• Korean Chemical Engineering Research
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• 제56권2호
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• pp.240-244
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• 2018

There is growing concern about cross infection among the patients to patients, patients to staffs, and tools to patients in healthcare facilities, especially in dentistry. In this study, the most widely used dental impression materials were prepared and the synergy effect of Chlorhexidine and essential oil on antimicrobial activity was examined in the impression materials. Chlorhexidine concentration of 0.1 wt% and 0.5 wt% showed no antimicrobial activity on Escherichia coli (E. coli) and Candida albicans. At 1.0 wt% Chlorhexidine, 0% of E. coli and 34.7% of Candida albicans were survived. Bergamot (Essential oil) concentration of 0.5 wt% and 1.0 wt% showed no antimicrobial activity on E. coli. At 2.0 wt% Bergamot oil, 71.9% of E. coli were survived. Tea tree oil (Essential oil) of 0.5 wt% showed no antimicrobial activity on E. coli. At 1.0 wt% Tea tree oil, 11.2% of E. coli was survived. At 2.0 wt% Tea tree oil, no E. coli was survived. However, no E. coli was survived at the concentration of 0.8 wt% Bergamot with 0.3 wt% Chlorhexidine. At the concentration of 0.8 wt% Tea Tree oil with 0.3 wt% Chlorhexidine, 1.3% of E. coli were survived. The experimental results showed that the synergy effects between Chlorhexidine and essential oils on antimicrobial activity were prominent.

• Journal of Microbiology and Biotechnology
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• 제17권11호
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• pp.1848-1855
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• 2007

Antibacterial activity of essential oils (Tea tree, Chamomile, Eucalyptus) on Staphylococcus aureus growth was evaluated as well as the essential oil-loaded alginate beads. The binding interactions between the cell and the essential oils were measured using an optical biosensor. The antibacterial activity of the essential oils to the cell was evaluated with their binding interaction and affinity. The antibacterial activity appeared in the order of Tea Tree>Chamomile>Eucalyptus, in comparison of the inhibition effects of the cell growth to the essential oils. The association rate constant and affinity of the cell binding on Tea Tree essential oil were $5.0{\times}10^{-13}\;ml/(CFU{\cdot}s)$ and $5.0{\times}10^5\;ml/CFU$, respectively. The affinity of the cell binding on Tea Tree was about twice higher than those on the other essential oils. It might be possible that an effective antibacterial activity of Tea Tree essential oil was derived from its strong adhesive ability to the cell, more so than those of the other essential oils.

• 한국식품위생안전성학회지
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• 제15권1호
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• pp.55-59
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• 2000

Bamboo oil의 천연 보존료로서의 가치를 평가하기 위해 합성 보존료인 BHA와 이미 수입되어 사용되고 있는 천연보존료인 tea tree oil을 비교하기 위하여 disc diffusion법과 broth dilution 법으로 S. aureus와 E. coli대한 항균력을 검토한 결과를 요약하면 다음과 같다. 1. Disc diffusion법으로 S. aureus에 대한 항균력을 조사한 결과 BHA>tea tree oil>bamboo oil 순이었고 E. coli에 대한 항균력은 bamboo oil이 가장 강하게 나타났으며 다음은 BHA>tea tree oil순으로 나타났다. 2. Broth dilution법으로 S. aureus와 E. coli모든 시료 6.0$\mu$l/ ml 이상에서 모두 측정되지 않아 모든 시료에서 동일하게 최소억제농도가 6.0$\mu$l/ ml 이상 필요한 것으로 나타났다. 3. Bamboo oil의 이용 개발 시 경제적 조건은 동일한 항균력을 기대할 때 tea tree oil에 비하여 약 20%정도 저렴하여 경제성이 충분히 있는 것으로 나타났다.

• 융합정보논문지
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• 제11권6호
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• pp.198-205
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• 2021

본 배경 : 질염은 항생제를 복용하는 방식으로 치료를 하고 있으며, 이러한 항생제의 지속적인 치료는 내성을 유발할 수 있다. 연구 방법 : Lactic acid bacteria 2종에 에센셜 오일을 이용한 항균 활성을 보고자 한다. SE(Grapefruit Seed Extract), eucalyptus, tea tree, clove bud oil, cinnamon oil, lemongrass oil, thyme oil, ginger oil을 일정 비율로 넣어 배양 후, 병원성 미생물- E. coli, C. albicans와 Lactic acid bacteria은 균주에 맞는 배지를 사용하여 균 수를 측정하였다. 결과 : Essential oil 7종과 GSE가 병원성 미생물에 억제 효과가 있으며, 병원성균(E. coli, C. albicans)에 대한 Grapefruit seed extract(GSE)의 억제농도를 확인하였다, 병원성균은 억제하고 Lactic acid bacteria는 억제하지 않는 배합비도 확인하였다(GSE 농도가 200ppm에서 Eucalyptus globulus(EG) oil 50㎕와 Melaleuca alternifolia oil(Tea tree oil, TTO) 50㎕(pH 5.0, 5.5, 6.0)). 결론 : 본 실험에서 Essential oil은 다양한 항균 활성 가지고 있어 항생제 대안으로도 생각할 수 있으며, 장기 항생제 치료환자에 대한 보조 항균제로서도 유용할 것으로 생각된다.

