• 설비공학논문집
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• 제17권1호
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• pp.39-46
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• 2005

The present study concerns an experimental study of a R-22 heat pump system consisted of liquid and liquid heat exchangers. The test was performed for various systems of a single-, tandem-, and two stage-cycle at the same environmental conditions of temperature. Various experiments of the heat pump system were peformed to compare the heating capacity and COP, when the outdoor temperature is near $-15^{\circ}C$ and the indoor temperature is $20^{\circ}C.$ As the results of the present study, the system of Tandem(parallel) cycle showed the best heating performance, while the discharge temperature of refrigerant was too high. In case of the system of two stage cycle, the performance characteristics were significantly improved by employing the inter cooler.

• 생명과학회지
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• 제21권8호
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• pp.1067-1075
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• 2011

CCDC98 단백질은 BRCA1-A 복합체를 DNA 손상 부위로 이동시킴으로써 DNA손상에 의하여 유도되는 G2/M cell cycle checkpoint에 중요한 역할을 한다고 알려져 있다. 하지만 많은 연구에도 불구하고 CCDC98 단백질의 기능에 대해서 알려진 바가 거의 없다. 본 연구는 CCDC98 단백질의 기능을 밝히고자 tandem affinity purification 방법을 수행하였다. 그 결과 Wilms tumor 1-associating protein (WTAP)을 CCDC98의 결합 단백질로 분리 동정하였다. 이들 단백질의 결합을 in vivo and in vitro binding assays를 통하여 확인하였다. 또한, CCDC98 단백질이 cyclin B1의 발현을 억제함을 확인하였는데, 이는 WTAP 단백질의 발현을 조절함으로써 이루어진다는 것을 확인하였다. 이는 CCDC98 단백질이 새로운 세포주기 조절자라는 것을 증명하는 결과이다.

• Nuclear Engineering and Technology
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• 제23권2호
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• pp.165-173
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• 1991

가압경수로의 사용후핵연료를 CANDU 원자로에 재순환시키는, 이른바, 탄뎀 핵연료주기가 본 연구에서 다루어졌다. 이러한 방식으로 가압경수로의 사용후핵연료를 활용하는 것은 우라늄자원의 이용을 개선시킬뿐만 아니라 사용후핵연료 저장능력의 부족도 다소 해결할 수 있을 것이다. 핵연료를 재순환 시키는데 있어서는 CANDU 원자로의 수정을 최소화하는 방향으로 연구가 진행되었으며 본 연구에서는 9종의 핵연료가 고려되었다. 탄뎀 핵연료는 크게 핵연료재가공과 노심재구성의 두 분야로 나뉘어지는데, 핵연료 재가공의 경우, 가압경수로의 사용후핵연료는 처리되고 현재의 37 봉형 격자구조인 핵연료 다발에 맞도록 다시 성형가공되며 노심재구성의 경우, 가압경수로 사용후핵연료는 단지 격자 구조를 해체하고 CANDU의 격자길이에 맞춰 재구성만 된다. 각 탄뎀 핵연료 옵션에 대하여, 허용연소도와 출력분포를 계산하기 위해 노심연소계산이 수행되었다. 또한 경제성에 대한 접근으로 각 핵연료 옵션에 대한 핵주기비가 계산되었다. 그 결과 본 연구에서 다루어진 대부분의 탄뎀 핵연료 옵션이 경제성이 있었을 뿐만 아니라 기술적인 타당성이 있었다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.213.1-213.1
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• 2003

Sphingolipids and their metabolites are highly bioactive molecules that affect various cellular functions including differentiation, cellular senescence, apoptosis, and proliferation when added exogenously, or elevated intracellularly by turnover of complex sphingolipids or synthesis from de novo pathway. We are investigating the relationship of sphingolipids cycle in apoptosis early events. A new column liquid chromatography- tandem mass spectrometry (LC/MS/MS) in combination with multiple reaction monitoring (MRM) method was developed for the rapid, simultaneous and quantitative determination of unambiguous detecting sphingolipids in cells. (omitted)

• Journal of Microbiology and Biotechnology
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• 제18권4호
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• pp.631-638
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• 2008

Tandem affinity purification (TAP) method combined with LC-MS/MS is the most accurate and reliable way to study the interaction of proteins or proteomics in a genome-wide scale. For the first time, we used a TAP-tag as a mutagenic tool to disrupt protein interactions at the specific site. Although lots of commonly used mutational tools exist to study functions of a gene, such as deletional mutations and site-directed mutagenesis, each method has its own demerit. To test the usefulness of a TAP-tag as a mutagenic tool, we applied a TAP-tag to RNA polymerase II, which is the key enzyme of gene expression and is controlled by hundreds of transcription factors even to transcribe a gene. Our experiment is based on the hypothesis that there will be interrupted interactions between Pol II and transcription factors owing to the TAP-tag attached at the C-terminus of each subunit of Pol II, and the abnormality caused by interrupted protein interactions can be observed by measuring a cell-cycle of each yeast strain. From ten different TAP-tagged strains, Rpb7- and Rpb12-TAP-tagged strains show severe defects in growth rate and morphology. Without a heterodimer of Rpb4/Rpb7, only the ten subunits Pol II can conduct transcription normally, and there is no previously known function of Rpb7. The observed defect of the Rpb7-TAP-tagged strain shows that Rpb7 forms a complex with other proteins or compounds and the interruption of the interaction can interfere with the normal cell cycle and morphology of the cell and nucleus. This is a novel attempt to use a TAP-tag as a proteomic tool to study protein interactions.

