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Cytotoxicity in HL-60 cells and human lymphocytes and effect of leukemia cell differentiation induced by Saussureae Radix extract (정상사람림프구와 HL-60 cell에서 목향의 세포독성과 백혈병세포 분화효과에 관한 연구)

  • Lee, Young-Joon;Kang, Su-Jin;Ku, Sae-Kwang
    • The Korea Journal of Herbology
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    • v.26 no.2
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    • pp.31-37
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    • 2011
  • Objectives : This study was focused to investigate the toxicity of Saussurea lappa (SL) extracts in HL-60 cells and human lymphocytes. We also examined the differentiation effect of SL against leukemia cells. Methods : For examining the toxicity of SL, cytokinesis-block micronucleus (CBMN) assay and single cell gel eletrophoresis (SCGE) assay were used in present study. The cell differentiation effect of SL was evaluated by nitroblue tetrazolium (NBT) reduction assay. Results : The inhibition of cell growth in HL-60 cells was observed in a dose-dependant manner after SL treatment for 24 h. According to SCGE assay, HL-60 cells treated with SL increased DNA damage at $10{\mu}g/m{\ell}$, while DNA damage was induced by 0.1, 1, $10{\mu}g/m{\ell}$ concentration of SL in human lymphocytes. Our results indicated that SL have no genotoxic effect in HL-60 cells and human lymphocytes. Additionally, the differentiation effect was induced in $1{\mu}g/m{\ell}$ SL-treated HL-60 cells. Conclusions : From above results it is suggested that SL could be beneficial for the preparation of the useful agent for treating leukemia.

The Research about Voice Transmission between CDMA Network and PSTN Network Using CDMA Circuit Data Service (CDMA 회선 데이터 서비스를 이용한 CDMA망과 PSTN 망간의 음성 전송에 관한 연구)

  • Park, Yong-Seok;Ahn, Jae-Hwan;Ryou, Jae-Cheol
    • The KIPS Transactions:PartC
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    • v.15C no.5
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    • pp.367-374
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    • 2008
  • To realize the voice privacy between CDMA mobile phone and PSTN terminal, the voice frames shall be transmitted transparently between the heterogeneous networks. For satisfying this requirement, we propose the method which transmits voice frames using the CDMA circuit data channel in real time. In this paper we analyze the causes of voice delay which occurs during voice transmission using circuit data channel. And in order to overcome this kind of delay, the technique controlling the TCP control flag and the variable audio block construction algorithm according to the vocoder output rate are proposed. As a result of experimenting by applying the proposed method, we confirmed that the transit delay was improved with about average 70%.

A Comparison of Signal Processing Techniques in Optical Current Sensor for GIS

  • Kim, Young-Min;Park, Jung-Hwan;Jee, Seung-Wook;Lee, Kwang-Sik;Kim, Jung-Bae;Park, Won-Zoo
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.20 no.9
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    • pp.103-109
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    • 2006
  • This research is contents about output characteristic of optic current sensor that use faraday effect. optic current sensor used in an experiment is consisted of three parts.(1) Source of light used laser diode of 1310[nm].(2) Sensor section manufactured circularly according to gas insulated switchgear. And $9/125[{\mu}m]$ standard single mode optical fiber for communication was installed winding 20 [turn] on sensor section core surroundings of diameter 31 [cm].(3) Electrical signal of PD(Photo detector) is collected using NI company's 16bit DAQ board via terminal block. Collected data analyzed by different three signal processing methods. NI company's $Labview^{TM}$ was used to signal processing software. As a result, In signal processing of optic current sensor, we could know that noise greatly more influences the error generation than fluctuation of light intensity. also, 1 class CT(current transformer) manufacture that have error rate less than 1[%] was available by removing these

Production of (R)-Ethyl-4-Chloro-3-Hydroxybutanoate Using Saccharomyces cerevisiae YOL151W Reductase Immobilized onto Magnetic Microparticles

  • Choo, Jin Woo;Kim, Hyung Kwoun
    • Journal of Microbiology and Biotechnology
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    • v.25 no.11
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    • pp.1810-1818
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    • 2015
  • For the synthesis of various pharmaceuticals, chiral alcohols are useful intermediates. Among them, (R)-ethyl-4-chloro-3-hydroxybutanoate ((R)-ECHB) is an important building block for the synthesis of L-carnitine. (R)-ECHB is produced from ethyl-4-chloro-3-oxobutanoate (ECOB) by a reductase-mediated, enantioselective reduction reaction. The Saccharomyces cerevisiae YOL151W reductase that is expressed in Escherichia coli cells exhibited an enantioselective reduction reaction toward ECOB. By virtue of the C-terminal His-tag, the YOL151W reductase was purified from the cell-free extract using Ni2+-NTA column chromatography and immobilized onto Ni2+-magnetic microparticles. The physical properties of the immobilized reductase (Imm-Red) were measured using electron microscopy, a magnetic property measurement system, and a zeta potential system; the average size of the particles was approximately 1 μm and the saturated magnetic value was 31.76 emu/g. A neodymium magnet was used to recover the immobilized enzyme within 2 min. The Imm-Red showed an optimum temperature at 45℃ and an optimum pH at 6.0. In addition, Bacillus megaterium glucose dehydrogenase (GDH) was produced in the E. coli cells and was used in the coupling reaction to regenerate the NADPH cofactor. The reduction/oxidation coupling reaction composed of the Imm-Red and GDH converted 20 mM ECOB exclusively into (R)-ECHB with an e.e.p value of 98%.

