• 혜화의학회지
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• 제10권1호
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• pp.237-245
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• 2001

I have come to next conclusions in consequnce of documentary study about medical books of many generations regarding Jung point venesection therapy. 1. Jung point is significant as the beginning of pulse energy flow on meridian, the origin of all meridional pulse energy being located at extrimity terminal, confluence of three yin and three yang, and emergent treatment point. 2. Jung point venesection therapy was much used for the first-aid treatment for acute and thermic disease, and that is the combination of the meaning as of restoration from death and emergent treatment of Jung point and function of openning of orifice, leakage of fever, circulation of blood, remove of edema of venesection therapy. 3. It is very much used for emergency case, five sensory organ disease and CVA, heating shock and so on. Besides that digestive disease, cough, fever with cold, childhood disease, cardiac ache, thoracic disease, numbness of digitus terminal, mental disorder follow that in order. 4. Sosang, Jung point of Arm Greater Yin Lung meridian, is very much used for five sensory organ disease. Sosang is for orbital disease, sangyang for auditory disease, sosang for nasal disease, and sosnag for introitus-throat disease. 5. Ten Jung point is most used for first-aid diseases of CVA, heating shock. 6. Ten Jung point is most used for digestive disease of stomach ache, vomiting, diarrhea, intestinal convulsion. 7. Ten Jung point is most used for cold disease of cough, fever, malaria. 8. Ten Jung point, sosang, kwanchung are much used for childhood disease of acute convulsion and fever. 9. Among Jung point, sosang is most used, 34 times. Next there are Ten Jung point, kwanchung, sangyang, sotaek, jungchung, unbaek, taedon in order. In the order of frequency in use, arm channel is more used than leg channel.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2008년도 제30회 춘계학술대회논문집
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• pp.295-298
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• 2008

램제트 추진 시스템 제어를 위한 모델링 기법에 대한 연구를 수행하였다. 연료공급시스템은 동적 메커니즘을 묘사하기 위해 전형적인 2차 시스템 모델을 사용하였으며, 이로부터 연료공급시스템의 계단 응답특성을 계산하고 이에 상응되는 연소실 압력섭동과 종말 충격파의 위치 변화 관계를 파악하였다. 압력섭동과 종말 충격파 간의 상관관계를 도출하기 위하여 연소실 압력은 일정한 주기와 진폭을 갖는 사인파로 모델링하여 종말충격파의 거동에서 나타나는 위상차를 확인하였다. 본 연구를 통해 시스템의 작동에 대한 물리적인 이해를 도모하며, 시스템의 동적 특성이 엔진 작동에 미치는 영향을 살펴보았다.

• 대한한방부인과학회지
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• 제21권2호
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• pp.152-165
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• 2008

Purpose: It is the purpose of this study to investigate the anti-inflammatory effects and mechanism of cheukbaekjurpihwan(CBJPH) extract on LPS (lipopolysaccharide)-induced inflammatory mediators in murine peritoneal macrophages. Methods: To evaluate anti-inflammatory effects of CBJPH extract, the production of cytokines(TNF-${\alpha}$(tumor necrosis factor-alpha), IL(interleukin)-6, IL-12) and NO(nitric oxide) was measured in vitro and in vivo. And western blot analysis has been done to look into the mechanism. Results: CBJPH extract reduced LPS-induced NO, TNF-${\alpha}$ and IL-6, IL-12 productions in peritoneal macrophages. CBJPH extract inhibited the activation of JNK(c-Jun N-terminal kinase), but didn't inhibit the activation of MAPKs (mitogen-activated protein kinases) such as p38, ERK1/2(extracelluar signal-regulated kinase1/2) and the degradation of $I_{\kappa}B-{\alpha}$(inhibitory kappa B-alpha) in the LPS-stimulated peritoneal macrophages. CBJPH extract suppressed LPS-induced endotoxin shock and the productions of TNF-${\alpha}$, but not of IL-6, after an oral administration of CBJPH extract Conclusion: CBJPH extract suppressed the productions of LPS-induced NO and cytokines by preventing JNK from phosphorylation, which may provide a clinical basis for anti-inflammatory properties of CBJPH.

• 대한미생물학회지
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• 제35권2호
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• pp.109-115
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• 2000

FtsH is a membrane-bound, ATP-dependent metalloprotease that is involved in a variety of cellular functions including the regulation of responses to heat and stress shock. Previously, we had cloned and sequenced pneumococcal ftsH gene whose deduced amino acid sequence was very similar to those of several gram-positive bacteria and Escherichia coli, except for the N-terminal domain that was responsible for membrane anchoring. In order to better understand the role of Streptococcus pneumoniae FtsH, we expressed pneumococcal ftsH gene in Escherichia coli. When it was expressed from a strong promoter, $P_{tac}$, a considerable amount of the recombinant FtsH was produced, although the prolonged induction resulted in not only accumulation of breakdown products but also ceasing of the further growth of E. coli host. This indicated that the expression of the exogenous ftsH gene was tightly regulated since the excessive FtsH appeared detrimental to bacterial cells. In Western blotting, the pneumococcal FtsH protein, whether native or recombinant, was reactive to anti-E. coli FtsH serum. The observation that FtsH proteins were well conserved throughout the bacterial kingdom and its expression level was fine-tuned suggests an important role for this protein in the stress adaptation which may be related to infecting process by pneumococci.

