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The Factors for the Formation of Carcinogenic N-Nitrosamine from Dried Marine Food Products (수산 건제품중 발암성 N-NITROSAMINE의 생성 요인)

  • SUNG Nak-Ju;KANG Shin-Kwon;LEE Soo-Jung;KIM Sung-Hee
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.3
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    • pp.247-258
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    • 1994
  • A total of 31 commercial dried marine food products, consisting of 14 fishes, 2 shellfishes and 2 seaweeds species were analyzed for their contents of precusors of N-nitrosamine such as dimethylamine(DMA), trimethylamine(TMA), trimethylamine oxide (TMAO), betaine and nitrate and nitrite nitrogen as factors of N-nitrosamine formation. Carcinogenic N-nitrosamines were extracted by a steam distillation apparatus and were analyzed for their components using a gas chromatography-thermal energy analyzer. N-nitrosodimethylamine(NDMA) was confirmed by a gas chromatography-mass spectrometry. The contents of betaine nitrogen in samples were in the range of $5.2{\sim}373.8mg\%$ and were significantly higher than tertiary amines such as TMA and TMAO. DMA nitrogen in those samples was in the range of trace-31.2ppm and was high, in the dried shark(31.2ppm), alaska pollack($22.9{\sim}24.3ppm$) and octopus($17.9{\sim}18.4ppm$). In dried laver and sea mustard, however, amines were not detected at all. The levels of nitrate nitrogen in the dried marine samples ranged from zero to 16.8ppm and were high in the dried stingray(16.8ppm), alaska pollack(16.3ppm) and squid($2.2{\sim}12.4ppm$), but were less than 1.0 ppm in other samples. The levels of nitrite nitrogen were lower than those of nitrate nitrogen and it was not detected in dried sea cucumber, laver and sea mustard. Twenty eight of 31 samples contained NDMA($range=1.2{\sim}86.0ppb$), which was the only volatile N-nitroso compound found. The NDMA levels of dried stingray($2.8{\sim}86.0ppb$), alaska pollack($8.2{\sim}55.5ppb$), squid($3.3{\sim}53.2ppb$), yellow corvenia($45.9ppb$) and plain dried shrimp($15.4{\sim}17.9ppb$) were high. However, it was not detected in dried sea cucumber, laver and sea mustard. Samples, containing high levels of NDMA, also contained high nitrate and nitrite nitrogen. From above results, it can be concluded that nitrate and nitrite were major factors for the formation of NDMA in dried marine food products.

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Analysis of Optical Characteristic Near the Cloud Base of Before Precipitation Over the Yeongdong Region in Winter (영동지역 겨울철 스캔라이다로 관측된 강수 이전 운저 인근 수상체의 광학 특성 분석)

  • Nam, Hyoung-Gu;Kim, Yoo-Jun;Kim, Seon-Jeong;Lee, Jin-Hwa;Kim, Geon-Tea;An, Bo-Yeong;Shim, Jae-Kwan;Jeon, Gye-hak;Choi, Byoung-Choel;Kim, Byung-Gon
    • Korean Journal of Remote Sensing
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    • v.34 no.2_1
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    • pp.237-248
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    • 2018
  • The vertical distribution of hydrometeor before precipitation near the cloud base has been analyzed using a scanning lidar, rawinsonde data, and Cloud-Resolving Storm Simulator (CReSS). This study mostly focuses on 13 Desember 2016 only. The typical synoptic pattern of lake-effect snowstorm induced easterly in the Yeongdong region. Clouds generated due to high temperature difference between 850 hPa and sea surface (SST) penentrated in the Yeongdong region along with northerly and northeasterly, which eventually resulted precipitation. The cloud base height before the precipitation changed from 750 m to 1,280 m, which was in agreement with that from ceilometer at Sokcho. However, ceilometer tended to detect the cloud base 50 m ~ 100 m below strong signal of lidar backscattering coefficient. As a result, the depolarization ratio increased vertically while the backscattering coefficient decreased about 1,010 m~1,200 m above the ground. Lidar signal might be interpreted to be attenuated with the penetration depth of the cloud layer with of nonspherical hydrometeor (snow, ice cloud). An increase in backscattering signal and a decrease in depolarization ratio occured in the layer of 800 to 1,010 m, probably being associated with an increase in non-spherical particles. There seemed to be a shallow liquid layer with a low depolarization ratio (<0.1) in the layer of 850~900 m. As the altitude increases in the 680 m~850 m, the backscattering coefficient and depolarization ratio increase at the same time. In this range of height, the maximum value (0.6) is displayed. Such a result can be inferred that the nonspherical hydrometeor are distributed by a low density. At this time, the depolarization ratio and the backscattering coefficient did not increase under observed melting layer of 680 m. The lidar has a disadvantage that it is difficult for its beam to penetrate deep into clouds due to attenuation problem. However it is promising to distinguish hydrometeor morphology by utilizing the depolarization ratio and the backscattering coefficient, since its vertical high resolution (2.5 m) enable us to analyze detailed cloud microphysics. It would contribute to understanding cloud microphysics of cold clouds and snowfall when remote sensings including lidar, radar, and in-situ measurements could be timely utilized altogether.

