• 대한화장품학회지
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• 제37권1호
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• pp.67-73
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• 2011

치아의 세균들은 잇몸에서 염증반응을 일으켜서 치은염과 치주염같은 잇몸 질환의 원인이 된다. 따라서 잇몸질환의 예방과 치료를 위해서는 치아 세균에 의한 염증반응을 조절하는 것이 가장 효과적인 방법이다. 현재 대부분의 구강 관리 제품들은 살균제를 이용하여 구강 세균을 제거하는 방법을 사용하고 있다. 하지만 최근의 연구들은 심지어 열처리로 사멸된 구강 세균도 염증반응을 일으킬 수 있다는 사실을 보고하고 있다. 따라서 보다 효과적인 잇몸 염증반응억제를 위해서는 살균제를 이용한 방법에 대한 개선이 필요하다. 또한 아직까지 구강 세균에 의한 잇몸 염증반응의 기작과 효과적인 천연 염증 억제 물질들은 보고되어 있지 않다. 본 연구에서는 대표적인 치은염, 치주염 유발세균인 Prevotella intermedia와 인간의 잇몸상피세포를 이용하여, 실제로 잇몸에서 일어나는 염증반응의 기작을 연구하고, 이를 통해 효과적인 천연 잇몸 염증 완화 물질을 도출하려고 하였다. 실험 결과, Prevotella intermedia는 잇몸상피세포를 자극하여 염증매개인자인 IL-8을 분비하게 함으로써 잇몸 염증반응을 개시하였다. 또한 Prevotella intermedia에 의한 잇몸 염증반응은 기전적으로 COX-2, AP-1, TNF-${\alpha}$와 연관되어 있었으며, 녹차추출물은 Prevotella intermedia에 의한 잇몸 염증반응을 효과적으로 억제할 수 있음을 확인하였다. 따라서 본 연구는 치아 세균에 의한 잇몸 염증반응의 기전 연구를 통해서 효과적인 천연 잇몸질환 개선 물질을 도출했다는 점에서 중요한 의미를 가진다.

• 대한지역사회영양학회지
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• 제6권4호
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• pp.635-644
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• 2001

This study was carried out to investigate the intake patterns of health food according to the kind of disease of the elderly aged over 60 years living in the Chuncheon area. This study was conducted by using a questionnaire from December 1999 to the March 2000. The data was analyzed using a SPSS program. The results were summarized as follows : The poorer the self- perceived health condition, the lower the level of activity of daily living(ADL) and instrumental activities of daily living(IADL) were the higher. the frequency score of health food intake. The incidence of disease, such as arthritis(44.7%), hypertension(18.8%), digestive diseases(17.1%), diabetes(10.5%) were found to be higher. than other chronic diseases among the elderly. The frequency score of health food intake was not significantly different according to self-perceived health status, disease status, or the level of ADL and IADl.. The must important source of information for health food intake was the recommendation of friends and relatives(68.7%). Twenty eight point two percent of the elderly considered health foods to be effective, however 8.3% of the elderly considered them to be ineffective. The elderly with chronic diseases had taken health floods in the descending order of neuralgia-arthritis, hypertension, digestive disease, diabetes. The elderly with digestive diseases, diabetes, renal disease, neuralgia- arthritis and respiratory disease were inclined to habitually take health floods once or twice per year. Intake of Korean traditional folk food was as high as intake of registered health food and Chinese tonic medicine. The most frequently taken health food was tonic medicine, which is fo11owed by pumpkin, ginseng products, herbal tea formula with black goat, deer antler and Kye-so-ju. Intakes of health food such as tonic medicine, blood of the deer, royal jelly, aloe were found to be higher among the elderly with chronic diseases such as diabetes, hypertension, and digestive disease. However, intakes of ginseng products, deer antler, pumpkin, herbal tea formula with black goat and Kye-so-ju were found to be higher among the healthy elderly. The elderly with neuralgia-arthritis had taken sixteen kinds of health foods. From these results, a wide consumer education program should be developed to convince people of the importance of well-balanced diet and to choose proper health foods according to the elderly's health conditions. Also, comprehensive and scientific research into Korean traditional folk foods are needed for the correct use.

