• Biomedical Science Letters
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• v.17 no.4
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• pp.355-361
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• 2011

We evaluated the performance of the NOW $Streptococcus$ $pneumoniae$ urinary antigen test, standard culture and polymerase chain reaction for detecting $S.$ $pneumoniae$. The urinary antigen test of pneumonia patients represented sensitivity at 72% and specificity at 79%. The results of PCR were targeting for autolysin ($lyt$A), pneumolysin ($ply$), and spn9828. The $lyt$A sensitivity and specificity stood at 56% and 87% respectively while $ply$ sensitivity reported 83% and specificity was 47%, sensitivity and specificity of spn9828 stood at 83% and 73% respectively. The results of urinary antigen test and three genes were all statistically meaningful within $P$ <0.05. When the urinary antigen test of $S.$ $pneumoniae$ was positive, the three kinds of genes were also likely to be positive. According to the result of urinary antigen test, the results of PCR presented a meaningful difference ($P$ <0.05). Especially, the urinary antigen test of $S.$ $pneumoniae$ was likely to be positive ($P$ <0.05) when more than two genes were positive in PCR results.

• Journal of the Korea Society for Simulation
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• v.28 no.2
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• pp.81-88
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• 2019

Since the AESA radar scans and tracks a distant targets or ground, it requires a test field which meets far-field condition before flight test. In order to test beam foaming, targeting, and availability from cluttering and jamming, it is general to build a outdoor roof-lab test site at tens of meters high. However, the site is affected by surrounding terrain, weather, and noise wave and is also requires time, space, and a lot of costs. In order to solve this problem, theoretical near-field beam foaming method has proposed. However, it requires modification of associated hardware in order to construct near-field test configuration. In this paper, we propose near-field beam foaming method which use single LUT in order to calibrate the variation of TRM(transmit-receive module) which consists AESA radar without modification of associated hardware and software. It requires less costs than far-field test and multiple LUT based near-field test, nevertheless it can derives similar experimental results.

• Journal of Korean Society of Forest Science
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• v.108 no.1
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• pp.109-115
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• 2019

The objectives of this study was to analyze the importance and satisfaction (ISA) of forest healing instructor training program, targeting on the students educated in 2018. As a result, it was found that students older than forties are the majority as 81 %, and they preferred forest healing instructors as their second future job. When comparing the importance and satisfaction, the students evaluated satisfaction with low grade compared to importance with high grade in all questions. In ISA analysis, it was needed to improve the updated textbook information, to list the example problems by subject, to expose the test subjects in the textbooks, to disclose the test questions and answers, to control the test difficulty, to adjust the test pass rate, to expand the number of test and test sites, to increase the effort for making students understand, to prepare lectures appropriate to practice for forest healing instructor training program by priority.

• Journal of Technologic Dentistry
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• v.45 no.4
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• pp.118-123
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• 2023

Purpose: This study proposes measures needed to implement a job-based national practical exam for dental technicians. Methods: For this study, a survey was conducted targeting 244 currently employed individuals. The current national practical test for dental technicians was divided into directions for the job-based practical test, subjects that need improvement among the current practical test subjects, items that need to be reflected in the practical test evaluation for each subject, and subjects that need to be added to the practical test. It comprised ten questions (Cronbach's α=0.801). Results: The following results were obtained. Satisfaction with the current practical test was lower than average. Future improvement should focus on job-based evaluation. The dental ceramic lab technology subject needs to be reorganized urgently, and the tasks that require evaluation for each subject are different. Moreover, if evaluation subjects are added in the future, the introduction of assessment for dental CAD/CAM (computer-aided design/computer-aided manufacturing) subjects was found to be the most urgent. Conclusion: In this study, the most necessary tasks of the current national practical examination for dental technicians were evaluated and the content needed for future reorganization was investigated. Future tests should be expanded to evaluate critical job areas. Furthermore, it is necessary to open new courses in fields such as CAD/CAM, as they are in high demand in the workplace.

• Journal of Korean Biological Nursing Science
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• v.15 no.3
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• pp.147-153
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• 2013

Purpose: This study was conducted in order to closely examine the effects on bone mineral density and health promotion behaviors following the intervention of a 20-week Thera band exercise program targeting elderly women. Methods: This study is a pseudo-experimental research with a pre-and-post design in a single group that examined the effects after the intervention of Thera Band Exercise for 20 weeks targeting 31 elderly women over 60 years of age who visit a Senior Welfare Center located in K city. It analyzed subjects' general characteristics using error and percentage, health promotion behavior, and a bone density aspect using the mean and standard deviation, as well as a change in the health promotion behavior and bone density, before and after exercise with Paired t-test by using the SPSS 18.0 program. Results: The effect of the Thera band exercise program on bone mineral density was reduced (t=8.140, p<.001) gradually after 20 weeks of exercise compared to the pre-exercise period. The health promotion behavior before and after exercise showed the significantly increasing outcomes (t=3.26, p=.003). Conclusion: There is a limitation to a direct rise in bone mineral density with the use of Thera band exercise intervention in the elderly. A change in health promotion behavior before and after exercise increased significantly. Thus, it was confirmed that taking exercise leads to a gradual rise in health promotion behavior. Hence, it understood that continuous exercise is useful and brings about a positive change in promoting health maintenance in elderly women.

