• Journal of Institute of Control, Robotics and Systems
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• v.12 no.5
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• pp.431-437
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• 2006

In this paper vector code correlation(VCC) method and an algorithm to promote the image-processing performance in building an effective measurement system using cameras are described far automatically berthing and controlling the ship equipped with side-thrusters. In order to realize automatic ship berthing, it is indispensable that the berthing assistant system on the ship should continuously trace a target in the berth to measure the distance to the target and the ship attitude, such that we can make the ship move to the specified location. The considered system is made up of 4 apparatuses compounded from a CCD camera, a camera direction controller, a popular PC with a built-in image processing board and a signal conversion unit connected to parallel port of the PC. The object of this paper is to reduce the image-processing time so that the berthing system is able to ensure the safety schedule against risks during approaching to the berth. It could be achieved by composing the vector code image to utilize the gradient of an approximated plane found with the brightness of pixels forming a certain region in an image and verifying the effectiveness on a commonly used PC. From experimental results, it is clear that the proposed method can be applied to the measurement system for automatic ship berthing and has the image-processing time of fourfold as compared with the typical template matching method.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.37 no.3
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• pp.267-274
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• 2009

Recently, UAVs (Unmanned Aerial Vehicles) are expected much as the Unmanned Systems for various missions. These missions are often based on the Vision System. Especially, missions such as surveillance and pursuit have a process which is carried on through the transmitted vision data from the UAV. In case of small UAVs, monocular vision is often used to consider weights and expenses. Research of missions performance using the monocular vision is continued but, actually, ground and target model have difference in distance from the UAV. So, 3D distance measurement is still incorrect. In this study, Mean-Shift Algorithm, Optical Flow and Subspace Method are posed to estimate the relative depth. Mean-Shift Algorithm is used for target tracking and determining Region of Interest (ROI). Optical Flow includes image motion information using pixel intensity. After that, Subspace Method computes the translation and rotation of image and estimates the relative depth. Finally, we present the results of this study using images obtained from the UAV experiments.

• Korean Journal of Environmental Agriculture
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• v.40 no.3
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• pp.186-193
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• 2021

BACKGROUND: Before generating transgenic plant using the CRISPR-Cas9 system, the efficiency test of sgRNAs is recommended to reduce the time and effort for plant transformation and regeneration process. The efficiency of the sgRNA can be measured through the transient expression of sgRNA and Cas9 gene in tomato cotyledon; however, we found that the calculated efficiency showed a large variation. It is necessary to increase the precision of the experiment to obtain reliable sgRNA efficiency data from transient expression. METHODS AND RESULTS: The cotyledon of 11^th, 15^th, 19^th, and 23^rd-day-old tomato (Solanum lycopersicum cv. Micro-Tom) were used for expressing CRISPR-Cas9 transiently. The agrobacterium harboring sgRNA for targeting ALS2 gene of tomato was injected through the stomata of leaf adaxial side and the genomic DNA was extracted in 5 days after injection. The target gene edition was identified by amplifying DNA fragment of target region and analyzing with Illumina sequencing method. The target gene editing efficiency was calculated by counting base deletion and insertion events from total target sequence read. CONCLUSION: The CRISPR-Cas9 editing efficiency varied with tomato cotyledon age. The highest efficiency was observed at the 19-day-old cotyledons. Both the median and mean were the highest at this stage and the sample variability was also minimized. We found that the transgene of CRISPR-Cas9 system was strongly correlated with plant leaf development and suggested the optimum cotyledon leaf age for Agrobacterium-mediated transfection in tomato.

