Korean Journal of Human Ecology (한국생활과학회지)

Korean Association of Human Ecology (한국생활과학회)
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A Cytotoxicity of Carrier Oil and Essential Oils on Cells by Using of Aromatherapy

향기요법에 사용하는 캐리어 오일과 에션셜 오일의 세포에 대한 독성

  • Yu, Byong-Soo (Div. of Bio-Nano Chemistry Natural Science College.Inst. of Advanced Cosmetic Materials Wonkwang University) ;
  • Kim, Sha-Sha (Div. of Bio-Nano Chemistry Natural Science College.Inst. of Advanced Cosmetic Materials Wonkwang University) ;
  • Yun, Young-Han (Div. of Bio-Nano Chemistry Natural Science College.Inst. of Advanced Cosmetic Materials Wonkwang University) ;
  • Kim, Ki-Young (Div. of Beauty Design Graduate School of Wonkwang University.Div. of Beauty Design Human Environmental College Wonkwang University)
  • 유병수 (원광대학교 자연과학대학 생명나노학부.고급미용재료연구소) ;
  • 김샤샤 (원광대학교 자연과학대학 생명나노학부.고급미용재료연구소) ;
  • 윤영한 (원광대학교 자연과학대학 생명나노학부.고급미용재료연구소) ;
  • 김기영 (원광대학교 대학원 뷰티디자인학과.생활과학대학 뷰티디자인학부)
  • Published : 2008.10.31
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Abstract

Essentail oils and carrier oils are generally used for Aromatherapy. Therefore the toxicity, possibilities of irritations and sensitive reactions and injury of essential oils must be considered for clients and therapists. So that, in this studies a toxicity of jojoba and 4 species essential oils (fennel, mandarine, tea tree and cedarwood) were investigated by the measurement of MTT-assay and sirius red staining. Liver, kidney and brain tell were chosen for the cell viability assay and observation of morphological change. In the result, no cytotoxicity was observed on live., kidney and brain cell at concentration of 0.01 $\mu\el/m\el$ jojoba oil. And lysis and nucleus breaking were not observed at same concentration of jojoba oil on live., kidney and brain cell. fennel oil was showed 50% of cell viability and inhibited cell growth on liver, kidney and brain cell at relatively high concentration compared with the other oils. 50% of liver, kidney and brain cell viability and delayed cell growth of tea tree and mandarine oil were revealed at lower concentration than fennel oil. In cedarwood oil, 50% of liver cell viability at concentration of 0.00067 $\mu\el/m\el$ was showed, but cell viability and cell growth of kidney and beam cell were effected at the lowest concentration compared with other oils. So that, jojoba oil as using of carrier oil may be not harmful. And 3 essential oils from the fennel, tea tree and mamdarine may have very low toxicity, but cedarwood may be used carefully for inhalation. And over dosage of concentrated cedarwood oil should be not directly touched and exposured, and absolute essential oils must be diluted with carrier oils for topical and systematic massage.

Keywords

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