• Animal Systematics, Evolution and Diversity
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• 제19권1호
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• pp.139-147
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• 2003

Burckhardt (1987)는 분명하게 과들을 구분해 주는 형질이 없기 때문에 날개 및 생식기의 기본구조가 비슷한 Aphalaridae (모나무이과)와 Spondyliaspididae (호주나무이과)를 Psyllidae (나무이과)의 아과로 통합하였다. 나무이를 대상으로 형질의 기울기와 진화패턴을 분석하기 위해, 호주를 포함한 전세계의 나무이를 분석하면서 호주나무이아과에 관하여 Burckhardt와는 다른 결과를 얻었다. 특히 호주나무이아과에 대한 분류학적 위치를 검토하기 위해 호주전역에서 채집된 28속 52종의 호주산나무이의 정소를 해부한 결과, 나무이의 정소 소포수 형질은 변이가 1-4개까지 나타났으며, 특히 Spondyliaspidinae (호주나무이아과)는 정소소포수가 공통적으로 1개였다. 호주나무이아과가 최근 신생대에 진화한 Eucalyptus 식물군을 기주로 하고, Gondwana 지역에서 폭발적인 종분화를 하였으며, 이 아과의 속 과 종 모두가 1개의 정소 소포수를 공통적으로 가진다는 점에서 호주나무이아과는 나무이과에서 독립된 호주나무이과로 분리하여야 한다.

• 미생물학회지
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• 제36권3호
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• pp.167-172
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• 2000

제주도의 여섯 지역과 인천 작약도 근해의 해수로부터 내염성 그람양성 세균 25균주를 분리하여 화학 분류학적 특징과 표현형적 특성을 조사하였다. 분리된 균주들은 화학 분류학적 특징에 의거하여 4개의 group으로 구분되었다. Group1은 40.~49.9 mol% 의 G+C함량과 MK-7의 menaquinome type, peptidoglycan의 주요 아미노산으로 meso-A2pm을 함유하며 Bacillus pumilus와 Bacillus licheniformis, Bacillu megaterium, bacillus subtilis로 동정되었으며, Group 2는 63.9~66.4 mol% 의 G+C 함량과 MK-8을 함유하는 Arthrobacter속 세균이었으며 , Group 3은 31.0~37.6 mol%의 G+G 함량과 MK-7을 함유하며 Staphylococcus haemolyticus, Staphylococcus saprophyticyus, Staphylococcus intermedius 로 , Group 4는 72.0 mol% 의 G+C 함량과 MK-8을 주요 quinone으로 함유하는 Micrococcus luteus로 동정되었다. 분리된 세균들 중 Bacillus 속 세균들은 68%로 우세한 분포를 나타내었다.

• Mycobiology
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• 제36권3호
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• pp.143-147
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• 2008

We have detected the slime mold, Diachea leucopodia (GNU06-10) in a strawberry greenhouse located in Sancheong-gun, Gyeongnam. Typical fruiting bodies had developed gregariously on the strawberry leaves, petioles, and plant debris on ground soil habitat, and also surprisingly on plastic pipes and a vinyl covering. Field samples were examined via stereomicroscopy, light microscopy, and SEM for the determination of morphological characteristics. Dark-brown to black spores formed gregariously within the stipitate cylindrical sporangium, and were covered by an iridescent peridium, which may be intact at maturity, or may have disintegrated. The upper portion of the peridium generally breaks up to expose the spores, whereas the lower portion was usually persistent. The results of energy dispersive X-ray spectrometer (EDS) analysis showed that lime was present in the stalk and columella but absent from the spores, capillitium, and peridium. The above characteristics confirm its taxonomic position in the genus Diachea. However, this genus is intermediate in character between the Physarales and Stemonitales of the Myxogastromycetidae. Hence, this genus had been classified as a member of the Stemonitales until the mid-1970's, on the basis of its iridescent peridium and noncalcareous capillitial system, similar to Comatricha of the Stemonitaceae. By way of contrast, emphasis on morphological characteristics, most notably the calcareous stalk and typical columella, places Diachea within the order Physarales. The presence of a phaneroplasmodium during the trophic stage and lime deposition in its sporophores, as was confirmed in this work, supported the inclusion of Diachea in the Physarales, and the noncalcareous capillitial system verified its identification as a member of the Didymiaceae. Further characteristics of the species D. leucopodia include the following: phaneroplasmodium, spore globose 7.5 ${\mu}m$ in diameter, very minutely roughened; sporangia $500{\mu}m\times1mm$, more or less cylindrical, gregarious, stalked 1.2mm; stalk and columella white.

