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http://dx.doi.org/10.5352/JLS.2011.21.8.1067

Coiled-Coil Domain-Containing Protein 98 (CCDC98) Regulates Cyclin B1 Expression by Affecting WTAP Protein Stability  

Oh, Yun-Jung (Department of Biological Sciences, Sungkyunkwan University)
Lee, Eun-Hee (Department of Biological Sciences, Sungkyunkwan University)
Lee, Il-Kyu (Department of Family Medicine, The Catholic University of Korea)
Kim, Kyung-Soo (Department of Family Medicine, The Catholic University of Korea)
Kim, Hong-Tae (Department of Biological Sciences, Sungkyunkwan University)
Publication Information
Journal of Life Science / v.21, no.8, 2011 , pp. 1067-1075 More about this Journal
Abstract
Coiled-coil domain-containing protein 98 (CCDC98) plays a role in G2/M DNA damage checkpoint pathways by recruiting breast cancer 1 (BRCA1)-A complex to the DNA-damaged sites. However, the molecular mechanism of CCDC98 on the DNA damage-induced G2/M checkpoint pathways is unclear. In this study, we identifed Wilms tumor 1-associating protein (WTAP) as a novel CCDC98-binding protein, using tandem affinity purification. We confirmed the association between CCDC98 and WTAP using in vivo and in vitro binding assays. We demonstrated that CCDC98 regulates cyclin B1 expression by affecting WTAP protein stability. Based on these results, we suggest that CCDC98 may act as a novel cell cycle regulator by regulating the expression level of cyclin B1.
Keywords
DNA damage; Coiled-coil domain-containing protein 98 (CCDC98); Wilms tumor 1-associating protein (WTAP); cyclin B1; cell cycle;
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