• Journal of Sensor Science and Technology
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• v.27 no.6
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• pp.392-396
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• 2018

Structural color has many advantages over pigment based color. In recent years, researches are being conducted to apply these advantages to applications such as wearable devices. In this study, strain sensor, a kind of wearable device, was developed using structural color. The use of structural color has the advantage of not using energy and complex measuring equipment to measure strain rate. Wrinkle structure was fabricated on the surface of Poly-dimethylsiloxane (PDMS) and used it as a sensor which color changes according to the applied strain. In addition, a transmittance-changing sensor was developed and fabricated by synthesizing additional glass nanoparticles. Furthermore, a strain sensor was developed that is largely transparent at the target strain and opaque otherwise.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.82-85
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• 2005

Doxorubicin is a highly-valuable anthracycline-family polyketide drug with a very potent anticancer activity, typically produced by a Gram-positive soil bacterium called Streptomyces peucetius. Thanks to the recent development of Streptomyces genomics-based technologies, the random mutagenesis approach for Streptomyces strain improvement has been switched toward the genomics-based technologies including the application of DNA microarray systems. In order to identify and characterize the genomics-driven potential target genes critical for doxorubincin overproduction, three different types of doxorubicin overproducing strains, a dnrI(doxorubicin-specific positive regulatory gene)-overexpressor, a doxA (gene involved in the conversion from daunorubicin to doxorubicin)-overexpressor, and a recursively-mutated industrial strain, were generated and examined their genomic transcription profiles using Streptomyces DNA microarray systems. The DNA microarray results revealed several potential target genes in S. peucetius genome, whose expressions were significantly either up- or down-regulated comparing with the wild-type strain. A systematic understanding of doxorubicin overproduction at the genomic level presented in this research should lead us a rational design of molecular genetic strain improvement strategy.

• International Journal of Oral Biology
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• v.46 no.4
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• pp.155-159
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• 2021

This study aimed to develop strain-specific polymerase chain reaction (PCR) primers to detect Fusobacterium hwasookii KCOM 1249^T, F. hwasookii KCOM 1253, F. hwasookii KCOM 1256, F. hwasookii KCOM 1258, and F. hwasookii KCOM 1268 on the basis of nucleotide sequences of a gene specific to each strain. The unique genes for each F. hwasookii strain were determined on the basis of their genome sequences using Roary. The strain-specific PCR primers based on each strain-specific gene were designed using PrimerSelect. The specificity of each PCR primer was determined using the genomic DNA of the 5 F. hwasookii strains and 25 strains of oral bacterial species. The detection limit and sensitivity of each strain-specific PCR primer pair were determined using the genomic DNA of each target strain. The results showed that the strain-specific PCR primers correspond to F. hwasookii KCOM 1249^T, F. hwasookii KCOM 1253, F. hwasookii KCOM 1258, F. hwasookii KCOM 1256/F. nucleatum subsp. polymorphum KCOM 1260, or F. hwasookii KCOM 1268/Fusobacterium sp. oral taxon 203 were developed. The detection limits of these strain-specific PCR primers ranged from 0.2 to 2 ng of genomic DNA for each target strain. The results suggest that these strain-specific PCR primers are valuable in quality control for detecting specific F. hwasookii strains.

• Archives of Pharmacal Research
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• v.23 no.6
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• pp.535-547
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• 2000

Recent findings indicate that mechanical strain (deformation) exerted by the extracellular matrix modulates activation of airway smooth muscle cells. Furthermore, cytoskeletal organization in airway smooth muscle appears to be dynamic, and subject to modulation by receptor activation and mechanical strain. Mechanosensitive modulation of crossbridge activation and cytoskeletal organization may represent intracellular feedback mechanisms that limit the shortening of airway smooth muscle during bronchoconstriction. Recent findings suggest that receptor-mediated signal transduction is the primary target of mechanosensitive modulation. Mechanical strain appears to regulate the number of functional G-proteins and/or phospholipase C enzymes in the cell membrane possibly by membrane trafficking and/or protein translocation. Dense plaques, membrane structures analogous to focal adhesions, appear to be the primary target of cytoskeletal regulation. Mechanical strain and receptor-binding appear to regulate the assembly and phosphorylation of dense plaque proteins in airway smooth muscle cells. Understanding these mechanisms may reveal new pharmacological targets for control1ing airway resistance in airway diseases.

