• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.6
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• pp.747-752
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• 2011

The parrotfish Oplegnathus fasciatus is a sub-tropical species that is difficult to culture during the winter in South Korea. As a result, a recirculating aquaculture system (RAS) was developed to rear parrotfish. This study investigated the effects of stocking density and dissolved oxygen (DO) concentration on the growth and hematology of the parrotfish in the RAS. The experimental stocking densities were 5 (SD05), 10 (SD10), 15 (SD15), and 20 kg/m3 (SD20) total body weight to tank water volume. As the stocking density increased, the mean weight gain, feed efficiency, and specific growth rate tended to decrease. However, SD10 and SD15 did not differ statistically from SD05 in feed efficiency (P>0.05). Although better growth was achieved in SD05, SD10 and SD15 appeared to be acceptable, practically and economically, in terms of feed efficiency. The experimental DO concentration ranges were 3-4 (DO3), 5-6 (DO5), and 7-8 mg/L (DO7). The mean weight gain and survival tended to increase with the DO concentration, but there were no differences among treatments (P>0.05). Although the feed efficiency did not differ among the treatments, the specific growth rates and daily feed intake increased with the DO concentration and were the highest in DO7 (P<0.05). The cortisol concentration was the highest in DO3 (P<0.05), while there was no difference between DO5 and DO7 (P>0.05).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.5
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• pp.465-472
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• 2001

The effects of water level reduction in rearing tank and fish transference during fish selection process on the stress response (hematological factors, cortisol, glucose, lactic acid and osmolality) of tank-reared olive flounder Paralichthys olivaceus of large (FL), small (FS) and Japanese croaker, Nibea japonica (JC) were examined in running seawater culture system. The water level of rearing unit was lowered from 33 cm to 8 cm in the course of 2 minutes in the water level reduction experiment. The fish were removed from rearing tank (12 ton) to 450 L tank in 30 seconds after capture in the fish transference experiment, In water level reduction, the hematocrit of FL was significantly increased from $14.6\%$ at beginning to $23.5\%$ after 10 hours, However, it decreased to the value of beginning after 46 hours. Plasma cortisol concentration of FL was the highest concentration (13.7 ng/mL) after 22 hours, but it decreased to 4.0 ng/mL at the end of experiment. Cortisol concentration of FS did not show any significant difference during the experiment. The cortisol concentration of JC were significantly higher at 4 hours (282.3 ng/mL) and 22 hours (350.5 ng/mL), Glucose concentration of JC was the highest (138.0 mg/dL) at 22 hours. Lactic acid concentration was not different between experimental groups. In the fish transference experiment, red blood cell of FL was increased from $1.9\times10^6\;cell/{\mu}\;L\;to\;4.2\times10^6\;cell/{\mu}L$ in 24 hours. Blood hemoglobin of JC were significantly elevated in 24 hours. At 1 hour after transference, plasma cortisol concentrations in both fish species were increased to 95.3 ng/mL in FL and 175.5 ng/mL in JC. Glucose concentration of JC was increased to 132.5 mg/dL at 1 hour, 129.5 mg/dL at 3 hours after transference.

• Journal of Korean Society on Water Environment
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• v.20 no.5
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• pp.506-511
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• 2004

Maintaining dissolved oxygen (DO) at sufficiently low concentration in the aeration tank at a wastewater treatment plant (WWTP) is essential for reduction of the costs of operation and maintenance. On the other hand, the low DO level may result in adverse effect on the integrity of the activated sludge, A typical and disastrous outcome frequently experienced is the outgrowth of filamentous microorganisms, which is called as filamentous bulking, In addition to the traditional methods such as sludge settleability and microscopic observation of the culture, molecular techniques including polymerase chain reaction (PCR) amplification followed by 16S rRNA sequencing were applied to identify filamentous bacteria present in bulking sludge under a condition of low DO concentration, Two morphologically distinct groups, presumably consisting of Sphaerofilus nafans, and Eikelboom Type 1701 or Type 1851, were identified through microscopic observation. They were further confirmed by subsequent 16S rRNA sequencing. Dominant filamentous bacteria identified by the molecular techniques were consisted of three major groups. Sequences of partial 16S rRNA cloned showed that the filamentous bulking organisms were closely related to Eikelboom Type 021N and Eikelboom Type 1701, and Sphaerotilus natans, respectively. Molecular methods were found to possess a strong potential of direct examination of the microbial community of an activated sludge system.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.1
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• pp.72-79
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• 2006

