• Biomedical Science Letters
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• v.17 no.1
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• pp.1-5
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• 2011

Previously, we have identified 14 putative downstream target genes of Hoxc8 homeoprotein in F9 murine embryonic teratocarcinoma cells through proteomics analysis. Among those, we tested a possibility of a DNA-k type molecular chaperone, Grp78, as a direct downstream target of Hoxc8, by cloning a 2.4 kb upstream region of murine Grp78 into a reporter plasmid and by testing if Hoxc8 can regulate its expression. We observed that Hoxc8 proteins could transactivate the reporter gene, which was affected by small interference RNAs (siRNAs) against to Hoxc8, suggesting that Grp78 is a novel downstream target of Hoxc8 in vivo.

• Korean Journal of Heritage: History & Science
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• v.40
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• pp.137-167
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• 2007

This paper is aimed to study the late Neolithic Age in North Korea in order to closely examine a transition process into the Bronze Age in the Korean peninsula. Thus, the pottery with lightning design was selected as target data. Since the pottery with lightning design is fundamental data that North Korea's archeologists have used for chronological recordings of the late Neolithic Age in the northwest region, the parallel relationship between the eastern and the western region was established with comparison of pottery with lightning design in the northeastern region. The examination focuses on data that cover the target region of the pottery with lightning design of the adjacent Southern Primorskii region including the counterpart of the northwestern and the northeastern region. As a result, some attribute analyses revealed that the pottery with lightning design was affected by the counterpart of the northwestern region near the Yalu River. Prior to genealogical and chronological recordings of the pottery with lightning design in the northeastern region, the pottery with lightning design in the northwestern region was recorded chronologically in order to examine its specific development process. Consequently, in the second period of the Neolithic Age, the pottery with lightning design in the northwestern region was assumed to have an impact on the counterpart in the northeastern region. The classification of the pottery with lightning design in the northeastern region shall be based on 4-period development. According to this classification, the pottery, which was found in the Tumen river basin, was thought to belong to the first period. The pottery went through genealogy differentiation in the second period and when entering into the third period, the pottery spread to inland of south Primorskii. The pottery was assumed to exist in the southern Primorskii region until the tip end (the fourth period) of the Neolithic Age. It is assumed that considering the fact that climate change led to the agriculture movement, Zaisanovka culture, i.e. the late Ne Neolithic Age, moved to the southern Primorskii region along the Tumen River basin.

• BMB Reports
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• v.29 no.2
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• pp.175-179
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• 1996

Viral surface proteins are known to play an essential role in attachment of the virus particle to the host cell membrane. In case of the hepatitis B virus (HBV) several reports have described potential receptors on the target cell side, but no definite receptor protein has been isolated yet. As for the viral side, it has been suggested that the preS region of the envelope protein, especially the preS1 region, is involved in binding of HBV to the host cell. In this study, preS1 region was recombinantly expressed in the form of a maltose binding protein (MBP) fusion protein and used to identify and visualize the expression of putative HBV receptor(s) on the host cell. Using laser scanned confocal microscopy and by FACS analysis, MBP-preS1 proteins were shown to bind to the human hepatoma cell line HepG2 in a receptor-ligand specific manner. The binding kinetic of MBP-preS1 to its cellular receptor was shown to be temperature and time dependent. In cells permeabilized with Triton X-100 and treated with the fusion protein, a specific staining of the nuclear membrane could be observed. To determine the precise location of the receptor binding site within the preS1 region, several short overlapping peptides from this region were synthesized and used in a competition assay. In this way the receptor binding epitope in preS1 was revealed to be amino acid residues 27 to 51, which is in agreement with previous reports. These results confirm the significance of the preS1 region in virus attachment in general, and suggest an internalization pathway mediated by direct attachment of the viral particle to the target cell membrane.