• Proceedings of the Korean Biophysical Society Conference
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• 2002.06b
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• pp.30-30
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• 2002

The native forms of common globular proteins are in their most stable state but the native forms of plasma serpins (serine protease inhibitors) show high-energy state interactions. The high-energy state strain of a ${\alpha}$$_1$-antitrypsin, a prototype serpin, is distributed throughout the whole molecule, but the strain that regulates the function directly appears to be localized in the region where the reactive site loop is inserted during complex formation with a target protease.(omitted)

• Proceedings of the Korean Biophysical Society Conference
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• 2001.06a
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• pp.31-31
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• 2001

The native strain of serpins (serine protease inhibitors) has been recognized as a mechanism of biological regulation. Indeed, some stabilizing single residue mutations of human $\alpha$$_1$-antitrypsin, a prototype serpin, relieved local strain and caused the loss of inhibitory activity. The native strain of $\alpha$$_1$-antitrypsin is distributed throughout the whole molecule, but the strain that regulates the function directly is highly localized in the regions that appear to be mobilized during complex formation with a target protease.(omitted)

• Smart Structures and Systems
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• v.20 no.3
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• pp.385-396
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• 2017

The recent advancements in sensing technologies allow us to record measurements from target structures at multiple locations and with relatively high spatial resolution. Such measurements can be used to develop data-driven methodologies for condition assessment, control, and health monitoring of target structures. One of the state-of-the-art technologies, Fiber Optic Strain Sensors (FOSS), is developed at NASA Armstrong Flight Research Center, and is based on Fiber Bragg Grating (FBG) sensors. These strain sensors are accurate, lightweight, and can provide almost continuous strain-field measurements along the length of the fiber. The strain measurements can then be used for real-time shape-sensing and operational load-estimation of complex structural systems. While several works have demonstrated the successful implementation of FOSS on large-scale real-life aerospace structures (i.e., airplane wings), there is paucity of studies in the literature that have investigated the potential of extending the application of FOSS into civil structures (e.g., tall buildings, bridges, etc.). This work assesses the feasibility of using FOSS to predict operational loads (e.g., wind loads) on chain-like structures. A thorough investigation is performed using analytical, computational, and experimental models of a 4-story steel building test specimen, developed at the University of Southern California. This study provides guidelines on the implementation of the FOSS technology on building-like structures, addresses the associated technical challenges, and suggests potential modifications to a load-estimation algorithm, to achieve a robust methodology for predicting operational loads using strain-field measurements.

• Biomolecules & Therapeutics
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• v.29 no.2
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• pp.234-247
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• 2021

We used a heterozygous gene deletion library of fission yeasts comprising all essential and non-essential genes for a microarray screening of target genes of the antifungal terbinafine, which inhibits ergosterol synthesis via the Erg1 enzyme. We identified 14 heterozygous strains corresponding to 10 non-essential [7 ribosomal-protein (RP) coding genes, spt7, spt20, and elp2] and 4 essential genes (tif302, rpl2501, rpl31, and erg1). Expectedly, their erg1 mRNA and protein levels had decreased compared to the control strain SP286. When we studied the action mechanism of the non-essential target genes using cognate haploid deletion strains, knockout of SAGA-subunit genes caused a down-regulation in erg1 transcription compared to the control strain ED668. However, knockout of RP genes conferred no susceptibility to ergosterol-targeting antifungals. Surprisingly, the RP genes participated in the erg1 transcription as components of repressor complexes as observed in a comparison analysis of the experimental ratio of erg1 mRNA. To understand the action mechanism of the interaction between the drug and the novel essential target genes, we performed isobologram assays with terbinafine and econazole (or cycloheximide). Terbinafine susceptibility of the tif302 heterozygous strain was attributed to both decreased erg1 mRNA levels and inhibition of translation. Moreover, Tif302 was required for efficacy of both terbinafine and cycloheximide. Based on a molecular modeling analysis, terbinafine could directly bind to Tif302 in yeasts, suggesting Tif302 as a potential off-target of terbinafine. In conclusion, this genome-wide screening system can be harnessed for the identification and characterization of target genes under any condition of interest.

• Architectural research
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• v.12 no.1
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• pp.39-47
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• 2010

This paper describes a topology optimization technique which can maximize the fundamental frequency of the structures. The fundamental frequency maximization is achieved by means of the minimization of modal strain energy as an inverse problem so that the strain energy based resizing algorithm is directly used in this study. The strain energy to be minimized is therefore employed as the objective function and the initial volume of structures is used as the constraint function. Multi-frequency problem is considered by the introduction of the weight which is used to combine several target modal strain energy terms into one scalar objective function. Several numerical examples are presented to investigate the performance of the proposed topology optimization technique. From numerical tests, it is found to be that the proposed optimization technique is extremely effective to maximize the fundamental frequency of structure and can successfully consider the multi-frequency problems in the topology optimization process.

• Transactions of Materials Processing
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• v.14 no.1 s.73
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• pp.65-70
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• 2005

A phase mixture model was employed to simulate the deformation behaviour of metallic materials covering a wide grain size range from micrometer to nanometer scale. In this model a polycrystalline material is treated as a mixture of two phases: grain interior phase whose plastic deformation is governed by dislocation and diffusion mechanisms and grain boundary 'phase' whose plastic flow is controlled by a boundary diffusion mechanism. The main target of this study was the effect of grain size on stress and its strain rate sensitivity as well as on the strain hardening. Conventional Hall-Petch behaviour in coarse grained materials at high strain rates governed by the dislocation glide mechanism was shown to be replaced with inverse Hall-Petch behaviour in ultrafine grained materials at low strain rates, when both phases deform predominantly by diffusion controlled mechanisms. The model predictions are illustrated by examples from literature.