• BMB Reports
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• v.34 no.1
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• pp.73-79
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• 2001

Transcriptional activation domains are known to be inherently "unstructured" with no tertiary structure. A recent NMR study, however, has shown that the transactivation domain in human p53 is populated with an amphipathic helix and two nascent turns. This suggests that the presence of such local secondary structures within the overall "unstructured" structural framework is a general feature of acidic transactivation domains. These pre-existing local structures in p53, formed selectively by positional conserved hydrophobic residues that are known to be critical for transcriptional activity, thus appear to constitute the specific structural motifs that regulate recognition of the p53 transactivation domain by target proteins. Here, we report the results of a NMR structural comparison between the native human p53 transactivation domain and an inactive mutant (22L,23W$\rightarrow$22R,23S). Results show that the mutant has an identical overall structural topology as the native protein, to the extent that the amphipathic helix formed by the residues 18T 26L within the native p53 transactivating domain is preserved in the double mutant. Therefore, the lack of transcriptional activity in the double mutant should be ascribed to the disruption of the essential hydrophobic contacts between the p53 transactivation domain and target proteins due to the (22L,23W$\rightarrow$22R,23S) mutation.

• Journal of the Korean School Mathematics Society
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• v.15 no.3
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• pp.535-563
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• 2012

This study was conducted to confirm the necessity of analogical thinking and to empirically verify the effectiveness of analogical reasoning through the visual representation by analyzing the factors of problem solving depending on analogical conditions. Four conditions (a visual representation mapping condition, a conceptual mapping condition, a retrieval hint condition and no hint condition) were set up for the above purpose and 80 twelfth-grade students from C high-School in Cheong-Ju, Chung-Buk participated in the present study as subjects. They solved the same mathematical problem about sequence of complex numbers in their differed process requirements for analogical transfer. The problem solving rates for each condition were analyzed by Chi-square analysis using SPSS 12.0 program. The results of this study indicate that retrieval of base knowledge is restricted when participants do not use analogy intentionally in problem solving and the mapping of the base and target concepts through the visual representation would be closely related to successful analogical transfer. As the results of this study offer, analogical thinking is necessary while solving mathematical problems and it supports empirically the conclusion that recognition of the relational similarity between base and target concepts by the aid of visual representation is closely associated with successful problem solving.

• Korean Journal of Cognitive Science
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• v.25 no.2
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• pp.91-107
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• 2014

The syllable frequency effect is defined as the inhibitory effect that words starting with high frequency syllable generate a longer lexical decision latency and a larger error rate than words starting with low frequency syllable do. Researchers agree that the reason of the inhibitory effect is the interference from syllable neighbors sharing a target's first syllable at the lexical level and the degree of the interference effect correlates with the number of syllable neighbors or stronger syllable neighbors which have a higher word frequency. However, although the syllable frequency can be classified as the syllable type and token frequency, previous studies in visual word recognition have used the syllable frequency without the classification. Recently Conrad, Carreiras, & Jacobs (2008) demonstrated that the syllable type frequency might reflect a sub-lexical processing level including matching from letters to syllables and the syllable token frequency might reflect competitions between a target and higher frequency words of syllable neighbors in the whole word lexical processing level. Therefore, the present study investigated their proposals using word naming tasks. Generally word naming tasks are more sensitive to sub-lexical processing. Thus, the present study expected a facilitative effect of high syllable type frequency and a null effect of high syllable token frequency. In Experiment 1, words starting with high syllable type frequency generated a faster naming latency than words starting with low syllable type frequency with holding syllable token frequency of them. In Experiment 2, high syllable token frequency also created a shorter naming time than low syllable token frequency with holding their syllable type frequency. For that reason, we rejected the propose of Conrad et al. and suggested that both type and token syllable frequency could relate to the sub-lexical processing.

• Communications of Mathematical Education
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• v.24 no.2
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• pp.385-413
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• 2010

The purpose of this study is to set appropriate targets for school-year levels and types of mathematical communication. First, I classify mathematical communication into four types as Discourse, Representation, Operation and Complex and refer to them collectively as the 'D.R.O.C pattern'. I have listed achievement factors based on the D.R.O.C pattern hearing opinions from specialists to set a target, then set a final target after a 2nd survey with specialists and teachers. I have set targets for mathematical communication in elementary schools suitable to its status and students' levels in our country. In NCTM(2000), standards of communication were presented only from kindergarten to 12th grade students, and, for four separate grade bands(prekindergarten through grade 2, grades 3-5, grades 6-8, grades 9-12), they presented characteristics of the same age group through analysis of classes where communication was active and the stated roles of teachers were suitable to the characteristics of each school year. In this study, in order to make the findings accessible to teachers in the field, I have classified types into Discourse, Representation, Operation and Complex (D.R.O.C Pattern) according to method of delivery, and presented achievement factors in detail for low, middle and high grades within each type. Though it may be premature to set firm targets and achievement factors for each school year group, we hope to raise the possibility of applying them in the field by presenting targets and achievement factors in detail for mathematical communication.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.5
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• pp.1891-1896
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• 2010

This study aims at illuminating the state of car seat use and relevant factors and providing basic data to enhance the consciousness of children's safety with the target group of parents who have 5~6 year old kindergarten pupils in a specific city. The target group to study is 189 fathers and mothers who have 5~6 year old kindergarten pupils in five different kindergartens in a city. Structured questionnaires are used and the major research results are the followings: The use rate of children's car seat is the higher in the lower age (p=0.128), boys (p=0.474), mothers below the age of 30s(p=0.294). The use of children's car seat is significant in the mothers who graduated high school or lower (p=0.009), and fathers(p=0.010) who are working in offices or service occupations. The study on the recognition rate of the free lease of children's car seat conducted by Korea Children Safety Foundation shows parents who earned 3 million won or lower per month(p=0.009) significantly, and the major significant factors affecting the use of children's car seat are existence or nonexistence of car seat education and kinds of parent's occupation.