• Nuclear Engineering and Technology
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• 제20권3호
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• pp.165-169
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• 1988

Tandem 핵연료 주기에 관한 연구의 일부로써 CANDU 원자로에 활용할 수 있는 우라늄과 플루토륨의 양을 추정하기 위해 국내 가압경수형 원자력 발전로에서 발생되는 사용후 핵연료속의 이들 핵종의 잔존량을 ORIGEN2 코드를 사용하여 각 호기별 각 batch별로 계산하여 연도별 발생량과 누적량을 구하였다. 1호기부터 10호기까지의 가압경수형 원자력 발전소를 대상으로 하였다. 계산결과 0.7내지 0.8w/o의 U-235가 주종을 이루며 또한 핵분열성 플루토늄도 상당량 배출되고 있다. 이것은 처음에 예상했던 0.8내지 0.9w/o보다 적은 값인데 이는 한전에서 제공한 연소도가 일반적인 경우보다 다소 높은 값을 나타내기 때문이다.

• Nuclear Engineering and Technology
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• 제38권4호
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• pp.359-374
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• 2006

Since 1991, Korea, Canada and United States have performed the direct use of spent pressurized water reactor (PWR) fuel in the Canada deuterium uranium (CANDU) reactors (DUPIC) fuel development project. Unlike the Tandem fuel cycle, which requires a wet reprocessing, the DUPIC fuel technology can directly refabricate CANDU fuels from the PWR spent fuel and, therefore, is recognized as a highly proliferation-resistant fuel cycle technology, which can be adopted even in non-proliferation treaty countries. The Korea Atomic Energy Research Institute (KAERI) has fabricated DUPIC fuel elements in a laboratory-scale remote fuel fabrication facility. KAERI has demonstrated the fuel performance in the research reactor, and has confirmed the operational feasibility and safety of a CANDU reactor loaded with the DUPIC fuel using conventional design and analysis tools, which will be the foundation of the future practical and commercial uses of DUPIC fuel.

• Journal of Welding and Joining
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• 제32권4호
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• pp.1-9
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• 2014

A quantitative understanding on the effect of the welding conditions on weld joint dimensions and weld thermal cycle is difficult through experimental studies alone. The experimental realization of temperature distribution in the weld pool is proved to be extremely difficult due to the small size of welds, high peak temperature and steep temperature gradients in weld pool. This review deals with the heat transfer and fluid flow analysis to understand the parametric influence of a single wire submerged arc welding (SAW) and multi-wire SAW processes on the weld bead dimensions, temperature and fluid flow distribution in the weldment.

• 대한유전성대사질환학회지
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• 제16권2호
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• pp.62-69
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• 2016

Newborn screening of some urea cycle disorders has little benefits because of early severe symptoms before the result, low sensitivity (especially hypocitrullinemia) and poor prognosis. But in case of citrullinemia, citrin deficiency and argininosuccinic aciduria diagnosed as elevated citrulline, newborn screening is helpful for early diagnosis and treatment before the symptom. Distinction between the clinical forms of these diseases is based on clinical findings and biochemical results, however, they may not be clearcut. Treatment is different from each other, so exact diagnosis is essential. Here, the diagnostic algorithm for elevated citrulline after tandem mass screening has been proposed. Minimizing total process time from sampling to report of the results is important in Korea for diagnosis and treatment of these disorders.

• Clinical and Experimental Pediatrics
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• 제49권11호
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• pp.1125-1139
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• 2006

In 1991, the Ministry of Health & Social affairs adopted a nationwide service program for neonatal screening of phenylketonuria, galactosemia, maple syrup urine disease, homocystinuria, histidinemia & congenital hypothyroidism for newborns delivered from low class pregnant women registered in health centers. Government decreased the test items from six to two, PKU & congenital hypothyroidism to increase test numbers with same budget from 1995. Government decided to test PKU & hypothyroidism for all newborns from 1997. 78 laboratories wanted to participate for neonatal screening test in 1999. Government didn't decide laboratory center for a certain district and placed responsibility on free competition. Government are planning to test 573,000 newborns from 1998, Government decided to screen 6 items PKU, congenital hypothyroidism, maple syrup urine disese, homocystinuria, galactosemia and congenital adrenal hyperplasia from 2006. 17 laboratores are participating now. The cost of screening test is supported by both the federal government and local government on a 40-60 basis. In case a patient with an inherited metabolic disease is diagnosed by screening of government program, special milk is provided at government's expense. Interlaboratory quality control was started 6 times a year from 1994. According to the government project, 3,707,773 newborns were screened. 86 PKU, 718 congenital hypothyroidism were detected. So incidence of PKU is 1/43,114 and congenital hypothyroidism is 1/4,612. Maeil dairy company produced new special formula for PKU, MMA and PA, MSUD, urea cycle disorder, homocystinuria, isovaleric acidemia from Oct. 1999. The cost benefit of performing screening procedures coupled with treatment has been estimated to be as high as 1.77 times in PKU, 11.11 times in congenital hypothyroidism than cost without screening. We are trying to increase the budget to test all newborns for Tandem mass sereening & Wilson disease from 2008. Now it is a very important problem to decrease laboratory numbers of neonatal screening in Korea. So we are considering 4-5 central laboratories which cover all newborns and are equipped with tandem mass spectrometer & enzyme immunoassay for TSH, 17OHP & enzyme colorimetric assay for galactose.