• 한국식품과학회지
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• 제36권6호
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• pp.979-985
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• 2004

본 연구에서는 Sprague Dawley 종 수컷 흰쥐에게 콜레스테롤이 1% 함유된 사료를 급여하여 고지혈증을 유발시키면서 녹차추출물, 메밀추출물, 포도잎추출물 추출물을 500 mg/kg B.W.의 농도로 4주간 매일 같은 시각에 경구투여하여 흰쥐의 혈액과 간장 내의 지질조성과 혈액응고기전 및 체내 지질과산화에 미치는 영향을 관찰하여 다음과 같은 결과를 얻었다. 전군에서 식이섭취량과 체중증가량 및 식이효율은 유의한 차이가 없어서 실험식이가 정상적인 성장에 영향을 미치지 않았다. 녹차추출물투여군은 고콜레스테롤 급여 대조군에 비해서 흰쥐의 꼬리에서의 출혈시간을 증가시켜서 항혈전효과가 있었다. 고콜레스테롤 급여로 인해 흰쥐에게 고지혈증이 유발되었으며 실험 시료를 경구투여하는데 따른 혈액중의 지질대사 개선효과는 없었다. 고콜레스테롤 급여로 흰쥐의 간장은 비대해지고 간장내 중성지방과 총콜레스테롤 함량도 현저히 증가하였으며 녹차추출물, 메밀추출물, 포도잎추출물은 간장 내 총콜레스테롤 함량을 감소시켜 간장 내 지질대사의 개선효과가 있었다. 고콜레스테롤의 급여는 혈장 내의 지질과산화물의 함량을 증가시키며 포도잎추출물은 체내 지질과산화를 억제시키는 효과가 있었으며 간에서는 메밀과 포도잎추출물이 지질과산화를 억제하였다.

• 대한지역사회영양학회지
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• 제19권1호
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• pp.84-94
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• 2014

Periodontal disease is one of the most common chronic inflammatory diseases in the oral cavity, and this is the leading cause of loss of teeth. Studies on the association between diet and periodontal diseases are very limited. The purpose of this study was to investigate the association between food and nutrient intakes and the prevalence of periodontitis. Subjects were 13,391 adults participating in the 2008-2010 Korea Health and Nutrition Examination Survey. Periodontitis was diagnosed by dentists using the Community Periodontal Index. Nutrient intakes were estimated by the 24-hour dietary recall. Consumption frequencies of foods were from the food frequency questionnaire. Subjects were categorized into 'smoking' or 'non-smoking' groups. Multiple logistic regression analysis was applied to determine the association between diet and periodontitis. The proportions of subjects having periodontitis were 26% in the non-smoking group and 37.5% in the smoking group. In the nonsmoking group, intakes of fruits, dairy products, green tea, energy and vitamin C were inversely associated with the prevalence of periodontitis, but fish and coffee intakes were positively associated with the prevalence of periodontitis after adjusting for covariates. In the smoking group, protein and retinol intakes were inversely associated with the prevalence of periodontitis after adjusting for covariates. These results suggest that certain food and nutrient intakes such as fruits, dairy products, green tea, vitamin C, protein, or retinol intakes may affect the prevalence of periodontitis among Korean adults. Further studies are required to confirm these findings in other research settings.

• Journal of Applied Biological Chemistry
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• 제64권3호
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• pp.237-244
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• 2021