Kojic acid production by Aspergillus flavus strain S44-1 using sucrose as a carbon source was carried out in a 250-mL shake flask and a 2-L stirred tank fermenter. For comparison, production of kojic acid using glucose, fructose and its mixture was also carried out. Kojic acid production in shake flask fermentation was 25.8 g/L using glucose as the sole carbon source, 23.6 g/L with sucrose, and 6.4 g/L from fructose. Reduced kojic acid production (13.5 g/L) was observed when a combination of glucose and fructose was used as a carbon source. The highest production of kojic acid (40.2 g/L) was obtained from 150 g/L sucrose in a 2 L fermenter, while the lowest kojic acid production (10.3 g/L) was seen in fermentation using fructose as the sole carbon source. The experimental data from batch fermentation and resuspended cell system was analysed in order to form the basis for a kinetic model of the process. An unstructured model based on logistic and Luedeking-Piret equations was found suitable to describe the growth, substrate consumption, and efficiency of kojic acid production by A. flavus in batch fermentation using sucrose. From this model, it was found that kojic acid production by A. flavus was not a growth-associated process. Fermentation without pH control (from an initial culture pH of 3.0) showed higher kojic acid production than single-phase pH-controlled fermentation (pH 2.5, 2.75, and 3.0).

• Journal of Marine Life Science
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• v.4 no.2
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• pp.86-90
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• 2019

This study aimed to compare the disinfection efficiencies of the ultraviolet and plasma systems, the two systems designed and commercialized to disinfect water in aquaculture, by putting each in a 100 ℓ water tank and concentrating 1.0 ℓ of treated water to check the changes in the number of bacteria in the samples. Each system was operated for 6 hours to culture the typical seawater bacteria in the Marine agar, Thiosulfate citrate bile salts sucrose agar and Salmonella Shigella agar media, respectively, to check the number of bacteria in the media, and the changes in the number of Edwardsiella piscicida in the treated water were checked after the artificial inoculation of E. piscicida in the disinfected seawater. As a result, the two disinfection systems showed the almost similar levels of bacterial reduction efficiency between 99.5% and 99.9%. However, the result of this study showed that, with 100 ℓ of water treated for the same length of time using the two systems, the plasma system turned out to disinfect bacteria in a shorter period of time than the UV system. However, as the changes in the number of bacteria were checked for a short length of time (6 hours) in this study, it was judged that, considering the actual aquaculture environment in which the quality of water significantly changes with feed residues, excretions and coastal contamination, etc., and a lot of biofilms and organic matter exist, the plasma system would be more efficient than the UV system as the former is capable of continuously maintaining a certain level of efficiency than the latter that is limited in terms of efficiency depending on the level of turbidity and the existence of organic matter.

• Journal of the Korean Society of Marine Environment & Safety
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• v.22 no.5
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• pp.483-489
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• 2016

The type approval test for USCG Phase II must be satisfied such that living natural biota occupy more than 75 % of whole biota in a test tank. Thus, we harvested a community of natural organisms using a net at Masan Bay (eutrophic) and Jangmok Bay (mesotrophic) during winter season to meet this guideline. Furthermore, cell viability was measured to determine the mortality rate. Based on the organism concentration volume (1 ton) at Masan and Jangmok Bay, abundance of ${\geq}10$ and $<50{\mu}m$ sized organisms was observed to be $4.7{\times}10^4cells\;mL^{-1}$and $0.8{\times}10^4cells\;mL^{-1}$, and their survival rates were 90.4 % and 88.0 %, respectively. In particular, chain-forming small diatoms such as Skeletonema costatum-like species were abundant at Jangmok Bay, while small flagellate ($<10{\mu}m$) and non chain-forming large dinoflagellates, such as Akashiwo sanguinea and Heterocapsa triquetra, were abundant at Masan Bay. Due to the size-difference of the dominant species, concentration efficiency was higher at Jangmok Bay than at Masan Bay. The mortality rate in samples treated by Ballast Water Treatment System (BWMS) (Day 0) was a little lower for samples from Jangmok Bay than from Masan Bay, with values of 90.4% and 93%, respectively. After 5 days, the mortality rates in control and treatment group were found to be 6.7% and >99%, respectively. Consequently, the phytoplankton concentration method alone did not easily satisfy the type approval standards of USCG Phase II ($>1.0{\times}10^3cells\;mL^{-1}$ in 500-ton tank) during winter season, and alternative options such as mass culture and/or harvesting system using natural phytoplankton communities may be helpful in meeting USCG Phase II biological criteria.

• Korean Journal of Environmental Biology
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• v.36 no.1
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• pp.90-97
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• 2018

This study assessed the effect of ozone to control pathogenic bacteria in inlet water flowing to flounder farms, establishing operational parameters of ozonation at seawater conditions. Hydraulic retention time in a reaction pipeline after ozonation was fixed at 3 minutes in a flow through system. Concentrations of total residual oxidant (TRO) by ozonation were measured according to different ozonation intensities. The oxidant reduction potential (ORP), which is indirect but enables real-time measurement, was measured in relation to TRO values. TRO values were $0.01{\pm}0.01mg\;L^{-1}$ at an ORP range of 320-410 mV, $0.07{\pm}0.02mg\;L^{-1}$ at 600 mV, and $0.16{\pm}0.03mg\;L^{-1}$ at 700 mV. A heterotrophic marine bacteria colony was reduced by 80.6-97.9%, showing the suppression effect of ozonation on total bacteria in inlet water. At an ORP range of 400-500 mV, colonies of heterotrophic marine bacteria, Vibrio spp., and gram negative bacteria were significantly reduced in outlet water from a culture tank with ongrowing flounder (230 g) at a stocking density of $8kg\;m^{-2}$. Especially, Vibrio spp. and gram negative bacteria were seldom found at 400-500 mV. The daily feeding rate was from over 0.7% to total body weight at 300-500 mV, showing better performance than that in the control. The pathogenic bacteria entering the flounder farm were effectively removed when the ORP range to 400 mV or less.