• 한국임상수의학회지
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• 제38권2호
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• pp.75-81
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• 2021

An aquariid nematode, Desportesius invaginatus, was found in the proventriculus of an Egretta garzetta and a Bubulcus ibis from Chuncheon in the Republic of Korea. The worms were identified by light and scanning electron microscopy based on important taxonomic characteristics (body length, esophagus length, cordons, spicules, caudal alae of males, position of the vulva) and then phylogenetically analyzed using the 18S rRNA encoding gene. The nematodes were characterized by a body length of 7.0-8.0 mm in males and 10.2-13.1 mm in females, and two pairs of cordons recurrent in the anterior direction, and cordons were anastomosed by a longitudinal cuticular ridge that externally delimits a longitudinal canal. The widest cuticular plates of cordons bears over 20 posterior spines. The length of the spicules in males was also significantly different. The right spicule measured 742-821 (794) ㎛ in length and 40-45 (42) ㎛ in width, and the left spicule measured 493-556 (541) ㎛ in length and 11-13 (12) ㎛ in width. The caudal alae of males are inflated and vesicular in appearance. The vulva was situated at 56-71 (58.3) ㎛ from the posterior extremity. Although the 18S rRNA sequences of worms were similar to the Synhimantus species, some genetic divergences were observed in comparison. In this study, the worms were recognized with genus Desportesius because genus Desportesius was considered a subgenus of Synhimantus. This is the first record of D. invaginatus in the Republic of Korea.

• Mycobiology
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• 제50권1호
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• pp.46-54
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• 2022

Although Apiospora Sacc. has previously been considered a sexual morph of Arthrinium species on the basis of phylogenetic, morphological, and ecological diagnoses, a recent study delimited these as different species. Recently, 14 species, including eight new species, of marine Arthrinium have been reported from Korea. Six known species have previously been renamed as species in the genus Apiospora (A. arundinis, A. marii, A. piptatheri, A. rasikravindrae, A. sacchari, and A. saccharicola). However, the eight new species of marine Arthrinium (Ar. agari, Ar. arctoscopi, Ar. fermenti, Ar. koreanum, Ar. marinum, Ar. pusillispermum, Ar. sargassi, and Ar. taeanense) are yet to be studied, and thus the taxonomic status of these species remains to be clarified. In this study, we conducted phylogenetic analyses using the internal transcribed spacer, 28S large subunit ribosomal RNA gene, translation elongation factor 1-alpha, and beta-tubulin regions to confirm the phylogenetic position of these eight species. Based on these analyses, we re-identified the eight Arthrinium species as new combinations in Apiospora. Additionally, among the six known Apiospora species, two (A. piptatheri and A. rasikravindrae) have not previously been recorded in Korea. On the basis of morphological and molecular analyses, we report these as new species in Korea. Herein, we present scanning electron micrographs detailing the morphologies of these species, along with phylogenetic trees and detailed descriptions.

• Journal of Microbiology and Biotechnology
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• 제32권12호
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• pp.1553-1560
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• 2022

A Gram-stain-negative, rod-shaped bacterial strain, JC4^T, was isolated from a freshwater sample and determined the taxonomic position. Initial identification based on 16S rRNA gene sequences revealed that strain JC4^T is affiliated to the genus Mucilaginibacter with a sequence similarity of 97.97% to Mucilaginibacter rigui WPCB133^T. The average nucleotide identity and digital DNA-DNA hybridization values between strain JC4^T and Mucilaginibacter species were estimated below 80.92% and 23.9%, respectively. Strain JC4^T contained summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c) and iso-C_15:0 as predominant cellular fatty acids. The dominant polar lipids were identified as phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, and two unidentified lipids. The respiratory quinone was MK-7. The genomic DNA G+C content of strain JC4^T was determined to be 42.44%. The above polyphasic evidences support that strain JC4^T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter aquariorum sp. nov. is proposed. The type strain is JC4^T (= KCTC 92230^T = LMG 32715^T).

• Journal of Species Research
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• 제12권2호
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• pp.165-173
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• 2023

Bacterial communities inhabiting islands play a vital role in the functioning and formation of a unique, isolated ecosystem. Nevertheless, there has been a lack of systematic research on the indigenous microbiological resources of the islands in Korea. To excavate microbial resources for further studies on the metabolism and biotechnological potential, a standard dilution plating was applied to coastal seawater samples collected from islands along the west coast of the Korean Peninsula, including Deokjeokdo, Baengnyeongdo, and Daebudo in 2022. A total of 2,007 bacterial strains were isolated from the samples as single colonies and identified using 16S rRNA gene sequence analyses. A total of 20 strains, with ≥98.7% 16S rRNA gene sequence similarity to bacterial species having validly published names but not reported in Korea, were designated as unrecorded bacterial species in Korea. The unrecorded bacterial strains were phylogenetically diverse and belonged to four phyla, five classes, 12 orders, 17 families, and 18 genera. The unreported species were assigned to Algimonas, Amylibacter, Notoacmeibacter, Roseibium, and Terasakiella of the class Alphaproteobacteria; Alteromonas, Congregibacter, Marinagarivorans, Marinicella, Oceanospirillum, Psychromonas, Thalassotalea, Umboniibacter, and Vibrio of the class Gammaproteobacteria; Lutibacter and Owenweeksia of the class Flavobacteriia; Paenibacillus of the class Bacilli; and Pelagicoccus of the class Opitutae. The taxonomic characteristics of the unreported species, including morphology, biochemistry, and phylogenetic position are provided in detail.

