• The Plant Pathology Journal
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• v.31 no.3
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• pp.278-289
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• 2015

Indigenous strains of Trichoderma species isolated from rhizosphere soils of Tea gardens of Assam, north eastern state of India were assessed for in vitro antagonism against two important tea fungal pathogens namely Pestalotia theae and Fusarium solani. A potent antagonist against both tea pathogenic fungi, designated as SDRLIN1, was selected and identified as Trichoderma viride. The strain also showed substantial antifungal activity against five standard phytopathogenic fungi. Culture filtrate collected from stationary growth phase of the antagonist demonstrated a significantly higher degree of inhibitory activity against all the test fungi, demonstrating the presence of an optimal blend of extracellular antifungal metabolites. Moreover, quantitative enzyme assay of exponential and stationary culture filtrates revealed that the activity of cellulase, ${\beta}$-1,3-glucanase, pectinase, and amylase was highest in the exponential phase, whereas the activity of proteases and chitinase was noted highest in the stationary phase. Morphological changes such as hyphal swelling and distortion were also observed in the fungal pathogen grown on potato dextrose agar containing stationary phase culture filtrate. Moreover, the antifungal activity of the filtrate was significantly reduced but not entirely after heat or proteinase K treatment, demonstrating substantial role of certain unknown thermostable antifungal compound(s) in the inhibitory activity.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.59-59
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• 2023

특허제도는 발명자에게 발명의 공개에 대한 대가로 일정기간 독점권을 인정하여 기술개발에 투자한 자금을 회수할 수 있는 기회를 제공하고 이렇게 공개된 발명은 새로운 기술개발을 위하여 누구나 이용할 수 있도록 하여 기술발전을 촉진할 뿐만아니라 이미 개발된 발명에 대한 중복투자나 중복연구를 하는 낭비를 방지하기 위한 목적을 가진다. 본 조사는 2022년 12월 31일까지의 정부 특허정보넷인 키프리스(www.kipris.or.kr)에서 꽃차 관련 상 표등록 특허 내용을 검색하여 정리하였으며 그결과는 다음과 같았다. 1. 2002년 연꽃차에 대한 것을 시작으로 최근 2022년 12월 31일까지 총 142개가 출원되었고 2022년에만 19개가 출원되었다. 2. 2022년 12월 31일까지 출원된 상표등록 특허 총 141개 중에서 포기 15개, 거절 27개, 등록 93개, 공고 7개였다. 3. 2022년 출원된 상표등록 특허 중 등록된 것은 19개였으며 개인이 출원한 것이 16개, 법인은 3개였다. 4. 출원된 내용중 상품분류는 01(농업용), 03(화장품), 28(놀이용품), 30(차류), 31(농산물), 32(음료), 33(알콜음료), 35(광고업), 40(재료처리업), 41(교육업), 43(음식료품 서비스업) 이었다.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2002.11a
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• pp.44.3-45
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• 2002

• Journal of the East Asian Society of Dietary Life
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• v.15 no.1
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• pp.57-64
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• 2005

The effects of water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower on serum lipid concentrations were evaluated in rats. Forty-eight male Sprague-Dawley rats weighing l00±l0 g were divided into six groups and fed high fat diets for six weeks. Experimental groups were administered with following diets; Control diet, animal, plant high fat diet and control and high fat diets with 2% water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower. Tissue weights of liver, lung, stomach, heart, kidney and spleen of high fat diet exposed rats were reduced by water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups. The concentrations of serum triglyceride in rats fed the water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower were lower than those in other groups. The concentrations of total cholesterol in water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower group were lower than those in high fat diet groups. The concentrations of HDL-cholesterol in serum of the water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups were significantly higher than those of other groups. The levels of LDL-cholesterol in serum of the water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups were tended to be lower than those of other groups. GPT and GOT activities were decreased in water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups and than in the high fat group. LDH activity was lower in the water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups than in the high fat group. These results suggest that water extracts of green tea scented with lotus Nelumbo nucifera Gaertner flower groups may reduce elevated levels of serum lipid concentrations in rats fed high fat diets.