Effect of Germanium Foliar Spray Application on Growth Characteristics and Germanium Absorption in Rice (게르마늄 엽면살포가 벼의 생육과 게르마늄 흡수에 미치는 영향)

  • Park, Jong-Hwan;Seo, Dong-Cheol;Kim, Seong-Heon;Lee, Choong-Heon;Lee, Seong-Tea;Choi, Jeong-Ho;Kim, Hong-Chul;Ha, Yeong-Rae;Cho, Ju-Sik;Heo, Jong-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.4
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    • pp.649-656
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    • 2012
  • To obtain the basic information for agricultural utilization of germanium (Ge), the growth characteristics and Ge absorption in rice plant were investigated under different Ge concentrations by foliar spray application. The Ge concentrations were treated with 0 (control), 10, 20, 40 and $80mg\;L^{-1}$ in pot (1 5000$^{-1}$ a), respectively. The Ge absorption rate in rice by foliar spray application with $80mg\;L^{-1}$ in pot was higher in the order of leaf (5.75%) > stem (4.52%) > root (<0.01%). By foliar spray application, the Ge content in rice was higher in the order of $80mg\;L^{-1}$ > $40mg\;L^{-1}$ > $20mg\;L^{-1}$ > $10mg\;L^{-1}$. When rice was treated with $80mg\;L^{-1}$ of Ge, the Ge content in rice grain was higher in the order of rice bran ($0.21mg\;pot^{-1}$) $\gg$ brown rice ($0.04mg\;pot^{-1}$) ${\geq}$ polished rice ($0.03mg\;pot^{-1}$). By foliar spray application, the Ge uptake in rice bran was higher than that in other parts. Therefore, optimum Ge concentration by foliar spray application was $80mg\;L^{-1}$ in pot based on the results from the Ge treatments.

Mitigation Effects of Foliar-Applied Hydrogen Peroxide on Drought Stress in Sorghum bicolor (과산화수소 엽면 처리에 의한 수수에서 한발 스트레스 완화 효과)

  • Shim, Doo-Do;Lee, Seung-Ha;Chung, Jong-Il;Kim, Min Chul;Chung, Jung-Sung;Lee, Yeong-Hun;Jeon, Seung-Ho;Song, Gi-Eun;Shim, Sang-In
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.2
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    • pp.113-123
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    • 2020
  • Global climatic change and increasing climatic instability threaten crop productivity. Due to climatic change, drought stress is occurring more frequently in crop fields. In this study, we investigated the effect of treatment with hydrogen peroxide (H2O2) before leaf development on the growth and yield of sorghum for minimizing the damage of crops to drought. To assess the effect of H2O2 on the growth of sorghum plant, 10 mM H2O2 was used to treat sorghum leaves at the 3-leaf stage during growth in field conditions. Plant height, stem diameter, leaf length, and leaf width were increased by 7.6%, 9.6%, 8.3% and 11.5%, respectively. SPAD value, chlorophyll fluorescence (Fv/Fm), photosynthetic rate, stomatal conductance, and transpiration rate were increased by 3.0%, 4.9%, 26.0%, 23.4% and 12.7%, respectively. The amount of H2O2 in the leaf tissue of sorghum plant treated with 10 mM H2O2 was 0.7% of the applied amount after 1 hour. The level increased to approximately 1.0% after 6 hours. The highest antioxidant activity measured by the Oxygen Radical Absorbance Capacity assay was 847.3 µmol·g-1 at 6 hour after treatment. However, in the well-watered condition, the concentration of H2O2 in the plant treated by the foliar application of H2O2 was 227.8 µmol·g-1 higher than that of the untreated control. H2O2 treatment improved all the yield components and yield-related factors. Panicle length, plant dry weight, panicle weight, seed weight per plant, seed weight per unit area, and thousand seed weight were increased by 8.8%, 18.0%, 24.4%, 24.7%, 29.9% and 7.1%, respectively. Proteomic analysis showed that H2O2 treatment in sorghum increased the tolerance to drought stress and maintained growth and yield by ameliorating oxidative stress.