Camellia sinensis, Green tea, contains phenolic compounds that act to scavenge reactive oxygen species (ROS), such as catechin, epicatechin, etc. In contrast with the tea leaf, the bioactivity of its fruit and the fruit peels remains still unclear. This study focused on the effects of fruit and fruit peels of C. sinensis (FC and PC) against oxidative DNA damage in NIH/3T3 cells. The scavenging effects of FC and PC on ROS were assessed using 1,1-diphenyl-2-picryl hydrazyl or 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radicals. The measurement of ROS in cellular levels was conducted by DCFDA reagent and the protein expression of γ-H2AX, H2AX, cleaved caspase-3, p53, and, p-p53 was analyzed by immunoblotting. The gene expressions of p53 and H2AX were assessed using polymerase chain reaction techniques. The major metabolites of FC and PC were quantitatively measured analyzed and the amounts of phenolic compounds and flavonoids in PC were greater than those in FC. Further, PC suppressed ROS production, which protects the oxidative stress-induced DNA damage through reducing H2AX, p53, and caspase-3 phosphorylation. These results refer that the protective effects of FC and PC are mediated by inhibition of p53 signaling pathways, probably via the bioactivity of phenolic compounds. Thus, FC and PC can serve as a potential antioxidant in DNA damage-associated diseases.

• Journal of Forest and Environmental Science
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• 제30권3호
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• pp.301-306
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• 2014

A study was carried out to identify the life cycle of Adoxophyes orana (Lepidoptera: Tortrididae) that inflicts tea tree leaves in Korea and selected three ecofriendly pesticides (Common name for commercial: Essential oil, Nemacatch, and Wormstop in Korean Farmers' Market) of A. orana for pest control. A. orana appeared to follow four life cycle phases a year; each presenting varying developmental periods dependent on seasonal and environmental factors. The fecundity of A. orana female was $24.6{\pm}4.10$ for $1^{st}$, $36.7{\pm}12.77$ for$ 2^{nd}$, and $27.9{\pm}4.22$ for $3^{rd}$ phase during 2011, while it was $65.0{\pm}32.72$, $49.7{\pm}30.27$, $63.8{\pm}27.22$ for corresponding phases during 2012. The average longevity of adult A. orana was 7.72 days. The average number of eggs deposited by each female in this study group was 44.62 with an average of 2.47. In three selected ecofriendly pesticides, the mortality of A. orana on treating with the Essential oil [The essential oil of Chamaecyparis obtuse (100%)] and Nemacatch [Azadirachtin 800-900 ppm (75%)] were 36.67% and 43.33% after 3 days and were 48.30% and 56.67% after 7days, respectively. Besides, the mortality of A. orana on treating with Wormstop [Azadirachtin 500 ppm (5%) and Salannin+Liminoids (95%)] was 61.67% and 78.33% after 3 and 7 days, respectively. Therefore, the application of Wormstop was the most useful to control the diseases caused by A. orana.

• Asian Pacific Journal of Cancer Prevention
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• 제16권10호
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• pp.4199-4202
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• 2015

Background: Hepatitis B virus infection is one of the major world health problems. Epigallocatechin-3 gallate is the major component of the polyphenolic fraction of green tea and it has an anti-viral, anti-mutagenic, anti-tumorigenic, anti-angiogenic, anti-proliferative, and/or pro-apoptotic effects on mammalian cells. In this study, our aim was to investigate the inhibition of HBV replication by epigallocatechin-3 gallate in the Hep3B2.1-7 hepatocellular carcinoma cell line. Materials and Methods: HBV-replicating Hep3B2.1-7 cells were used to investigate the preventive effects of epigallocatechin-3 gallate on HBV DNA replication. The expression levels of HBsAg and HBeAg were determined using ELISA. Quantitative real-time-PCR was applied for the determination of the expression level of HBV DNA. Results: Cytotoxicity of epigallocathechin-3-gallate was not observed in the hepatic carcinoma cell line when the dose was lower than $100{\mu}M$. The ELISA method demonstrated that epigallocatechin-3 gallate have strong effects on HBsAg and HBeAg levels. Also it was detected by real-time PCR that epigallocatechin-3 gallate could prevent HBV DNA replication. Conclusions: The obtained data pointed out that although the exact mechanism of HBV DNA replication and related diseases remains unclear, epigallocatechin-3 gallate has a potential as an effective anti-HBV agent with low toxicity.

