended to minimize the projection errors.

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