• Journal of Korean Critical Care Nursing
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• v.13 no.1
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• pp.76-86
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• 2020

Purpose : The purpose of this study was to identify the extent to which nurses' death perceptions, death anxiety, and stress associated with terminal care affects their attitudes toward terminal care. Method : A total of 214 nurses were asked questions about characteristics, death perceptions, death anxiety, stress associated with end-of-life care, and terminal care attitudes, using structured questionnaires. The data were analyzed using descriptive analysis, t-test, ANOVA, Scheffé's test, Pearson's correlation coefficients, and stepwise multiple regression. Results : Factors influencing terminal care attitudes in participants were positive meaning associated with death, respect for life, final education, end-of-life nursing education experiences, stress associated with end-of-life care, death anxiety, and position. These factors explained 38.7% of variance in participants' terminal care attitudes (F=20.18, p<.001). Conclusion : In order for nurses to have a positive attitude toward terminal care, it is necessary to raise positive awareness about death and respect for life, and it is necessary to develop various educational programs and strengthen their capacity through continuous education.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.41-41
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• 2003

Small conductance calcium-activated potassium channels (or SKCa channels) are potassium selective, voltage-independent, and activated by intracellular calcium concentration. These channels play important roles in excitable cells such as neuron in the central nervous system (Vergara et al., 1998). The activity of SKCa channels underlies the slow afterhyperpolarization that inhibits neuronal cell firing (Hille, 1991; Vergara et al.,1998). Until now, N-terminal region of rSK2 isn't characterized. To study the role of N-terminus, we constructed the N-terminal deletion mutant and characterized by electrophysiological means. Interestingly, N-terminal deletion mutant be trafficked to membrane couldn't evoke any ionic currents. Thus, N-terminal region has a role in functional rSK2 channel formation. To elucidate the function of N-terminal region, (His)6-conjugated protein was purified and filtrated by affinity column chromatography. Surprisingly, N-terminal region was shown in tetramer size that was supported by cross-linking result. Thus, we predicted that N-terminal region might be involved in the tetramerization of rSK2.

• Biomedical Science Letters
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• v.9 no.2
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• pp.105-110
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• 2003

Ankyrins are a ubiquitously expressed family of intracellular adaptor proteins involved in targeting diverse proteins to specialized membrane domains in both the plasma membrane and the endoplasmic reticulum. Canonical ankyrins are 190-220 kDa proteins expressed in most tissues and cell types and comprise a membrane-binding domain (MBD) of 24 ANK repeats, a spectrin-binding domain, a death domain and a C-terminal domain. Rescue studies with ankyrin-B/G chimeras have identified the C-terminal domain of ankyrin-B as the defining domain in specifying ankyrin-B activity, but the function of C-terminal domain of ankyrin-B is, however, not known. We report here that the C-terminal domain of ankyrin-B is capable of interacting with the C-terminal Region of Hsp40. The Hsps are induced not only by heat shock but also by various other environmental stresses. Hsps are also expressed constitutively at normal growth temperatures and have basic and indispensable functions in the life cycle of proteins as molecular chaperones, as well as playing a role in protecting cells from the deleterious stresses. The binding sites required in the interaction between C-terminal domain of ankyrin-B and C-terminal region of Hsp40 were characterized using the yeast two-hybrid system and GST-pull down assay. The interaction between ankyrin-B and Hsp40 represents the first direct evidence of ankyrin's role as chaperones.

• Journal of Korea Port Economic Association
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• v.21 no.1
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• pp.59-72
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• 2005

There were many theoretical studies using mathematical models about a yard storage capacity in a container terminal so far, but a simulation approach is newly popularizing. The reason why the simulation studies about yard storage capacity were a few was that once the most important part in a container terminal was a quay part. However, from the economic crisis year of 1977, the yard storage part in a container terminal became a critical resource because of the shortage of SOC investment resources. Therefore, after discharging or loading even through there was a waiting in the quay part or not, it can be swiftly improved the efficiency of a container terminal if it was handled rapidly or smoothly in a container yard. So the accurate assessment of yard storage capacity in a container terminal was needed. This study planed to assess the operation capability of a container yard via a simulation model. The model included many chatacteristics of three Korean container terminals such as Gamman Hanjin, Uam, and Hutchinson Busan at the period of 1999 to 2000. The 95% percentile was chosen as a criterion for judging of the storage capability by the recommendation of KPC (1998) and JWD (1998). A simulation approach with system dynamics concept considering the multi-directional impacts within the related variables can probavly foresee the future storage capacity of a terminal not just the past.

• Journal of the Korea Society of Computer and Information
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• v.22 no.6
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• pp.115-122
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• 2017

The purpose of this study was to investigate the effects of compassion satisfaction, compassion fatigue, burnout on the attitude towards terminal care among care providers in long-term care facilities. In order to achieve the purpose of the study, data was collected from 126 care providers in long-term care facilities in Chungbuk and Kangwon province. Data were collected with self-administrated questionnaires and analyzed by hierarchical multiple regression. Findings of this study is as follows. Firstly, attitude towards terminal care was positively affected by compassion satisfaction. Secondly, the attitude towards terminal care was negatively affected by compassion fatigue. Thirdly, the attitude towards terminal care was negatively affected by burnout. In conclusion, these findings provide strong empirical evidence for the importance of compassion fatigue, compassion satisfaction and burnout in explaining attitude towards terminal care among care providers in long-term care facilities. Therefore, strategies to decrease compassion fatigue and burnout and improve compassion satisfaction are needed to improve the attitude towards terminal care among care providers in long-term care facilities.