• Journal of Korea Multimedia Society
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• v.19 no.4
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• pp.725-735
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• 2016

High-resolution satellite images are used in the fields of mapping, natural disaster forecasting, agriculture, ocean-based industries, infrastructure, and environment, and there is a progressive increase in the development and demand for the applications of high-resolution satellite images. Users of the satellite images desire accurate quality of the provided satellite images. Moreover, the distinguishability of each image captured by an actual satellite varies according to the atmospheric environment and solar angle at the captured region, the satellite velocity and capture angle, and the system noise. Hence , NIIRS must be measured for all captured images. There is a significant deficiency in professional human resources and time resources available to measure the NIIRS of few hundred images that are transmitted daily. Currently, NIIRS is measured every few months or even few years to assess the aging of the satellite as well as to verify and calibrate it [3]. Therefore, we develop an algorithm that can measure the national image interpretability rating scales (NIIRS) of a typical satellite image rather than an artificial target satellite image, in order to automatically assess its quality. In this study, the criteria for automatic edge region extraction are derived based on the previous works on manual edge region extraction [4][5], and consequently, we propose an algorithm that can extract the edge region. Moreover, RER and H are calculated from the extracted edge region for automatic edge region extraction. The average NIIRS value was measured to be 3.6342±0.15321 (2 standard deviations) from the automatic measurement experiment on a typical satellite image, which is similar to the result extracted from the artificial target.

• Bulletin of the Korean Chemical Society
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• v.20 no.9
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• pp.1078-1084
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• 1999

A synthetic cecropin A(1-13)-melittin(1-13) [CA-ME] hybrid peptide was known to be an antimicrobial peptide having strong antibacterial, antifungal and antitumor activity with minimal cytotoxic effect against human erythrocyte. Analogues were synthesized to investigate the influences of the flexible hinge region of CA-ME on the antibiotic activity. Antibiotic activity of the peptides was measured by the growth inhibition against bac-terial, fungal and tumor cells and vesicle-aggregating or disrupting activity. The deletion of Gln-Gly-Ile (P1) or Gly-Gln-Gly-Ile-Gly (P3) from CA-ME brought about a significant decrease on the antibiotic activities. In contrast, Gly-Ile-Gly deletion (P2) from CA-ME or Pro insertion (P5) instead of Gly-Gln-Gly-Ile-Gly of CA-ME retained antibiotic activity. This result indicated that the flexible hinge or β-bend structure provided by Gly-Gln-Gly-Ile-Gly, Gln-Gly, or Pro in the central region of the peptides is requisite for its effective antibiotic activity and may facilitate easily the hydrophobic C-terminal region of the peptide to penetrate the lipid bilayers of the target cell membrane. In contrast, P4 and P6 with Gly-Gln-Gly-Pro-Gly or Gly-Gln-Pro in the central region of the peptide caused a drastic reduction on the antibiotic activities. This result suggested that the con-secutive β-bend structure provided by Gly-Gln-Gly-Pro-Gly or Gly-Gln-Pro in the central hinge region of the peptide seems to interrupt the ion channel/pore formation on the target cell membranes.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.39 no.7
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• pp.38-45
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• 2002

This paper derives the electric field integral equation to calculate scattering from arbitrary large target above and radiating of an electric line source within a lossy ground. Sommerfeld’s type integral requires a lot of time to calculate and has some difficulties and limitations for an analysis region. But SDP (steepest descent path) integration gives fast calculation of the integral, and the result shows that SDP integration has the validity for all over the analysis region with fast evaluation. Moment method with SDP integration is used to calculate the scattering of an arbitrary large conducting target and the results are compared with that of the numerical integration with Gaussian quadrature rule and GPOF (generalized pencil of function) method.

