• Journal of the Korea Society of Computer and Information
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• v.19 no.4
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• pp.149-158
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• 2014

The rapidly changing 21st-century knowledge and information society is emphasizing converged education that crosses various academic fields. In particular, the society expected the cultivation of the talent who balance scientific creativity and artistic sensitivity by adding arts to the existing converged education revolving around science and technology. However, at present, most STEAM education has been actively conducted with a focus on science and technology, whereas the subject of arts has been regarded or utilized as a supplementary means. Its problem is that the educational characteristics and values of art education have not been effectively utilized in educational terms and this could lead to superficial integrated education. In this respect, this study had the knowledge of various fields, such as science, technology, and mathematics, utilized usefully during the process of experiencing and creating arts. Accordingly, this study designed an education programs as with the case of Nam-Jun Baek who expanded the dominion of arts by creatively utilizing his own time's scientific technologies. In this educational process, the target program was developed in a manner that enables EPL to be utilized essentially as the study's knowledge-based tool and medium. The results of applying this educational program in 5th-grade elementary school students showed that the program has positive effects on the creative attributes of the students.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.2
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• pp.102-102
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• 2014

The NISS (Near-infrared Imaging Spectrometer for Star formation history) onboard NEXTSat-1 is the near-infrared instrument onboard NEXTSat-1 which is being developed by KASI. The main scientific targets are nearby galaxies, galaxy clusters, star-forming regions and low background regions in order to study the cosmic star formation history in local and distant universe. After the Preliminary Design Review, we have fixed major specifications of the NISS. The off-axis optical design with 15cm apertureis optimized to obtain a wide field of view ($2deg.{\times}2deg.$), while minimizing the sensitivity loss. The opto-mechanical structure of the NISS was designed to be safe enough to endure in the launching condition as well as the space environment. The tolerance analysis was performed to cover the wide wavelength range from 0.95 to $3.8{\mu}m$ and to reduce the degradation of optical performance due to thermal variation at the target temperature, 200K. The $1k{\times}1k$ infrared sensor is operated in the dewar at 80K stage. We confirmed that the NISS can be cooled down to below 200K in the nominal orbit through a radiative cooling. Here, we report the preliminary design of the NISS.

• Research in Plant Disease
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• v.17 no.2
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• pp.184-190
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• 2011

Ralstonia solanacearum, a causal agent of bacterium wilt is very difficult to control once the disease becomes endemic. Thus, Ralstonia solanacearum is a plant quarantine bacterium in many countries including Korea. In this study, we developed PCR assays, which can detect Ralstonia solanacearum from the Solanaceae seeds. Primers RS-JH-F and RS-JH-R amplified specifically a 401 bp fragment only from Ralstonia solanacearum race 1 and race 3. The nested PCR primers, RS-JH-F-ne and RS-JH-R-ne that were designed inside of 1st PCR amplicon amplified specifically a 131 bp fragment only from Ralstonia solanacearum race 1 and race 3. The primers did not amplify any non-specific DNA from the seed extracts of the Solanaceae including tomato and pepper. When detection sensitivity were compared using the Solanaceae seeds inoculated with target bacteria artificially, the nested PCR method developed in this study 100 times more sensitive than ELISA and selective medium. Therefore, we believe that the PCR assays developed in this work is very useful to detect Ralstonia solanacearum in the Solanaceae seeds.

• Journal of the Korean Society for Nondestructive Testing
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• v.27 no.1
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• pp.31-37
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• 2007

A $^{192}Ir$ small-focal source has been developed by using the HANARO reactor and the radioisotope production facility at the Korea Atomic Energy Research Institute (KAERI). The small-focal source with the dimension of 0.5 mm in diameter and 0.5 mm in length was fabricated as an aluminum-encapsulated form by a specially designed pressing equipment. For the estimation of the radioactivity, neutron self-shielding and ${\gamma}-ray$ self-absorption effects on the measured activity was considered. From this estimation, it is realized that $^{192}Ir$ small-focal sources over 3 Ci activities can be produced from the HANARO. Field performance tests were performed by using a conventional source and the developed source to take images of a computer CPU and a piece of a carbon steel. The small-focal source showed better penetration sensitivity and geometrical sharpness than the conventional source does. It is concluded from the tests that the focal dimension of this source is small enough to maximize geometrical sharpness in the image taking for the close proximity shots, pipeline crawler applications and contact radiography.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.11
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• pp.6605-6611
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• 2013