• Journal of Microbiology
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• v.40 no.4
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• pp.274-281
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• 2002

The competitive quantitative PCR method targeting pcbC gene was developed for monitoring 4-chlorobiphenyl(4CB)-degrading bacteria, Pseudomonas sp. strain DJ-12, in soil microcosms. The method involves extraction of DNA from soil contaminated with 4CB, PCR amplification of a pcbC gene fragment from the introduced strain with a set of strain-specific primers, and quantification of the elec-trophoresed PCR product by densitometry. To test the adequacy of the method, Pseudomonas sp. strain DJ-12 was introduced into both contaminated and non-contaminated soil microcosms amended with 4CB. Pseudomonas sp. strain DJ-12 was monitored and quantified by a competitive quantitative PCR in comparison with 4CB degradation and the result was compared to those obtained by using the conventional cultivation method. We successfully detected and monitored 4CB-degrading bacteria in each microcosm and found a significant linear relationship between the number of 4CB-degrading bacteria and the capacity for 4CB biodegradation. The results of DNA spiking and cell-spreading experiments suggest that this competitive quantitative PCR method targeting the pcbC gene for monitoring 4CB- degrading bacteria appears to be rapid, sensitive and more suitable than the microbiological approach in estimating the capacity of 4CB biodegradation in environmental samples.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.3
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• pp.270-275
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• 2016

The characteristics of the fuel quantity, injection rate and macro spray development was investigated under a range of diesel fuel temperatures. The actual injection quantity decreased despite the same signal of the injection start and injection duration as the fuel temperature decreased. The injection rate measurements confirmed that the actual injection commencement was delayed and the actual injection duration was shortened under lower fuel temperature conditions, which explains why the injection quantity decreased. Spray tip penetration with a lower fuel temperature was longer than that with a higher fuel temperature due to the deteriorated atomization. As a pre-test for the combustion experiment under low temperature conditions, piston targeting with pilot injection was accomplished, which showed that the fuel droplet from pilot injection was introduced into the crevice area. This suggests that the pilot injection quantity and timing should be chosen with careful consideration for actual applications.

• Molecules and Cells
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• v.40 no.11
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• pp.823-827
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• 2017

Genome editing using programmable nucleases such as CRISPR/Cas9 or Cpf1 has emerged as powerful tools for gene knock-out or knock-in in various organisms. While most genetic diseases are caused by point mutations, these genome-editing approaches are inefficient in inducing single-nucleotide substitutions. Recently, Cas9-linked cytidine deaminases, named base editors (BEs), have been shown to convert cytidine to uridine efficiently, leading to targeted single-base pair substitutions in human cells and organisms. Here, we first report on the generation of Xenopus laevis mutants with targeted single-base pair substitutions using this RNA-guided programmable deaminase. Injection of base editor 3 (BE3) ribonucleoprotein targeting the tyrosinase (tyr) gene in early embryos can induce site-specific base conversions with the rates of up to 20.5%, resulting in oculocutaneous albinism phenotypes without off-target mutations. We further test this base-editing system by targeting the tp53 gene with the result that the expected single-base pair substitutions are observed at the target site. Collectively, these data establish that the programmable deaminases are efficient tools for creating targeted point mutations for human disease modeling in Xenopus.

• Polymer(Korea)
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• v.31 no.5
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• pp.454-459
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• 2007

Gene therapy using low molecular weight water soluble chitosan (LMWSC) as polycationic polymer shows good biocompatibility, but low transfection efficiency. The mechanism of folic acid (FA) uptake in the cells to promote targeting and internalization could improve transfection rates. The objective of this study was to synthesize and characterize the WSCFA-DNA complex and evaluate their cytotoxicity, in vitro. In $^1H-NMR$ spectra, specific peaks appeared both of FA and LMWSC in $D_2O$. WSCFA nanoparticles have spherical shapes with particle size show below 110 nm. In the cell cytotoxicity test, the WSCFA-DNA complex showed high cell viability, in vitro. Gel electrophoresis showed condensed DNA within the carriers. hi vitro transfection efficiency was assayed by fluorescence spectroscopy WSCFA nanoparticles have less cytotoxicity, good DNA condensation and particle size around 110 nm, which makes them a promising candidate as a non-viral gene vector.

• Bulletin of the Korean Chemical Society
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• v.31 no.11
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• pp.3333-3340
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• 2010

A three dimensional pharmacophore model was generated for the molecules which are responsible for anti-inflammatory activities targeting Interleukin-2 inducible tyrosine kinase (Itk). 16 structurally diverse molecules were selected as training set to generate the hypotheses using Discovery Studio v2.1. The best hypothesis, Hypo1, comprises two hydrogen bond acceptor (HBA), one hydrophobic aromatic (HA), one ring aromatic (RA) and shows high cost difference (63.71), high correlation coefficient (0.97) as well as low RMS deviation (0.81). Hypo1 has been further validated toward a test set, decoy set and Fischer's randomization method. Furthermore, Hypo1 was used to screen NCI and Maybridge databases. Finally, 2 hit molecules were identified as potential leads against Itk, which may be useful for future drug development.