• The Korean Journal of Community Living Science
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• v.21 no.1
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• pp.139-148
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• 2010

This study was conducted to restore our traditional Cheongtaejeon tea and to develop the special products. We inoculated Cheongtaejeon tea with lactobacilli (Lactobacillus plantarum CHO 25) and the mixed microbial strains (L. plantarum CHO 25 + Saccharomyces cerevisiae + Bacillus amyloliquefaciens CHO 104). We also examined the sensuous characteristics and physiological activity of Cheongtaejeon tea which was produced by the inoculation of microbial strains. The external appearance of Cheongtaejeon teas were not significant among the teas which were produced with or without the inoculation of L. plantarum CHO 25 and the mixed microbial strains. The taste of the tea increased most in Cheongtaejeon tea which was produced without the inoculation of microbial strains. The taste and liking of Cheongtaejeon tea which was inoculated with Aspergillus oryzae and Aspergillus niger decreased significantly, and it was not suitable to drink. Total phenolics compound contents, total flavonoid contents and DPPH ($\alpha,\alpha$-diphenyl-$\beta$-picryl-hydrazyl) radical scavenging activity of Cheongtaejeon tea extracts increased much more in the order of that produced with the inoculation of L. plantarum CHO 25, control and that with the mixed microbial strains. However, nitrite radical scavenging activity in 1,000 mg/L Cheongtaejeon tea hot water extracts were in the order of the control (94.4%), the inoculation of L. plantarum CHO 25 (93.6%) and the mixed microbial strains (91.1%). Overall results indicated that the sensuous characteristics increased most in Cheongtaejeon tea which was produced without the inoculation of microbial strains, and those physiological activities in tea with the inoculation of L. plantarum CHO 25.

• Journal of Bio-Environment Control
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• v.15 no.1
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• pp.107-113
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• 2006

These studies were carried out to investigate the quality of green tea processed by different pan-firing hand rolling and to select the best conditions during the procedure of green tea processing the results were as follows. At harvestion tea plant leaf, tea plants had 4 leaves. each leaf size was 7.5 cm'$\square$2.6 cm, and weight of 100 buds 46.6 g. The increase of pan-fired time decreased in the moisture content and yield of green tea. After processing green tea, 5 times pan-fired one had much more contents of total nitrogen (T-N:5.31%), caffeine (2.00%) and total amino acid (T.A.A. : 3,046 mg%) and less content of tannin (13.41%) than the other treatments. but 3 times pan-fired one had the highest contents of vitamin C (204.3 mg%) and chlorophyll (365.0 mg%) among three kinds pan-firing method. Sixteen kinds of free amino acid including theanine as a good taste component of pan-fired green tea were isolated and measured by HPLC. Free amino acid (FAA) contents were in the range of $2,683{\sim}2,925$ mg% and among various pan-firing methods, 5 tims pan-fired one had the highest content of F.A.A. Among these free amino acids, theanine occupied as much as $56.4{\sim}61.3%$ of F.A.A. Lightness, yellowness and redness of 5 times pan-fired green tea were middle value among three different treatments. Besides, it gained the highest score of sensory evaluation among them. These above results suggested that 5 times pan-fired green tea was considered to be the best level as pan-firing times of green tea processing.

• Natural Product Sciences
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• v.28 no.3
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• pp.121-129
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• 2022

Despite considerable efforts, cancer remains an aggressive killer worldwide. Chemotherapeutic drugs that are currently in use lead to destructive side effects and have not succeeded in fulfilling expectations. For centuries, medicinal plants are used for treating various diseases and are also known to have anticancer activity. The main aim of this research was to evaluate antiproliferative activity of saffron, clove, fenugreek, and green tea on Vero and MDA-MB-231 cell lines and to subsequently analyze the effect of these extracts on IRAK-4, TAK1, IKK-alpha, IKK-beta, NF-Kappa B, IRF3, IRF7 genes in Toll Like Receptors (TLRs) pathway. Antiproliferative assay was done by Neutral Red Dye uptake assay. Methanolic extract of green tea was found to be most effective against both cell lines as IC₅₀ was achieved at least concentration of the extract. For molecular studies, MDAMB-231 cells were sensitized with methanolic extract of green tea at same IC₅₀, and RT-PCR was performed to determine the relative expression of genes. Expression of IRAK-4, TAK1, IKK-beta, NF-Kappa B, IRF3 genes was down regulated and IRF7 and IKKalpha was upregulated. Green tea has a potential cytotoxic effect on both cell lines which was demonstrated by its effect on the expression of (TLRs) pathway genes.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.63-63
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• 2021