Composition and Cell Cytotoxicity of Essential Oil from Caryopteris incana Miq. in Korea (층꽃나무(Caryopteris incana Miq) 정유의 성분 분석과 세포 독성 평가)

  • Kim, Song-Mun
    • Applied Biological Chemistry
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    • v.51 no.3
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    • pp.238-244
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    • 2008
  • The essential oil was obtained from the aerial part of Caryopteris incana Miq. by steam distillation, samples were collected by headspace (HS) and solid-phase microextraction (SPME) methods, and the compositions of the essential oil were analyzed by gas chromatography-mass spectrometry (GCMS). The fragrance of the essential oil was fougere and woody. There were sixty-nine constituents in the essential oil: 28 carbohydrates, 22 alcohols, 7 acetates, 7 ketones, 3 aldehydes, and 2 others. Major constituents were 4,6,6-trimethyl [1S-($1{\alpha},2{\beta},5{\alpha}$)]-bicyclo[3.1.1]hept-3-en-2-ol (11.8%), taucadinol (9.4%), myrtenyl acetate (9.2%), pinocarvone (7.0%), 1-hydroxy-1,7-dimethyl-4-isopropyl-2,7-cyclodecadiene (6.3%), ${\delta}$-3-carene (6.2%). By SPME extraction, forty-nine constituents were identified: 22 hydrocarbons, 16 alcohols, 6 acetates, 3 ketones, and 2 ethers. Major constituents of the SPME-extracted sample were ${\delta}$-3-carene (12.6%), (-)-myrtenyl acetate (11.2%), 6,6-dimethyl-2-methylene-bicycol [3.1.1] heptan-3-o1 (10.9%), pinocarvone (9.3%). By HS extraction, ten constituents were identified: 5 hydrocarbons, 2 amines, 1 alcohol, and 2 others. Major constituents of the HS-extracted sample were (Z)-2-fluoro-2-butene (34.9%), ${\delta}$-3-carene (6.9%), 6-(4-chlorophenul)tetrahydro-2-methyl-2H-1,2-oxazine (5.9%). The $IC_{50}$ value (0.011 ${\mu}g/mg$) in MTT assay using HaCaT keratinocyte cell line was lower than those of commercially-selling rosemary and tea tree, suggesting more toxicological studies are needed for commercial use of the essential oil of Caryopteris incana Miq.

Establishment of an Optimal Washing Condition of a High Temperature Steaming System for the Production of High Quality Pork (고품질 돈육 생산을 위한 고온증기세척수의 최적 조건)

  • Yang, H.S.;Jeong, J.Y.;Moon, S.H.;Park, G.B.;Joo, Seon-Tea
    • Journal of Animal Science and Technology
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    • v.49 no.1
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    • pp.121-128
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    • 2007
  • The objective of this study was to determine the efficacy of different water temperatures and time of spray-washing on the removal of bacteria contamination from surface of pork carcass and to obtain better meat quality with high temperature stream between 60 to 90℃ for the time ranged from 10 to 60 sec. Results showed that total plate counts were significantly decreased with increasing steam temperature(P<0.05). Similar results were found with the spray time(P<0.05). The lightness(CIE L*) value was significantly increased in both loin and ham cuts over 80℃(P<0.05). Ultimate pH of muscle and water-holding capacity were decreased with increasing steam temperature(P<0.05). There was a significant difference in solubility of sarcoplasmic protein between 70℃ and 80℃ of the steam treatments with higher value at 70℃(P<0.05). Again, longer than 30 sec spray at 70℃ steam, the lightness value of pork was significantly increased(P<0.05), while pH and water-holding capacity of muscle were significantly decreased(P<0.05). Therefore, a desirable pork quality would be achieved with spray-steam washing at 70℃ for 20 sec on pork carcass.