• Weed & Turfgrass Science
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• 제4권2호
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• pp.129-134
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• 2015

굼벵이는 각종 작물과 나무에 피해를 줄 뿐만 아니라 특히 골프장 잔디에 피해를 주는 주요해충의 하나이다. 본 연구는 18종의 식물정유(anise oil, camphor, castor, cinnamon, clove oil, citronella, fennel, geranium, lavender, lemongrass, linseed, neem, peppermint, pine, rosemary, tea-tree, thyme, turpentine)를 이용하여 굼벵이의 환경친화적 방제 가능성을 알아보고자 실내와 온실, 야외에서 실험을 수행하였다. Cap vial실험에서 홈줄풍뎅이 3령충에 효과가 높은 정유는 anise (88.9%)와 linseed oil (100%) 및 tea-tree oil (88.9%)이었다. 온실 pot에서 왜콩풍뎅이 3령충에 대한 anise와 linseed oil의 효과는 낮게 나타났다. 야외에서 anise와 linseed 및 tea-tree oil의 효과도 대상 굼벵이의 종류에 따라 차이를 보였다. 주둥무늬차색풍뎅이 번데기에 대해서는 tea-tree oil 처리에서만 32.6%의 보정사충율을 보였으나 등얼룩풍뎅이 3령충에 대해서는 anise와 linseed, tea-tree oil이 각각 54.8%, 51.6%, 56.5%의 보정사충율을 보였다.

• 한국패션뷰티학회지
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• 제3권3호
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• pp.31-40
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• 2005

The reason that aromatherapy began to draw attention again as the alternative therapy is because the essential oils that are used for aromatherapy not only allow the human body to maintain homeostasis but also have an effect on curing a mental feeling of fatigue and because these efficacies and effects are proceeding with being proved scientifically. Also, with coming to be high in a voice of concern about severally side effects that the modern medicine and the synthetic chemistry have, the aromatherapy came to be paid attention as the replacement therapy that can substitute for and supplement this. In particular, at the time of requiring a study on a substance to substitute for this after having been announced in Japan for 1998 a risk of Zincpyrithione that is being used as a component of dandruff treatment, it is thought that the aroma oil that was known for having an anti-dandruff effect has a merit of being easy in a user's access, thus it will be effective in curing the symptom of dandruff. Accordingly, this study conducted the following experiment in a bid to examine whether the aroma oil has the anti-microbial effect on the actual malassezia. First of all, it measured the minimum concentration of aroma oil in which the growth of malassezia is inhibited, by carrying out MIC(Minimum Inhibition Concentration) test in terms of anti-microbial activity against Malassezia furfur KCTC 7744 while using oils of Patchouli, Rosemary, Lavender, and Tea tree among aroma oils that are being used for dandruff treatment. As a result of that, Tea tree oil has the most excellent anti-microbial activity, and the measurement result of anti-microbial activity was shown in order of Lavender oil>Rosemary oil>Patchouli oil. Next, it carried out the is actually eased by applying Tea tree oil directly to the scalp of dandruff. In consequence of the experiment, the massage of Tea tree oil indicated the result of relieving the symptoms of dandruff such as scale, itch, pain, inflammation, and sebum, and among these symptoms of dandruff, it was shown to be most effective in easing itch. Especially, this clinical experiment was indicated that the massage of Tea tree oil has the most effect on relieving itch of targets with a dry-skin type who had no experience of dandruff treatment.

• International Journal of Internet, Broadcasting and Communication
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• 제14권3호
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• pp.171-181
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• 2022

Purpose: In this paper, we show our blending ratio of 10 types of Essential Oils that survives beneficial bacteria and kills harmful bacteria in the scalp, and we investigate the possibility of application of our blending ratio to the development of anti-dandruff products and the possibility of being used as a raw material for clinical beauty and customized cosmetics. Methods: The scalp microorganisms used in our study were M. furfur, S. epidermidis, E. coli, and P. nitroreducens. There are a total of 10 Essential Oils such as True Lavender, Lime, Roman chamomile, Rosemary camphor, Cedarwood, Geranium, Clove, Tea tree, Palmalosa, and Peppermint. The antibacterial test of the blended Essential Oil was carried out according to the test method of the standardized evaluation methodology of "Food and Food Additives Code". Since M. furfur is related to the growth of sebum in the scalp, in this study we used the fnLNB and the fnLNA with 20 ㎖ of whole fat cow milk added. Results: The blending ratio of EO, which inhibits dandruff-causing bacteria such as M. furfur, S. epidermidis, E. coli, and does not inhibit P. nitroreducens showing dominant growth in a healthy scalp, was B8(Clove 0.2%, Roman chamomile 0.5%, Tea tree 0.3%), B9(Geranium 0.1%, Palmarosa 0.1%, Roman chamomile 0.5%, Tea tree 0.3%), B10(Clove 0.1%, Geranium 0.1%, Palmarosa 0.1%, Roman chamomile 0.5%, Tea tree 0.2%). Conclusion: It is thought that the blending ratio of BEO obtained as a result of this study can provide a basis for use as an alternative to antibiotics in developing anti-dandruff drugs and emerge as a new alternative to solve scalp microbial imbalance. In order for EO to be used as a useful raw material for anti-dandruff preparation, researches on 1) Standardization (the effects of products differ according to the types, regions, climate, extraction methods, etc.), 2) Antimicrobial effects, 3) Safety, etc., must be established.