• BMB Reports
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• v.52 no.5
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• pp.330-335
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• 2019

Hepatitis B virus (HBV) encoding the HBV x protein (HBx) is a known causative agent of hepatocellular carcinoma (HCC). Its pathogenic activities in HCC include interference with several signaling pathways associated with cell proliferation and apoptosis. Mutant C-terminal-truncated HBx isoforms are frequently found in human HCC and have been shown to enhance proliferation and invasiveness leading to HCC malignancy. We investigated the molecular mechanism of the reduced doxorubicin cytotoxicity by C-terminal truncated HBx. Cells transfected with C-terminal truncated HBx exhibited reduced cytotoxicity to doxorubicin compared to those transfected with full-length HBx. The doxorubicin resistance of cells expressing C-terminal truncated HBx correlated with upregulation of the ATP binding cassette subfamily B member 1(ABCB1) transporter, resulting in the enhanced efflux of doxorubicin. Inhibiting the activity of ABCB1 and silencing ABCB1 expression by small interfering ribonucleic acid (siRNA) increased the cytotoxicity of doxorubicin. These results indicate that elevated ABCB1 expression induced by C-terminal truncation of HBx was responsible for doxorubicin resistance in HCC. Hence, co-treatment with an ABCB1 inhibitor and an anticancer agent may be effective for the treatment of patients with liver cancer containing the C-terminal truncated HBx.

• Korean Journal of Occupational Health Nursing
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• v.28 no.4
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• pp.230-241
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• 2019

Purpose: This study aimed to investigate the correlation between death anxiety, terminal care stress, and job satisfaction of new nurses, as well as to identify factors affecting job satisfaction using descriptive correlations. Methods: This study included 143 new nurses who had 3 to 12 months of experience in terminal care. Data were collected from January to February 2018, and were analyzed using descriptive statistics, t-test, ANOVA, Scheffe? test, Pearson's correlation coefficient, and hierarchical regression analysis. Results: There was a negative correlation between job satisfaction and terminal care stress (r=-.170, p=.043), while death anxiety and terminal care stress were positively correlated (r=.284, p=.001). The following findings demonstrated a significant effect on job satisfaction: lesser the clinical career experience, the lower the job load causing death anxiety and terminal care stress, and the higher the job satisfaction. Furthermore, the explanatory power of these factors was 15.1%. Conclusion: To assist new nurses within three months of joining in clinical adaptation, it is necessary to provide them with appropriate knowledge regarding terminal care through training, and with counseling opportunities for the psychological burdens they experience while caring for dying patients.

• BMB Reports
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• v.56 no.3
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• pp.172-177
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• 2023

BEST family is a class of Ca²⁺-activated Cl- channels evolutionary well conserved from bacteria to human. The human BEST paralogs (BEST1-BEST4) share significant amino acid sequence homology in the N-terminal region, which forms the transmembrane helicases and contains the direct calcium-binding site, Ca²⁺-clasp. But the cytosolic C-terminal region is less conserved in the paralogs. Interestingly, this domain-specific sequence conservation is also found in the BEST1 orthologs. However, the functional role of the C-terminal region in the BEST channels is still poorly understood. Thus, we aimed to understand the functional role of the C-terminal region in the human and mouse BEST1 channels by using electrophysiological recordings. We found that the calcium-dependent activation of BEST1 channels can be modulated by the C-terminal region. The C-terminal deletion hBEST1 reduced the Ca²⁺-dependent current activation and the hBEST1-mBEST1 chimera showed a significantly reduced calcium sensitivity to hBEST1 in the HEK293 cells. And the C-terminal domain could regulate cellular expression and plasma membrane targeting of BEST1 channels. Our results can provide a basis for understanding the C-terminal roles in the structure-function of BEST family proteins.

• Journal of Korean Public Health Nursing
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• v.37 no.2
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• pp.275-289
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• 2023

Purpose: This study developed a structural model for explaining and predicting terminal care performance in long-term care hospital nurses. The model was based on the stress integration model of Ivancevich and Matteson(1980) and the results of previous studies. Method: Data was obtained from August to September 2022 from 267 nurses in 13 long-term care hospitals in G-do. Results: Results of model verification for this study, revealed that factors directly affecting the terminal care performance of long-term care hospital nurses were nursing work environment(β=0.43, p<0.001), death anxiety(β=-0.29, p<0.001), and terminal care stress(β=0.22, p=0.003). However, the attitude toward nursing care of dying(β=0.07, p=0.287) had no effect on the terminal care performance. Conclusion: The results of this study, confirmed the necessity of improving an individual's perceived nursing work environment, continuous education related to terminal care to reduce death anxiety, and an interventional approach for enhancing terminal care performance.

• Korea Trade Review
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• v.45 no.6
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• pp.45-54
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• 2020

Occurrence of accidents at a container terminal results in casualties and damages of equipments which are involved in the accidents, and affects the terminal operation. Therefore, analyzing factors affecting occurrence of accidents at a container terminal is important for efficient operation of the terminal. Most of existing studies analyzing factors of accidents have performed in a land transportation field. And most of existing studies in a maritime field have focused on developing risk assessment methods, rather than identifying factors of accidents. This study aims to analyze the factors affecting the damage level which was resulted from accidents at a container terminal in Busan, Korea. After a basic statistical analysis of the accident dataset which was occurred in the terminal, a linear regression model is applied to identify the factors which affect the damage level resulted from the accident. As a result of analysis, it is found that the more number of equipments, facilities and containers damaged from the accidents positively affect the damage level from the accidents, as well as dozing during working. The results of this study are expected to be a basic for developing safety management.