• Title/Summary/Keyword: Technology intensity of sectors

Search Result 18, Processing Time 0.025 seconds

Optimization of a microarray for fission yeast

  • Kim, Dong-Uk;Lee, Minho;Han, Sangjo;Nam, Miyoung;Lee, Sol;Lee, Jaewoong;Woo, Jihye;Kim, Dongsup;Hoe, Kwang-Lae
    • Genomics & Informatics
    • /
    • v.17 no.3
    • /
    • pp.28.1-28.9
    • /
    • 2019
  • Bar-code (tag) microarrays of yeast gene-deletion collections facilitate the systematic identification of genes required for growth in any condition of interest. Anti-sense strands of amplified bar-codes hybridize with ~10,000 (5,000 each for up-and down-tags) different kinds of sense-strand probes on an array. In this study, we optimized the hybridization processes of an array for fission yeast. Compared to the first version of the array (11 ㎛, 100K) consisting of three sectors with probe pairs (perfect match and mismatch), the second version (11 ㎛, 48K) could represent ~10,000 up-/ down-tags in quadruplicate along with 1,508 negative controls in quadruplicate and a single set of 1,000 unique negative controls at random dispersed positions without mismatch pairs. For PCR, the optimal annealing temperature (maximizing yield and minimizing extra bands) was 58℃ for both tags. Intriguingly, up-tags required 3× higher amounts of blocking oligonucleotides than down-tags. A 1:1 mix ratio between up- and down-tags was satisfactory. A lower temperature (25℃) was optimal for cultivation instead of a normal temperature (30℃) because of extra temperature-sensitive mutants in a subset of the deletion library. Activation of frozen pooled cells for >1 day showed better resolution of intensity than no activation. A tag intensity analysis showed that tag(s) of 4,316 of the 4,526 strains tested were represented at least once; 3,706 strains were represented by both tags, 4,072 strains by up-tags only, and 3,950 strains by down-tags only. The results indicate that this microarray will be a powerful analytical platform for elucidating currently unknown gene functions.

Magnetic-field-tuned Insulator to Conductor Transition in Magnetorheological Suspension

  • Yang, Xiongbo;Jiang, Yuhuan;Huang, Yuehua;Xu, Ruizhen;Piao, Hongguang;Jia, Gaomeng;Tan, Xinyu
    • Journal of Magnetics
    • /
    • v.19 no.4
    • /
    • pp.345-348
    • /
    • 2014
  • Magnetorheological suspensions (MRSs) are smart materials that have the potential to revolutionize several industrial sectors because of their special rheological behaviors. In this paper, MRS, based on carbonyl iron (CI) microparticles that were dispersed in silicone oil with oleic acid, were prepared. We showed that the electroconductibility of MRS was significantly influenced by the intensity of the external magnetic field that was applied. The resistance value can vary from infinite to below $300{\Omega}$ after applying an external magnetic field. The results indicated that this MRS had the property of magnetic-field-tuned insulator to conductor transition. This system has potential applications in controllable MRS electrical devices.

Designation of SMEs-Suitable Industry and SMEs' Performance: Evidence from Food Product and Beverages Industry (중소기업 적합업종 지정제도가 중소기업 경영성과에 미친 영향 분석: 음식료품 제조업을 중심으로)

  • Kwak, Kiho
    • Korean small business review
    • /
    • v.41 no.2
    • /
    • pp.25-50
    • /
    • 2019
  • Although Korean government has implemented size-dependent policy, so called "the designation of SMEs-suitable industry", promoting SMEs growth, our understanding in the effectiveness of the policy is limited. We investigate the effect of the policy on SMEs performance in food product and beverages industry, which accounts for the majority in the SMEs-suitable industry. From the perspective of sales, profitability, and R&D intensity, which is regarded as indigenous effort for growth, we find the heterogeneity in the effectiveness of the policy across the sub-sectors in the industry. However, overall the policy does not significantly contribute to the growth of sales, profitability, and facilitation of R&D activity for indigenous innovative efforts of SMEs. Our study advances the theoretical discussion on the effect of the policy with the disaggregated level of analysis, i.e, sub-sector level. Our findings also contribute to the resolution of social and political conflicts between pros and cons of the policy. Our study suggests that policy makers should develop more sophisticated policy that incorporate the specific characteristics of individual sub-sectors. They also need to invest more resources in enhancing the effectiveness of the policy and accelerating SMEs innovative efforts.