• Journal of Species Research
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• 제13권3호
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• pp.318-325
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• 2024

Bacterial communities residing on islands have a significant impact on the functioning and establishment of a unique isolated ecosystem. Notwithstanding, systematic research on the indigenous microbial resources of domestic islands has been lacking. In order to understand the biodiversity and potential bioresources, we conducted sampling in 2023 from coastal waters from various islands off the west coast of the Korean Peninsula, including Baengnyeongdo, Daebudo, Deokjeokdo, Jangbongdo, Yeonpyeongdo Islands, along with Somaemuldo Island along the south coast. The coastal seawater samples were used to unearth microbial resources through the standard dilution plating. In total, approximately 1,600 bacterial strains were isolated from the samples as single colonies and identified using 16S rRNA gene sequence analyses. Eighteen strains, exhibiting ≥98.7% 16S rRNA gene sequence similarity to bacterial species with validly published names but not previously reported in Korea, were categorized as unrecorded bacterial species in Korea. These unrecorded bacterial strains displayed phylogenetic diversity, representing three phyla, four classes, 9 orders, 13 families, and 18 genera. The unrecorded species were assigned to the classes Alphaproteobacteria (Aliiroseovarius, Kiloniella, Maritalea, Palleronia, and Roseobacter), Gammaproteobacteria (Aliamphritea, Aliivibrio, Enterovibrio, Francisella, Leucothrix, Pseudoalteromonas, Psychrobium, Shewanella, and Vibrio), Flavobacteriia (Aquimarina, Pseudofulvibacter, and Tenacibaculum), and Verrucomicrobiae (Roseibacillus). This study presents comprehensive descriptions of the taxonomic attributes of these unrecorded species, covering morphology, biochemistry, and phylogenetic position.

• 식물분류학회지
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• 제45권4호
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• pp.306-317
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• 2015

본 연구에서는 간혹 흰바위취(Micranthes manchuriensis)와 동일종으로 취급되기도 하는 한국 고유종 구실바위취(Saxifraga octopetala)의 분류학적 지위를 알아보고자 수행되었다. 구실바위취의 계통학적 위치와 종의 경계를 확인하기 위해 두 종의 기준표본에 대한 형태적 검토와 핵 리보솜 DNA의 ITS 지역 염기서열에 대한 집중적인 계통분석을 시행하였다. 총 65개 구실바위취 개체는 ITS 계통수에서 하나의 무리를 이루면서 Micranthes 분기군(clade)에 포함되었고, 톱바위취 및 흰바위취와 가까운 계통 유연관계를 나타내었다. 중국과 러시아에서 채집된 다수의 흰바위취 개체 역시 독자적인 분기군을 형성하면서 M. nelsoniana var. pacifica 및 M. fusca와 자매군을 이루었다. 구실바위취의 실체가 모호했던 것은 흰바위취 개체와 톱바위취의 화서를 함께 포함하고 있는 복합표본인 Wilford의 채집품(흰바위취의 기준표본)을 Nakai가 잘못 관찰하였기 때문으로 생각되었다. 구실바위취와 흰바위취의 높은 형태적 유사성에도 불구하고 이들은 지하 포복경의 특징에 있어서 차이를 보였다. ITS 계통수에서 구실바위취가 Micranthes 내에 자리하는 것으로 나타나 구실바위취는 Micranthes 내에서 종의 지위를 유지하여야 할 것으로 판단되었고, 이에 따라 신조합명(Micranthes octopetala)을 발표하였다.

• 식물분류학회지
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• 제38권2호
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• pp.79-91
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• 2008

일본, 중국의 남서지방과 히말라야에 분포하는 Asplenium yoshinagae Makino (가거꼬리고사리: 신칭)가 전남 신안군 흑산면 가거도에서 발견되었다. 이 분류군은 무성아를 가지며 우편에 귀가 발달하는 점에서 꼬리고사리속 내에서 차꼬리고사리, 개차꼬리고사리, 깃고사리, 반들깃고사리, 개차고사리와 유사하나, 잎자루와 우편자루가 뚜렷이 발달하는 점, 우편의 끝이 뾰족한 점, 포막의 길이와 포자낭군의 모양이 다른 점들로 상기 종들과 구별된다. 가거꼬리고사리의 분류학적 위치를 파악하기 위하여 꼬리고사리속 22분류군과 군외군으로 버들참빗속 2분류군을 포함하여 24분류군을 대상으로 20개의 형태형질에 의한 maximum parsimony tree와 neighbor-joining tree를 작성하였다. 분석결과 꼬리고사리속은 군외군과 강하게 분리되었으며, 4개의 군으로 나누어 졌다. 가거꼬리고사리는 3번째군에 속하였으며, 2번째 군에 속하는 A. hondoense N. Murtakami & S. I. Hatanaka는 Hymenasplenium으로 다룬 적이 있으나 본 형태 분석결과 Asplenium속에 속하였고, Asplenium ruprechtii 도 Comptosorus속으로 다루기 보다는 Asplenium속에 속하였다. 아울러 가거꼬리고사리의 형태적 특징을 기재하였고, 도해와 생태 사진을 첨부하였다.