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Characterization and Preparation of Low Molecular Weight Water Soluble Chitosan Nanoparticle Modified with Cell Targeting Ligand for Efficient Gene Delivery  

Heo, Sun-Heang (Department of Nano Polymer Science and Engineering, Sunchon National University)
Jang, Min-Ja (Department of Nano Polymer Science and Engineering, Sunchon National University)
Kim, Dong-Gon (Department of Nano Polymer Science and Engineering, Sunchon National University)
Jeong, Young-Il (Department of Nano Polymer Science and Engineering, Sunchon National University)
Jang, Mi-Kyeong (Department of Nano Polymer Science and Engineering, Sunchon National University)
Nah, Jae-Woon (Department of Nano Polymer Science and Engineering, Sunchon National University)
Publication Information
Polymer(Korea) / v.31, no.5, 2007 , pp. 454-459 More about this Journal
Abstract
Gene therapy using low molecular weight water soluble chitosan (LMWSC) as polycationic polymer shows good biocompatibility, but low transfection efficiency. The mechanism of folic acid (FA) uptake in the cells to promote targeting and internalization could improve transfection rates. The objective of this study was to synthesize and characterize the WSCFA-DNA complex and evaluate their cytotoxicity, in vitro. In $^1H-NMR$ spectra, specific peaks appeared both of FA and LMWSC in $D_2O$. WSCFA nanoparticles have spherical shapes with particle size show below 110 nm. In the cell cytotoxicity test, the WSCFA-DNA complex showed high cell viability, in vitro. Gel electrophoresis showed condensed DNA within the carriers. hi vitro transfection efficiency was assayed by fluorescence spectroscopy WSCFA nanoparticles have less cytotoxicity, good DNA condensation and particle size around 110 nm, which makes them a promising candidate as a non-viral gene vector.
Keywords
LMWSC; folic acid; nanoparticle; gene therapy;
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