A Study on the Characteristics of Water Pollution in Rural Areas (농촌유역(農村流域)에서의 수질오염(水質汚染) 특성(特性)에 관한 연구(硏究))

  • Kim, Han-Tea;Kwun, Soon-Kuk
    • Korean Journal of Environmental Agriculture
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    • v.12 no.2
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    • pp.129-143
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    • 1993
  • The objective of this study is to understand the status of the water pollution in rural areas and to furnish a basic material for the management of the water pollution in rural areas. For this purpose, the Bokha river basin, Ichon-Gun, Kyungki-Do considering as a typical agricultural area was selected as a representative experimental watershed. The characteristics of water pollution in streams of the Bokha river basin was revealed by investigating and analyzing data collected for the source of pollution, water qualities in reaches of the stream, the degree of contribution to the river contamination by pollution mass produced from each source, and the status of the self-purification at the main stream. The most important source of the water pollution in investigated watershed was livestock, and the next important one were in the order of population, land use, and industry. The water quality of the Bokha river was relatively favorable judging from the BOD and COD concentration, however since the concentration of T-N and T-P showed significantly large values, it was concluded that the river was seriously contaminated by the nutrient material. The main cause of the river contamination was proved due to livestock waste. For the T-N, both land use and livestock were much more contributied to the pollution than any other source, which characterized the typical water pollution of rural areas. Run-off ratios for the Bokha river tributaries to the main stream were changed according to the similar trend to the variation of discharges in the branch streams. For the value of the self-purification constant at the main stream, it showed smaller value in the downstream reach than the middle-stream and upstream reaches, where could possibly have smaller reoxidation action due to slower velocity and deeper water depth.

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Comparison of the Content of Shizandrin, Gomisin A and Gomisin N in Schisandra Fruit by Water Extraction Condition (오미자의 물 추출조건에 따른 shizandrin, gomisin A, gomisin N의 함량 비교)

  • Kim, Hee-Soon;Moon, Hyun-Kyung;Lee, Young-Ju;Lee, Chun-Young;Hwang, Kwang-Ho;Kim, Ouk-Hee;Yoo, In-Sil;Jung, Kweon
    • Journal of Food Hygiene and Safety
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    • v.30 no.1
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    • pp.59-64
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    • 2015
  • This study compared the content change of physiological active substances according to the extraction condition when we generally drink the extractive of Schisandra fruit with using water. Physiological active substances of the Schisandra fruit, schizandrin, gomisin A and gomisin N were analysed with HPLC. The results of extracting 1 dose of the Schisandra fruit tea, which is some as 1 g of Schisandra fruit, in 100 mL of water by differing the extraction type, extraction temperature and extraction time were of the followings. The total content of schizandrin, gomisin A and gomisin N of powder type in $26^{\circ}C$ leaching was the highest (p < 0.05) as the leaching content of 24 hours (5.54 mg/g) showed difference with different times and the contents of raw type Schisandra fruit were less than 0.1 mg/g in all of extraction time. The contents of powder type Schisandra fruit in $60^{\circ}C$ heating extraction was over 4.50 mg/g except for the 5 minute (2.94 mg/g), and the contents of raw Schisandra fruit were less than 0.3 mg/g. In case of $100^{\circ}C$ heating extraction, the contents of powder type Schisandra fruit showed over 5.10 mg/g in every time zone, and the raw type Schisandra fruit was less than 1.0 mg/g.

