• 제목/요약/키워드: Taxonomic study

검색결과 946건 처리시간 0.031초

DNA-DNA Hybridization에 의한 Bacillus coagulans의 분류학적 연구 (Taxonomic Study of Bacillus coagulans by Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization Technique)

  • Chung, Chi-Kwan
    • 한국미생물·생명공학회지
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    • 제4권4호
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    • pp.166-178
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    • 1976
  • 서로 다른 11주의 Bacillus coagulans와 13종의 Bacillus 속 14주를 deoxyribonucleic acid(DNA)-DNA hybridization method에 의해서 분류학적인 연구를 하였다. 사용한 B. coagulans 11주중 6주는 흙에서(일본 오사까교외) 분리했고, 나머지 5주는 ATCC, IFO에서 authentic strains을 얻어서 사용했다. 사용된 B. coagulans는 Bergey's Manual(8th ed)에 의거 Gordon씨들의 방법으로 동정한 결과 B. coagulans로서 확인되었다. 이렇게 동정된 B. coagulans을 분자 생물학적 차원에서 지금까지의 Conventional taxonomic study와의 관계를 연구하기 위해서 사용한 11주의 B. coagulans중 ATCC 7070을 $^3$H labeled input 즉 standard로 해서 사용했을 때 B. coagulans 내의 intraspecific DNA homology indexes는 76% 이상으로 나타났다. 이와같은 발견은 Bergey's Manual에 의거한 conventional taxonomic study의 결과와 잘 일치하고 있었음으로 새로 분리한 6주와 authentic sources로부터 받은 5주는 같은 group의 B. coagulans라는 사실을 입증해 주었다. 그리고 B. coagulans와 다른 species의 Bacillus 속 즉 B. pumilus(168), B. licheniformis (IFO 12107), B. pumilus(IFO12110), B. firmus(ATCC 14575), B. lentus(ATCC 10840), B. circulans(ATCC 4513), B.macelans(ATCC 8244), B.polymyxa, (ATCC 842), B.sphaericus(ATCC 14577), B.brevis(ATCC 8246, IFO 12334), B.laterosporus(ATCC 64), B. pantothenticus(ATCC 14576) interspecific DNA homology indexes가 각각 2~4%을 보임으로써 B. coagulans는 molecular level면에서 이들 Bacillus 속과는 상동성 관계가 적음을 나타내었다. 반면에 B. coagulans(ATCC 7050)와 E. coli(F-12)와의 상동성은 1%이하였다.

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문제중심학습(PBL)의 실행에 관한 수학교사의 인식 조사 : 카드 활용 면담을 이용한 분류체계 및 성분 분석을 중심으로 (On Mathematics Teachers' Recognition about Problem-Based Learning(PBL) Implementation : Taxonomic Analysis & Componential Analysis by using cards)

  • 허난;하영화
    • 한국학교수학회논문집
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    • 제14권2호
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    • pp.143-161
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    • 2011
  • 수학교과에서 문제중심학습(PBL)은 학습자가 수학적 지식을 활용하여 문제를 해결해 나가는 과정 중에 수학적 개념과 원리를 알게 되고 수학적 사고 능력을 시켜줄 수 있는 교수 학습 방법으로 최근 관심이 높아지고 있다. 그러나 이러한 관심에 비해 실제 적용은 미미한 실정이다. 따라서 본 연구에서는 PBL의 학교 현장 적용을 위하여 수학교사들이 실제적으로 느끼는 어려움과 요구를 구체적으로 파악하고자 2명의 수학 교사를 대상으로 하여 카드활용 심층면담을 이용한 분류체계 분석과 성분 분석을 실시하였다. 그 결과 성공적인 PBL 적용을 위해서는 수학과 PBL 문제 개발에 대한 구체적인 방법의 안내와 수학교과에 적합한 PBL 학습 과정 안내 등의 구체적인 실행 방법에 대한 요구가 있음을 알 수 있었다.

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Data-processing pipeline and database design for integrated analysis of mycoviruses

  • Je, Mikyung;Son, Hyeon Seok;Kim, Hayeon
    • International journal of advanced smart convergence
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    • 제8권3호
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    • pp.115-122
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    • 2019
  • Recent and ongoing discoveries of mycoviruses with new properties demand the development of an appropriate research infrastructure to analyze their evolution and classification. In particular, the discovery of negative-sense single-stranded mycoviruses is worth noting in genome types in which double-stranded RNA virus and positive-sense single-stranded RNA virus were predominant. In addition, some genomic properties of mycoviruses are more interesting because they have been reported to have similarities with the pathogenic virus family that infects humans and animals. Genetic information on mycoviruses continues to accumulate in public repositories; however, these databases have some difficulty reflecting the latest taxonomic information and obtaining specialized data for mycoviruses. Therefore, in this study, we developed a bioinformatics-based pipeline to efficiently utilize this genetic information. We also designed a schema for data processing and database construction and an algorithm to keep taxonomic information of mycoviruses up to date. The pipeline and database (termed 'mycoVDB') presented in this study are expected to serve as useful foundations for improving the accuracy and efficiency of future research on mycoviruses.