Background: WEE1 is a G2/M checkpoint regulator protein. Various studies have indicated that WEE1 could be a good target for cancer therapy. The main aim of this study was to asssess the tumor suppressive potential of WEE1 silencing in two different breast cancer cell lines, MCF7 which carries the wild-type p53 and MDA-MB468 which contains a mutant type. Materials and Methods: After WEE1 knockdown with specific shRNAs downstream effects on cell viability and cell cycle progression were determined using MTT and flow cytometry analyses, respectively. Real-time PCR and Western blotting were conducted to assess the effect of WEE1 inhibition on the expression of apoptotic (p53) and anti-apoptotic (Bcl2) factors and also a growth marker (VEGF). Results: The results showed that WEE1 inhibition could cause a significant decrease in the viability of both MCF7 and MDA-MB-468 breast cancer cell lines by more than 50%. Interestingly, DNA content assays showed a significant increase in apoptotic cells following WEE1 silencing. WEE1 inhibition also induced upregulation of the apoptotic marker, p53, in breast cancer cells. A significant decrease in the expression of VEGF and Bcl-2 was observed following WEE1 inhibition in both cell lines. Conclusions: In concordance with previous studies, our data showed that WEE1 inhibition could induce G2 arrest abrogation and consequent cell death in breast cancer cells. Moreover, in this study, the observed interactions between the pro- and anti-apoptotic proteins and decrease in the angiogenesis marker expression confirm the susceptibility to apoptosis and validate the tumor suppressive effect of WEE1 inhibition in breast cancer cells. Interestingly, the levels of the sensitivity to WEE1 silencing in breast cancer cells, MCF7 and MDA-MB468, seem to be in concordance with the level of p53 expression.

• Journal of Convergence for Information Technology
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• v.10 no.6
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• pp.156-163
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• 2020

This study made a final analysis of 184 people in their 40s and 50s living in South Gyeongsang Province to identify their relevance to oral health education experiences, oral health beliefs, oral efficacy, oral hygiene product use practices, and quality of life. When there was educational experience in oral efficacy and practice of oral hygiene product use according to oral health education, oral efficacy, oral hygiene product use practice and quality of life was high.In the relationship between oral health belief and oral efficacy, oral hygiene product use practice and quality of life, we could see that oral efficacy and quality of life were low when sensitivity and severity were high among oral health belief, and that oral efficacy and quality of life were high when importance, disability, and benefits were high. Middle age based on oral health care the results over the continuing and developers to develop customized education programs for different target for a longer term recurrent training and future.A Study on the Application through the program will be needed.

• The Journal of Internal Korean Medicine
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• v.19 no.1
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• pp.221-246
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• 1998

The aim of this experimental study was to evaluate the anti-tumor effects of Chungsimbohyeltang through investigation about viability of tumor cell by MTT assay, survival period of mice transplanted with L-1210 cells, growth inhibition on the tumor cell, body weight variation in mice transplanted with sarcoma-180 cells and its immunomodulatory effects through the investigation on delayed type hyper-sensitivity, the hemagglutinin and hemolysin titers for humoral immune response, the appearance of rosette forming cells for cell-mediated immune response, the natural killer cell activity, the transformation of lymphocyte, the productivity of Interleukin-2 and phagocytic index K was performed in immune-depressed ICR mice induced by methotrexate treatments. The results were as follows ; 1. $IC_{50}$ of Chungsimbohyeltang treated group was 5.85mg/ml in SNU-C4 cell, 1.38mg/ml in SNU-396 cell, 0.21mg/ml in SNU-1 cell, so it had low anti-tumor activity. 2. The both groups of Chungsimbohyeltang extract 10mg/kg and Chungsimbohyeltang extract 20mg/kg had no toxicity and the group of Chungsimbohyeltang 20mg/kg which was shown 120% in ILS had the effect of life prolongation in mice transplanted with L-1210 cells. 3. In the growth inhibition on the tumor cells, only the group of Chungsimbohyeltang extract 20mg/kg was noted and in the weight variation in mice transplanted with sarcoma-180 cells, both groups of Chungsimbohyeltang extract had a significant effect. 4. In the delayed type hypersensitivity and appearance of rosette forming cells, both groups of Chungsimbohyeltang extract didn't have any significant effect. 5. The hemagglutinin titers was slightly increased with no significance, and the hemolysin titers was significantly increased in the only group of Chungsimbohyeltang extract 20mg/kg. 6. The natural killer cell activity of the Chungsimbohyeltang extract groups was significantly increased in the ratio of 100:1 of effector and target cells, but it was not significantly increased in the ratio of 50:1, 10:1. 7. The transformation of lymphocyte and the productivity of Interleukin-2 were increased significantly and in dose-dependent manner in both group of Chungsimbohyeltang extract. 8. The phagocytic effect of macropage was significantly increased in both groups of Chungsimbohyeltang extract. Considering the results above, we could conclude that Chungsimbohyeltang have an indirect anti-tumor effect through the modulation of immunme response, although it had not toxicity on the tumor cell it self.