A Study of Burcucumber Biochars to Remediate Soil Pb Considering GWP (Global Warming Potential) (GWP (Global Warming Potential)를 고려한 가시박 바이오차르의 토양 납 제거 효과 분석)

  • Kim, You Jin;Park, Han;Kim, Min-Ho;Seo, Sung Hee;Ok, Yong Sik;Yoo, Gayoung
    • Journal of Korean Society of Environmental Engineers
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    • v.37 no.7
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    • pp.432-440
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    • 2015
  • Biochar, a by-product from pyrolysis of biomass, is a promising option to mitigate climate change by increasing soil carbon sequestration. This material is also considered to have potential to remediate a soil with heavy metal pollution by increasing the soil's adsorptive capacity. This study conducted the assessment of two biochars considering the climate change mitigation potential and heavy metal removal capacity at the same time. Two kinds of biochars (BC_Ch, TW_Ch) were prepared by pyrolyzing the biomass of burcucumber (BC_Bm) and tea waste (TW_Bm). The soils polluted with Pb were mixed with biochars or biomass and incubated for 60 d. During the incubation, $CO_2$, $CH_4$, and $N_2O$ were regularly measured and the soil before and after incubation was analyzed for chemical and biological parameters including the acetate extractable Pb. The results showed that only the BC_Ch treatment significantly reduced the amount of Pb after 60 d incubation. During the incubation, the $CO_2$ and $N_2O$ emissions from the BC_Ch and TW_Ch were decreased by 24% and 34% compared to the BC_Bm and TW_Bm, respectively. The $CH_4$ emissions were not significantly affected by biochar treatments. We calculated the GWP considering the production of amendment materials, application to the soils, removal of Pb, and soil carbon storage. The BC_Ch treatment had the most negative value because it had the higher Pb adsorption and soil carbon sequestration. Our results imply that if we apply biochar made from burcucumber, we could expect the pollution reduction and climate change mitigation at the same time.

Incidence of Rice stripe virus during 2002 to 2004 in Korea and Chemical Control of Small Brown Plant Hopper (2002-2004년의 벼줄무늬잎마름병 발생상황 및 약제처리에 의한 애멸구의 화학적 방제)

  • Park, Jin-Woo;Jin, Tae-Seong;Choi, Hong-Soo;Lee, Su-Heon;Shin, Dong-Bum;Oh, In-Seok;Lee, Sang-Guei;Lee, Min-Ho;Choi, Byeong-Ryeol;Bae, Soon-Do;Kim, Jin-Young;Han, Kwang-Seop;Noh, Tae-Hwan;Ko, Sug-Ju;Park, Jong-Dae;Lee, Bong-Choon;Kim, Tae-Sung;Chung, Bu-Keun;Hong, Sung-Jun;Kim, Choong-Hoe;Park, Hyung-Man;Lee, Key-Woon
    • The Korean Journal of Pesticide Science
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    • v.13 no.4
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    • pp.309-314
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    • 2009
  • Incidence of rice stripe disease, caused by Rice stripe tenuivirus (RSV), was surveyed during 2002 to 2004. The incidence area and ratio of diseased fields were decreased gradually during those period. Rate of diseased plants were 45.8%, 45.0% and 43.7% in the susceptible cultivars Chucheong, Saechucheong and Ilpum, respectively. However, the rate was 4.4% in resistant cultivar Hwaseong. In addition, breakdown rate was also significantly high in the susceptible cultivars in Chucheong, Saechucheong and Ilpum showing 33.6, 33.2 and 31.9%, respectively. In Hwaseong, the breakdown rate was 0.8%. Collection efficiency was compared between two insect vector collection methods. Insect-sucking machine method was much more efficient than sweeping net method in collecting small brown plant hopper (SBPH). According to the survey of the population density of the insect vector during 2002 to 2004, the national average population density was gradually decreased year by year, 3.6, 2.3, and 1.3%, respectively. This result was significantly related with the decrease of the incidence of rice stripe disease. Control efficiency of rice stripe disease by treating several seedling box and water surface with insecticides against SBPH resulted that imidacloprid GR, Fipronil FG, Clothianidin+Probenazole GR and Thiamethoxam GR showed over 80% of control efficiency at 28 days after treatment at the early stage in nursery.