Assessment of anatomical characteristics of the medicinal plant African cherry (Prunus africana) for its accurate taxonomic identification

  • Komakech, Richard;Yang, Sungyu;Song, Jun Ho;Choi, Goya;Kim, Yong-Goo;Okello, Denis;Omujal, Francis;Kyeyune, Grace Nambatya;Matsabisa, Motlalepula Gilbert;Kang, Youngmin
    • Journal of Plant Biotechnology
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    • 제49권2호
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    • pp.139-144
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    • 2022
  • The genus Prunus (family: Rosaceae) consists of over 400 plant species and exhibits vast biodiversity worldwide. Given the wide distribution of this genus, its taxonomic classification is important. Anatomical characteristics are conserved and stable and can therefore be used as an important tool for the taxonomic characterization of plants. Therefore, this study aimed to assess and document the anatomical characteristics of the leaf, stem, and seed of P. africana using micrographs and photographs for possible use in the identification, quality control, and phylogenetic analysis of the species. The anatomical sections of a young stem revealed a cortex consisting of isodiametric parenchyma cells, druse crystals, primary vascular bundles, and pith. The mature stem bark majorly consisted of the rhytidome, with the periderm densely arranged in multiple layers; a cluster of stone cells; and sclerenchyma. The leaf sections were hypostomatic, with stomata sizes ranging from 18.90-(22.34)-26.90 × 15.41-(18.40)-21.22 ㎛. The leaf sections showed the presence of characteristic druse crystals, vascular bundles, and mesophyll layers. The pericarp contained the epicarp, mesocarp, and endocarp, with their thickness being approximately 350-400, 300-350, and 30-50 ㎛, respectively. In addition, it contained a seed testa with a thickness of approximately 50-60 ㎛. The morphological and anatomical characteristics observed in P. africana leaves, stems, and seeds in this study could serve as useful data for the taxonomic identification of this species.

한국산 녹나무과 식물의 분류학적 연구 - 화서의 형태 - (Taxonomic Studies of the Lauraceae in Korea - Morphology of Inflorescence -)

  • 선병윤
    • Journal of Plant Biology
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    • 제29권4호
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    • pp.329-340
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    • 1986
  • This study is attemped to discuss the taxonomic significances as have been raised up to now in the study of Lauraceous inflorescence. Lauraceous inflorescences are recognized as panicle, umbel, dichasium, or fascicle by various investigators. Reconsidering the inflorescence or existence of involucre, which has been accepted as important character in the present classification system, it is assumed that the study about them has not so far carried out a deliberate analysis over inflorescent morphology, nor has recognized the existence of involucre. Furthermore, all the materials examined in this study have fugacious or persistent involucres which are leafy, scaly or membraneous. Therefore, it was found that the kinds, fugacity or persistency of involucres are significant in the problems of classification of Laureaceae. Korean Lauraceous inflorescence has evolved into the direction of continuous reduction in its peduncle: from compound dichasium to capitate umbel.

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한국산 제비꽃속의 종분화론적 연구 - 태백제비꽃군의 비교형태학적 연구 - (Biosystematic Study on the Genus Viola in Korea - Comparative Morphology of the Viola albida Complex -)

  • 김경식
    • Journal of Plant Biology
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    • 제34권3호
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    • pp.229-238
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    • 1991
  • The main objective of this study was to clarify the taxonomic status and the speciation mechanism of Viola albida complex in Korea. The complex includes three very closely related taxa, taxonomic ranks of which are much in dispute. These taxa in the complex are sympatric in distribution, and they are very similar in most morphological characteristics. Comparative morphological analyses revealed that they can be distinguished on the basis of leaf shape, although they show complicated patterns of variation in leaf shape. The variation of leaf shape in each taxon appeared to be maintained through developmental process, and extensive cleistogamy and vegetative reproduction in these taxa are suggested as the mechanisms maintaining the variation. Genetic variation in this complex was monomorphic in all 14 loci examined. Palynological study also showed that these taxa have the same pollen morphology. These results strongly suggested that they should be recognized as varieties of V. albida; var, albida, var. chaerophylloides, and var. takahashii. The results also suggested that some individuals that can be assigned to var. takahashii might be hybrids between var. albida and var. chaerophylloides.

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Taxonomic Study on the Lichen Genus Xanthoparmelia (Ascomycotina, Parmeliaceae) in Korea

  • Wang, Xin Yu;Koh, Young-Jin;Hur, Jae-Seoun
    • Mycobiology
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    • 제36권4호
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    • pp.203-210
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    • 2008
  • In previous studies investigating the genus Xanthoparmelia, thirteen different species have been reported from South Korea alone. However, there currently has been no revisional study performed until now. To explore the genus Xanthoparmelia, a phenotypic analysis was performed based on morphological, anatomical and chemical characters, while an investigation of Xanthoparmelia phylogeny was based on nuclear ribosomal (nr) DNA ITS sequences. A thorough examination of the specimens deposited in the Korean Lichen Research Institute (KoLRI) confirmed that eight species of Xanthoparmelia occur inside South Korea. Our analysis further confirmed the colors of the lower surface and medullar chemistry are important taxonomic characters in Xanthoparmelia. This study also presents a detailed description of each species and a key to the genus.