The Impact of Enterprise R&D Investment on Inter-industry Technology Spillover in Korea under the new Normal Era (뉴 노멀 시대하 한국기업의 R&D투자가 산업간 기술파급에 미치는 영향)

  • Kim, Seon Jae;Lee, Younghwa
    • The Journal of the Korea Contents Association
    • /
    • v.13 no.2
    • /
    • pp.390-399
    • /
    • 2013
  • The purpose of this study is to examine the impact of enterprise R&D investment on inter-industry technology spillover in Korea under the new Normal Era. In order to do this, we tested the effect of production inducement, backward and forward linkage effects, and the effect of technology spillover such as technology intensity effects and technology diffusion effects based on an input-output framework. The results show that the high index industries of the production inducement effect were professional, scientific and technical activities, manufacture of metal products, and general machinery. Some manufacturers of other non-metallic mineral products and transport equipment sectors appeared to have the strong effect of forward and backward linkages that were almost equivalent to high-tech manufacturing industries. In particular, the industries of professional, scientific and technical activities appeared to have the strong effect of both forward and backward linkages. Therefore, we need to drive a strong policy support to higher enterprise R&D investment in the those particular industries not only to increase global competitiveness, but also to widen up the technology spillover effect on other industries.

The Effect of Startups' Trust in Government R&D Policies on Innovation Performance (벤처기업의 정부 R&D정책에 대한 신뢰가 혁신 성과에 미치는 영향)

  • Kim, Deokyong;Bae, Sung Joo
    • Journal of Technology Innovation
    • /
    • v.29 no.4
    • /
    • pp.95-124
    • /
    • 2021
  • Startups play an important role in economic growth and job creation in niche markets, thus governments regularly expand their research and development(R&D) budgets accordingly. As stratups are more dependent on governments to compensate for insufficient resources and capabilities than large and medium-sized companies, trust in government policies will be important. In this study, we analyzed the impact of startups' trust in government R&D policies on innovation performance. There were three major results. The first result is that government R&D investments in startups increase innovation performance in the manufacturing and high-technology industries but did not affect other industries. The second result is that trust in government R&D policies increased innovation in high-and medium-high technology and manufacturing industries. The third result is that trust in government R&D policies affects innovation before, during, and after evaluation of support process. We analyzed the importance of trust to the effectiveness of government R&D support to determine how to effectively provide it. The results show that governments need to differentiate the types of R&D support they provide according to the target firm's technology level and whether they are a manufacturing company and that appropriate R&D support mechanism should be developed for low-technology and non-manufacturing companies. Finally, governments should allocate resources and make fair and transparent decisions to help companies grow, not to better supervise them.

Analysis of Enactment and Utilization of Korean Industrial Standards(KS) by Time Series Data Mining (시계열 자료의 데이터마이닝을 통한 한국산업표준의 제정과 활용 분석)

  • Yoon, Jaekwon;Kim, Wan;Lee, Heesang
    • Journal of Technology Innovation
    • /
    • v.23 no.3
    • /
    • pp.225-253
    • /
    • 2015
  • The standard is a nation's one of the most important industrial issues that improve the social and economic efficiency and also the basis of the industrial development and trade liberalization. This research analyzes the enactment and the utilization of Korean industrial standards(KS) of various industries. This paper examines Korean industries' KS utilization status based on the KS possession, enactments and inquiry records. First, we implement multidimensional scaling method to visualize and group the KS possession records and the nation's institutional issues. We develop several hypothesis to find the decision factors of how each group's KS possession status impacts on the standard enactment activities of similar industry sectors, and analyzes the data by implementing regression analysis. The results show that the capital intensity, R&D activities and sales revenues affect standardization activities. It suggests that the government should encourage companies with high capital intensity, sales revenues to lead the industry's standard activities, and link the policies with the industry's standard and patent related activities from R&D. Second, we analyze the impacts of each KS data's inquiry records, the year of enactments, the form and the industrial segment on the utilization status by implementing statistical analysis and decision tree method. The results show that the enactment year has significant impact on the KS utilization status and some KSs of specific form and industrial segment have high utilization records despite of short enactment history. Our study suggests that government should make policies to utilize the low-utilized KSs and also consider the utilization of standards during the enactment processes.