• Nuclear Engineering and Technology
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• v.54 no.12
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• pp.4698-4707
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• 2022

As part of the ICRP Task Group 103 project, we developed ten thyroid models for the pediatric mesh-type reference computational phantoms (MRCPs). The thyroid is not only a radiosensitive target organ needed for effective dose calculation but an important source region particularly for radioactive iodines. The thyroid models for the pediatric MRCPs were constructed by converting those of the pediatric voxel-type reference computational phantoms (VRCPs) in ICRP Publication 143 to a high-quality mesh format, faithfully maintaining their original topology. At the same time, we improved several anatomical parameters of the thyroid models for the pediatric MRCPs, including the mass, overlying tissue thickness, location, and isthmus dimensions. Absorbed doses to the thyroid for the pediatric MRCPs for photon external exposures were calculated and compared with those of the pediatric VRCPs, finding that the differences between the MRCPs and VRCPs were not significant except for very low energies (<0.03 MeV). Specific absorbed fractions (target ⟵ thyroid) for photon internal exposures were also compared, where significant differences were frequently observed especially for the target organs/tissues close to the thyroid (e.g., a factor of ~1.2-~327 for the thymus as a target) due mainly to anatomical improvement of the MRCP thyroid models.

• Journal of the Korea institute for structural maintenance and inspection
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• v.20 no.3
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• pp.111-121
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• 2016

In this study, measurements were conducted by image processing to do an in-depth evaluation of strain of rebar in a uniaxial tension test. The distribution of strain and the necking region were evaluated. The image processing is used to analyze the color information of a colored image, so that the parts consistent with desired targets can be distinguished from the other parts. After this process, the image was converted to a binary one. Centroids of each target region are obtained in the binary images. After repeating such process on the images from starting point to the finishing point of the test, elongation between targets is calculated based on the centroid of each target. The tensile test were conducted on grade 60 #7(D22) and #9(D29) rebars fabricated in accordance with ASTM A615 standards. Strain results from image processing were compared to the results from a conventional strain gauge, in order to see the validity of the image processing. With the image processing, the measuring was possible in not only the initial elastic region but also the necking region of more than 0.5(50%) strain. The image processing can remove the measuring limits as long as the targets can be video recorded. It also can measure strain at various spots because the targets can easily be attached and detached. Thus it is concluded that the image processing helps overcome limits in strain measuring and will be used in various ways.

• Journal of the Korean earth science society
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• v.31 no.7
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• pp.681-690
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• 2010

Inversion result of geophysical data given as a block type was geostatistically simulated with borehole observation given as a point type and was applied to the rock classifying map. The geophysical data generally involved secondary information for the target material and were obtained for overall region. In contrast, borehole data provided direct information for the target material, but tended to be effective only for a narrow range of region and were dealt as a point type. Integrated simulation or kriging interpolation of these two different kinds of information required the covariance for point-point, point-block and block-block. Using the Bssim module included in SGeMS software, integrated result of geophysical data and borehole data were obtained. The results were then compared with the method of geostatistical inversion proposed by authors. Downscaling method used in this study showed relatively more flexible than the geostatistical inversion.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.55-55
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• 2003

The random insertion of useful gene in genome has been a common method to produce transgenic animals. This method is inefficient for induction of high levels gene expression in transgenic animals. To improve this limit, we tried to develop the system which target the gene at the specific genomic region. Thus, in our experiment, the vector system to target the human thrombopoietin (TPO) gene was developed. Targeting vector including TPO, neo and DT genes was transfrcted into bovine embryonic fibroblasts (bEF) or bovine ear skin fibroblasts (bESF). First of all, we determined concentration of the geneticin (G418) for selection of transfected cell lines. Our results showed that 1200 and 900 $\mu\textrm{g}$/ml of G418 were the most proper for selection of transfscted bEF and bESF cells. In this study, lipofectamine was used as a transfection reagent. Thus, the proper ratio of DNA:lipofectamine for transfection was also required to elevate targeting efficiency in primary mammalian cells. Our result indicates that the most proper ratios of DNA:lipofectamine were 4:2 and 1:2 in bEF and bESF cells. According to the optimized these conditions, single colonies were picked following transfection and were analyzed by PCR. More than 90% of the single colonies have TPO gene. However, there were no colonies with targeted TPO at the specific genomic region. Therefore, further experiments to select the specifically targeted colonies and to find more efficient methods such as reducing selection time and shortening a size of TPO gene are required.