• 한국융합학회논문지
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• 제9권7호
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• pp.149-154
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• 2018

본 논문은 Lavender, Tea tree, Eucalyptus, Lemongrass 4종의 천연에센셜오일의 Streptpccous mutans 에 의한 치석형성억제 효과에 대하여 연구해 보고자 하였다. Streptpccous mutans 에 의한 치석형성 억제 활성 측정결과인 Lavender 에센셜 오일의 MAC (Minium anti-adhesive concentration, 최소부착저지 농도)가 1.0 % 이고 Tea tree 에센셜 오일, Eucaliptus 에센셜 오일, lemongrass 에센셜 오일의 MAC는 0.5 % 로 나타났다. 에센셜오일의 농도를 달리하여 Streptpccous mutans와 혼합한 배양액을 항온조에서 배양한 후 용액의 pH를 측정하여 최소부착 저지농도 이하에서 산의 생성으로 pH가 낮아진 것을 확인할 수 있었고, 이 결과는 에센셜 오일이 Streptpccous mutans 에 의한 산의 생성을 억제하는 효과가 있다는 것이다. 이러한 결과들로부터 천연에센셜오일 중 특히 Tea tree, Eucalyptus, Lemongrass는 치석생성을 억제하는 천연소재로 구강관리 제품개발에 활용할 수 있는 가능성을 확인할 수 있었다.

• 한국생활과학회지
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• 제17권5호
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• pp.1027-1035
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• 2008

Essentail oils and carrier oils are generally used for Aromatherapy. Therefore the toxicity, possibilities of irritations and sensitive reactions and injury of essential oils must be considered for clients and therapists. So that, in this studies a toxicity of jojoba and 4 species essential oils (fennel, mandarine, tea tree and cedarwood) were investigated by the measurement of MTT-assay and sirius red staining. Liver, kidney and brain tell were chosen for the cell viability assay and observation of morphological change. In the result, no cytotoxicity was observed on live., kidney and brain cell at concentration of 0.01 $\mu\el/m\el$ jojoba oil. And lysis and nucleus breaking were not observed at same concentration of jojoba oil on live., kidney and brain cell. fennel oil was showed 50% of cell viability and inhibited cell growth on liver, kidney and brain cell at relatively high concentration compared with the other oils. 50% of liver, kidney and brain cell viability and delayed cell growth of tea tree and mandarine oil were revealed at lower concentration than fennel oil. In cedarwood oil, 50% of liver cell viability at concentration of 0.00067 $\mu\el/m\el$ was showed, but cell viability and cell growth of kidney and beam cell were effected at the lowest concentration compared with other oils. So that, jojoba oil as using of carrier oil may be not harmful. And 3 essential oils from the fennel, tea tree and mamdarine may have very low toxicity, but cedarwood may be used carefully for inhalation. And over dosage of concentrated cedarwood oil should be not directly touched and exposured, and absolute essential oils must be diluted with carrier oils for topical and systematic massage.

• 한국응용곤충학회지
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• 제51권4호
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• pp.389-395
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• 2012

본 연구는 쌀을 비롯한 저장곡물을 가해하는 어리쌀바구미(Sitophilus zeamais) 성충에 대해서 8종의 식물정유의 훈증독성과 Y-tube를 이용한 후각반응으로 기피효과를 조사하였다. $10{\mu}{\ell}/L$ air의 농도에서 훈증독성시험 결과 pennyroyal과 tea tree 오일에서 각각 97%, 63%의 높은 살충효과를 보였다. 특히, pennyroyal oil($LT_{50}$, hour=3.87)은 tea tree oil($LT_{50}$, hour=4.09)보다 높고 빠른 살충력을 보였다. $10{\mu}{\ell}$/filter paper의 농도에서 후각계를 이용한 기피효과는 Cedarwood(80%), Cinnamon(83.3%), Cypress(81.1%), pennyroyal(97.1%) oil이 높은 기피활성을 보였으며, 이보다 낮은 $1{\mu}{\ell}$/filter paper의 농도에서도 Cinnamon(82.4%), Cypress(80.0%), pennyroyal(86.7%) 오일은 높은 기피활성을 나타냈다.