사회가 발달할수록 사람들의 건강과 웰빙에 대한 욕구는 더욱 늘어가고 이에 따라서 몸에 좋은 것들을 찾고 개발하여 다양한 음료와 차들이 판매되고 있는 실정이다. 이에 색과 기능성이 뛰어난 꽃차들에 대한 선호도가 점점 올라가고 있으며 그중에서도 색과 향과 맛 및 기능성까지 뛰어난 대표적인 꽃차가 백목련 꽃차이다. 본 연구에서는 노화억제 물질로 잘 알려져 있는 항산화물질들이 제조된 2017년부터 2021년까지의 연도에 따라서 차이가 있는 지를 알아보고자 수행하였으며 그 결과는 다음과 같았다. 1. Flavonoid 함량은 측정농도(1g/100ml)로 제조연도가 2017년 것은 499.0±11.0ppm, 2018년 것은 485.0±16.0ppm, 2019년 것은 422.0±13.0ppm, 2020년 것은 759.0±38.0ppm, 2021년 것은 509.0±14.0ppm으로 2020년에 제조한 것이 가장 높은 함량을 보였다. 2. Chlorogenic acid 함량은 측정농도(1g/100ml)로 제조연도가 2017년 것은 2062.0±63.0ppm, 2018년 것은 2253.0±21.0ppm, 2019년 것은 2035.0±25.0ppm, 2020년 것은 3363.0±52.0ppm, 2021년 것은 2359.0±35.0ppm의 함량을 보였다. 3. Catechin 함량은 측정농도(1g/100ml)로 제조연도가 2017년 것은 1343.0±42.0ppm, 2018년 것은 1470.0±14.0ppm, 2019년 것은 1325.0±17.0ppm, 2020년 것은 2210.0±35.0ppm, 2021년 것은 1541.0±24.0ppm의 함량을 보였다. 4. Tannic acid 함량은 측정농도(1g/100ml)로 제조연도가 2017년 것은 1403.0±42.0ppm, 2018년 것은 1530.0±14.0ppm, 2019년 것은 1385.0±17.0ppm, 2020년 것은 2270.0±35.0ppm, 2021년 것은 1601.0±24.0ppm의 함량을 보였다. 5. 모든 항산화물질에서 2020년에 제조한 꽃차가 가장 높은 함량을 보였다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.1
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• pp.58-61
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• 2003

Moisture content was 79.5%, banjhi shoot was 77.8% of the total and the weight was 29.4 g/100 buds in the harvested plant leaves used for manufacturing Okro tea. Okro tea heated at 75$^{\circ}C$ for 30 min showed the best quality among all the treatmants. Chemical components such as total nitrogen, theanine, arginine and chlorophyll were high in the tea treated at 75$^{\circ}C$ for 30 min compare with those of other treatments. The sensory evalution test In terms of appearance shape color and taste showed greater preferences in Okro tea treated at 75$^{\circ}C$ for 30 min than those of other treatments. The results suggested that heat treatment at 75$^{\circ}C$ for 30 min was the best condition for the final rolling process in manufacturing of Okro tea.

• Journal of Korean Society of Forest Science
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• v.75 no.1
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• pp.25-31
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• 1986

In order to contribute to the Korean tea-plant culture and tea industry by means of increasing the production of tea-plants, I have performed the tissue culture of the organs of the anther, leaf and stem. As for the culture-material, I have used the anther of tea (Thea sinensis) at the tetrad uninucleate microspore stage and used medium of modified Murashige and Skoog as the basal medium supplemented with the growth regulators of NAA and 2, 4-D, yeast, kinetin and others at various concentrations. As for the handling of material, I have followed the common methods of sterilization and microtoming and paraffine imbedding method and observed systematically periodic changes of the microspores in culture. I have divided the leaf, stem and root into segments and sterilized them and used the modified Murashige and Skoog as the basal medium and observed the differentiation of roots and callus and the results are as follows. 1. In case of anther, I have found 2n callus was found in 30 out of 100 segments in M2 medium. 2. The differentiation of roots appeared in 24.5% of total leaf segments cultured and in 50.5% of stem and in 43.9% of root. 3. When the differentiation of stem in different parts was observed, the most frequent differentiation was found in the second part of all the 4 parts. 4. The most frequent formation of callus was noticed from the anther-walls in case of anther culture and from the veins in case of leaf culture. It is concluded that the seedlings of tea-plant could be multiplied most by means of tissue culture of the second part of the tea-plant stem and reduction in the expenditures of tea-plant propagation was possible through tissue culture.