• The Journal of the Korea Contents Association
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• v.16 no.7
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• pp.476-483
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• 2016

Epicardial adipose tissue(EAT) is metabolically active endocrine organ that secretes several hormones in fat thickness is a risk factor for cardiovascular disease and metabolic disorders. This study was to measure and then using ultrasound epicardial adipose tissue thickness, abdominal subcutaneous fat thickness in the target group correlates and general blood properties and characteristics, and presents a local thickness for prediction of metabolic disorders. Results epicardal adipose tissue of the average thickness measured in each of the subjects was 8.890mm, 4.783mm, 4.777, 6.147mm in each section. Showed the epicardial adipose tissue in correlation with the average thickness of the risk factors age, BMI, SBP, LDH, LDL, TC is a positive correlation relationship(p<0.05) in each section. In particular, the thickness of the metabolic disorders epicardial adipose tissue thickness, abdominal subcutaneous compared to subjects that do not have the risk subjects with a risk factor for fat significantly higher(p<0.05). It showed the most reliable that can be cut-off value of 8.950mm obtained with 66.7 % sensitivity and 80 % specificity for predicting the risk of metabolic disorders.

• Journal of Korean Neurosurgical Society
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• v.52 no.6
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• pp.534-540
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• 2012

Objective : Brainstem arteriovenous malformation (AVM) is rare and radiosurgical management is complicated by the sensitivity of the adjacent neurological structures. Complete obliteration of the nidus is not always possible. We describe over 20 years of radiosurgical procedures for brainstem AVMs, focusing on clinical outcomes and radiosurgical techniques. Methods : Between 1992 and 2011, the authors performed gamma knife radiosurgery (GKRS) in 464 cerebral AVMs. Twenty-nine of the 464 patients (6.3%) reviewed had brainstem AVMs. This series included sixteen males and thirteen females with a mean age of 30.7 years (range : 5-71 years). The symptoms that led to diagnoses were as follows : an altered mentality (5 patients, 17.3%), motor weakness (10 patients, 34.5%), cranial nerve symptoms (3 patients, 10.3%), headache (6 patients, 20.7%), dizziness (3 patients, 10.3%), and seizures (2 patients, 6.9%). Two patients had undergone a previous nidus resection, and three patients had undergone a previous embolization. Twenty-four patients underwent only GKRS. With respect to the nidus type and blood flow, the ratio of compact type to diffuse type and high flow to low flow were 17 : 12 and 16 : 13, respectively. In this series, 24 patients (82.8%) had a prior hemorrhage. The mean target volume was 1.7 $cm^3$ (range 0.1-11.3 $cm^3$). The mean maximal and marginal radiation doses were 38.5 Gy (range 28.6-43.6 Gy) and 23.4 Gy (range 18-27 Gy), and the mean isodose profile was 61.3% (range 50-70%). Results : Twenty-four patients had brainstem AVMs and were followed for more than 3 years. Obliteration of the AVMs was eventually documented in 17 patients (70.8%) over a mean follow-up period of 77.5 months (range 36-216 months). With respect to nidus type and blood flow, the obliteration rate of compact types (75%) was higher than that of diffuse types (66.7%), and the obliteration rate of low flow AVMs (76.9%) was higher than that of high flow AVMs (63.6%) (p<0.05). Two patients (6.9%) with three hemorrhagic events suffered a hemorrhage during the follow-up period. The annual bleeding rate of AVM after GKRS was 1.95% per year. No adverse radiation effects or delayed cystic formations were found. Conclusion : GKRS has an important clinical role in treatment of brainstem AVMs, which carry excessive surgical risks. Angiographic features and radiosurgical techniques using a lower maximal dose with higher isodose profiles are important for lesion obliteration and the avoidance of complications.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.12
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• pp.117-124
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• 2019

Vibrio cholerae is a very important pathogenic bacterium that has to be monitored in seafood and ships' ballast water. Various methods have been developed to identify this bacterium, yet these methods are time-consuming and have limitations for their sensitivity to detect contamination. The purpose of the present study was to develop a robust and reliable method for identifying V. cholerae. Peptide nucleic acid (PNA) probes were developed to use for PNA-based asymmetrical real-time PCR techniques. The toxigenic Cholera enterotoxin subunit B (ctxB) gene was selected as a target for detecting V. cholerae and the gene was synthesized as a positive template for conventional and real-time PCR. Real-time PCR primers and PNA probes were designed and standard curves were produced for the quantitative analysis. The selected PNA probes reacted specifically to V. cholerae without any ambiguity, even among closely related species, and the detection limit was 0.1 cfu/100 mL. Taken together, the PNA probes and asymmetrical qPCR methods developed in this present study could contribute to the rapid, accurate monitoring of V. cholerae in marine environments, and as well as in seafood and ships' ballast waters.