• 동아시아식생활학회지
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• 제18권1호
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• pp.104-110
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• 2008

This research was designed and implemented to evaluate how the composition of yaksuncha can affect the health of individuals suffering from diet-related diseases such as obesity and hyperlipidemia by prescribing Yaksun as a nutritional supplement with daily meals. The yaksunucha was prepared as Koekac, Sansa, Heshouwu and Woolong tea and the tea's effects on serum lipids and oxidative stress were evaluated by clinical procedures. The yaksuncha significantly increased HDL-cholesterol and decrease of LDL-cholesterol concentrations in serum. It also had significant effects on decreasing oxidative stress and homocystein levels. The physical characteristics of the yaksunucha were also examined, showing. Brix, pH and titratable acidity values of 1.4, 5.50 and 0.05% respectively. It is thought that scientific and objective evaluations were completed on the components of the yaksuncha prescription. Thus one can concluded that the components could be applied not only in the form of tea, but also various food. The data derived from this study provides basic information that will aid in the application of oriental medicinal resources to other foods as well as facilitate the study of medicinal herbs within the field of functional food research, which already draws sizable attention worldwide.

• 농업과학연구
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• 제47권4호
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• pp.1071-1081
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• 2020

Essential oils (EOs) have been shown to be plant-extracted antimicrobial agents. However, there are limited studies investigating the efficacy of EOs against pathogens. Among them, tea tree oil (TTO) is extracted from Melaleuca alternifolia, which is also used as an antifungal agent. In this study, the effect of TTO was investigated on the suppression of melon powdery mildew caused by Podosphaera xanthii and tomato leaf mold disease caused by Passalora fulva. Both powdery mildew and leaf mold diseases were significantly suppressed by a spray of TTO. Eighty percent of powdery mildew and 81% of leaf mold disease of the control value were suppressed by 0.5% TTO liquid, when sprayed 3 times every 7 days on the melon and tomato leaves. Inhibition of mycelial growth was also greatly affected by different concentrations of TTO against four different fungal pathogens. Ninety-eight percent of Pseudocercospora fuligena, 97% of P. fulva, 95% of Botrytis cinerea, and 94% of Phytophthora infestans mycelial growth were inhibited by 0.2% to 1.0% of TTO contained in plate media, respectively. However, phytotoxicity in plants by the TTO treatments was revealed when melon and tomato leaves were sprayed with a 1% and 2% concentration of TTO, respectively. Therefore, our findings show that TTO has high antifungal effects against various plant pathogens that occur during crop cultivation. We also suggest that when applying TTO to plant leaves, it is necessary to establish an accurate treatment concentration for different crops.

• 한국식품저장유통학회지
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• 제23권2호
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• pp.188-193
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• 2016

홍차 분말의 첨가량을 0~8%로 달리하여 제조한 스펀지 케이크의 물리 화학적 품질, 항산화 활성 및 소비자 기호도를 비교하였다. 케이크의 pH와 높이는 홍차 분말의 첨가량이 증가할수록 유의적으로 감소하였으며(p<0.05), 굽기 손실률의 경우 홍차 분말의 함량이 증가함에 따라 유의적으로 증가하였다(p<0.05). 경도는 0~4% 시료 간에서는 유의적인 차이는 없었다(p>0.05). 한편 명도($L^*$)는 붉은색을 띄는 홍차 분말 고유색의 영향으로 첨가량이 증가할수록 유의적으로 감소하였고(p<0.05), 적색도($a^*$)와 유의적으로 증가하였다. 황색도($b^*$)의 경우 증가하는 경향을 보였으나, 0~4%, 6~8% 시료 간에 유의적인 차이는 발견되지 않았다(p>0.05). 항산화 활성을 나타내는 전자공여능과 ABTS 라디칼 소거능 활성은 홍차분말을 첨가할수록 유의적으로 증가하는 경향이 나타났다(p<0.05). 소비자 기호도 결과 2~4% 첨가군이 색과 부드러움을 제외한 나머지 항목에서 대조군보다 높게 평가되었으며, 따라서 관능품질과 건강기능성 효과 등을 고려할 때 소비자의 기호를 충족시키기 위한 최적 첨가비율은 2~4%가 가장 적합한 것으로 판단된다.