• 한국결정학회:학술대회논문집
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• 한국결정학회 2002년도 정기총회 및 추계학술연구발표회
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• pp.24-24
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• 2002

HtrA (high temperature requirement A), a periplasmic heat shock protein, is known to have molecular chaperone function at low temperatures and proteolytic activity at elevated temperatures. To investigate the mechanism of functional switch to pretense, we have determined the crystal structure of the N-terminal protease domain (PD) of HtrA from Thermotoga maritima. HtrA PD shares the same fold with chymotrypsin-like serine professes. However, crystal structure suggests that HtrA PD is not an active pretense at current state since its active site is not formed properly and blocked by an additional helical lid. On the surface of the lid, HtrA PD has hydrophobic patches that could be potential substrate binding sites for molecular chaperone activity. Present structure suggests that the activation of the proteolytic function of HtrA PD at elevated temperatures might occur by the conformational change.

• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1353-1360
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• 2007

Three csp-like genes were identified in the Corynebacterium glutamicum genome and designated cspA, cspB, and cspA2. The genes cspA and cspA2 encode proteins, comprising of 67 amino acid residues, respectively. They share 83% identity with each other. Identity of those proteins with Escherichia coli Csp proteins was near 50%. The cspB gene encodes a protein composed of 127 amino acids, which has 40% and 35% sequence identity with CspA and CspA2, respectively, especially at its N-terminal region. Analysis of the gene expression profiles was done using transcriptional cat fusion, which identified not only active expression of the three genes at the physiological growth temperature of $30^{\circ}C$ but also growth phase-dependent expression with the highest activity at late log phase. The promoters of cspA and cspA2 were more active than that of cspB. The expression of the two genes increased by 30% after a temperature downshift to $15^{\circ}C$, and such stimulation was more evident in the late growth phase. In addition, the cspA gene appeared to show DNA-binding activity in vivo, and the activity increased at lower temperatures. Interestingly, the presence of cspA in multicopy hindered the growth of the host C. glutamicum cells at $20^{\circ}C$, but not at $30^{\circ}C$. Altogether, these data suggest that cspA, cspB, and cspA2 perform functions related to cold shock as well as normal cellular physiology. Moreover, CspA and its ortholog CspA2 may perform additional functions as a transcriptional regulator.

• 한국동물학회지
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• 제33권2호
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• pp.191-199
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• 1990

토끼의 골격근에서 근소포체를 순수 분리하여 SDS-polyacrylamide gel전기영동법과 125 I-concanavalin A표지법으로 단백질과 당단백질의 조성을 조사하였다. 전기영동사에 나타난 대표적인 단백질은 $Ca^2$+-AThase, 80 Kd protein,calsequestrin,high affinity calcium binding protein, intrinsic glycoprotein이었으며, 160 Kd protein, 94 Kd protein,38 Kd protein, 34 Kd protein,24 Kd proteins도 존재하였다.특히, 막성계에 있는 heak protein으로 알려져 있는 80 Kd protein은 본 연구를 통해 주로 근소포체의 terminal cisternae에 들어 있음이 확인되었다. 한편 125 I-concanavalin A표지에 의해 전기영동성에 나타난 대표적인 당단백질은 160 Kd glycoprotein, 94 Kd glycoprotein, calsequestrin, intrinsic glycoprotein의 4종이었다. 이 가운데 94 Kd glycoprotein은 94 Kd glucose-regulated protein으로 추정되며, 본 연구를 통해 근소포체에서도 특히 T-tubule에 다량으로 존재함이 밝혀졌다.

• 미생물학회지
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• 제30권6호
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• pp.421-424
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• 1992

Bacillus megaterium ATCC14945의 페니실린 지 아실라제 유전자를 갖는 Escherichia coli JM83이 한 종류의 단백질을 대량으로 만들어 내었다(전체 세포 단백질 양의 20%이상). 이 단백질은 아미노 말단의 아미노산 서열 분석을 통해서 E. coli heat protein인 GroEL로 확인되었다. 또한 이 groEL 단백질은 외래의 페니실린 지 아실라제가 발현됨으로써 27과 37도 두 온도에서 생산됨을 알았다.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2006년도 추계 학술대회논문집
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• pp.185-188
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• 2006

An investigation of the ejector-jets focusing on its flow properties was carried out by varying the geometric parameters. The area ratio of the primary nozzle, AR that was tested in the present measurement was 2.17 and 3.18, while the ratio of the length to the diameter of the duct downstream the primary nozzle inlet, L/D had values of 3.41, 6.82, and 10.23. For the frame work of flow characteristics, the wall pressures distribution of ejector-jet was investigated by experiment for basic study of ejector-jet performance. In result, terminal shock location and existence of series of oblique shocks are recognized. In this study, CFD analysis was conducted at the same time. And as a result of comparison experiment data with CFD analysis, the physical phenomena of ejector-jets were estimated.

• 한국추진공학회지
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• 제4권1호
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• pp.74-92
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• 2000

A numerical analysis has been conducted to study the transient flowfield during the transition from the booster to sustainer phase in an integrated rocket ramjet (IRR) propulsion system. Emphasis is placed on the unsteady inlet aerodynamics, fuel/air mixing in an entire ramjet engine during the flow transient phase. The computational geometry consists of the entire IRR engine, including the inlet, the combustion chamber, and the exhaust nozzle. Turbulence closure is achieved using a low-Reynolds-number two-equation model. The governing equations are solved numerically by means of a finite-volume, preconditioned flux-differencing scheme over a wide range of Mach umber. Various important physical processes are investigated systemically, including terminal shock train.