• The Journal of Korea Robotics Society
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• v.12 no.1
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• pp.33-41
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• 2017

We present a region-based approach for accurate pose estimation of small mechanical components. Our algorithm consists of two key phases: Multi-view object co-segmentation and pose estimation. In the first phase, we explain an automatic method to extract binary masks of a target object captured from multiple viewpoints. For initialization, we assume the target object is bounded by the convex volume of interest defined by a few user inputs. The co-segmented target object shares the same geometric representation in space, and has distinctive color models from those of the backgrounds. In the second phase, we retrieve a 3D model instance with correct upright orientation, and estimate a relative pose of the object observed from images. Our energy function, combining region and boundary terms for the proposed measures, maximizes the overlapping regions and boundaries between the multi-view co-segmentations and projected masks of the reference model. Based on high-quality co-segmentations consistent across all different viewpoints, our final results are accurate model indices and pose parameters of the extracted object. We demonstrate the effectiveness of the proposed method using various examples.

• Journal of Korean Society of Rural Planning
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• v.23 no.4
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• pp.15-26
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• 2017

In analyzing the effect of the central place improvement projects, this study classifies subject residents into two groups of the residents of the project target regions and those of surrounding villages, and conducts verification. As a result of analysis, Variety of cultural programs is the only factor that positively affects the satisfaction level of the residents of the project target region, and it has been analyzed at the significance level of 10% that a one-unit increase in securing rest areas lowers the satisfaction level of the residents of surrounding villages. This shows that the effect of the central place improvement projects currently promoted is at a low level, and there is also a risk of conflict between the residents in the target region and those in surrounding villages. Although there is no statistical significance, analysis shows that a method to provide the residents in surrounding villages with the project-related information and to guarantee their participation in the project enhances the satisfaction level of the residents of surrounding villages. Therefore, institutional improvements reflecting this need to be made.

• Journal of Information Processing Systems
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• v.13 no.5
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• pp.1183-1202
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• 2017

Image restoration has been carried out by texture synthesis mostly for large regions and inpainting algorithms for small cracks in images. In this paper, we propose a new approach that allows for the simultaneous fill-in of different structures and textures by processing in a wavelet domain. A combination of structure inpainting and patch-based texture synthesis is carried out, which is known as patch-based inpainting, for filling and updating the target region. The wavelet transform is used for its very good multiresolution capabilities. The proposed algorithm uses the wavelet domain subbands to resolve the structure and texture components in smooth approximation and high frequency structural details. The subbands are processed separately by the prioritized patch-based inpainting with isophote energy driven texture synthesis at the core. The algorithm automatically estimates the wavelet coefficients of the target regions of various subbands using optimized patches from the surrounding DWT coefficients. The suggested performance improvement drastically improves execution speed over the existing algorithm. The proposed patch optimization strategy improves the quality of the fill. The fill-in is done with higher priority to structures and isophotes arriving at target boundaries. The effectiveness of the algorithm is demonstrated with natural and textured images with varying textural complexions.

• Smart Structures and Systems
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• v.32 no.1
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• pp.49-59
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• 2023

A bridge transportation network supplies products from various source nodes to destination nodes through bridge structures in a target region. However, recent frequent earthquakes have caused damage to bridge structures, resulting in extreme direct damage to the target area as well as indirect damage to other lifeline structures. Therefore, in this study, a surrogate model-based comprehensive framework to estimate the seismic resilience of bridge transportation networks is proposed. For this purpose, total system travel time (TSTT) is introduced for accurate performance indicator of the bridge transportation network, and an artificial neural network (ANN)-based surrogate model is constructed to reduce traffic analysis time for high-dimensional TSTT computation. The proposed framework includes procedures for constructing an ANN-based surrogate model to accelerate network performance computation, as well as conventional procedures such as direct Monte Carlo simulation (MCS) calculation and bridge restoration calculation. To demonstrate the proposed framework, Pohang bridge transportation network is reconstructed based on geographic information system (GIS) data, and an ANN model is constructed with the damage states of the transportation network and TSTT using the representative earthquake epicenter in the target area. For obtaining the seismic resilience curve of the Pohang region, five epicenters are considered, with earthquake magnitudes 6.0 to 8.0, and the direct and indirect damages of the bridge transportation network are evaluated. Thus, it is concluded that the proposed surrogate model-based framework can efficiently evaluate the seismic resilience of a high-dimensional bridge transportation network, and also it can be used for decision-making to minimize damage.