Estimation of willingness to pay of workers who are engaged in nuclear power R&D projects to avoid exposure to radioactive matters by using a choice experiment (선택실험설문에 의한 방사능 피폭 가능성에 대한 원자력 기술개발 종사자의 지불용의액 추정)

  • Bae, Jeong Hwan
    • Environmental and Resource Economics Review
    • /
    • v.22 no.3
    • /
    • pp.411-435
    • /
    • 2013
  • Since catastrophe from explosion of Hukushima nuclear power plant, concerns over possibility of exposure to radioactive matter has been disseminating all over the world and frequent accidents of domestic nuclear power plants also has been amplifying throughout the nation. In the past, major focus was made on compensation for local residents who live nearby nuclear power plants, but focal point of this study is on wage premium of workers who are employed in R&D of nuclear power plants. It is difficult to derive socially desirable result if private sectors are responsible for compensation on workers who suffer from physical damages due to the exposure to radioactive matter. Because victims should verify the damages that occur in the working places. This study conducted a survey on which job would prefer the respondents who are engaged with the nuclear R&D projects as exposure levels to radioactive matter, security of job, location of firms, and work intensity differ. As a result, exposure to radioactive matter was the most important attribute in choosing alternative jobs followed by job security, work intensity and job location. Annual willingness to pay for reduction of exposure to radioactive matter was estimated as 7730~7770 thousand KRW depending on different econometric models. Therefore, Korean government should prepare institutional foundation in order that appropriate compensation should be made on workers who are engaged in R&D projects on nuclear power plants if they have damages from the exposure to radioactive matter.

An Efficient Estimation of Place Brand Image Power Based on Text Mining Technology (텍스트마이닝 기반의 효율적인 장소 브랜드 이미지 강도 측정 방법)

  • Choi, Sukjae;Jeon, Jongshik;Subrata, Biswas;Kwon, Ohbyung
    • Journal of Intelligence and Information Systems
    • /
    • v.21 no.2
    • /
    • pp.113-129
    • /
    • 2015
  • Location branding is a very important income making activity, by giving special meanings to a specific location while producing identity and communal value which are based around the understanding of a place's location branding concept methodology. Many other areas, such as marketing, architecture, and city construction, exert an influence creating an impressive brand image. A place brand which shows great recognition to both native people of S. Korea and foreigners creates significant economic effects. There has been research on creating a strategically and detailed place brand image, and the representative research has been carried out by Anholt who surveyed two million people from 50 different countries. However, the investigation, including survey research, required a great deal of effort from the workforce and required significant expense. As a result, there is a need to make more affordable, objective and effective research methods. The purpose of this paper is to find a way to measure the intensity of the image of the brand objective and at a low cost through text mining purposes. The proposed method extracts the keyword and the factors constructing the location brand image from the related web documents. In this way, we can measure the brand image intensity of the specific location. The performance of the proposed methodology was verified through comparison with Anholt's 50 city image consistency index ranking around the world. Four methods are applied to the test. First, RNADOM method artificially ranks the cities included in the experiment. HUMAN method firstly makes a questionnaire and selects 9 volunteers who are well acquainted with brand management and at the same time cities to evaluate. Then they are requested to rank the cities and compared with the Anholt's evaluation results. TM method applies the proposed method to evaluate the cities with all evaluation criteria. TM-LEARN, which is the extended method of TM, selects significant evaluation items from the items in every criterion. Then the method evaluates the cities with all selected evaluation criteria. RMSE is used to as a metric to compare the evaluation results. Experimental results suggested by this paper's methodology are as follows: Firstly, compared to the evaluation method that targets ordinary people, this method appeared to be more accurate. Secondly, compared to the traditional survey method, the time and the cost are much less because in this research we used automated means. Thirdly, this proposed methodology is very timely because it can be evaluated from time to time. Fourthly, compared to Anholt's method which evaluated only for an already specified city, this proposed methodology is applicable to any location. Finally, this proposed methodology has a relatively high objectivity because our research was conducted based on open source data. As a result, our city image evaluation text mining approach has found validity in terms of accuracy, cost-effectiveness, timeliness, scalability, and reliability. The proposed method provides managers with clear guidelines regarding brand management in public and private sectors. As public sectors such as local officers, the proposed method could be used to formulate strategies and enhance the image of their places in an efficient manner. Rather than conducting heavy questionnaires, the local officers could monitor the current place image very shortly a priori, than may make decisions to go over the formal place image test only if the evaluation results from the proposed method are not ordinary no matter what the results indicate opportunity or threat to the place. Moreover, with co-using the morphological analysis, extracting meaningful facets of place brand from text, sentiment analysis and more with the proposed method, marketing strategy planners or civil engineering professionals may obtain deeper and more abundant insights for better place rand images. In the future, a prototype system will be implemented to show the feasibility of the idea proposed in this paper.