• The Korean Journal of Malacology
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• v.28 no.4
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• pp.313-319
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• 2012

Mass production method on artificial seedling production of hard clam Meretrix petechialis was developed indoor culture system. Spawning of adult clam (SL $65.8{\pm}8.4mm$) was induced using the combined method of air exposure and water temperature raising. The fertilized eggs were developed to D-shaped larvae after 17.7 hours at $27^{\circ}C$ and hatching rate was 6.1%. Shell length (SL) of D-shaped larvae was measured to be $131.4{\pm}2.6{\mu}m$ and thereafter the larvae grew to the settled spats with SL $190.2{\pm}7.5{\mu}m$ in 4 days. Estimated survival rate of settled spats was 48.1%. Spat collection on 130,000 spats with SL $0.19{\pm}0.01mm$ performed conducted by sand bottom circulation filtering method. Collected spats grew up to $3.1{\pm}0.8mm$ in 46 days, $6.6{\pm}1.8mm$ in 87 days, and $10.5{\pm}0.9mm$ in 114 days. The relative growth between SL and shell height (SH) was calculated to be SH = 0.8501SL + 0.0196 ($R^2=0.9987$) during the whole spat period. During spats rearing, they were suffered from one time of mass mortality at SL 3.1 mm, but 51,000 spats were finally survived with the rate of 39.2% at 114 days of spat rearing in indoor tank system.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.6
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• pp.581-585
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• 1986

A growth experiment of the Israeli strain of common carp (Cyprinus carpio) under varying dissolved oxygen levels in the recirculating water system was conducted at the Fish Culture Experiment Station of the National Fisheries University of Pusan from August 28, 1985 to September 17, 1985. Five tanks with a capacity of $5m^3$ of water each were used under the same condition of water parameters except for dissolved oxygen levels which were designed to maintain at 2.0, 2.5, 3.0, 3.5 and 4.0 mg/l ranges. The weight of fish in the beginning was about 300g and each tank was stocked with 200kg of fish. DO level of 3.5mg/l was found to be the best level with a feed coefficient of 1.57 and a daily growth rate of $1.411\%$ whereas 4.mg/l showed a slightly decreased performance of 1.63 and $1.365\%$ respectively. The amounts of feed consumed in 3.5 and 4.0mg/l DO levels were almost the same. Below 3.0mg/l DO levels the growth rate markedly decreased. Furthermore, in 2.0 and 2.5 mg/l groups, the fish did not accept feed vigorously and after feeding the fish usually concentrated around the inflow point showing oxygen deficiency response, The experiment indicates that the DO range of 3.5 to 4.0mg/l is the optimum level for the best growth at $27.5^{\circ}C$. DO concentration above these levels is considered a waste of energy resulting in uneconomical performance, and on the other hand, below these levels, the carp certainly shows a poor growth performance.

• Korean Journal of Environment and Ecology
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• v.33 no.5
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• pp.596-604
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• 2019

This study conducted a sediment culture experiment to investigate the effects of sediment oxygen demand (SOD) and environmental factors on sediment and water quality. We installed a leaching tank in the laboratory, cultured it for 20 days, and analyzed the relationship between P and Fe in the sediment. As a result, the dissolved oxygen of the water layer decreased with time, while the oxidation-reduction potential of the sediment progressed in the negative direction to form an anaerobic reducing environment. The SOD was measured to be 0.05 mg/g at the initial stage of cultivation and increased to 0.09 mg/g on the 20th day, indicating the tendency of increasing consumption of oxygen by the sediment. The change is likely to have caused by oxygen consumption from biological-SOD, which is the decomposition of organic matter accumulated on the sediment surface due to the increase of chl-a, and chemical-SOD consumed when the metal-reducing product produced by the reduction reaction is reoxidized. The correlation between SOD and causality for sediment-extracted sediments was positive for Ex-P and Org-P and negative for Fe-P. The analysis of the microbial community in the sediment on the 20th day showed that anaerobic iron-reducing bacteria (FeRB) were the dominant species. Therefore, when the phosphate bonded to the iron oxide is separated by the reduction reaction, the phosphate is eluted into the water to increase the primary productivity. The reduced substance is reoxidized and contributes to the oxygen consumption of the sediment. The results of this study would be useful as the reference information to improve oxygen resin.