Protection of the MMCs of HVDC Transmission Systems against DC Short-Circuit Faults

  • Nguyen, Thanh Hai;Lee, Dong-Choon
    • Journal of Power Electronics
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    • v.17 no.1
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    • pp.242-252
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    • 2017
  • This paper deals with the blocking of DC-fault current during DC cable short-circuit conditions in HVDC (High-Voltage DC) transmission systems utilizing Modular Multilevel Converters (MMCs), where a new SubModule (SM) topology circuit for the MMC is proposed. In this SM circuit, an additional Insulated-Gate Bipolar Translator (IGBT) is required to be connected at the output terminal of a conventional SM with a half-bridge structure, hereafter referred to as HBSM, where the anti-parallel diodes of additional IGBTs are used to block current from the grid to the DC-link side. Compared with the existing MMCs based on full-bridge (FB) SMs, the hybrid topologies of HBSM and FBSM, and the clamp-double SMs, the proposed topology offers a lower cost and lower power loss while the fault current blocking capability in the DC short-circuit conditions is still provided. The effectiveness of the proposed topology has been validated by simulation results obtained from a 300-kV 300-MW HVDC transmission system and experimental results from a down-scaled HVDC system in the laboratory.

Two-Way MIMO AF Relaying Methods Having a Legacy Device without Self-Interference Cancellation (자기간섭 제거 기능이 없는 기존 단말을 가지는 양방향 다중입출력 중계 증폭 전송 기법)

  • Lee, Kyoung-Jae
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.42 no.2
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    • pp.338-344
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    • 2017
  • In this paper, two-way amplify-and-forward relay methods are investigated where two terminals and one relay node are equipped with multiple antennas. In two-way relay channels, it is assumed that one terminal can eliminate its own self-interference but the other cannot. For this channel, we first maximize the sum-rate performance by employing an iterative gradient descent (GD) algorithm. Then, a simple singular value decomposition (SVD) based block triangularization is developed to null the self-interference. Simulation results show the proposed methods outperform the conventional schemes for various environments.

Novel Macromonomer as a Reactive Stabilizer in the Dispersion Polymerization of Methylmethacrylate

  • Jung, Hye-Jun;Lee, Kang-Seok;Shim, Sang-Eun;Yang, Sun-Hye;Lee, Jung-Min;Lee, Hui-Je;Choe, Soon-Ja
    • Macromolecular Research
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    • v.12 no.5
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    • pp.512-518
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    • 2004
  • We have synthesized a novel macromonomer of vinyl-terminated bifunctional polyurethane having a molecular weight of 37,000 g/mol and successfully applied it to the dispersion polymerization of methylmethacrylate(MMA). We verified the presence of the vinyl terminal group and the macromonomer grafted onto the poly(ethylene glycol)(PEG) block in the PMMA particles by using $^1$H and $\^$13/C NMR spectroscopies. Monodisperse PMMA microspheres that have good uniformity of 1.01 were prepared at 20 wt% macromonomer content; we investigated the characteristics of the PMMA particles in terms of their molecular weight, molecular weight distribution, size of the particles, thermal properties, and glass transition temperature. We have found that the synthesized polyurethane macromonomer is an effective stabilizer.

Implementation for Modbus/RTU Protocol Using LabVIEW (LabVIEW를 이용한 Modbus/RTU 프로토콜 구현)

  • Jeong, Tae-il;Lee, Tae-oh;Kim, Gwan-hyung;Kim, Hyun-soo;Lee, Hyung-ki
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2015.10a
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    • pp.1054-1057
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    • 2015
  • Real time processing is important in the industrial field. In this paper, we implement for Modbus/RTU(Remote Terminal Unit) protocol using LabVIEW based on serial communication. In other to obtain the experimental data from torque sense and voltage/current meter, Modbus/RTU protocol is implemented by LabVIEW tool. Mechanical loss can be calculated by torque, RPM, voltage, and current. Source code consist of panel and block diagram. We confirmed these source code can be applied in industrial field.

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Performance Analysis of Dual-Hop Cooperative Transmission with Best Relay Selection in a Rayleigh Fading Channel

  • Nessa, Ahasanun;Lee, Woo-Yong;Kim, Yong-Sun;Kwak, Kyung-Sup
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.34 no.7A
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    • pp.530-539
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    • 2009
  • Wireless Relaying is a promising solutions to overcome the channel impairments and provides high data rate coverage that appear for beyond 3G mobile communications. In this paper we present end to end BER performance of dual hop wireless communication systems equipped with multiple Decode and Forward relays over Rayleigh fading channel with the best relay selection. We compare the BER performance of the best relay with the BER performance of single relay. We select the best relay based on the end to end channel conditions. We further calculate the outage probability of the best relay. It is shown that the outage probability of the best relay is equivalent to the outage probability when all relays take part in the transmission. We apply Orthogonal Space Time Block coding(OSTBC) at the source terminal. Numerical and simulation results are presented to verify our analysis.

A Power Efficient Versatile Carry Skip Adder Architecture for the Multimode Mobile Modem (멀티모드 이동 통신 모뎀을 위한 전력 효율적 다기능 캐리스킵 가산기)

  • Han, Tae-Hee
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.45 no.3
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    • pp.86-93
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    • 2008
  • The multi-mode terminal modem which is capable of accommodating a variety of wireless communication standards needs versatile arithmetic units for processing a variety of word lengths and wide range of data rates. Since the target hardware is usually designed to meet the required highest performance, it is often wasteful in power consumption especially when low rate data processing cases. Thus, a speed and power adaptability of the arithmetic unit is a desirable feature for the wireless applications. In this paper, we propose a power efficient versatile adder architecture with carry skip logic as a basic building block constructed in hierarchical manner. The validity of the architecture is shown with respect to size, performance, and power efficiency in diverse operating modes.