• The Journal of Korean Society for Radiation Therapy
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• v.11 no.1
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• pp.100-105
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• 1999

Purpose : When the value of X,Y,Z coordination of the isocenter are reallocated from an arbitrary point using DRR (Digitally Reconstructed Radiographs) image in CT Simulation, conventional simulation is normally performed to verify the accuracy of this reallocation of the isocenter through the fluroscopy. The purpose of our experiment is to determine whether repeated test of the verification is necessary or not, and to analyze errors of reallocation with respect to the body region and the beam projection, if necessary, Material and Method : For 200 simulation patient, an arbitrary point is marked on each body and axial scaning is performed using CT, and treatment planing is done by drawing tumor and target volume on each slice. Using the planing data and the reallocated point of the isocenter, DRR image can be obtained and the final isocenter are marked on the patient's skin. In order to verify this reallocation of X,Y.Z coordination from CT simulation, We measure and evaluate the errors of these value on the fluoroscopy monitor and systematize them by classifying according to each body region (Brain, Neck and SCL, Lung, Esophagus, abdomen, Breast and Pelvis) and each beam projection {AP(PA), Supine, Prone and conformal : etc. } Conclusion : Isocenters are shifted by 3-5 mm in the case of Neck & SCL, Breast. at Abdomen, while noticeable differences are not found in other regions. Also, there are not correlations between the errors and the body regions or beam projections. However, our experiment intends to decide whether the procedure of verification is necessary on the vase of time and economy. It is regretful that we could not fully analyze the geometrical errors of DRR image and visual errors from the divergence. In conclusion, according to how much doctor consider tumor margin in drawing tumor and target volume, the meaning of analysis on the reallocation of isocenter should be reinterpreted, (which depends on the experience and capability of doctors)

• Journal of The Korean Astronomical Society
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• v.46 no.6
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• pp.203-224
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• 2013

Wide-field $JHK_s$ images obtained with the SIRIUS near-infrared camera of the IRSF 1.4m telescope are used to examine the tidal structures of the spatial stellar configuration around six metal-poor ([Fe/H]< -1.0) globular clusters located within 3 kpc from the Galactic center. The radial surface density profiles are obtained from the surface photometry of the cluster images and the star counting for the photometric data. For the star counting, candidates of cluster member stars are selected with an filtering algorithm in color-magnitude diagrams. We find that the six target clusters show tidal overdensity features in the radial surface density profiles. There is a break inside the tidal radius for each cluster, and the profile in the outer overdensity region is characterized by a power law. Two-dimensional density maps of all the clusters show distorted asymmetric stellar configurations in the outer region. In five out of the six target clusters, the overdensity features are likely to be associated with the effects of the Galaxy dynamical interaction and the cluster space motions. The observed tidal configurations of stars suggest that several metal-poor clusters in the Galactic bulge are possibly surviving remnants of mergers to build the old stellar system of the Galactic bulge.

• Proceedings of the Korean Society of Medical Physics Conference
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• 2002.09a
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• pp.146-149
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• 2002

The aim of stereotactic radiosurgery(SRS) is to deliver a high dose to a target region and a low dose to critical organ through only one or a few irradiation. To satisfy this aim, optimized irradiating conditions must be searched in the planning. Thus, many mathematical methods such as gradient method, simulated annealing and genetic algorithm had been proposed to find out the conditions automatically. There were some limitations using these methods: the long calculation time, and the difficulty of unique solution due to the different shape of tumor. In this study, optimization protocol using ideal models and data base was proposed. Proposed optimization protocol constitutes two steps. First step was a preliminary work. Some possible ideal geometry shapes, such as sphere, cylinder, cone shape or the combination, were assumed to approximate the real tumor shapes. Optimum variables such as isocenter position or collimator size, were determined so that the high dose region could be shaped to fit ideal models with the arrangement of multiple isocenter. Data base were formed with those results. Second, any shaped real targets were approximated to these models using geometry comparison. Then, optimum variables for ideal geometry were chosen from the data base predetermined, and final parameters were obtained by adjusting these data. Although the results of applying the data base to patients were not superior to the result of optimization in each case, it can be acceptable as a starting point of plan.