• Journal of the Korea Society of Computer and Information
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• v.18 no.10
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• pp.109-119
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• 2013

An unmanned ground vehicle(UGV) today plays a significant role in both civilian and military areas. Predominantly these systems are used to replace humans in hazardous situations. To take unmanned ground vehicles systems to the next level and increase their capabilities and the range of missions they are able to perform in the combat field, new technologies are needed in the area of command and control. For this reason, we present war field simulator based on information fusion technology to efficiently control UGV. In this paper, we present the war field simulator which is made of critical components, that is, simulation controller, virtual image viewer, and remote control device to efficiently control UGV in the future combat fields. In our information fusion technology, improved methods of target detection, recognition, and location are proposed. In addition, time reduction method of target detection is also proposed. In the consequence of the operation test, we expect that our war field simulator based on information fusion technology plays an important role in the future military operation significantly.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.23 no.2
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• pp.69-78
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• 2024

Recently, the use of CCTV to prevent crowd accidents has been promoted, but research is needed to compensate for the spatial limitations of CCTV. In this study, pedestrian density was measured using drone footage, and based on a review of existing literature, a threshold of 6.7 people/m² was selected as the cutoff risk level for crowd accidents. In addition, we conducted a preliminary study to determine drone parameters and found that the pedestrian recognition rate was high at a drone altitude of 20 meters and an angle of 60°. Based on a previous study, we selected a target area with a high concentration of pedestrians and measured pedestrian density, which was found to be 0.27~0.30 per m². The study shows it is possible to measure risk levels by determining pedestrian densities in target areas using drone images. We believe drone surveillance will be utilized for crowd safety management in the near future.

• BMB Reports
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• v.40 no.5
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• pp.783-790
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• 2007

Receptor binding plays an important role in determining host specificity of the Bacillus thuringiensis Cry $\delta$-endotoxins. Mutations in domains II and III have suggested the participation of certain residues in receptor recognition and insect specificity. In the present study, we expressed the cloned domain II-III fragment of Cry4Ba and examined its binding characteristics to mosquito-larval midgut proteins. The 43-kDa Cry4Ba-domain II-III protein over-expressed in Escherichia coli as inclusion bodies was only soluble when carbonate buffer, pH 10.0 was supplemented with 4M urea. After renaturation via stepwise dialysis and subsequent purification, the refolded domain II-III protein, which specifically reacts with anti Cry4Ba-domain III monoclonal antibody, predominantly exists as a $\beta$-sheet structure determined by circular dichroism spectroscopy. In vitro binding analysis to both histological midgut tissue sections and brush border membrane proteins prepared from susceptible Aedes aegypti mosquito-larvae revealed that the isolated Cry4Ba-domain II-III protein showed binding functionality comparable to the 65-kDa full-length active toxin. Altogether, the data present the 43-kDa Cry4Ba fragment comprising domains II and III that was produced in isolation was able to retain its receptor-binding characteristics to the target larval midgut proteins.

• Journal of the Korean Society of Clothing and Textiles
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• v.32 no.4
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• pp.669-680
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• 2008

Korea's national image can be delivered through cultural products, and cultural products can finally contribute to the affirmative attitude and recognition towards Korea. In an empirical study, a questionnaire was developed based on literature review and focus group interviews with foreigners who visit Korea were executed. Subjects of this study were foreign visitors from US, China, and Japan. Finally, a total of 247 copies were used for analyses. Descriptive analyses, factor analysis, regression analysis, ANOVA, Duncan test, and paired sample t-test were conducted for data analysis, Results are as follows. Firstly, as a result of factor analysis, I found that there were seven factors to form the image of Korea; culture/art, openness, lechnology/quality, food culture, passionate narcissism, conservativeness, and pursuit of quality of life. Secondly, Several image factors were found to influence positively or negatively on their attitude toward fashion cultural products. Especially, culture/art factor was found to influence positively to Chinese and Japanese and lechnology/quality factor was found to influence positively to Japanese and American. Meanwhile, conservativeness factor was found to influence negatively to Chinese. This study will be of help to practitioners of the fashion cultural product industry for building marketing strategies whose target market is foreign visitors from different cultural backgrounds.

• Journal of Korean Society of Rural Planning
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• v.14 no.2
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• pp.33-42
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• 2008

Recently, rural leisure by urban dwellers has been recognized as a catalyst for regional development or restoration. The federal government implemented several regional planning policies attempting to address this phenomenon in the late nineties. However, most of these policies focused on supply-centered planning, which caused a diverse set of problems relating to the sustainability and economic validity of the planning. Instead, this study focuses on the re-visitation one of the demand variables, with the intention of analyzing the character of rural leisure demand and its re-visitation choice attributes. For these reasons, this study investigates the character of current rural leisure demand, and develops functional equations about urban people who visit rural areas, using logit model. The results of this study can be summarized as follow: Urban people are visiting rural areas from nearby cities or metropolitan areas that are within 3 hours driving distance, and over 80% of rural visitors are one-day visitors. Therefore, most of their visitation activities should be understood as rural leisure, including rural tourism. In addition, the likelihood of the rural tourist to make a return visit is influenced by their transportation means, product purchasing, staying period and travel time. The results of such rural leisure demand can provide information for regional planning strategies, such as selection of target market, type of market segments, improvement of recognition level, activity program price, facility and space planning, produce sale and connection with tour site.